SG11201609053YA - Multiplexed single cell gene expression analysis using template switch and tagmentation - Google Patents
Multiplexed single cell gene expression analysis using template switch and tagmentationInfo
- Publication number
- SG11201609053YA SG11201609053YA SG11201609053YA SG11201609053YA SG11201609053YA SG 11201609053Y A SG11201609053Y A SG 11201609053YA SG 11201609053Y A SG11201609053Y A SG 11201609053YA SG 11201609053Y A SG11201609053Y A SG 11201609053YA SG 11201609053Y A SG11201609053Y A SG 11201609053YA
- Authority
- SG
- Singapore
- Prior art keywords
- tagmentation
- gene expression
- single cell
- expression analysis
- cell gene
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461985983P | 2014-04-29 | 2014-04-29 | |
| US201461987433P | 2014-05-01 | 2014-05-01 | |
| PCT/US2015/028062 WO2015168161A2 (en) | 2014-04-29 | 2015-04-28 | Multiplexed single cell gene expression analysis using template switch and tagmentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201609053YA true SG11201609053YA (en) | 2016-11-29 |
Family
ID=53055134
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201609053YA SG11201609053YA (en) | 2014-04-29 | 2015-04-28 | Multiplexed single cell gene expression analysis using template switch and tagmentation |
| SG10201912516SA SG10201912516SA (en) | 2014-04-29 | 2015-04-28 | Multiplexed single cell gene expression analysis using template switch and tagmentation |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10201912516SA SG10201912516SA (en) | 2014-04-29 | 2015-04-28 | Multiplexed single cell gene expression analysis using template switch and tagmentation |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US20170044525A1 (ja) |
| EP (2) | EP3680333A1 (ja) |
| JP (1) | JP6412954B2 (ja) |
| CN (1) | CN106459967A (ja) |
| AU (4) | AU2015253299B2 (ja) |
| CA (2) | CA2947426C (ja) |
| SG (2) | SG11201609053YA (ja) |
| WO (1) | WO2015168161A2 (ja) |
Families Citing this family (95)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US10466160B2 (en) | 2011-08-01 | 2019-11-05 | Celsee Diagnostics, Inc. | System and method for retrieving and analyzing particles |
| US9404864B2 (en) | 2013-03-13 | 2016-08-02 | Denovo Sciences, Inc. | System for imaging captured cells |
| WO2013019491A1 (en) | 2011-08-01 | 2013-02-07 | Denovo Sciences | Cell capture system and method of use |
| JP6375230B2 (ja) | 2012-02-27 | 2018-08-15 | セルラー リサーチ， インコーポレイテッド | 分子計数のための組成物およびキット |
| US9606102B2 (en) | 2013-01-26 | 2017-03-28 | Denovo Sciences, Inc. | System and method for capturing and analyzing cells |
| US9707562B2 (en) | 2013-03-13 | 2017-07-18 | Denovo Sciences, Inc. | System for capturing and analyzing cells |
| US9856535B2 (en) | 2013-05-31 | 2018-01-02 | Denovo Sciences, Inc. | System for isolating cells |
| US10391490B2 (en) | 2013-05-31 | 2019-08-27 | Celsee Diagnostics, Inc. | System and method for isolating and analyzing cells |
| KR20230074639A (ko) | 2013-08-28 | 2023-05-30 | 벡톤 디킨슨 앤드 컴퍼니 | 대량의 동시 단일 세포 분석 |
| US10975371B2 (en) | 2014-04-29 | 2021-04-13 | Illumina, Inc. | Nucleic acid sequence analysis from single cells |
| CN107208158B (zh) | 2015-02-27 | 2022-01-28 | 贝克顿迪金森公司 | 空间上可寻址的分子条形编码 |
| EP4180535A1 (en) | 2015-03-30 | 2023-05-17 | Becton, Dickinson and Company | Methods and compositions for combinatorial barcoding |
| WO2016172373A1 (en) | 2015-04-23 | 2016-10-27 | Cellular Research, Inc. | Methods and compositions for whole transcriptome amplification |
| EP4368715A2 (en) * | 2015-08-28 | 2024-05-15 | Illumina, Inc. | Nucleic acid sequence analysis from single cells |
| JP6940484B2 (ja) | 2015-09-11 | 2021-09-29 | セルラー リサーチ， インコーポレイテッド | ライブラリー正規化のための方法および組成物 |
| US10870848B2 (en) | 2015-09-15 | 2020-12-22 | Takara Bio Usa, Inc. | Methods for preparing a next generation sequencing (NGS) library from a ribonucleic acid (RNA) sample and compositions for practicing the same |
| CN105926043B (zh) * | 2016-04-19 | 2018-08-28 | 苏州贝康医疗器械有限公司 | 一种提高孕妇血浆游离dna测序文库中胎儿游离dna占比的方法 |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| JPWO2017204294A1 (ja) * | 2016-05-25 | 2019-02-21 | 株式会社ニコン | 標的生体分子の特定方法、標的生体分子特定用ビーズ、ビーズのセット、及び標的生体分子特定装置 |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| US11293021B1 (en) | 2016-06-23 | 2022-04-05 | Inscripta, Inc. | Automated cell processing methods, modules, instruments, and systems |
| CA3033506A1 (en) * | 2016-08-10 | 2018-02-15 | President And Fellows Of Harvard College | Methods of de novo assembly of barcoded genomic dna fragments |
| KR102638006B1 (ko) | 2016-09-26 | 2024-02-20 | 셀룰러 리서치, 인크. | 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정 |
| IL266197B2 (en) | 2016-10-24 | 2024-03-01 | Geneinfosec Inc | Hiding information contained within nucleic acids |
| US20180127819A1 (en) * | 2016-11-09 | 2018-05-10 | Qiagen Waltham, Inc. | Enhancing Sequencing Performance in Sequencing-by-Synthesis |
| US10982351B2 (en) | 2016-12-23 | 2021-04-20 | Grail, Inc. | Methods for high efficiency library preparation using double-stranded adapters |
| US10711269B2 (en) * | 2017-01-18 | 2020-07-14 | Agilent Technologies, Inc. | Method for making an asymmetrically-tagged sequencing library |
| EP3577232A1 (en) | 2017-02-01 | 2019-12-11 | Cellular Research, Inc. | Selective amplification using blocking oligonucleotides |
| WO2018152129A1 (en) * | 2017-02-16 | 2018-08-23 | Takara Bio Usa, Inc. | Methods of preparing nucleic acid libraries and compositions and kits for practicing the same |
| US11274344B2 (en) | 2017-03-30 | 2022-03-15 | Grail, Inc. | Enhanced ligation in sequencing library preparation |
| WO2018183897A1 (en) | 2017-03-31 | 2018-10-04 | Grail, Inc. | Higher target capture efficiency using probe extension |
| KR102438495B1 (ko) | 2017-06-05 | 2022-09-01 | 백톤 디킨슨 앤드 컴퍼니 | 단일 세포를 위한 샘플 인덱싱 |
| CN107502607A (zh) * | 2017-06-20 | 2017-12-22 | 浙江大学 | 一种大量组织、细胞样本mRNA的分子条形码标记、文库构建、测序的方法 |
| US9982279B1 (en) | 2017-06-23 | 2018-05-29 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| US10011849B1 (en) | 2017-06-23 | 2018-07-03 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| WO2018235938A1 (ja) * | 2017-06-23 | 2018-12-27 | 国立研究開発法人理化学研究所 | 核酸をシークエンシングする方法および解析する方法 |
| PT3645719T (pt) | 2017-06-30 | 2022-05-18 | Inscripta Inc | Métodos, módulos, instrumentos e sistemas automatizados de processamento de células |
| EP3651903A4 (en) * | 2017-08-29 | 2021-06-16 | Bio-Rad Laboratories, Inc. | SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS |
| WO2019061196A1 (zh) * | 2017-09-28 | 2019-04-04 | 深圳华大生命科学研究院 | 一种用于检测的pcr引物及其应用 |
| US11701656B2 (en) | 2018-01-02 | 2023-07-18 | The Regents Of The University Of Michigan | Multi-droplet capture |
| CA3096161A1 (en) * | 2018-03-28 | 2019-10-03 | Berkeley Lights, Inc. | Methods for preparation of nucleic acid sequencing libraries |
| US10526598B2 (en) | 2018-04-24 | 2020-01-07 | Inscripta, Inc. | Methods for identifying T-cell receptor antigens |
| US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
| EP3788171B1 (en) | 2018-05-03 | 2023-04-05 | Becton, Dickinson and Company | High throughput multiomics sample analysis |
| WO2019213237A1 (en) | 2018-05-03 | 2019-11-07 | Becton, Dickinson And Company | Molecular barcoding on opposite transcript ends |
| CN108611346A (zh) * | 2018-05-06 | 2018-10-02 | 湖南大地同年生物科技有限公司 | 一种单细胞基因表达量检测文库的构建方法 |
| CA3108767A1 (en) | 2018-06-30 | 2020-01-02 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| EP3821011B1 (en) * | 2018-07-11 | 2023-04-26 | Cygnus Biosciences (Beijing) Co., Ltd. | Transposome enabled dna/rna-sequencing (ted rna-seq) |
| KR20210043634A (ko) | 2018-08-15 | 2021-04-21 | 일루미나, 인코포레이티드 | 라이브러리 인리치먼트를 개선하기 위한 조성물 및 방법 |
| CN110835783B (zh) * | 2018-08-17 | 2023-06-20 | 深圳华大生命科学研究院 | 用于长读长高质量测序的核酸文库的构建方法、测序方法及试剂 |
| EP4249651A3 (en) | 2018-08-20 | 2023-10-18 | Bio-Rad Laboratories, Inc. | Nucleotide sequence generation by barcode bead-colocalization in partitions |
| US11639517B2 (en) | 2018-10-01 | 2023-05-02 | Becton, Dickinson And Company | Determining 5′ transcript sequences |
| WO2020092591A1 (en) * | 2018-11-01 | 2020-05-07 | Illumina, Inc. | Methods and compositions for somatic variant detection |
| WO2020097315A1 (en) | 2018-11-08 | 2020-05-14 | Cellular Research, Inc. | Whole transcriptome analysis of single cells using random priming |
| EP3887540B1 (en) * | 2018-11-30 | 2023-08-23 | Illumina, Inc. | Analysis of multiple analytes using a single assay |
| US11492660B2 (en) | 2018-12-13 | 2022-11-08 | Becton, Dickinson And Company | Selective extension in single cell whole transcriptome analysis |
| BR112021006095A2 (pt) * | 2018-12-17 | 2021-07-20 | Illumina, Inc. | métodos e meios para preparação de uma biblioteca para sequenciamento |
| KR20210107618A (ko) * | 2018-12-21 | 2021-09-01 | 일루미나, 인코포레이티드 | 뉴클레아제 기반 rna 고갈 |
| WO2020154247A1 (en) | 2019-01-23 | 2020-07-30 | Cellular Research, Inc. | Oligonucleotides associated with antibodies |
| US10815467B2 (en) | 2019-03-25 | 2020-10-27 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
| US11001831B2 (en) | 2019-03-25 | 2021-05-11 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
| US10633693B1 (en) | 2019-04-16 | 2020-04-28 | Celsee Diagnostics, Inc. | System and method for leakage control in a particle capture system |
| US11273439B2 (en) | 2019-05-07 | 2022-03-15 | Bio-Rad Laboratories, Inc. | System and method for target material retrieval from microwells |
| AU2020267743B2 (en) | 2019-05-07 | 2023-04-06 | Bio-Rad Laboratories, Inc. | System and method for automated single cell processing |
| CA3139122C (en) | 2019-06-06 | 2023-04-25 | Inscripta, Inc. | Curing for recursive nucleic acid-guided cell editing |
| EP3982716A4 (en) | 2019-06-14 | 2023-09-06 | Bio-Rad Laboratories, Inc. | SYSTEM AND METHOD FOR AUTOMATIC PROCESSING OF INDIVIDUAL CELLS AND ANALYZES |
| CN110257479A (zh) * | 2019-06-25 | 2019-09-20 | 北京全式金生物技术有限公司 | 一种快速构建rna 3’端基因表达文库的方法 |
| CN114051534A (zh) | 2019-07-22 | 2022-02-15 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| JP7306155B2 (ja) | 2019-08-22 | 2023-07-11 | スズキ株式会社 | 車両用動力伝達装置 |
| US11773436B2 (en) | 2019-11-08 | 2023-10-03 | Becton, Dickinson And Company | Using random priming to obtain full-length V(D)J information for immune repertoire sequencing |
| US11203762B2 (en) | 2019-11-19 | 2021-12-21 | Inscripta, Inc. | Methods for increasing observed editing in bacteria |
| CA3157127A1 (en) | 2019-12-18 | 2021-06-24 | Aamir MIR | Cascade/dcas3 complementation assays for in vivo detection of nucleic acid-guided nuclease edited cells |
| EP4090763A1 (en) | 2020-01-13 | 2022-11-23 | Becton Dickinson and Company | Methods and compositions for quantitation of proteins and rna |
| EP4095256A4 (en) * | 2020-01-23 | 2023-03-15 | DGI Tech (Qing Dao) Co., Limited | DROPLET MICROFLUIDIC-BASED SINGLE CELL SEQUENCING AND APPLICATIONS |
| AU2021213705A1 (en) | 2020-01-27 | 2022-06-16 | Inscripta, Inc. | Electroporation modules and instrumentation |
| US11504719B2 (en) | 2020-03-12 | 2022-11-22 | Bio-Rad Laboratories, Inc. | System and method for receiving and delivering a fluid for sample processing |
| US20210332388A1 (en) | 2020-04-24 | 2021-10-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells |
| CN115605614A (zh) | 2020-05-14 | 2023-01-13 | 贝克顿迪金森公司（Us） | 用于免疫组库谱分析的引物 |
| US20210381007A1 (en) * | 2020-06-04 | 2021-12-09 | Inscripta, Inc. | Methods and compositions for crispr editing of cells and correlating the edits to a resulting cellular nucleic acid profile |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| WO2022021279A1 (zh) * | 2020-07-31 | 2022-02-03 | 北京寻因生物科技有限公司 | 多种核酸共标记支持物及其制作方法与应用 |
| AU2021320307A1 (en) | 2020-08-06 | 2023-02-16 | Illumina Cambridge Limited | Preparation of RNA and DNA sequencing libraries using bead-linked transposomes |
| WO2022060749A1 (en) | 2020-09-15 | 2022-03-24 | Inscripta, Inc. | Crispr editing to embed nucleic acid landing pads into genomes of live cells |
| WO2022067494A1 (en) * | 2020-09-29 | 2022-04-07 | Singleron (Nanjing) Biotechnologies, Ltd. | Method for detection of whole transcriptome in single cells |
| US11512297B2 (en) | 2020-11-09 | 2022-11-29 | Inscripta, Inc. | Affinity tag for recombination protein recruitment |
| CN116635533A (zh) | 2020-11-20 | 2023-08-22 | 贝克顿迪金森公司 | 高表达的蛋白和低表达的蛋白的谱分析 |
| EP4271802A1 (en) | 2021-01-04 | 2023-11-08 | Inscripta, Inc. | Mad nucleases |
| WO2022150269A1 (en) | 2021-01-07 | 2022-07-14 | Inscripta, Inc. | Mad nucleases |
| US11884924B2 (en) | 2021-02-16 | 2024-01-30 | Inscripta, Inc. | Dual strand nucleic acid-guided nickase editing |
| WO2022188054A1 (en) * | 2021-03-10 | 2022-09-15 | Nanjing University | Methods and reagents for sample multiplexing for high throughput single-cell rna sequencing |
| EP4314283A1 (en) | 2021-03-31 | 2024-02-07 | Illumina, Inc. | Methods of preparing directional tagmentation sequencing libraries using transposon-based technology with unique molecular identifiers for error correction |
| IL308886A (en) * | 2021-06-11 | 2024-01-01 | Cellanome Inc | Systems and methods for analyzing biological samples |
| WO2023159416A1 (zh) * | 2022-02-24 | 2023-08-31 | 浙江大学 | 一种单细胞转录组测序方法及其应用 |
Family Cites Families (73)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2044616A1 (en) | 1989-10-26 | 1991-04-27 | Roger Y. Tsien | Dna sequencing |
| US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
| AU7398996A (en) | 1995-10-11 | 1997-04-30 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and method |
| CA2260991C (en) | 1997-11-18 | 2009-03-24 | Bio-Rad Laboratories, Inc. | Multiplex flow immunoassays with magnetic particles as solid phase |
| US6565727B1 (en) | 1999-01-25 | 2003-05-20 | Nanolytics, Inc. | Actuators for microfluidics without moving parts |
| US6294063B1 (en) | 1999-02-12 | 2001-09-25 | Board Of Regents, The University Of Texas System | Method and apparatus for programmable fluidic processing |
| US6277607B1 (en) * | 1999-05-24 | 2001-08-21 | Sanjay Tyagi | High specificity primers, amplification methods and kits |
| DE60027576T2 (de) | 1999-08-17 | 2007-05-03 | Luminex Corp., Austin | Verkapselung von fluoreszierenden partikeln |
| DE60040603D1 (de) | 1999-08-17 | 2008-12-04 | Luminex Corp | Verfahren zur analyse einer mehrzahl von proben verschiedenen ursprungs auf einen analyten |
| CN100462433C (zh) | 2000-07-07 | 2009-02-18 | 维西根生物技术公司 | 实时序列测定 |
| WO2002007503A1 (en) | 2000-07-25 | 2002-01-31 | The Regents Of The University Of California | Electrowetting-driven micropumping |
| US6773566B2 (en) | 2000-08-31 | 2004-08-10 | Nanolytics, Inc. | Electrostatic actuators for microfluidics and methods for using same |
| WO2002044425A2 (en) | 2000-12-01 | 2002-06-06 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| CA2472029C (en) | 2001-11-26 | 2014-04-15 | Keck Graduate Institute | Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| FR2841063B1 (fr) | 2002-06-18 | 2004-09-17 | Commissariat Energie Atomique | Dispositif de deplacement de petits volumes de liquide le long d'un micro-catenaire par des forces electrostatiques |
| WO2004018497A2 (en) | 2002-08-23 | 2004-03-04 | Solexa Limited | Modified nucleotides for polynucleotide sequencing |
| US6911132B2 (en) | 2002-09-24 | 2005-06-28 | Duke University | Apparatus for manipulating droplets by electrowetting-based techniques |
| US7547380B2 (en) | 2003-01-13 | 2009-06-16 | North Carolina State University | Droplet transportation devices and methods having a fluid surface |
| WO2005003304A2 (en) * | 2003-06-20 | 2005-01-13 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
| CA2543350C (en) | 2003-10-24 | 2011-04-26 | Adhesives Research, Inc. | Disintegratable films for diagnostic devices |
| DE602004021624D1 (de) | 2003-11-17 | 2009-07-30 | Koninkl Philips Electronics Nv | System zur handhabung einer fluidmenge |
| GB0400584D0 (en) | 2004-01-12 | 2004-02-11 | Solexa Ltd | Nucleic acid chacterisation |
| JP2007518991A (ja) | 2004-01-14 | 2007-07-12 | ルミネックス・コーポレーション | ダイナミックレンジを拡大する方法及びシステム |
| FR2866493B1 (fr) | 2004-02-16 | 2010-08-20 | Commissariat Energie Atomique | Dispositif de controle du deplacement d'une goutte entre deux ou plusieurs substrats solides |
| FR2872715B1 (fr) | 2004-07-08 | 2006-11-17 | Commissariat Energie Atomique | Microreacteur goutte |
| FR2872809B1 (fr) | 2004-07-09 | 2006-09-15 | Commissariat Energie Atomique | Methode d'adressage d'electrodes |
| JP2006058031A (ja) | 2004-08-17 | 2006-03-02 | Hitachi High-Technologies Corp | 化学分析装置 |
| CN101914620B (zh) | 2004-09-17 | 2014-02-12 | 加利福尼亚太平洋生命科学公司 | 核酸测序的方法 |
| EP2244270B1 (en) | 2005-01-20 | 2012-06-06 | Luminex Corporation | Microspheres having fluorescent and magnetic properties |
| US7458661B2 (en) | 2005-01-25 | 2008-12-02 | The Regents Of The University Of California | Method and apparatus for promoting the complete transfer of liquid drops from a nozzle |
| JP5897780B2 (ja) | 2005-01-28 | 2016-03-30 | デューク ユニバーシティ | プリント回路基板上の液滴操作装置及び方法 |
| US20070023292A1 (en) | 2005-07-26 | 2007-02-01 | The Regents Of The University Of California | Small object moving on printed circuit board |
| EP2261635B1 (en) | 2005-09-21 | 2017-05-03 | Luminex Corporation | Methods and systems for image data processing |
| US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
| WO2007103859A2 (en) | 2006-03-03 | 2007-09-13 | Luminex Corporation | Methods, products, and kits for identifying an analyte in a sample |
| EP2018622B1 (en) | 2006-03-31 | 2018-04-25 | Illumina, Inc. | Systems for sequence by synthesis analysis |
| US7901947B2 (en) | 2006-04-18 | 2011-03-08 | Advanced Liquid Logic, Inc. | Droplet-based particle sorting |
| WO2010027894A2 (en) | 2008-08-27 | 2010-03-11 | Advanced Liquid Logic, Inc. | Droplet actuators, modified fluids and methods |
| US7439014B2 (en) | 2006-04-18 | 2008-10-21 | Advanced Liquid Logic, Inc. | Droplet-based surface modification and washing |
| CA2680061C (en) | 2006-04-18 | 2015-10-13 | Duke University | Droplet-based biochemistry |
| JP4881950B2 (ja) | 2006-07-10 | 2012-02-22 | 株式会社日立ハイテクノロジーズ | 液体搬送デバイス |
| EP2071927A2 (en) | 2006-09-28 | 2009-06-24 | Illumina, Inc. | Compositions and methods for nucleotide sequencing |
| WO2008051530A2 (en) | 2006-10-23 | 2008-05-02 | Pacific Biosciences Of California, Inc. | Polymerase enzymes and reagents for enhanced nucleic acid sequencing |
| US9266076B2 (en) | 2006-11-02 | 2016-02-23 | The Regents Of The University Of California | Method and apparatus for real-time feedback control of electrical manipulation of droplets on chip |
| WO2008074023A2 (en) | 2006-12-13 | 2008-06-19 | Luminex Corporation | Systems and methods for multiplex analysis of pcr in real time |
| WO2008093098A2 (en) | 2007-02-02 | 2008-08-07 | Illumina Cambridge Limited | Methods for indexing samples and sequencing multiple nucleotide templates |
| WO2008098236A2 (en) | 2007-02-09 | 2008-08-14 | Advanced Liquid Logic, Inc. | Droplet actuator devices and methods employing magnetic beads |
| US8872527B2 (en) | 2007-02-15 | 2014-10-28 | Advanced Liquid Logic, Inc. | Capacitance detection in a droplet actuator |
| US8093062B2 (en) | 2007-03-22 | 2012-01-10 | Theodore Winger | Enzymatic assays using umbelliferone substrates with cyclodextrins in droplets in oil |
| US20100048410A1 (en) | 2007-03-22 | 2010-02-25 | Advanced Liquid Logic, Inc. | Bead Sorting on a Droplet Actuator |
| US20100130369A1 (en) | 2007-04-23 | 2010-05-27 | Advanced Liquid Logic, Inc. | Bead-Based Multiplexed Analytical Methods and Instrumentation |
| US20080283414A1 (en) | 2007-05-17 | 2008-11-20 | Monroe Charles W | Electrowetting devices |
| WO2009003184A1 (en) | 2007-06-27 | 2008-12-31 | Digital Biosystems | Digital microfluidics based apparatus for heat-exchanging chemical processes |
| US20110303542A1 (en) | 2007-08-08 | 2011-12-15 | Advanced Liquid Logic, Inc. | Use of Additives for Enhancing Droplet Operations |
| US8093064B2 (en) | 2008-05-15 | 2012-01-10 | The Regents Of The University Of California | Method for using magnetic particles in droplet microfluidics |
| DK2963709T3 (en) | 2008-10-24 | 2017-09-11 | Epicentre Tech Corp | TRANSPOSON-END COMPOSITIONS AND PROCEDURES FOR MODIFICATION OF NUCLEIC ACIDS |
| US9080211B2 (en) * | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| US20110311980A1 (en) * | 2008-12-15 | 2011-12-22 | Advanced Liquid Logic, Inc. | Nucleic Acid Amplification and Sequencing on a Droplet Actuator |
| EP3495498B1 (en) * | 2009-03-30 | 2021-10-27 | Illumina, Inc. | Gene expression analysis in single cells |
| WO2011002957A2 (en) | 2009-07-01 | 2011-01-06 | Advanced Liquid Logic, Inc. | Droplet actuator devices and methods |
| US9902950B2 (en) | 2010-10-08 | 2018-02-27 | President And Fellows Of Harvard College | High-throughput single cell barcoding |
| EP3144396B1 (en) * | 2010-10-27 | 2020-01-01 | President and Fellows of Harvard College | Methods of use of toehold hairpin primer |
| PL2697397T3 (pl) | 2011-04-15 | 2017-08-31 | The Johns Hopkins University | System bezpiecznego sekwencjonowania |
| US9005935B2 (en) | 2011-05-23 | 2015-04-14 | Agilent Technologies, Inc. | Methods and compositions for DNA fragmentation and tagging by transposases |
| EP2718465B1 (en) | 2011-06-09 | 2022-04-13 | Illumina, Inc. | Method of making an analyte array |
| WO2013085918A1 (en) | 2011-12-05 | 2013-06-13 | The Regents Of The University Of California | Methods and compostions for generating polynucleic acid fragments |
| WO2013117595A2 (en) | 2012-02-07 | 2013-08-15 | Illumina Cambridge Limited | Targeted enrichment and amplification of nucleic acids on a support |
| NO2694769T3 (ja) | 2012-03-06 | 2018-03-03 | ||
| EP2898096B1 (en) * | 2012-09-21 | 2024-02-14 | The Broad Institute, Inc. | Methods for labeling of rnas |
| WO2014201273A1 (en) * | 2013-06-12 | 2014-12-18 | The Broad Institute, Inc. | High-throughput rna-seq |
| KR20230074639A (ko) * | 2013-08-28 | 2023-05-30 | 벡톤 디킨슨 앤드 컴퍼니 | 대량의 동시 단일 세포 분석 |
| PL3089822T3 (pl) * | 2013-12-30 | 2022-09-19 | Atreca, Inc. | Analiza kwasów nukleinowych powiązanych z pojedynczymi komórkami z użyciem kodów kreskowych kwasów nukleinowych |
-
2015
- 2015-04-28 EP EP20154378.2A patent/EP3680333A1/en active Pending
- 2015-04-28 AU AU2015253299A patent/AU2015253299B2/en active Active
- 2015-04-28 US US15/307,329 patent/US20170044525A1/en not_active Abandoned
- 2015-04-28 SG SG11201609053YA patent/SG11201609053YA/en unknown
- 2015-04-28 CA CA2947426A patent/CA2947426C/en active Active
- 2015-04-28 JP JP2016565421A patent/JP6412954B2/ja active Active
- 2015-04-28 CN CN201580033171.7A patent/CN106459967A/zh active Pending
- 2015-04-28 EP EP15721102.0A patent/EP3137601B1/en active Active
- 2015-04-28 CA CA3060708A patent/CA3060708A1/en active Pending
- 2015-04-28 SG SG10201912516SA patent/SG10201912516SA/en unknown
- 2015-04-28 WO PCT/US2015/028062 patent/WO2015168161A2/en active Application Filing
-
2018
- 2018-09-07 AU AU2018226493A patent/AU2018226493B2/en active Active
-
2020
- 2020-10-23 US US17/078,900 patent/US20210079386A1/en active Pending
-
2021
- 2021-05-20 AU AU2021203246A patent/AU2021203246B2/en active Active
-
2024
- 2024-02-28 AU AU2024201323A patent/AU2024201323A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP3680333A1 (en) | 2020-07-15 |
| EP3137601B1 (en) | 2020-04-08 |
| WO2015168161A2 (en) | 2015-11-05 |
| US20170044525A1 (en) | 2017-02-16 |
| AU2018226493A1 (en) | 2018-09-27 |
| AU2024201323A1 (en) | 2024-03-21 |
| AU2021203246B2 (en) | 2023-11-30 |
| WO2015168161A3 (en) | 2015-12-30 |
| AU2015253299B2 (en) | 2018-06-14 |
| CA2947426A1 (en) | 2015-11-05 |
| US20210079386A1 (en) | 2021-03-18 |
| SG10201912516SA (en) | 2020-02-27 |
| AU2015253299A1 (en) | 2016-11-17 |
| AU2021203246A1 (en) | 2021-06-10 |
| CN106459967A (zh) | 2017-02-22 |
| AU2018226493B2 (en) | 2021-02-25 |
| JP6412954B2 (ja) | 2018-10-24 |
| EP3137601A2 (en) | 2017-03-08 |
| CA3060708A1 (en) | 2015-11-05 |
| JP2017514487A (ja) | 2017-06-08 |
| CA2947426C (en) | 2020-01-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| SG10201912516SA (en) | Multiplexed single cell gene expression analysis using template switch and tagmentation | |
| IL255157A0 (en) | Microfluidic cell culture | |
| GB2556436B (en) | Cell population analysis | |
| SG11201703493SA (en) | Cell culture insert | |
| PL3113883T3 (pl) | Układ sortujący komórki z wykorzystaniem mikrofabrykowanych składowych | |
| GB2598259B (en) | Cell Population Analysis | |
| GB201611982D0 (en) | Cell culture | |
| HK1253068A1 (zh) | 微流體細胞培養 | |
| PL3134724T3 (pl) | Komórka elektrochemiczna | |
| SG11201702377QA (en) | Blood sample management using open cell foam | |
| IL269168A (en) | single cell diagnosis | |
| SG10202004286UA (en) | Integrated single cell sequencing | |
| HUE059470T2 (hu) | Sejttenyészet | |
| SG11201606297UA (en) | Blood sample management using open cell foam | |
| GB201714180D0 (en) | Transduced cell cryoformulation | |
| PL3287521T3 (pl) | Hodowla komórkowa | |
| TWI563082B (en) | Cell culture device | |
| SG10202010002RA (en) | Cell culture | |
| GB201421342D0 (en) | Cell culture | |
| GB201420709D0 (en) | Electrochemical cells and components thereof | |
| GB201411798D0 (en) | Electrochemical cell | |
| GB201413395D0 (en) | Cell separation methods | |
| GB201403494D0 (en) | Identifying cell features | |
| GB201406100D0 (en) | Epigenetic rejuvenation - ips cell method |