SG11201911796QA - Mutant strain clostridium thermocellum for producing cellulase and xylanase and preparation method thereof - Google Patents

Mutant strain clostridium thermocellum for producing cellulase and xylanase and preparation method thereof

Info

Publication number
SG11201911796QA
SG11201911796QA SG11201911796QA SG11201911796QA SG11201911796QA SG 11201911796Q A SG11201911796Q A SG 11201911796QA SG 11201911796Q A SG11201911796Q A SG 11201911796QA SG 11201911796Q A SG11201911796Q A SG 11201911796QA SG 11201911796Q A SG11201911796Q A SG 11201911796QA
Authority
SG
Singapore
Prior art keywords
xylanase
preparation
mutant strain
clostridium thermocellum
producing cellulase
Prior art date
Application number
SG11201911796QA
Other languages
English (en)
Inventor
Chakrit Tachaapaikoon
Khanok Ratanakhanokchai
Patthra Pason
Rattiya Waeonukul
Prattana Ketbot
Wikanda Techanan
Kraileark Kittisuriyanont
Narong Kaewsuwan
Original Assignee
Ptt Global Chemical Public Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from TH1701003165A external-priority patent/TH1701003165A/th
Application filed by Ptt Global Chemical Public Co Ltd filed Critical Ptt Global Chemical Public Co Ltd
Publication of SG11201911796QA publication Critical patent/SG11201911796QA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/145—Clostridium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
SG11201911796QA 2017-06-07 2018-05-30 Mutant strain clostridium thermocellum for producing cellulase and xylanase and preparation method thereof SG11201911796QA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
TH1701003165A TH1701003165A (th) 2017-06-07 "แบคทีเรียดัดแปลงพันธุกรรมสายพันธุ์ (mutant strain) คลอสทริเดียม เทอร์โมเซลลัม (Clostridium thermocellum) สำหรับผลิตเอนไซม์เซลลูเลส และไซลาเนส และวิธีผลิต เอนไซม์ดังกล่าว"
PCT/TH2018/000028 WO2018226170A2 (en) 2017-06-07 2018-05-30 Mutant strain clostridium thermocellum for producing cellulase and xylanase and preparation method thereof

Publications (1)

Publication Number Publication Date
SG11201911796QA true SG11201911796QA (en) 2020-01-30

Family

ID=64567084

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201911796QA SG11201911796QA (en) 2017-06-07 2018-05-30 Mutant strain clostridium thermocellum for producing cellulase and xylanase and preparation method thereof

Country Status (6)

Country Link
US (1) US11236375B2 (zh)
JP (1) JP7019926B2 (zh)
CN (1) CN111386341B (zh)
DE (1) DE112018002933B4 (zh)
SG (1) SG11201911796QA (zh)
WO (1) WO2018226170A2 (zh)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111386341B (zh) * 2017-06-07 2023-09-15 Ptt全球化学公共有限公司 用于生产纤维素酶和木聚糖酶的突变株热纤梭菌及其制备方法
CN110540982B (zh) * 2019-09-30 2021-11-02 江南大学 一种提高梭热杆菌纤维素酶酶活的发酵方法
CN111848757B (zh) * 2020-07-27 2022-03-04 齐鲁工业大学 一种适用于低钙离子浓度的纤维小体对接蛋白组合突变体36862及应用
CN114807067A (zh) * 2022-03-31 2022-07-29 苏州聚维元创生物科技有限公司 一种可以分泌漆酶的工程菌株及其构建方法与应用
CN114854655B (zh) * 2022-04-22 2023-10-24 苏州聚维元创生物科技有限公司 一种可以高效分泌木聚糖酶的工程菌株、构建方法与应用
CN116200286B (zh) * 2022-07-07 2024-02-27 哈尔滨工业大学 一株高效糖化纤维素的热纤梭菌及其应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6229974A (ja) * 1985-07-30 1987-02-07 Kubota Ltd キシラナ−ゼ及びその製造法
JP5083735B2 (ja) * 2008-08-30 2012-11-28 独立行政法人国際農林水産業研究センター クロストリジウム属菌並びにセルロソームを含むセルラーゼ及びヘミセルラーゼの製造方法
JP5881234B2 (ja) 2010-07-14 2016-03-09 東レ株式会社 変異型βグルコシダーゼ、バイオマス分解用酵素組成物および糖液の製造方法
US9546385B2 (en) * 2010-12-22 2017-01-17 Enchi Corporation Genetically modified clostridium thermocellum engineered to ferment xylose
BRPI1102151A2 (pt) * 2011-05-11 2014-03-04 Cetrel S A Processo e sistema de produção de biogás a partir da digestão anaeróbia de biomassa vegetal em fase sólida
DK2782998T3 (en) * 2011-11-22 2018-04-16 Novozymes Inc POLYPEPTIDES WITH BETA-XYLOSIDASE ACTIVITY AND POLYNUCLEOTIDES CODING THEM
WO2014007189A1 (ja) 2012-07-03 2014-01-09 東レ株式会社 糖液の製造方法
JP2017035001A (ja) 2013-11-19 2017-02-16 花王株式会社 耐熱性キシラナーゼ
JP2016208852A (ja) * 2015-04-30 2016-12-15 味の素株式会社 脂肪酸を生成する緑藻改変株
CN111386341B (zh) * 2017-06-07 2023-09-15 Ptt全球化学公共有限公司 用于生产纤维素酶和木聚糖酶的突变株热纤梭菌及其制备方法

Also Published As

Publication number Publication date
US11236375B2 (en) 2022-02-01
CN111386341A (zh) 2020-07-07
DE112018002933T5 (de) 2020-02-20
US20210079434A1 (en) 2021-03-18
DE112018002933B4 (de) 2020-11-05
JP2020523019A (ja) 2020-08-06
WO2018226170A3 (en) 2019-03-07
WO2018226170A2 (en) 2018-12-13
CN111386341B (zh) 2023-09-15
JP7019926B2 (ja) 2022-02-16

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