SK12212000A3 - Metóda na detekciu cieľovej nukleovej kyseliny pomocou PCR - Google Patents
Metóda na detekciu cieľovej nukleovej kyseliny pomocou PCR Download PDFInfo
- Publication number
- SK12212000A3 SK12212000A3 SK1221-2000A SK12212000A SK12212000A3 SK 12212000 A3 SK12212000 A3 SK 12212000A3 SK 12212000 A SK12212000 A SK 12212000A SK 12212000 A3 SK12212000 A3 SK 12212000A3
- Authority
- SK
- Slovakia
- Prior art keywords
- probe
- sequence
- amplification
- fluorescence
- sample
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 62
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 24
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 15
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 15
- 238000001514 detection method Methods 0.000 title description 10
- 239000000523 sample Substances 0.000 claims abstract description 132
- 230000003321 amplification Effects 0.000 claims abstract description 74
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 74
- 238000006243 chemical reaction Methods 0.000 claims abstract description 60
- 239000002773 nucleotide Substances 0.000 claims abstract description 46
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 46
- 238000012544 monitoring process Methods 0.000 claims abstract description 14
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 10
- 108020004414 DNA Proteins 0.000 claims description 24
- 238000003752 polymerase chain reaction Methods 0.000 claims description 21
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 12
- 238000009396 hybridization Methods 0.000 claims description 11
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims description 10
- 108090000790 Enzymes Proteins 0.000 claims description 9
- 102000004190 Enzymes Human genes 0.000 claims description 9
- 102000053602 DNA Human genes 0.000 claims description 5
- 108091033319 polynucleotide Proteins 0.000 claims description 5
- 102000040430 polynucleotide Human genes 0.000 claims description 5
- 239000002157 polynucleotide Substances 0.000 claims description 5
- 229940035893 uracil Drugs 0.000 claims description 5
- 239000000975 dye Substances 0.000 claims description 4
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- 102000054765 polymorphisms of proteins Human genes 0.000 claims description 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 3
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- 108010068698 spleen exonuclease Proteins 0.000 claims 1
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 16
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- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 230000001419 dependent effect Effects 0.000 description 4
- 238000001228 spectrum Methods 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
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- 230000003993 interaction Effects 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 108091026890 Coding region Proteins 0.000 description 2
- 108060002716 Exonuclease Proteins 0.000 description 2
- 108091092195 Intron Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 230000001186 cumulative effect Effects 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 102000013165 exonuclease Human genes 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 2
- 238000009830 intercalation Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 230000002112 DNA intercalation Effects 0.000 description 1
- QTANTQQOYSUMLC-UHFFFAOYSA-O Ethidium cation Chemical compound C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 QTANTQQOYSUMLC-UHFFFAOYSA-O 0.000 description 1
- 108700024394 Exon Proteins 0.000 description 1
- 241000725303 Human immunodeficiency virus Species 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 108091093037 Peptide nucleic acid Proteins 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 102000006943 Uracil-DNA Glycosidase Human genes 0.000 description 1
- 108010072685 Uracil-DNA Glycosidase Proteins 0.000 description 1
- 238000005903 acid hydrolysis reaction Methods 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 150000001649 bromium compounds Chemical group 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 108091036078 conserved sequence Proteins 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000001687 destabilization Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000005518 electrochemistry Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 230000007274 generation of a signal involved in cell-cell signaling Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000002687 intercalation Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000037452 priming Effects 0.000 description 1
- 230000002250 progressing effect Effects 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/80—Elimination or reduction of contamination by undersired ferments, e.g. aseptic cultivation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Eye Examination Apparatus (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Geophysics And Detection Of Objects (AREA)
- Investigating Or Analyzing Materials By The Use Of Fluid Adsorption Or Reactions (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9803382.2A GB9803382D0 (en) | 1998-02-19 | 1998-02-19 | Detection system |
| PCT/GB1999/000504 WO1999042611A1 (en) | 1998-02-19 | 1999-02-18 | Method for detection of target nucleic acids using pcr |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SK12212000A3 true SK12212000A3 (sk) | 2002-09-10 |
Family
ID=10827164
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SK1221-2000A SK12212000A3 (sk) | 1998-02-19 | 1999-02-18 | Metóda na detekciu cieľovej nukleovej kyseliny pomocou PCR |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US6287781B1 (ru) |
| EP (1) | EP1055002B1 (ru) |
| JP (1) | JP2003512808A (ru) |
| CN (1) | CN1203186C (ru) |
| AT (1) | ATE398187T1 (ru) |
| AU (1) | AU746149B2 (ru) |
| CA (1) | CA2321185C (ru) |
| CZ (1) | CZ20002958A3 (ru) |
| DE (1) | DE69938894D1 (ru) |
| GB (4) | GB9803382D0 (ru) |
| HU (1) | HU226518B1 (ru) |
| IL (1) | IL137909A0 (ru) |
| NZ (1) | NZ506333A (ru) |
| RU (1) | RU2199588C2 (ru) |
| SK (1) | SK12212000A3 (ru) |
| WO (1) | WO1999042611A1 (ru) |
Families Citing this family (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9725197D0 (en) * | 1997-11-29 | 1998-01-28 | Secr Defence | Detection system |
| GB9918237D0 (en) * | 1999-08-04 | 1999-10-06 | Secr Defence | Detection system |
| GB0005281D0 (en) * | 2000-03-07 | 2000-04-26 | Secr Defence | Analytical method |
| GB2360088A (en) * | 2000-03-07 | 2001-09-12 | Secr Defence | Method and kit for determining PCR amplification reaction conditions |
| AU2002227156A1 (en) | 2000-12-01 | 2002-06-11 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| GB0110501D0 (en) | 2001-04-30 | 2001-06-20 | Secr Defence Brit | Amplification process |
| DE60228952D1 (de) * | 2001-05-07 | 2008-10-30 | Mayo Foundation | Nachweis von Legionella durch ein PCR und FRET verwendendes Verfahren |
| GB0111275D0 (en) | 2001-05-09 | 2001-06-27 | Secr Defence | Analytical method and kit |
| GB0112868D0 (en) * | 2001-05-25 | 2001-07-18 | Secr Defence | Detection system |
| US9261460B2 (en) | 2002-03-12 | 2016-02-16 | Enzo Life Sciences, Inc. | Real-time nucleic acid detection processes and compositions |
| US6593093B1 (en) * | 2002-02-20 | 2003-07-15 | Mayo Foundation For Medical Education And Research | Detection of group a Streptococcus |
| US9353405B2 (en) | 2002-03-12 | 2016-05-31 | Enzo Life Sciences, Inc. | Optimized real time nucleic acid detection processes |
| WO2003102179A1 (fr) * | 2002-05-31 | 2003-12-11 | Kankyo Engineering Co., Ltd. | Nouveau procede de dosage d'acide nucleique au moyen d'un nucleotide marque |
| US20070059690A1 (en) * | 2002-05-31 | 2007-03-15 | Amirul Islam | "Met/fret based method of target nucleic acid detection whereby the donor/acceptor moieties are on complementary strands" |
| US7183104B1 (en) | 2002-08-23 | 2007-02-27 | Duane Morris Llp | Separator and particle detection system |
| US7074598B2 (en) * | 2002-09-25 | 2006-07-11 | Mayo Foundation For Medical Education And Research | Detection of vancomycin-resistant enterococcus spp. |
| WO2004074447A2 (en) * | 2003-02-18 | 2004-09-02 | Applera Corporation | Compositions and methods for multiplex analysis of polynucleotides |
| GB0304832D0 (en) | 2003-03-04 | 2003-04-09 | Secr Defence | Assay method |
| WO2005003388A2 (en) * | 2003-06-30 | 2005-01-13 | Astrazeneca Ab | Fluorescence assays for nucleic acid polymerase activity |
| GB0416944D0 (en) * | 2004-07-29 | 2004-09-01 | Lumora Ltd | Method for determining the amount of template nucleic acid present in a sample |
| GB0503172D0 (en) | 2005-02-16 | 2005-03-23 | Enigma Diagnostics Ltd | Detection method |
| GB0517005D0 (en) | 2005-08-19 | 2005-09-28 | Enigma Diagnostics Ltd | Analytical method and kit |
| RU2005132940A (ru) * | 2006-03-01 | 2007-11-27 | Институт биохимии и генетики Уфимского научного центра РАН (RU) | Способ детекции специфических фрагментов днк или рнк с помощью полимеразной цепной реакции в режиме реального времени |
| EP2759307B1 (en) | 2006-03-29 | 2016-11-09 | Merial Limited | Vaccine against Streptococci |
| GB0806676D0 (en) | 2008-04-12 | 2008-05-14 | Environment Agency The | Environmental monitoring |
| EP2116614A1 (en) * | 2008-05-06 | 2009-11-11 | Qiagen GmbH | Simultaneous detection of multiple nucleic acid sequences in a reaction |
| EP2138588A1 (en) * | 2008-06-23 | 2009-12-30 | Koninklijke Philips Electronics N.V. | Melting curve measurement during amplification |
| WO2011022589A2 (en) * | 2009-08-20 | 2011-02-24 | Mayo Foundation For Medical Education And Research | Detection of enterovirus |
| GB0915664D0 (en) | 2009-09-08 | 2009-10-07 | Enigma Diagnostics Ltd | Reaction method |
| RU2585519C2 (ru) * | 2012-11-28 | 2016-05-27 | ЗАО "Геноаналитика" | Способ селективного отбора целевых участков днк |
| WO2016049657A1 (en) * | 2014-09-26 | 2016-03-31 | Two Pore Guys, Inc. | Target sequence detection by nanopore sensing of synthetic probes |
| US11486873B2 (en) | 2016-03-31 | 2022-11-01 | Ontera Inc. | Multipore determination of fractional abundance of polynucleotide sequences in a sample |
| RU2665631C2 (ru) * | 2017-01-25 | 2018-09-03 | Общество с ограниченной ответственностью "Секвойя Дженетикс" | Способ специфической идентификации последовательностей днк |
| CN108642150B (zh) * | 2018-05-18 | 2023-12-08 | 阅尔基因技术（苏州）有限公司 | 一种基于fret的荧光核酸检测方法和试剂盒 |
| US11604186B2 (en) * | 2018-10-17 | 2023-03-14 | Molecular Devices (Austria) GmbH | Real time western blot assays utilizing fluorescence resonance energy transfer (FRET) |
| BR112020026394A2 (pt) | 2018-12-21 | 2021-03-23 | Illumina, Inc. | método, sistema e aparelho de detecção |
| CN109576350B (zh) * | 2019-01-18 | 2021-01-29 | 深圳恒特基因有限公司 | 一种dna与rna同时定量的试剂盒、方法及质控方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5567583A (en) | 1991-12-16 | 1996-10-22 | Biotronics Corporation | Methods for reducing non-specific priming in DNA detection |
| DE69610304T2 (de) | 1995-05-05 | 2001-04-05 | Perkin Elmer Corp | Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay |
| US5716784A (en) | 1996-02-05 | 1998-02-10 | The Perkin-Elmer Corporation | Fluorescence detection assay for homogeneous PCR hybridization systems |
| PT1179600E (pt) | 1996-06-04 | 2005-08-31 | Univ Utah Res Found | Monitorizacao da hibridacao durante a rcp |
| US5866336A (en) * | 1996-07-16 | 1999-02-02 | Oncor, Inc. | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| US6140054A (en) * | 1998-09-30 | 2000-10-31 | University Of Utah Research Foundation | Multiplex genotyping using fluorescent hybridization probes |
-
1998
- 1998-02-19 GB GBGB9803382.2A patent/GB9803382D0/en not_active Ceased
-
1999
- 1999-02-03 GB GBGB9902290.7A patent/GB9902290D0/en not_active Ceased
- 1999-02-18 US US09/622,426 patent/US6287781B1/en not_active Expired - Lifetime
- 1999-02-18 AT AT99905085T patent/ATE398187T1/de not_active IP Right Cessation
- 1999-02-18 IL IL13790999A patent/IL137909A0/xx not_active IP Right Cessation
- 1999-02-18 HU HU0101385A patent/HU226518B1/hu not_active IP Right Cessation
- 1999-02-18 JP JP2000532551A patent/JP2003512808A/ja active Pending
- 1999-02-18 GB GBGB9903578.4A patent/GB9903578D0/en not_active Ceased
- 1999-02-18 NZ NZ506333A patent/NZ506333A/xx unknown
- 1999-02-18 SK SK1221-2000A patent/SK12212000A3/sk unknown
- 1999-02-18 WO PCT/GB1999/000504 patent/WO1999042611A1/en active IP Right Grant
- 1999-02-18 DE DE69938894T patent/DE69938894D1/de not_active Expired - Lifetime
- 1999-02-18 CN CNB998051985A patent/CN1203186C/zh not_active Expired - Fee Related
- 1999-02-18 CZ CZ20002958A patent/CZ20002958A3/cs unknown
- 1999-02-18 CA CA002321185A patent/CA2321185C/en not_active Expired - Fee Related
- 1999-02-18 EP EP99905085A patent/EP1055002B1/en not_active Expired - Lifetime
- 1999-02-18 RU RU2000124099/13A patent/RU2199588C2/ru active
- 1999-02-18 AU AU25383/99A patent/AU746149B2/en not_active Ceased
- 1999-02-19 GB GBGB9903796.2A patent/GB9903796D0/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| ATE398187T1 (de) | 2008-07-15 |
| HU226518B1 (en) | 2009-03-30 |
| CA2321185C (en) | 2009-04-21 |
| EP1055002B1 (en) | 2008-06-11 |
| GB9903796D0 (en) | 1999-04-14 |
| US6287781B1 (en) | 2001-09-11 |
| JP2003512808A (ja) | 2003-04-08 |
| GB9803382D0 (en) | 1998-04-15 |
| DE69938894D1 (de) | 2008-07-24 |
| GB9903578D0 (en) | 1999-04-07 |
| CN1203186C (zh) | 2005-05-25 |
| AU746149B2 (en) | 2002-04-18 |
| HUP0101385A3 (en) | 2003-08-28 |
| EP1055002A1 (en) | 2000-11-29 |
| CN1297488A (zh) | 2001-05-30 |
| IL137909A0 (en) | 2001-10-31 |
| CZ20002958A3 (cs) | 2002-02-13 |
| RU2199588C2 (ru) | 2003-02-27 |
| GB9902290D0 (en) | 1999-03-24 |
| AU2538399A (en) | 1999-09-06 |
| WO1999042611A1 (en) | 1999-08-26 |
| HUP0101385A2 (hu) | 2001-08-28 |
| CA2321185A1 (en) | 1999-08-26 |
| NZ506333A (en) | 2002-10-25 |
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