SG11202010857TA - Preparation method for rejuvenated regenerative fibroblast and application thereof - Google Patents

Preparation method for rejuvenated regenerative fibroblast and application thereof

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Publication number
SG11202010857TA
SG11202010857TA SG11202010857TA SG11202010857TA SG11202010857TA SG 11202010857T A SG11202010857T A SG 11202010857TA SG 11202010857T A SG11202010857T A SG 11202010857TA SG 11202010857T A SG11202010857T A SG 11202010857TA SG 11202010857T A SG11202010857T A SG 11202010857TA
Authority
SG
Singapore
Prior art keywords
rejuvenated
preparation
application
fibroblast
regenerative
Prior art date
Application number
SG11202010857TA
Other languages
English (en)
Inventor
Min Hu
Yanjiao Li
Junyuan Hu
Original Assignee
Shenzhen Alpha Biopharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shenzhen Alpha Biopharmaceutical Co Ltd filed Critical Shenzhen Alpha Biopharmaceutical Co Ltd
Publication of SG11202010857TA publication Critical patent/SG11202010857TA/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/33—Fibroblasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
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SG11202010857TA 2018-05-01 2019-05-01 Preparation method for rejuvenated regenerative fibroblast and application thereof SG11202010857TA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201810407290.XA CN110423721B (zh) 2018-05-01 2018-05-01 一种年轻化的修复型成纤维细胞的制备方法及其应用
PCT/CN2019/085401 WO2019210870A1 (fr) 2018-05-01 2019-05-01 Procédé de préparation de fibroblaste régénérateur régénéré et son application

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SG11202010857TA true SG11202010857TA (en) 2021-02-25

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Country Link
US (1) US20210213069A1 (fr)
EP (1) EP3789484A4 (fr)
JP (1) JP2021523734A (fr)
CN (1) CN110423721B (fr)
AU (1) AU2019263866B2 (fr)
CA (1) CA3098938C (fr)
SG (1) SG11202010857TA (fr)
WO (1) WO2019210870A1 (fr)

Families Citing this family (14)

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Publication number Priority date Publication date Assignee Title
CN117887659A (zh) * 2018-05-01 2024-04-16 云南济慈再生医学研究院有限公司 一种促进靶细胞向神经细胞分化的方法
CN110423721B (zh) * 2018-05-01 2024-02-27 云南济慈再生医学研究院有限公司 一种年轻化的修复型成纤维细胞的制备方法及其应用
US20210230551A1 (en) * 2018-05-04 2021-07-29 Spinalcyte, Llc Enhancement of fibroblast plasticity for treatment of disc degeneration
CN111166769B (zh) * 2020-03-18 2022-01-11 山东大学 真皮成纤维细胞在治疗黑色素瘤中的应用
CN112143749B (zh) * 2020-08-27 2023-05-12 北京宝易生物技术有限公司 一种长效重组犬干扰素制品及制备方法和应用
CN112430626A (zh) * 2020-11-27 2021-03-02 成都康景生物科技有限公司 一种基因修饰的脐间充质干细胞、制备方法及应用
CN112725437B (zh) * 2021-02-02 2022-12-06 暨南大学 节律基因蛋白表达和rna甲基化修饰在制备衰老检测试剂盒中的应用
CN113278619B (zh) * 2021-07-19 2021-10-15 广东省农业科学院动物科学研究所 双sgRNA、基因敲除载体、基因敲除STING基因的猪成纤维细胞系及其构建方法
CN113893337A (zh) * 2021-08-31 2022-01-07 中山大学 Dtx2蛋白在制备调控端粒酶活性的制剂中的应用
CN115975944A (zh) * 2021-10-14 2023-04-18 北京干细胞与再生医学研究院 年轻化非多能性细胞及其制备方法和应用
CN114381461B (zh) * 2022-03-24 2022-07-19 中国人民解放军军事科学院军事医学研究院 一种通过清除野生型p53蛋白促进ATG5-ATG12共轭增强细胞自噬的应用
WO2023200223A1 (fr) * 2022-04-15 2023-10-19 주식회사 로노 Composition de rajeunissement de cellules sénescentes ou de retardement ou de prévention du vieillissement cellulaire
CN116640727B (zh) * 2023-07-26 2023-09-22 成都高新绮澳医疗美容诊所有限公司 一种提高细胞活力的营养液及其制备方法、应用
CN117159588B (zh) * 2023-11-01 2024-02-13 北京国卫生物科技有限公司 脐带间充质干细胞治疗骨关节炎的细胞治疗方法

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110105393A1 (en) * 2007-08-16 2011-05-05 Andreas Androutsellis-Theotokis Methods for promoting stem cell proliferation and survival
WO2009042798A1 (fr) * 2007-09-26 2009-04-02 Cold Spring Harbor Laboratory Procédés de traitement d'une fibrose par modulation de la sénescence cellulaire
US10035834B2 (en) * 2008-06-18 2018-07-31 The Texas A&M University System Mesenchymal stem cells, compositions, and methods for treatment of cardiac tissue damage
WO2009155583A1 (fr) * 2008-06-19 2009-12-23 The Trustees Of The University Of Pennsylvania Biomatériaux pour le remplacement de tissus
WO2010065567A2 (fr) * 2008-12-01 2010-06-10 Lifespan Extension Llc Procédés et compositions pour modifier la santé, le bien-être et l'espérance de vie
JP2012143158A (ja) * 2009-05-12 2012-08-02 Hiroshima Univ 誘導性間葉系幹細胞およびその作製方法
WO2011159726A2 (fr) * 2010-06-14 2011-12-22 The Scripps Research Institute Reprogrammation de cellules pour leur conférer un nouveau destin
WO2012006577A2 (fr) * 2010-07-08 2012-01-12 Duke University Reprogrammation directe de cellules en un devenir de cardiomyocytes
KR101737164B1 (ko) * 2010-11-12 2017-05-17 알러간, 인코포레이티드 물질대사된 조건화 성장 배지 및 이용 방법
WO2014013014A1 (fr) * 2012-07-18 2014-01-23 Fundació Privada Centre De Regulació Genòmica (Crg) Inhibiteurs de jak pour l'activation de populations de cellules souches épidermiques
WO2014089121A2 (fr) * 2012-12-03 2014-06-12 Thomas Ichim Administration rétrograde de cellules et d'acides nucléiques pour le traitement de maladies cardiovasculaires
WO2015056804A1 (fr) * 2013-10-17 2015-04-23 国立大学法人京都大学 Procédé efficace pour l'établissement de cellules souches pluripotentes induites
WO2016027099A2 (fr) * 2014-08-22 2016-02-25 Cambridge Enterprise Limited Réinitialisation de cellules souches pluripotentes
CN105441384B (zh) * 2014-09-26 2021-01-05 北京大学 一种制备动物和人类原始多潜能干细胞的方法，试剂盒及用途
CN106337037A (zh) * 2015-07-08 2017-01-18 中国科学院上海生命科学研究院 一种诱导成纤维细胞直接向神经细胞转化的药物组合物及其用途
CN105169487A (zh) * 2015-10-27 2015-12-23 上海交通大学 一种具有生物活性的创面修复细胞膜片、制备方法及应用
US20180360924A1 (en) * 2015-11-25 2018-12-20 The Methodist Hospital System Telomere extension and anti-inflammatory agents for cell regeneration
WO2017091943A1 (fr) * 2015-11-30 2017-06-08 Hong Guan Ltd. Procédés améliorés de reprogrammation de cellules non-pluripotentes en cellules souches pluripotentes
US20200080050A1 (en) * 2016-07-11 2020-03-12 Yaakov Nahmias Systems and methods for growing cells in vitro
US20180055887A1 (en) * 2016-08-23 2018-03-01 Academia Sinica Method for preparing induced mesenchymal stem cells and improving mesenchymal stem cell's characters and its applications
CN108060120B (zh) * 2016-11-07 2022-11-04 云南济慈再生医学研究院有限公司 用于分化的细胞重编程的小分子化合物组合、试剂盒及应用
CN108060119B (zh) * 2016-11-07 2021-07-09 云南济慈再生医学研究院有限公司 小分子化合物组合及利用该小分子化合物组合诱导分化的细胞制备血管平滑肌细胞的方法
CN108060123B (zh) * 2016-11-07 2021-07-09 云南济慈再生医学研究院有限公司 一种用于体细胞去分化的靶点调控系统、试剂盒及应用
CN108060122B (zh) * 2016-11-07 2021-07-09 深圳臻德济慈药品研发有限公司 小分子化合物组合及利用该小分子化合物组合诱导分化的细胞制备软骨细胞的方法
CN108060126B (zh) * 2016-11-07 2020-09-08 云南济慈再生医学研究院有限公司 一种诱导分化的细胞制备间充质干细胞的方法及调控靶点的组合
CN108300686B (zh) * 2017-01-13 2022-08-26 云南美颜美龄生物科技有限公司 用于预防、延缓或逆转细胞、组织、器官、机体衰老的小分子化合物/组合，产品及其用途
CN110218696A (zh) * 2018-03-01 2019-09-10 中国科学院广州生物医药与健康研究院 一种用于化学诱导多能性干细胞生成的培养体系以及使用该培养体系的化学重编程方法
CN112567020A (zh) * 2018-03-06 2021-03-26 埃皮博恩股份有限公司 可注射的即用的软骨、肌腱和韧带修复组合物及其使用方法
JP7419347B2 (ja) * 2018-04-23 2024-01-22 エイジェックス・セラピューティクス・インコーポレイテッド 哺乳動物細胞における組織再生の誘導及びセノリシスのための改良された方法
CN110423721B (zh) * 2018-05-01 2024-02-27 云南济慈再生医学研究院有限公司 一种年轻化的修复型成纤维细胞的制备方法及其应用
CN117887659A (zh) * 2018-05-01 2024-04-16 云南济慈再生医学研究院有限公司 一种促进靶细胞向神经细胞分化的方法
WO2019213796A1 (fr) * 2018-05-05 2019-11-14 The Regents Of The University Of California Cellules souches mésenchymateuses régénérées induites et leurs procédés d'utilisation
CN110408594A (zh) * 2019-07-31 2019-11-05 吉林大学 一种将人成纤维细胞高效大量重编程为成熟神经元的方法
CN113025562B (zh) * 2021-03-18 2022-11-29 浙江大学 R406在促进体细胞重编程中的应用、其重编程培养基及方法
US20220296645A1 (en) * 2021-03-22 2022-09-22 Spiritus Therapeutics, Inc. Diagnostic and therapeutic uses of compositions comprising purified, enriched potent exosomes containing disease-based and therapy based signature cargo

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AU2019263866B2 (en) 2023-03-30
EP3789484A4 (fr) 2022-01-05
CA3098938C (fr) 2023-12-05
CN110423721A (zh) 2019-11-08
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US20210213069A1 (en) 2021-07-15
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