SG11201911796QA - Mutant strain clostridium thermocellum for producing cellulase and xylanase and preparation method thereof - Google Patents

Mutant strain clostridium thermocellum for producing cellulase and xylanase and preparation method thereof

Info

Publication number
SG11201911796QA
SG11201911796QA SG11201911796QA SG11201911796QA SG11201911796QA SG 11201911796Q A SG11201911796Q A SG 11201911796QA SG 11201911796Q A SG11201911796Q A SG 11201911796QA SG 11201911796Q A SG11201911796Q A SG 11201911796QA SG 11201911796Q A SG11201911796Q A SG 11201911796QA
Authority
SG
Singapore
Prior art keywords
xylanase
preparation
mutant strain
clostridium thermocellum
producing cellulase
Prior art date
Application number
SG11201911796QA
Inventor
Chakrit Tachaapaikoon
Khanok Ratanakhanokchai
Patthra Pason
Rattiya Waeonukul
Prattana Ketbot
Wikanda Techanan
Kraileark Kittisuriyanont
Narong Kaewsuwan
Original Assignee
Ptt Global Chemical Public Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from TH1701003165A external-priority patent/TH1701003165A/en
Application filed by Ptt Global Chemical Public Co Ltd filed Critical Ptt Global Chemical Public Co Ltd
Publication of SG11201911796QA publication Critical patent/SG11201911796QA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/145—Clostridium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
SG11201911796QA 2017-06-07 2018-05-30 Mutant strain clostridium thermocellum for producing cellulase and xylanase and preparation method thereof SG11201911796QA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
TH1701003165A TH1701003165A (en) 2017-06-07 "The mutant strain) bacteria, Clostridium thermocellum, for the production of cellulase and xylanase enzymes and the production method of the enzyme.
PCT/TH2018/000028 WO2018226170A2 (en) 2017-06-07 2018-05-30 Mutant strain clostridium thermocellum for producing cellulase and xylanase and preparation method thereof

Publications (1)

Publication Number Publication Date
SG11201911796QA true SG11201911796QA (en) 2020-01-30

Family

ID=64567084

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201911796QA SG11201911796QA (en) 2017-06-07 2018-05-30 Mutant strain clostridium thermocellum for producing cellulase and xylanase and preparation method thereof

Country Status (6)

Country Link
US (1) US11236375B2 (en)
JP (1) JP7019926B2 (en)
CN (1) CN111386341B (en)
DE (1) DE112018002933B4 (en)
SG (1) SG11201911796QA (en)
WO (1) WO2018226170A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111386341B (en) * 2017-06-07 2023-09-15 Ptt全球化学公共有限公司 Mutant clostridium thermocellum for producing cellulase and xylanase and preparation method thereof
CN110540982B (en) * 2019-09-30 2021-11-02 江南大学 Fermentation method for improving activity of Thermobacteroid cellulase
CN111848757B (en) * 2020-07-27 2022-03-04 齐鲁工业大学 Cellulosome docking protein combined mutant 36862 suitable for low calcium ion concentration and application
CN114807067A (en) * 2022-03-31 2022-07-29 苏州聚维元创生物科技有限公司 Engineering strain capable of secreting laccase and construction method and application thereof
CN114854655B (en) * 2022-04-22 2023-10-24 苏州聚维元创生物科技有限公司 Engineering strain capable of efficiently secreting xylanase, construction method and application
CN116200286B (en) * 2022-07-07 2024-02-27 哈尔滨工业大学 Clostridium thermocellum capable of efficiently saccharifying cellulose and application thereof

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6229974A (en) * 1985-07-30 1987-02-07 Kubota Ltd Xylanase and production thereof
JP5083735B2 (en) * 2008-08-30 2012-11-28 独立行政法人国際農林水産業研究センター Clostridial genus, cellulase-containing cellulase and method for producing hemicellulase
JP5881234B2 (en) 2010-07-14 2016-03-09 東レ株式会社 Mutant β-glucosidase, enzyme composition for biomass degradation, and method for producing sugar solution
CN103429751A (en) * 2010-12-22 2013-12-04 马斯科马公司 Genetically modified clostridium thermocellum engineered to ferment xylose
BRPI1102151A2 (en) * 2011-05-11 2014-03-04 Cetrel S A BIOGAS PRODUCTION PROCESS AND SYSTEM FROM SOLID PHASE ANAEROBIC DIGESTION
WO2013075644A1 (en) * 2011-11-22 2013-05-30 Novozymes, Inc. Polypeptides having beta-xylosidase activity and polynucleotides encoding same
US9909158B2 (en) 2012-07-03 2018-03-06 Toray Industries, Inc. Method of producing sugar solution including washing with aqueous alkaline and inorganic salt solutions
JP2017035001A (en) 2013-11-19 2017-02-16 花王株式会社 Heat resistant xylanase
JP2016208852A (en) * 2015-04-30 2016-12-15 味の素株式会社 Green algae modified strain producing fatty acid
CN111386341B (en) 2017-06-07 2023-09-15 Ptt全球化学公共有限公司 Mutant clostridium thermocellum for producing cellulase and xylanase and preparation method thereof

Also Published As

Publication number Publication date
US20210079434A1 (en) 2021-03-18
DE112018002933T5 (en) 2020-02-20
CN111386341B (en) 2023-09-15
DE112018002933B4 (en) 2020-11-05
WO2018226170A3 (en) 2019-03-07
CN111386341A (en) 2020-07-07
JP7019926B2 (en) 2022-02-16
US11236375B2 (en) 2022-02-01
WO2018226170A2 (en) 2018-12-13
JP2020523019A (en) 2020-08-06

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