SG11201900038QA - Compositions and methods comprising improvements of crispr guide rnas using the h1 promoter - Google Patents

Compositions and methods comprising improvements of crispr guide rnas using the h1 promoter

Info

Publication number
SG11201900038QA
SG11201900038QA SG11201900038QA SG11201900038QA SG11201900038QA SG 11201900038Q A SG11201900038Q A SG 11201900038QA SG 11201900038Q A SG11201900038Q A SG 11201900038QA SG 11201900038Q A SG11201900038Q A SG 11201900038QA SG 11201900038Q A SG11201900038Q A SG 11201900038QA
Authority
SG
Singapore
Prior art keywords
international
promoter
crispr
baltimore
compositions
Prior art date
Application number
SG11201900038QA
Other languages
English (en)
Inventor
Vinod Jaskula-Ranga
Donald Zack
Derek Welsbie
Original Assignee
Univ Johns Hopkins
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Johns Hopkins filed Critical Univ Johns Hopkins
Publication of SG11201900038QA publication Critical patent/SG11201900038QA/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0014—Skin, i.e. galenical aspects of topical compositions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C12N2330/51—Specially adapted vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10344—Chimeric viral vector comprising heterologous viral elements for production of another viral vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
SG11201900038QA 2016-07-05 2017-07-05 Compositions and methods comprising improvements of crispr guide rnas using the h1 promoter SG11201900038QA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662358335P 2016-07-05 2016-07-05
PCT/US2017/040707 WO2018009534A1 (en) 2016-07-05 2017-07-05 Compositions and methods comprising improvements of crispr guide rnas using the h1 promoter

Publications (1)

Publication Number Publication Date
SG11201900038QA true SG11201900038QA (en) 2019-02-27

Family

ID=60912275

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201900038QA SG11201900038QA (en) 2016-07-05 2017-07-05 Compositions and methods comprising improvements of crispr guide rnas using the h1 promoter

Country Status (14)

Country Link
US (1) US11766488B2 (zh)
EP (1) EP3481956A4 (zh)
JP (2) JP2019520078A (zh)
KR (1) KR20190039702A (zh)
CN (1) CN109844116A (zh)
AU (2) AU2017292667A1 (zh)
BR (1) BR112019000055A2 (zh)
CA (1) CA3029860A1 (zh)
CL (1) CL2019000025A1 (zh)
EA (1) EA201990213A1 (zh)
IL (1) IL264020A (zh)
MX (1) MX2019000251A (zh)
SG (1) SG11201900038QA (zh)
WO (1) WO2018009534A1 (zh)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11788088B2 (en) * 2017-09-26 2023-10-17 The Board Of Trustees Of The University Of Illinois CRISPR/Cas system and method for genome editing and modulating transcription
MA50877A (fr) 2017-11-21 2020-09-30 Bayer Healthcare Llc Matériaux et méthodes pour le traitement de la rétinite pigmentaire autosomique dominante
CN108728441B (zh) * 2018-04-18 2022-07-22 深圳市第二人民医院 特异性识别p53突变的基因系统
GB2589246A (en) 2018-05-16 2021-05-26 Synthego Corp Methods and systems for guide RNA design and use
WO2019225638A1 (ja) * 2018-05-25 2019-11-28 国立大学法人筑波大学 融合タンパク質、核酸、細胞及び動物の製造方法
JPWO2020059708A1 (ja) * 2018-09-17 2021-09-02 国立大学法人 東京医科歯科大学 Ｃａｓタンパク質の活性調節法
WO2020081438A1 (en) 2018-10-16 2020-04-23 Blueallele, Llc Methods for targeted insertion of dna in genes
US20200140893A1 (en) * 2018-11-01 2020-05-07 Blueallele, Llc Methods for altering gene expression for genetic disorders
JPWO2020096049A1 (ja) * 2018-11-08 2021-11-25 国立大学法人東海国立大学機構 単一のａａｖベクターによるゲノム編集を用いた遺伝子治療
WO2020264254A1 (en) * 2019-06-28 2020-12-30 Crispr Therapeutics Ag Materials and methods for controlling gene editing
AU2020310837A1 (en) * 2019-07-08 2022-02-24 Inscripta, Inc. Increased nucleic acid-guided cell editing via a LexA-Rad51 fusion protein
CN110622921B (zh) * 2019-09-29 2021-06-15 江苏大学 阿尔茨海默病病变区FoxG1过表达小鼠模型的构建方法及应用
CN110607302A (zh) * 2019-10-09 2019-12-24 广州医科大学附属第二医院 检测花生四烯酸脂氧合酶aloxe3基因启动子突变的方法及其应用
CN111718933B (zh) * 2020-06-28 2022-01-28 暨南大学 一种rrbp1基因敲除热带爪蛙模型的制备方法与应用
WO2022212768A2 (en) * 2021-03-31 2022-10-06 Hunterian Medicine Llc Compact promoters for gene editing
WO2023120536A1 (ja) * 2021-12-21 2023-06-29 国立大学法人東海国立大学機構 単一のａａｖベクターによるゲノム編集を用いた遺伝子治療

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4217344A (en) 1976-06-23 1980-08-12 L'oreal Compositions containing aqueous dispersions of lipid spheres
US4198626A (en) 1977-09-02 1980-04-15 Rauscher Frank J Intravenous alarm device
US4235871A (en) 1978-02-24 1980-11-25 Papahadjopoulos Demetrios P Method of encapsulating biologically active materials in lipid vesicles
US4186183A (en) 1978-03-29 1980-01-29 The United States Of America As Represented By The Secretary Of The Army Liposome carriers in chemotherapy of leishmaniasis
US4261975A (en) 1979-09-19 1981-04-14 Merck & Co., Inc. Viral liposome particle
US4485054A (en) 1982-10-04 1984-11-27 Lipoderm Pharmaceuticals Limited Method of encapsulating biologically active materials in multilamellar lipid vesicles (MLV)
US4501728A (en) 1983-01-06 1985-02-26 Technology Unlimited, Inc. Masking of liposomes from RES recognition
US4946787A (en) 1985-01-07 1990-08-07 Syntex (U.S.A.) Inc. N-(ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US4897355A (en) 1985-01-07 1990-01-30 Syntex (U.S.A.) Inc. N[ω,(ω-1)-dialkyloxy]- and N-[ω,(ω-1)-dialkenyloxy]-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US5049386A (en) 1985-01-07 1991-09-17 Syntex (U.S.A.) Inc. N-ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)Alk-1-YL-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US4797368A (en) 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US4774085A (en) 1985-07-09 1988-09-27 501 Board of Regents, Univ. of Texas Pharmaceutical administration systems containing a mixture of immunomodulators
US4837028A (en) 1986-12-24 1989-06-06 Liposome Technology, Inc. Liposomes with enhanced circulation time
US4873316A (en) 1987-06-23 1989-10-10 Biogen, Inc. Isolation of exogenous recombinant proteins from the milk of transgenic mammals
US4931777A (en) 1988-11-16 1990-06-05 Chiang Cheng San Low level alarm for drop-feed injection liquid
US5264618A (en) 1990-04-19 1993-11-23 Vical, Inc. Cationic lipids for intracellular delivery of biologically active molecules
WO1991017424A1 (en) 1990-05-03 1991-11-14 Vical, Inc. Intracellular delivery of biologically active substances by means of self-assembling lipid complexes
US5173414A (en) 1990-10-30 1992-12-22 Applied Immune Sciences, Inc. Production of recombinant adeno-associated virus vectors
US5112319A (en) 1991-05-14 1992-05-12 Eric Lai Infusion alarm system
US5587308A (en) 1992-06-02 1996-12-24 The United States Of America As Represented By The Department Of Health & Human Services Modified adeno-associated virus vector capable of expression from a novel promoter
US6121555A (en) 1997-10-10 2000-09-19 Northgate Technologies Incorporated Fluid container sensor
US7750817B2 (en) 1999-12-10 2010-07-06 Beverage Metrics Holding Ltd System and method using a scale for monitoring the dispensing of a beverage
JP2001343294A (ja) 2000-05-31 2001-12-14 Ishida Co Ltd ロードセル及び秤
GB0130955D0 (en) * 2001-12-24 2002-02-13 Cancer Res Ventures Expression system
US7180015B2 (en) 2004-09-24 2007-02-20 Hudson Douglas A Scale with clampable protrusion for weighing bicycles and other sports-related items
US10022457B2 (en) * 2005-08-05 2018-07-17 Gholam A. Peyman Methods to regulate polarization and enhance function of cells
US20070106177A1 (en) 2005-10-11 2007-05-10 Yoshito Hama Apparatus for collecting and calculating quantity of patient fluid loss and method of using same
US20080293142A1 (en) * 2007-04-19 2008-11-27 The Board Of Regents For Oklahoma State University Multiple shRNA Expression Vectors and Methods of Construction
KR101196701B1 (ko) 2009-07-16 2012-11-07 주식회사 실티 가축용 자동 급수장치
US8889394B2 (en) 2009-09-07 2014-11-18 Empire Technology Development Llc Multiple domain proteins
WO2012166662A1 (en) 2011-05-27 2012-12-06 Pet Wireless, Llc Systems, methods and computer program products for monitoring the behavior, health, and/or characteristics of an animal
EP3417880A1 (en) * 2013-06-05 2018-12-26 Duke University Rna-guided gene editing and gene regulation
US20150251894A1 (en) 2014-03-06 2015-09-10 Julie Barbara Lake Transportable Device for Automatically Filling Water Buckets, Troughs and Containers
EP3553176A1 (en) * 2014-03-10 2019-10-16 Editas Medicine, Inc. Crispr/cas-related methods and compositions for treating leber's congenital amaurosis 10 (lca10)
AU2015236128A1 (en) 2014-03-25 2016-11-10 Editas Medicine Inc. CRISPR/CAS-related methods and compositions for treating HIV infection and AIDS
CA2952697A1 (en) 2014-06-16 2015-12-23 The Johns Hopkins University Compositions and methods for the expression of crispr guide rnas using the h1 promoter
EP2982758A1 (en) 2014-08-04 2016-02-10 Centre Hospitalier Universitaire Vaudois (CHUV) Genome editing for the treatment of huntington's disease
US20160090603A1 (en) 2014-09-30 2016-03-31 Sandia Corporation Delivery platforms for the domestication of algae and plants
US20180250424A1 (en) * 2014-10-10 2018-09-06 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
EP3215166B1 (en) 2014-10-31 2024-04-24 The Trustees of the University of Pennsylvania Altering gene expression in car-t cells and uses thereof
CA2963820A1 (en) * 2014-11-07 2016-05-12 Editas Medicine, Inc. Methods for improving crispr/cas-mediated genome-editing
CN104531632A (zh) 2014-11-18 2015-04-22 李云英 快速降解的Cas9-ODC422-461融合蛋白及其应用
CA2970370A1 (en) 2014-12-24 2016-06-30 Massachusetts Institute Of Technology Crispr having or associated with destabilization domains
CN204440762U (zh) 2015-02-09 2015-07-01 刘苹芳 阿基米德原理演示仪
US20160376610A1 (en) 2015-06-24 2016-12-29 Sigma-Aldrich Co. Llc Cell cycle dependent genome regulation and modification
GB2541459B (en) 2015-08-21 2021-01-13 Carton Bernard A method and system for monitoring pregnancy toxaemia

Also Published As

Publication number Publication date
EP3481956A4 (en) 2020-07-08
CN109844116A (zh) 2019-06-04
IL264020A (en) 2019-02-03
AU2017292667A1 (en) 2019-02-21
US20190314521A1 (en) 2019-10-17
CA3029860A1 (en) 2018-01-11
CL2019000025A1 (es) 2019-06-21
AU2023266246A1 (en) 2023-12-07
EA201990213A1 (ru) 2019-11-29
KR20190039702A (ko) 2019-04-15
MX2019000251A (es) 2019-10-09
US11766488B2 (en) 2023-09-26
WO2018009534A1 (en) 2018-01-11
JP2019520078A (ja) 2019-07-18
BR112019000055A2 (pt) 2019-04-02
JP2022153576A (ja) 2022-10-12
EP3481956A1 (en) 2019-05-15

Similar Documents

Publication Publication Date Title
SG11201900038QA (en) Compositions and methods comprising improvements of crispr guide rnas using the h1 promoter
SG11201808622SA (en) Chimeric receptors to flt3 and methods of use thereof
SG11201804373VA (en) Compositions and methods for immunooncology
SG11201807912SA (en) Vaccine against rsv
SG11201809912UA (en) Hybrid carriers for nucleic acid cargo
SG11201909203WA (en) Tissue selective transgene expression
SG11201900028VA (en) Crispr/cas9-based compositions and methods for treating cancer
SG11201805162VA (en) Plasmid constructs for heterologous protein expression and methods of use
SG11201805805VA (en) Recombinogenic nucleic acid strands in situ
SG11201909432SA (en) Compounds for increasing genome editing efficiency
SG11201901206SA (en) Trimer stabilizing hiv envelope protein mutations
SG11201811151PA (en) Extracellular vesicle comprising a fusion protein having fc binding capacity
SG11201804934PA (en) Novel Compounds
SG11201805835WA (en) Modified oncolytic virus
SG11201804070XA (en) Viral vectors encoding recombinant fviii variants with increased expression for gene therapy of hemophilia a
SG11201804694SA (en) Dual controls for therapeutic cell activation or elimination
SG11201907889YA (en) Glycan-interacting compounds and methods of use
SG11201803906PA (en) Control of cellular redox levels
SG11201908919XA (en) Combination therapies targeting pd-1, tim-3, and lag-3
SG11201805709RA (en) Anti-pro/latent myostatin antibodies and methods of use thereof
SG11201811150WA (en) Ev-mediated delivery of binding protein-small molecule conjugates
SG11201909011PA (en) Niraparib compositions
SG11201901020RA (en) Anti-siglec-7 antibodies for the treatment of cancer
SG11202000145XA (en) Methods and systems for conditionally regulating gene expression
SG11202000312UA (en) Encapsulated polynucleotides and methods of use