MX2019000251A - Composiciones y metodos que comprenden mejoras de los arn guias de crispr usando el promotor h1. - Google Patents

Composiciones y metodos que comprenden mejoras de los arn guias de crispr usando el promotor h1.

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Publication number
MX2019000251A
MX2019000251A MX2019000251A MX2019000251A MX2019000251A MX 2019000251 A MX2019000251 A MX 2019000251A MX 2019000251 A MX2019000251 A MX 2019000251A MX 2019000251 A MX2019000251 A MX 2019000251A MX 2019000251 A MX2019000251 A MX 2019000251A
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Mexico
Prior art keywords
addition
promoter
crispr
modifications
sequences
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MX2019000251A
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English (en)
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Zack Donald
Jaskula-Ranga Vinod
S Welsbie Derek
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Univ Johns Hopkins
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Publication of MX2019000251A publication Critical patent/MX2019000251A/es

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    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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Abstract

La materia aquí descrita proporciona composiciones y métodos que comprenden mejoras de un sistema CRISPR (por ejemplo, CRISPR-proteína asociada (cas) 9 (sistema CRISPR-cas9, sistemas CRISPR sin cas9). Tales composiciones pueden comprender modificaciones en la región promotora H1, además de modificaciones en UTR 5´, diferentes secuencias ortólogas del promotor H1, nuevas secuencias del promotor bidireccional compacto con actividad tanto Pol II como Pol III, adición de secuencias de consenso de Kozak, secuencias de terminación, adición de la expresión condicional del promotor bidireccional Pol Il/Pol III, adición de una secuencia molde donante para corregir mutaciones, o combinaciones de las mismas. Otros aspectos de la invención se relacionan con modificaciones de cas9 a través de fusiones de regulación postranscripcional del ciclo celular, sitios diana parciales diseñados por ingeniería genética de manera tal que la nucleasa puede unirse a ellos sin escindir el ADN, sitios de autorregulación, y modificaciones n-terminales para modular la semivida.
MX2019000251A 2016-07-05 2017-07-05 Composiciones y metodos que comprenden mejoras de los arn guias de crispr usando el promotor h1. MX2019000251A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662358335P 2016-07-05 2016-07-05
PCT/US2017/040707 WO2018009534A1 (en) 2016-07-05 2017-07-05 Compositions and methods comprising improvements of crispr guide rnas using the h1 promoter

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MX2019000251A true MX2019000251A (es) 2019-10-09

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US (1) US11766488B2 (es)
EP (1) EP3481956A4 (es)
JP (2) JP2019520078A (es)
KR (1) KR20190039702A (es)
CN (1) CN109844116A (es)
AU (2) AU2017292667A1 (es)
BR (1) BR112019000055A2 (es)
CA (1) CA3029860A1 (es)
CL (1) CL2019000025A1 (es)
EA (1) EA201990213A1 (es)
IL (1) IL264020A (es)
MX (1) MX2019000251A (es)
SG (1) SG11201900038QA (es)
WO (1) WO2018009534A1 (es)

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