SG11201704625UA - Novel methods for enzyme mediated polypeptide conjugation using sortase - Google Patents

Novel methods for enzyme mediated polypeptide conjugation using sortase

Info

Publication number
SG11201704625UA
SG11201704625UA SG11201704625UA SG11201704625UA SG11201704625UA SG 11201704625U A SG11201704625U A SG 11201704625UA SG 11201704625U A SG11201704625U A SG 11201704625UA SG 11201704625U A SG11201704625U A SG 11201704625UA SG 11201704625U A SG11201704625U A SG 11201704625UA
Authority
SG
Singapore
Prior art keywords
sortase
novel methods
enzyme mediated
polypeptide conjugation
mediated polypeptide
Prior art date
Application number
SG11201704625UA
Other languages
English (en)
Inventor
Mara Boenitz-Dulat
Peter Kratzsch
Martin Schatte
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of SG11201704625UA publication Critical patent/SG11201704625UA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/2207—Sortase A (3.4.22.70)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
SG11201704625UA 2014-12-17 2015-12-14 Novel methods for enzyme mediated polypeptide conjugation using sortase SG11201704625UA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14198532 2014-12-17
PCT/EP2015/079617 WO2016096741A1 (en) 2014-12-17 2015-12-14 Novel methods for enzyme mediated polypeptide conjugation using sortase

Publications (1)

Publication Number Publication Date
SG11201704625UA true SG11201704625UA (en) 2017-07-28

Family

ID=52338848

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201704625UA SG11201704625UA (en) 2014-12-17 2015-12-14 Novel methods for enzyme mediated polypeptide conjugation using sortase

Country Status (11)

Country Link
US (1) US10190182B2 (ja)
EP (1) EP3233905B1 (ja)
JP (1) JP6618539B2 (ja)
KR (1) KR20170094216A (ja)
CN (1) CN107001447B (ja)
BR (1) BR112017010775A2 (ja)
CA (1) CA2966287A1 (ja)
MX (1) MX2017007503A (ja)
RU (1) RU2714963C2 (ja)
SG (1) SG11201704625UA (ja)
WO (1) WO2016096741A1 (ja)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110295157A (zh) * 2016-08-25 2019-10-01 北京大学 一种金黄色葡萄球菌分选酶a高效突变体
CN116888258A (zh) * 2021-02-04 2023-10-13 西湖生物医药科技(杭州)有限公司 修饰的红细胞及其治疗高尿酸血症和痛风的用途
WO2023134573A1 (en) * 2022-01-12 2023-07-20 Westlake Therapeutics (Shanghai) Co., Limited Modified cells and uses thereof
CN114487409B (zh) * 2022-04-14 2022-07-19 启德医药科技（苏州）有限公司 一种转肽酶活性的检测方法及检测试剂盒
CN116949004B (zh) * 2023-07-13 2024-04-09 苏州源启生物科技有限公司 一种转肽酶Sortase A及其制备方法和应用

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4150149A (en) 1976-11-29 1979-04-17 Professional Staff Association Of The Los Angeles County Harbor General Hospital Method and means for the early detection and diagnosis of certain types of cancers
US4275149A (en) 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
US4318980A (en) 1978-04-10 1982-03-09 Miles Laboratories, Inc. Heterogenous specific binding assay employing a cycling reactant as label
US4361544A (en) 1980-03-03 1982-11-30 Goldenberg Milton David Tumor localization and therapy with labeled antibodies specific to intracellular tumor-associated markers
US4444744A (en) 1980-03-03 1984-04-24 Goldenberg Milton David Tumor localization and therapy with labeled antibodies to cell surface antigens
US5316757A (en) 1984-10-18 1994-05-31 Board Of Regents, The University Of Texas System Synthesis of polyazamacrocycles with more than one type of side-chain chelating groups
US5342606A (en) 1984-10-18 1994-08-30 Board Of Regents, The University Of Texas System Polyazamacrocyclic compounds for complexation of metal ions
US4737456A (en) 1985-05-09 1988-04-12 Syntex (U.S.A.) Inc. Reducing interference in ligand-receptor binding assays
US5959177A (en) 1989-10-27 1999-09-28 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
CA2116774C (en) 1991-09-19 2003-11-11 Paul J. Carter Expression in e. coli antibody fragments having at least a cysteine present as a free thiol. use for the production of bifunctional f(ab') 2 antibodies
US5739294A (en) 1991-12-10 1998-04-14 The Dow Chemical Company Bicyclopol yazamacrocyclophosphonic acid complexes for use as contrast agents
US5428139A (en) 1991-12-10 1995-06-27 The Dow Chemical Company Bicyclopolyazamacrocyclophosphonic acid complexes for use as radiopharmaceuticals
US5480990A (en) 1991-12-10 1996-01-02 The Dow Chemical Company Bicyclopolyazamacrocyclocarboxylic acid complexes for use as contrast agents
ZA932522B (en) 1992-04-10 1993-12-20 Res Dev Foundation Immunotoxins directed against c-erbB-2(HER/neu) related surface antigens
NZ258392A (en) 1992-11-13 1997-09-22 Idec Pharma Corp Chimeric and radiolabelled antibodies to the b lymphocyte cellsurface antigen bp35 (cd-20) and their use in the treatment of b cell lymphona
US5385893A (en) 1993-05-06 1995-01-31 The Dow Chemical Company Tricyclopolyazamacrocyclophosphonic acids, complexes and derivatives thereof, for use as contrast agents
US5462725A (en) 1993-05-06 1995-10-31 The Dow Chemical Company 2-pyridylmethylenepolyazamacrocyclophosphonic acids, complexes and derivatives thereof, for use as contrast agents
US5789199A (en) 1994-11-03 1998-08-04 Genentech, Inc. Process for bacterial production of polypeptides
US5840523A (en) 1995-03-01 1998-11-24 Genetech, Inc. Methods and compositions for secretion of heterologous polypeptides
US5834456A (en) 1996-02-23 1998-11-10 The Dow Chemical Company Polyazamacrocyclofluoromonoalkylphosphonic acids, and their complexes, for use as contrast agents
US6040498A (en) 1998-08-11 2000-03-21 North Caroline State University Genetically engineered duckweed
US6528624B1 (en) 1998-04-02 2003-03-04 Genentech, Inc. Polypeptide variants
US7125978B1 (en) 1999-10-04 2006-10-24 Medicago Inc. Promoter for regulating expression of foreign genes
DE60022369T2 (de) 1999-10-04 2006-05-18 Medicago Inc., Sainte Foy Verfahren zur regulation der transkription von fremden genen in gegenwart von stickstoff
US20100111856A1 (en) 2004-09-23 2010-05-06 Herman Gill Zirconium-radiolabeled, cysteine engineered antibody conjugates
UA95958C2 (en) * 2006-05-30 2011-09-26 Дженентек, Инк. Antibody that binds to cd22, immunoconjugates and uses therefor
US8940501B2 (en) * 2009-01-30 2015-01-27 Whitehead Institute For Biomedical Research Methods for ligation and uses thereof
ES2589769T3 (es) 2009-02-27 2016-11-16 Massachusetts Institute Of Technology Proteínas modificadas con alta afinidad por quelatos de DOTA
CN103764665A (zh) 2011-06-28 2014-04-30 怀特黑德生物医学研究所 使用分选酶安装用于蛋白质连接的点击化学柄
EP2867253B1 (en) 2012-06-27 2016-09-14 F. Hoffmann-La Roche AG Method for selection and production of tailor-made highly selective and multi-specific targeting entities containing at least two different binding entities and uses thereof

Also Published As

Publication number Publication date
EP3233905A1 (en) 2017-10-25
CN107001447A (zh) 2017-08-01
US10190182B2 (en) 2019-01-29
JP6618539B2 (ja) 2019-12-11
CA2966287A1 (en) 2016-06-23
EP3233905B1 (en) 2020-01-29
RU2017122029A (ru) 2019-01-17
CN107001447B (zh) 2021-07-23
WO2016096741A1 (en) 2016-06-23
RU2017122029A3 (ja) 2019-07-17
KR20170094216A (ko) 2017-08-17
JP2018500901A (ja) 2018-01-18
RU2714963C2 (ru) 2020-02-21
US20170292166A1 (en) 2017-10-12
MX2017007503A (es) 2017-10-04
BR112017010775A2 (pt) 2018-02-27

Similar Documents

Publication Publication Date Title
IL251194A0 (en) Fusion proteins, recombinant bacteria, and methods for using recombinant bacteria
IL247096A0 (en) proteins fc multimers
EP3122371A4 (en) Fgf23 fusion proteins
HK1232136A1 (zh) 融合蛋白
IL247246A0 (en) proteins fc multimers
EP3037812C0 (en) ENZYME ELECTRODE
HK1232234A1 (zh) 融合蛋白
EP3149176A4 (en) Methods for altering polypeptide expression
HRP20182029T1 (hr) Uti-fuzijski proteini
EP3164410B8 (en) Protein recovery
SG11201704625UA (en) Novel methods for enzyme mediated polypeptide conjugation using sortase
EP3204427A4 (en) Chimeric proteins
SG11201606501PA (en) Nanobody-fluorescent protein fusion
IL246533A0 (en) new proteins
AU2014904028A0 (en) Chimeric proteins
EP3221449A4 (en) Process for refolding recombinant chymotrypsin
GB201418989D0 (en) Novel conjugation process and reagents
GB201418186D0 (en) Novel conjugation process and reagents
GB201419237D0 (en) Novel polypeptides
GB201412797D0 (en) Proteins
GB201412649D0 (en) Proteins
GB201412646D0 (en) Proteins
GB201412648D0 (en) Proteins
GB201406894D0 (en) Proteins
GB201405955D0 (en) Proteins