RU2017122029A - Новые способы опосредованного ферментами конъюгирования полипептидов с использованием сортазы - Google Patents
Новые способы опосредованного ферментами конъюгирования полипептидов с использованием сортазы Download PDFInfo
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- RU2017122029A RU2017122029A RU2017122029A RU2017122029A RU2017122029A RU 2017122029 A RU2017122029 A RU 2017122029A RU 2017122029 A RU2017122029 A RU 2017122029A RU 2017122029 A RU2017122029 A RU 2017122029A RU 2017122029 A RU2017122029 A RU 2017122029A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/46—Hybrid immunoglobulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6472—Cysteine endopeptidases (3.4.22)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/22—Cysteine endopeptidases (3.4.22)
- C12Y304/2207—Sortase A (3.4.22.70)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/52—Constant or Fc region; Isotype
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Oncology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
Claims (11)
1. Способ получения продукта ферментативного конъюгирования двух полипептидов, включающий следующие стадии:
инкубирование
i) первого полипептида, содержащего аминокислотную последовательность LPXTG (SEQ ID NO: 20, где Х может быть любым аминокислотным остатком),
ii) второго полипептида, который содержит i) аланинильное соединение на его N-конце или ii) олигоаланин Am (m равен 2 (SEQ ID NO: 26) или 3 (SEQ ID NO: 27), или 4 (SEQ ID NO: 28), или 5 (SEQ ID NO: 29)), или iii) аминокислотный остаток цистеина с последующими одним-тремя аминокислотными остатками аланина на его N-конце,
iii) третьего полипептида с сортазной активностью, который получен из сортазы А Staphylococcus aureus, и
выделение конъюгата из реакционной смеси и, посредством этого, получение продукта ферментативного конъюгирования двух полипептидов.
2. Способ по п. 1, в котором первый полипептид содержит на его С-конце аминокислотную последовательность LPXTG (SEQ ID NO: 20, где Х может быть любым аминокислотным остатком).
3. Способ по п. 2, в котором первый полипептид содержит на его С-конце аминокислотную последовательность LPETG (SEQ ID NO: 30).
4. Способ по любому из пп. 1-3, в котором второй полипептид имеет на его N-конце олигоаланин SEQ ID NO: 26 или SEQ ID NO: 27.
5. Способ по любому из пп. 1-4, в котором первый полипептид и второй полипептид независимо друг от друга выбраны из вариабельного домена антитела, фрагмента Fab тяжелой цепи антитела, области Fc антитела, метки, пептида, содержащего аминокислотную последовательность LPXTG (SEQ ID NO: 20, где Х может быть любым аминокислотным остатком), линкера и группировки несортазного мотива.
6. Способ по любому из пп. 1-5, в котором третий полипептид имеет аминокислотную последовательность SEQ ID NO: 21.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP14198532.5 | 2014-12-17 | ||
| EP14198532 | 2014-12-17 | ||
| PCT/EP2015/079617 WO2016096741A1 (en) | 2014-12-17 | 2015-12-14 | Novel methods for enzyme mediated polypeptide conjugation using sortase |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| RU2017122029A true RU2017122029A (ru) | 2019-01-17 |
| RU2017122029A3 RU2017122029A3 (ru) | 2019-07-17 |
| RU2714963C2 RU2714963C2 (ru) | 2020-02-21 |
Family
ID=52338848
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2017122029A RU2714963C2 (ru) | 2014-12-17 | 2015-12-14 | Новые способы опосредованного ферментами конъюгирования полипептидов с использованием сортазы |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US10190182B2 (ru) |
| EP (1) | EP3233905B1 (ru) |
| JP (1) | JP6618539B2 (ru) |
| KR (1) | KR20170094216A (ru) |
| CN (1) | CN107001447B (ru) |
| BR (1) | BR112017010775A2 (ru) |
| CA (1) | CA2966287A1 (ru) |
| MX (1) | MX2017007503A (ru) |
| RU (1) | RU2714963C2 (ru) |
| SG (1) | SG11201704625UA (ru) |
| WO (1) | WO2016096741A1 (ru) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106191015B (zh) * | 2016-08-25 | 2019-09-13 | 北京大学 | 金黄色葡萄球菌分选酶a高效突变体 |
| CN116888258A (zh) * | 2021-02-04 | 2023-10-13 | 西湖生物医药科技(杭州)有限公司 | 修饰的红细胞及其治疗高尿酸血症和痛风的用途 |
| WO2023134573A1 (en) * | 2022-01-12 | 2023-07-20 | Westlake Therapeutics (Shanghai) Co., Limited | Modified cells and uses thereof |
| CN114487409B (zh) * | 2022-04-14 | 2022-07-19 | 启德医药科技（苏州）有限公司 | 一种转肽酶活性的检测方法及检测试剂盒 |
| CN116949004B (zh) * | 2023-07-13 | 2024-04-09 | 苏州源启生物科技有限公司 | 一种转肽酶Sortase A及其制备方法和应用 |
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| US4150149A (en) | 1976-11-29 | 1979-04-17 | Professional Staff Association Of The Los Angeles County Harbor General Hospital | Method and means for the early detection and diagnosis of certain types of cancers |
| US4275149A (en) | 1978-11-24 | 1981-06-23 | Syva Company | Macromolecular environment control in specific receptor assays |
| US4318980A (en) | 1978-04-10 | 1982-03-09 | Miles Laboratories, Inc. | Heterogenous specific binding assay employing a cycling reactant as label |
| US4444744A (en) | 1980-03-03 | 1984-04-24 | Goldenberg Milton David | Tumor localization and therapy with labeled antibodies to cell surface antigens |
| US4361544A (en) | 1980-03-03 | 1982-11-30 | Goldenberg Milton David | Tumor localization and therapy with labeled antibodies specific to intracellular tumor-associated markers |
| US5316757A (en) | 1984-10-18 | 1994-05-31 | Board Of Regents, The University Of Texas System | Synthesis of polyazamacrocycles with more than one type of side-chain chelating groups |
| US5342606A (en) | 1984-10-18 | 1994-08-30 | Board Of Regents, The University Of Texas System | Polyazamacrocyclic compounds for complexation of metal ions |
| US4737456A (en) | 1985-05-09 | 1988-04-12 | Syntex (U.S.A.) Inc. | Reducing interference in ligand-receptor binding assays |
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-
2015
- 2015-12-14 MX MX2017007503A patent/MX2017007503A/es unknown
- 2015-12-14 CN CN201580068354.2A patent/CN107001447B/zh active Active
- 2015-12-14 EP EP15817140.5A patent/EP3233905B1/en active Active
- 2015-12-14 RU RU2017122029A patent/RU2714963C2/ru active
- 2015-12-14 BR BR112017010775A patent/BR112017010775A2/pt active Search and Examination
- 2015-12-14 KR KR1020177016141A patent/KR20170094216A/ko active Search and Examination
- 2015-12-14 JP JP2017531742A patent/JP6618539B2/ja active Active
- 2015-12-14 SG SG11201704625UA patent/SG11201704625UA/en unknown
- 2015-12-14 WO PCT/EP2015/079617 patent/WO2016096741A1/en active Application Filing
- 2015-12-14 CA CA2966287A patent/CA2966287A1/en active Pending
-
2017
- 2017-06-16 US US15/625,975 patent/US10190182B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| RU2017122029A3 (ru) | 2019-07-17 |
| CN107001447B (zh) | 2021-07-23 |
| MX2017007503A (es) | 2017-10-04 |
| SG11201704625UA (en) | 2017-07-28 |
| CN107001447A (zh) | 2017-08-01 |
| JP2018500901A (ja) | 2018-01-18 |
| CA2966287A1 (en) | 2016-06-23 |
| JP6618539B2 (ja) | 2019-12-11 |
| EP3233905B1 (en) | 2020-01-29 |
| BR112017010775A2 (pt) | 2018-02-27 |
| WO2016096741A1 (en) | 2016-06-23 |
| US20170292166A1 (en) | 2017-10-12 |
| KR20170094216A (ko) | 2017-08-17 |
| EP3233905A1 (en) | 2017-10-25 |
| RU2714963C2 (ru) | 2020-02-21 |
| US10190182B2 (en) | 2019-01-29 |
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