SG178346A1 - Monoclonal antibodies against the pbp2-a protein and homologous sequences for the treatment of infections by and immunodiagnostics of bacteria of the firmicutes phylum - Google Patents
Monoclonal antibodies against the pbp2-a protein and homologous sequences for the treatment of infections by and immunodiagnostics of bacteria of the firmicutes phylum Download PDFInfo
- Publication number
- SG178346A1 SG178346A1 SG2012009205A SG2012009205A SG178346A1 SG 178346 A1 SG178346 A1 SG 178346A1 SG 2012009205 A SG2012009205 A SG 2012009205A SG 2012009205 A SG2012009205 A SG 2012009205A SG 178346 A1 SG178346 A1 SG 178346A1
- Authority
- SG
- Singapore
- Prior art keywords
- seq
- sequence
- pbp2a
- mrsa
- bacteria
- Prior art date
Links
- 241000894006 Bacteria Species 0.000 title claims abstract description 119
- 208000015181 infectious disease Diseases 0.000 title claims abstract description 75
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 50
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 43
- 238000011282 treatment Methods 0.000 title claims abstract description 28
- 241000192125 Firmicutes Species 0.000 title abstract description 4
- 241000194033 Enterococcus Species 0.000 claims abstract description 14
- 241001465754 Metazoa Species 0.000 claims description 102
- 101710202686 Penicillin-sensitive transpeptidase Proteins 0.000 claims description 77
- 230000001580 bacterial effect Effects 0.000 claims description 36
- 238000000034 method Methods 0.000 claims description 15
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 11
- 150000003952 β-lactams Chemical class 0.000 claims description 11
- 239000004599 antimicrobial Substances 0.000 claims description 10
- 230000002265 prevention Effects 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 230000007850 degeneration Effects 0.000 claims 10
- 150000007523 nucleic acids Chemical group 0.000 claims 6
- 239000002773 nucleotide Substances 0.000 claims 6
- 125000003729 nucleotide group Chemical group 0.000 claims 6
- 125000003275 alpha amino acid group Chemical group 0.000 claims 4
- 238000003745 diagnosis Methods 0.000 claims 1
- 239000003937 drug carrier Substances 0.000 claims 1
- 239000008194 pharmaceutical composition Substances 0.000 claims 1
- 239000000546 pharmaceutical excipient Substances 0.000 claims 1
- 238000012360 testing method Methods 0.000 abstract description 33
- 241000191967 Staphylococcus aureus Species 0.000 abstract description 28
- 244000052769 pathogen Species 0.000 abstract description 15
- 241000192097 Staphylococcus sciuri Species 0.000 abstract description 5
- 230000000295 complement effect Effects 0.000 abstract description 5
- 239000003782 beta lactam antibiotic agent Substances 0.000 abstract description 4
- 239000002132 β-lactam antibiotic Substances 0.000 abstract description 4
- 229940124586 β-lactam antibiotics Drugs 0.000 abstract description 4
- MYPYJXKWCTUITO-LYRMYLQWSA-N vancomycin Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C2C=C3C=C1OC1=CC=C(C=C1Cl)[C@@H](O)[C@H](C(N[C@@H](CC(N)=O)C(=O)N[C@H]3C(=O)N[C@H]1C(=O)N[C@H](C(N[C@@H](C3=CC(O)=CC(O)=C3C=3C(O)=CC=C1C=3)C(O)=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)O2)=O)NC(=O)[C@@H](CC(C)C)NC)[C@H]1C[C@](C)(N)[C@H](O)[C@H](C)O1 MYPYJXKWCTUITO-LYRMYLQWSA-N 0.000 description 55
- 108010059993 Vancomycin Proteins 0.000 description 53
- 229960003165 vancomycin Drugs 0.000 description 53
- MYPYJXKWCTUITO-UHFFFAOYSA-N vancomycin Natural products O1C(C(=C2)Cl)=CC=C2C(O)C(C(NC(C2=CC(O)=CC(O)=C2C=2C(O)=CC=C3C=2)C(O)=O)=O)NC(=O)C3NC(=O)C2NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(CC(C)C)NC)C(O)C(C=C3Cl)=CC=C3OC3=CC2=CC1=C3OC1OC(CO)C(O)C(O)C1OC1CC(C)(N)C(O)C(C)O1 MYPYJXKWCTUITO-UHFFFAOYSA-N 0.000 description 53
- 238000003556 assay Methods 0.000 description 39
- 238000011002 quantification Methods 0.000 description 30
- 239000002054 inoculum Substances 0.000 description 26
- 230000009885 systemic effect Effects 0.000 description 23
- 210000003734 kidney Anatomy 0.000 description 21
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 19
- 239000002953 phosphate buffered saline Substances 0.000 description 19
- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 description 18
- 238000007912 intraperitoneal administration Methods 0.000 description 18
- 229960003085 meticillin Drugs 0.000 description 18
- 230000009467 reduction Effects 0.000 description 17
- 206010041925 Staphylococcal infections Diseases 0.000 description 15
- 239000000523 sample Substances 0.000 description 15
- 102000004190 Enzymes Human genes 0.000 description 14
- 108090000790 Enzymes Proteins 0.000 description 14
- 238000000338 in vitro Methods 0.000 description 14
- 208000015688 methicillin-resistant staphylococcus aureus infectious disease Diseases 0.000 description 13
- 238000002965 ELISA Methods 0.000 description 12
- 108090000279 Peptidyltransferases Proteins 0.000 description 12
- 238000001727 in vivo Methods 0.000 description 12
- 230000004083 survival effect Effects 0.000 description 12
- 108010004729 Phycoerythrin Proteins 0.000 description 11
- 229940079593 drug Drugs 0.000 description 11
- 239000003814 drug Substances 0.000 description 11
- 230000003053 immunization Effects 0.000 description 11
- 238000002649 immunization Methods 0.000 description 11
- 231100000636 lethal dose Toxicity 0.000 description 11
- 150000001413 amino acids Chemical class 0.000 description 10
- 238000003119 immunoblot Methods 0.000 description 10
- 238000010172 mouse model Methods 0.000 description 10
- 239000013642 negative control Substances 0.000 description 10
- 230000001717 pathogenic effect Effects 0.000 description 10
- 210000002966 serum Anatomy 0.000 description 10
- 239000006228 supernatant Substances 0.000 description 10
- 206010011409 Cross infection Diseases 0.000 description 9
- 108010041986 DNA Vaccines Proteins 0.000 description 8
- 206010015548 Euthanasia Diseases 0.000 description 8
- 241000699670 Mus sp. Species 0.000 description 8
- 238000000684 flow cytometry Methods 0.000 description 8
- 239000012634 fragment Substances 0.000 description 8
- 238000011534 incubation Methods 0.000 description 8
- 108010047041 Complementarity Determining Regions Proteins 0.000 description 7
- 229940021995 DNA vaccine Drugs 0.000 description 7
- 230000003321 amplification Effects 0.000 description 7
- 230000000845 anti-microbial effect Effects 0.000 description 7
- 238000010790 dilution Methods 0.000 description 7
- 239000012895 dilution Substances 0.000 description 7
- 210000004408 hybridoma Anatomy 0.000 description 7
- 230000001524 infective effect Effects 0.000 description 7
- 238000003199 nucleic acid amplification method Methods 0.000 description 7
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 7
- 108020004414 DNA Proteins 0.000 description 6
- 229920001213 Polysorbate 20 Polymers 0.000 description 6
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 6
- SOIFLUNRINLCBN-UHFFFAOYSA-N ammonium thiocyanate Chemical compound [NH4+].[S-]C#N SOIFLUNRINLCBN-UHFFFAOYSA-N 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 6
- 239000004202 carbamide Substances 0.000 description 6
- MYPYJXKWCTUITO-KIIOPKALSA-N chembl3301825 Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C2C=C3C=C1OC1=CC=C(C=C1Cl)[C@@H](O)[C@H](C(N[C@@H](CC(N)=O)C(=O)N[C@H]3C(=O)N[C@H]1C(=O)N[C@H](C(N[C@H](C3=CC(O)=CC(O)=C3C=3C(O)=CC=C1C=3)C(O)=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)O2)=O)NC(=O)[C@@H](CC(C)C)NC)[C@H]1C[C@](C)(N)C(O)[C@H](C)O1 MYPYJXKWCTUITO-KIIOPKALSA-N 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 230000028993 immune response Effects 0.000 description 6
- 230000002401 inhibitory effect Effects 0.000 description 6
- 239000012528 membrane Substances 0.000 description 6
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 6
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- 230000002829 reductive effect Effects 0.000 description 6
- 241000194031 Enterococcus faecium Species 0.000 description 5
- 241000191940 Staphylococcus Species 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 238000004113 cell culture Methods 0.000 description 5
- 239000000499 gel Substances 0.000 description 5
- 238000004128 high performance liquid chromatography Methods 0.000 description 5
- 238000003018 immunoassay Methods 0.000 description 5
- 229920002401 polyacrylamide Polymers 0.000 description 5
- 239000013641 positive control Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 230000001681 protective effect Effects 0.000 description 5
- 238000012163 sequencing technique Methods 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 230000005526 G1 to G0 transition Effects 0.000 description 4
- 108010015899 Glycopeptides Proteins 0.000 description 4
- 102000002068 Glycopeptides Human genes 0.000 description 4
- 239000006137 Luria-Bertani broth Substances 0.000 description 4
- 239000000427 antigen Substances 0.000 description 4
- 102000036639 antigens Human genes 0.000 description 4
- 108091007433 antigens Proteins 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 4
- 230000004927 fusion Effects 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 231100000518 lethal Toxicity 0.000 description 4
- 230000001665 lethal effect Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 108700028369 Alleles Proteins 0.000 description 3
- 241001495410 Enterococcus sp. Species 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- 241001529936 Murinae Species 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 238000010367 cloning Methods 0.000 description 3
- 239000002299 complementary DNA Substances 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 238000010494 dissociation reaction Methods 0.000 description 3
- 230000005593 dissociations Effects 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 238000009650 gentamicin protection assay Methods 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 210000000987 immune system Anatomy 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 238000010841 mRNA extraction Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000012521 purified sample Substances 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 229940124513 senna glycoside Drugs 0.000 description 3
- 239000007858 starting material Substances 0.000 description 3
- 229960005486 vaccine Drugs 0.000 description 3
- NFGXHKASABOEEW-UHFFFAOYSA-N 1-methylethyl 11-methoxy-3,7,11-trimethyl-2,4-dodecadienoate Chemical compound COC(C)(C)CCCC(C)CC=CC(C)=CC(=O)OC(C)C NFGXHKASABOEEW-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- 208000035143 Bacterial infection Diseases 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 235000006693 Cassia laevigata Nutrition 0.000 description 2
- 241000194032 Enterococcus faecalis Species 0.000 description 2
- 102100034343 Integrase Human genes 0.000 description 2
- 206010029155 Nephropathy toxic Diseases 0.000 description 2
- 206010033109 Ototoxicity Diseases 0.000 description 2
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 2
- 241000522641 Senna Species 0.000 description 2
- 208000019802 Sexually transmitted disease Diseases 0.000 description 2
- 241000295644 Staphylococcaceae Species 0.000 description 2
- ZMZDMBWJUHKJPS-UHFFFAOYSA-M Thiocyanate anion Chemical compound [S-]C#N ZMZDMBWJUHKJPS-UHFFFAOYSA-M 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000012298 atmosphere Substances 0.000 description 2
- 208000022362 bacterial infectious disease Diseases 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000024203 complement activation Effects 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 231100000517 death Toxicity 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- ZMZDMBWJUHKJPS-UHFFFAOYSA-N hydrogen thiocyanate Natural products SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 238000007918 intramuscular administration Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 231100000417 nephrotoxicity Toxicity 0.000 description 2
- 230000007694 nephrotoxicity Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 230000014207 opsonization Effects 0.000 description 2
- 231100000262 ototoxicity Toxicity 0.000 description 2
- UWYHMGVUTGAWSP-JKIFEVAISA-N oxacillin Chemical compound N([C@@H]1C(N2[C@H](C(C)(C)S[C@@H]21)C(O)=O)=O)C(=O)C1=C(C)ON=C1C1=CC=CC=C1 UWYHMGVUTGAWSP-JKIFEVAISA-N 0.000 description 2
- 229960001019 oxacillin Drugs 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 230000001018 virulence Effects 0.000 description 2
- 230000007923 virulence factor Effects 0.000 description 2
- 239000000304 virulence factor Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- NVKAWKQGWWIWPM-ABEVXSGRSA-N 17-β-hydroxy-5-α-Androstan-3-one Chemical compound C1C(=O)CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CC[C@H]21 NVKAWKQGWWIWPM-ABEVXSGRSA-N 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- 206010003497 Asphyxia Diseases 0.000 description 1
- 238000011725 BALB/c mouse Methods 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 101710116957 D-alanyl-D-alanine carboxypeptidase Proteins 0.000 description 1
- 238000011238 DNA vaccination Methods 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 208000032163 Emerging Communicable disease Diseases 0.000 description 1
- 101001013832 Homo sapiens Mitochondrial peptide methionine sulfoxide reductase Proteins 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 208000022010 Lhermitte-Duclos disease Diseases 0.000 description 1
- 239000012515 MabSelect SuRe Substances 0.000 description 1
- 208000037942 Methicillin-resistant Staphylococcus aureus infection Diseases 0.000 description 1
- 102100031767 Mitochondrial peptide methionine sulfoxide reductase Human genes 0.000 description 1
- MSFSPUZXLOGKHJ-UHFFFAOYSA-N Muraminsaeure Natural products OC(=O)C(C)OC1C(N)C(O)OC(CO)C1O MSFSPUZXLOGKHJ-UHFFFAOYSA-N 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 206010029803 Nosocomial infection Diseases 0.000 description 1
- 108010078471 Panton-Valentine leukocidin Proteins 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 108700020474 Penicillin-Binding Proteins Proteins 0.000 description 1
- 108010013639 Peptidoglycan Proteins 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 238000011530 RNeasy Mini Kit Methods 0.000 description 1
- 231100000645 Reed–Muench method Toxicity 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 206010066409 Staphylococcal skin infection Diseases 0.000 description 1
- 108010053950 Teicoplanin Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000002924 anti-infective effect Effects 0.000 description 1
- 238000009635 antibiotic susceptibility testing Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000011545 carbonate/bicarbonate buffer Substances 0.000 description 1
- 230000007910 cell fusion Effects 0.000 description 1
- DDTDNCYHLGRFBM-YZEKDTGTSA-N chembl2367892 Chemical compound CC(=O)N[C@H]1[C@@H](O)[C@H](O)[C@H](CO)O[C@H]1O[C@@H]([C@H]1C(N[C@@H](C2=CC(O)=CC(O[C@@H]3[C@H]([C@H](O)[C@H](O)[C@@H](CO)O3)O)=C2C=2C(O)=CC=C(C=2)[C@@H](NC(=O)[C@@H]2NC(=O)[C@@H]3C=4C=C(O)C=C(C=4)OC=4C(O)=CC=C(C=4)[C@@H](N)C(=O)N[C@H](CC=4C=C(Cl)C(O5)=CC=4)C(=O)N3)C(=O)N1)C(O)=O)=O)C(C=C1Cl)=CC=C1OC1=C(O[C@H]3[C@H]([C@@H](O)[C@H](O)[C@H](CO)O3)NC(C)=O)C5=CC2=C1 DDTDNCYHLGRFBM-YZEKDTGTSA-N 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011254 conventional chemotherapy Methods 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229940032049 enterococcus faecalis Drugs 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000007499 fusion processing Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000028996 humoral immune response Effects 0.000 description 1
- 230000000521 hyperimmunizing effect Effects 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 231100000386 immunotoxicity Toxicity 0.000 description 1
- 230000007688 immunotoxicity Effects 0.000 description 1
- 238000000126 in silico method Methods 0.000 description 1
- 238000005462 in vivo assay Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 229960000598 infliximab Drugs 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 238000012792 lyophilization process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 101150008979 mecA gene Proteins 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 238000007479 molecular analysis Methods 0.000 description 1
- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- 229940022007 naked DNA vaccine Drugs 0.000 description 1
- 208000004235 neutropenia Diseases 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 230000003571 opsonizing effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 102000013415 peroxidase activity proteins Human genes 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 231100000444 skin lesion Toxicity 0.000 description 1
- 206010040882 skin lesion Diseases 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000013595 supernatant sample Substances 0.000 description 1
- 229960001608 teicoplanin Drugs 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BRPI0914508-7A BRPI0914508B1 (pt) | 2009-08-10 | 2009-08-10 | ANTICORPOS MONOCLONAIS PARA A PROTEÍNA PBP2a, E, COMPOSIÇÃO FARMACÊUTICA |
| PCT/BR2010/000263 WO2011017791A1 (pt) | 2009-08-10 | 2010-08-10 | Anticorpos monoclonais para a proteína pbp2-a e sequências homólogas para o tratamento de infeçcões e imunodiagnóstico em bactérias do filo firmicutes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG178346A1 true SG178346A1 (en) | 2012-03-29 |
Family
ID=45896895
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG2012009205A SG178346A1 (en) | 2009-08-10 | 2010-08-10 | Monoclonal antibodies against the pbp2-a protein and homologous sequences for the treatment of infections by and immunodiagnostics of bacteria of the firmicutes phylum |
Country Status (6)
| Country | Link |
|---|---|
| JP (1) | JP5785544B2 (zh) |
| CN (1) | CN102741281B (zh) |
| AU (1) | AU2010282162B2 (zh) |
| CU (2) | CU24180B1 (zh) |
| HK (1) | HK1176077A1 (zh) |
| SG (1) | SG178346A1 (zh) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105315367B (zh) * | 2014-07-29 | 2019-01-29 | 广州瑞博奥生物科技有限公司 | 用于检测耐甲氧西林金黄色葡萄球菌的酶联免疫试剂盒 |
| CN113150139B (zh) * | 2021-02-01 | 2022-09-09 | 深圳市龙华区疾病预防控制中心 | 一种PBP2a单克隆抗体及其制备方法和应用 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU5867100A (en) * | 1999-05-03 | 2000-12-12 | Medarex, Inc. | Human antibodies to staphylococcus aureus |
-
2010
- 2010-08-10 CN CN201080043628.XA patent/CN102741281B/zh active Active
- 2010-08-10 CU CU20140101A patent/CU24180B1/es active IP Right Grant
- 2010-08-10 SG SG2012009205A patent/SG178346A1/en unknown
- 2010-08-10 AU AU2010282162A patent/AU2010282162B2/en active Active
- 2010-08-10 JP JP2012524064A patent/JP5785544B2/ja active Active
- 2010-08-10 CU CU2012000026A patent/CU24095B1/es active IP Right Grant
-
2013
- 2013-03-20 HK HK13103458.4A patent/HK1176077A1/zh unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JP2013501732A (ja) | 2013-01-17 |
| JP5785544B2 (ja) | 2015-09-30 |
| CU20120026A7 (es) | 2012-06-21 |
| AU2010282162A8 (en) | 2012-05-24 |
| AU2010282162B2 (en) | 2016-06-02 |
| CU24095B1 (es) | 2015-04-29 |
| RU2012108941A (ru) | 2013-09-20 |
| HK1176077A1 (zh) | 2013-07-19 |
| CN102741281A (zh) | 2012-10-17 |
| AU2010282162A1 (en) | 2012-04-05 |
| CU24180B1 (es) | 2016-04-25 |
| CU20140101A7 (es) | 2015-01-29 |
| CN102741281B (zh) | 2015-02-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9927428B2 (en) | Gram-positive bacteria specific binding compounds | |
| US10781246B2 (en) | Compositions and methods for anti-staphylococcal biologic agents | |
| CN103906535B (zh) | 与葡萄球菌蛋白a的抗体相关的组合物和方法 | |
| US20180179267A1 (en) | Anti-staphylococcus aureus antibody combination preparation | |
| EP2476702B1 (en) | Monoclonal antibodies against the pbp2-a protein and homologous sequences for the treatment of infections by and immunodiagnostics of bacteria of the firmicutes phylum | |
| AU2010282162B2 (en) | Monoclonal antibodies against the PBP2-a protein and homologous sequences for the treatment of infections by and immunodiagnostics of bacteria of the firmicutes phylum | |
| CA2770771C (en) | Monoclonal antibodies for pbp2-a protein and homologous sequences for treatment of infections and immunodiagnosis on bacteria from phylum firmicutes | |
| US20180105584A1 (en) | Antibody directed against immunoglobulin-binding proteins of s. aureus | |
| KR101868160B1 (ko) | Ｐｂｐ２－ａ 단백질용 단클론 항체 및 문 퍼미큐트 세균에 대한 면역진단 및 감염 치료를 위한 상동 서열 | |
| RU2575070C2 (ru) | МОНОКЛОНАЛЬНЫЕ АНТИТЕЛА ПРОТИВ БЕЛКА РВР2-А И ГОМОЛОГИЧНЫХ ПОСЛЕДОВАТЕЛЬНОСТЕЙ ДЛЯ ЛЕЧЕНИЯ ЗАРАЖЕНИЯ БАКТЕРИЯМИ И ИММУНОДИАГНОСТИКИ БАКТЕРИЙ ТИПА Firmicutes | |
| Rocha | Determining the Role of Panton-Valentine Leukocidin in Community-acquired Methicillin-resistant Staphylococcus Aureus Pathogenesis |