SG11202010855XA - Technique for regulating jak-stat pathway to allow cell differentiation, dedifferentiation, and rejuvenation, and applications of technique - Google Patents

Technique for regulating jak-stat pathway to allow cell differentiation, dedifferentiation, and rejuvenation, and applications of technique

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Publication number
SG11202010855XA
SG11202010855XA SG11202010855XA SG11202010855XA SG11202010855XA SG 11202010855X A SG11202010855X A SG 11202010855XA SG 11202010855X A SG11202010855X A SG 11202010855XA SG 11202010855X A SG11202010855X A SG 11202010855XA SG 11202010855X A SG11202010855X A SG 11202010855XA
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Singapore
Prior art keywords
technique
dedifferentiation
rejuvenation
applications
cell differentiation
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SG11202010855XA
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English (en)
Inventor
Min Hu
Yanjiao Li
Junyuan Hu
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Shenzhen Alpha Biopharmaceutical Co Ltd
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Publication date
Application filed by Shenzhen Alpha Biopharmaceutical Co Ltd filed Critical Shenzhen Alpha Biopharmaceutical Co Ltd
Publication of SG11202010855XA publication Critical patent/SG11202010855XA/en

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Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110423719B (zh) * 2018-05-01 2024-02-27 云南济慈再生医学研究院有限公司 调控Jak-Stat通路使细胞分化、去分化、年轻化的技术及其应用
CN110423721B (zh) * 2018-05-01 2024-02-27 云南济慈再生医学研究院有限公司 一种年轻化的修复型成纤维细胞的制备方法及其应用
CN111041027B (zh) * 2019-12-19 2022-10-28 广东省农业科学院动物卫生研究所 一种Atg12基因敲除细胞系构建方法及其应用
CN111269887B (zh) * 2020-02-28 2021-11-16 上海市东方医院(同济大学附属东方医院) 一种人多能干细胞向外胚层分化的方法
CN111778289A (zh) * 2020-07-27 2020-10-16 扬州大学 一种利用CRISPR-Cas9靶向敲除鸡Bmp4基因的方法
CN111944810B (zh) * 2020-07-29 2022-05-27 中国农业大学 靶向敲除TNFα基因的sgRNA和敲除TNFα基因的猪胚胎成纤维细胞系及其应用
CN112022848B (zh) * 2020-08-13 2022-02-22 中南大学湘雅二医院 索拉非尼在治疗1型糖尿病中的应用
CN112662621B (zh) * 2020-12-31 2022-12-27 中国人民解放军总医院 一种逆转间充质干细胞衰老的方法及应用
CN112626010B (zh) * 2021-01-12 2022-05-17 北京大学口腔医学院 Cdc20在间充质干细胞成骨向分化中的应用
WO2023061484A1 (zh) * 2021-10-14 2023-04-20 北京干细胞与再生医学研究院 年轻化非多能性细胞及其制备方法和应用
CN114196676B (zh) * 2021-11-29 2023-07-18 中国农业科学院北京畜牧兽医研究所 Itga2基因在调控猪米色脂肪形成中的应用
CN114958918B (zh) * 2022-06-24 2023-08-25 中国农业科学院哈尔滨兽医研究所(中国动物卫生与流行病学中心哈尔滨分中心) 猪nlrp6基因启动子区的双荧光素酶报告基因载体

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002501738A (ja) * 1998-01-30 2002-01-22 ヒューマン ジノーム サイエンシーズ, インコーポレイテッド 67個のヒト分泌タンパク質
EP1560916A1 (en) * 2002-11-08 2005-08-10 ReproCELL Inc. Expansion agents for stem cells
CN1769447A (zh) * 2004-11-05 2006-05-10 重庆医科大学 抑制慢性粒细胞白血病中stat5活性的诱骗寡核苷酸及其应用
EP1693466A1 (en) * 2005-02-16 2006-08-23 Eppendorf Array Technologies SA Method and kit to profile tumors by biomarker analyses including transcriptional factor assays
US8247227B2 (en) * 2006-08-28 2012-08-21 The Cleveland Clinic Foundation Dendritic cell precursors
WO2008110491A2 (en) * 2007-03-09 2008-09-18 University Of Basel Chemotherapy of neoplastic diseases using combinations of rapamycin and compounds modulating mtor pathway alone or in combination with heat
JP2008261764A (ja) * 2007-04-13 2008-10-30 Kyowa Hakko Kogyo Co Ltd キナーゼ阻害物質の測定方法
EP2179033A1 (en) * 2007-08-16 2010-04-28 The Government of the United States of America as represented by the Secretary of the Department of Health and Human Services Methods for promoting stem cell proliferation and survival
WO2010069008A1 (en) * 2008-12-19 2010-06-24 Griffith University A germline competent cell derived from adult tissue
US9376664B2 (en) * 2010-06-14 2016-06-28 The Scripps Research Institute Reprogramming of cells to a new fate
WO2014013014A1 (en) * 2012-07-18 2014-01-23 Fundació Privada Centre De Regulació Genòmica (Crg) Jak inhibitors for activation of epidermal stem cell populations
WO2015056804A1 (ja) * 2013-10-17 2015-04-23 国立大学法人京都大学 効率的な人工多能性幹細胞の樹立方法
US9618513B2 (en) * 2014-01-27 2017-04-11 The Regents Of The University Of Michigan Compositions and methods for detecting and treating colorectal cancer
EP3183336B1 (en) * 2014-08-22 2020-11-25 Cambridge Enterprise Limited Resetting pluripotent stem cells
KR20180002838A (ko) * 2015-05-07 2018-01-08 더 트러스티스 오브 콜롬비아 유니버시티 인 더 시티 오브 뉴욕 모발 생장을 촉진하기 위한 방법 및 조성물
US20180360924A1 (en) * 2015-11-25 2018-12-20 The Methodist Hospital System Telomere extension and anti-inflammatory agents for cell regeneration
CN109641893B (zh) * 2016-06-20 2021-07-20 大邱庆北尖端医疗产业振兴财团 新的咪唑并吡啶衍生物及其制备方法和用途
US20180055887A1 (en) * 2016-08-23 2018-03-01 Academia Sinica Method for preparing induced mesenchymal stem cells and improving mesenchymal stem cell's characters and its applications
CN113265375B (zh) * 2016-11-07 2022-05-13 云南济慈再生医学研究院有限公司 一种提高细胞活性的方法
CN108060121B (zh) * 2016-11-07 2021-05-28 惠州市正多实业有限公司 小分子化合物组合及利用该小分子化合物组合诱导分化的细胞制备成骨细胞的方法
CN108070549A (zh) * 2016-11-07 2018-05-25 昆明学院 小分子化合物组合及利用该小分子化合物组合诱导分化的细胞制备血管内皮细胞的方法
CN108060126B (zh) * 2016-11-07 2020-09-08 云南济慈再生医学研究院有限公司 一种诱导分化的细胞制备间充质干细胞的方法及调控靶点的组合
CN108060119B (zh) * 2016-11-07 2021-07-09 云南济慈再生医学研究院有限公司 小分子化合物组合及利用该小分子化合物组合诱导分化的细胞制备血管平滑肌细胞的方法
CN108060120B (zh) * 2016-11-07 2022-11-04 云南济慈再生医学研究院有限公司 用于分化的细胞重编程的小分子化合物组合、试剂盒及应用
CN108300686B (zh) * 2017-01-13 2022-08-26 云南美颜美龄生物科技有限公司 用于预防、延缓或逆转细胞、组织、器官、机体衰老的小分子化合物/组合,产品及其用途
CN110423721B (zh) * 2018-05-01 2024-02-27 云南济慈再生医学研究院有限公司 一种年轻化的修复型成纤维细胞的制备方法及其应用
CN110423719B (zh) * 2018-05-01 2024-02-27 云南济慈再生医学研究院有限公司 调控Jak-Stat通路使细胞分化、去分化、年轻化的技术及其应用
WO2019213796A1 (en) * 2018-05-05 2019-11-14 The Regents Of The University Of California INDUCED REJUVENATED MESENCHYMAL STEM CELLS (irMSCs) AND USES THEREOF
TW202302846A (zh) * 2021-04-16 2023-01-16 中國大陸商杭州啟函生物科技有限公司 用於細胞工程化的安全港基因座

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