SG11201508642WA - Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones - Google Patents
Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperonesInfo
- Publication number
- SG11201508642WA SG11201508642WA SG11201508642WA SG11201508642WA SG11201508642WA SG 11201508642W A SG11201508642W A SG 11201508642WA SG 11201508642W A SG11201508642W A SG 11201508642WA SG 11201508642W A SG11201508642W A SG 11201508642WA SG 11201508642W A SG11201508642W A SG 11201508642WA
- Authority
- SG
- Singapore
- Prior art keywords
- expression
- biologically active
- elevated levels
- synthesis system
- active proteins
- Prior art date
Links
- 102000005431 Molecular Chaperones Human genes 0.000 title 1
- 108010006519 Molecular Chaperones Proteins 0.000 title 1
- 230000001580 bacterial effect Effects 0.000 title 1
- 230000015572 biosynthetic process Effects 0.000 title 1
- 239000000284 extract Substances 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 238000003786 synthesis reaction Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361813914P | 2013-04-19 | 2013-04-19 | |
| US201461937069P | 2014-02-07 | 2014-02-07 | |
| PCT/US2014/034643 WO2014172631A2 (en) | 2013-04-19 | 2014-04-18 | Expression of biologically active proteins in a bacterial cell-free synthesis system using bacterial cells transformed to exhibit elevated levels of chaperone expression |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201508642WA true SG11201508642WA (en) | 2015-11-27 |
Family
ID=50733442
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201508642WA SG11201508642WA (en) | 2013-04-19 | 2014-04-18 | Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones |
| SG10202108497TA SG10202108497TA (en) | 2013-04-19 | 2014-04-18 | Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10202108497TA SG10202108497TA (en) | 2013-04-19 | 2014-04-18 | Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones |
Country Status (13)
| Country | Link |
|---|---|
| US (3) | US10190145B2 (https=) |
| EP (1) | EP2986731B1 (https=) |
| JP (2) | JP6463732B2 (https=) |
| KR (1) | KR102289258B1 (https=) |
| CN (1) | CN105283556B (https=) |
| AU (1) | AU2014253785B2 (https=) |
| CA (1) | CA2909516C (https=) |
| DK (1) | DK2986731T3 (https=) |
| ES (1) | ES2922358T3 (https=) |
| HU (1) | HUE059565T2 (https=) |
| IL (1) | IL242148B (https=) |
| SG (2) | SG11201508642WA (https=) |
| WO (1) | WO2014172631A2 (https=) |
Families Citing this family (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG11201508642WA (en) * | 2013-04-19 | 2015-11-27 | Sutro Biopharma Inc | Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones |
| US10316322B2 (en) * | 2014-07-02 | 2019-06-11 | Sutro Biopharma, Inc. | High growth capacity auxotrophic Escherichia coli and methods of use |
| WO2016073791A1 (en) | 2014-11-05 | 2016-05-12 | Genentech, Inc. | Methods of producing two chain proteins in bacteria |
| US10066002B2 (en) * | 2014-11-05 | 2018-09-04 | Genentech, Inc. | Methods of producing two chain proteins in bacteria |
| US10487133B2 (en) | 2014-12-19 | 2019-11-26 | Sutro Biopharma, Inc. | Codon optimization for titer and fidelity improvement |
| MX391051B (es) * | 2014-12-30 | 2025-03-21 | Celgene Corp | Anticuerpos anti-cd47 y usos de los mismos. |
| HRP20192217T1 (hr) * | 2015-03-06 | 2020-02-21 | F. Hoffmann - La Roche Ag | ULTRA-PROČIŠĆENI DsbA I DsbC TE POSTUPCI NJIHOVE IZRADE I UPORABE |
| US20170029798A1 (en) * | 2015-07-27 | 2017-02-02 | Cellivery Therapeutics, Inc. | Development of Improved Cell-Permeable (iCP) Parkin Recombinant Protein as a Protein-Based Anti-Neurodegenerative Agent for the Treatment of Parkinson's Disease-Associated Phenotypes by Utilizing BBB-Penetrating Protein Delivery System MITT, Enabled by Advanced Macromolecule Transduction Domain (aMTD) |
| KR20180048731A (ko) * | 2015-08-20 | 2018-05-10 | 제넨테크, 인크. | 재조합 폴리펩티드를 제조하기 위한 fkpa의 정제 및 그의 용도 |
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| CN110366428B (zh) | 2016-12-30 | 2024-05-17 | Vaxcyte公司 | 具有非天然氨基酸的多肽-抗原缀合物 |
| US11951165B2 (en) | 2016-12-30 | 2024-04-09 | Vaxcyte, Inc. | Conjugated vaccine carrier proteins |
| US12227778B2 (en) | 2016-12-30 | 2025-02-18 | Nature's Toolbox, Inc. | Cell-free expression system having novel inorganic polyphosphate-based energy regeneration |
| JP7325341B2 (ja) | 2017-07-11 | 2023-08-14 | シンソークス，インク． | 非天然ヌクレオチドの組み込み及びその方法 |
| EP3652318A1 (en) | 2017-07-11 | 2020-05-20 | Actym Therapeutics, Inc. | Engineered immunostimulatory bacterial strains and uses thereof |
| KR20240141853A (ko) | 2017-08-03 | 2024-09-27 | 신톡스, 인크. | 자가면역 질환의 치료를 위한 사이토카인 접합체 |
| JP2020533018A (ja) * | 2017-08-11 | 2020-11-19 | シンビトロバイオ， インコーポレイテッド | 改善されたインビトロ転写／翻訳（ｔｘｔｌ）システムおよびその使用 |
| WO2019127469A1 (zh) * | 2017-12-29 | 2019-07-04 | 康码（上海）生物科技有限公司 | 一种高效的能源再生体系(bes)、试剂盒及其制备方法 |
| CN112004547A (zh) | 2018-02-26 | 2020-11-27 | 新索思股份有限公司 | Il-15缀合物及其用途 |
| JP7340591B2 (ja) | 2018-07-11 | 2023-09-07 | アクティム・セラピューティクス・インコーポレイテッド | 遺伝子操作された免疫刺激性細菌菌株およびその使用 |
| EP3877407B1 (en) * | 2018-11-05 | 2026-03-11 | F. Hoffmann-La Roche AG | Methods of producing two chain proteins in prokaryotic host cells |
| ES2944837T3 (es) * | 2018-11-08 | 2023-06-26 | Sutro Biopharma Inc | Cepas de E. coli con citoplasma oxidativo |
| SG11202102930YA (en) | 2018-11-08 | 2021-04-29 | Synthorx Inc | Interleukin 10 conjugates and uses thereof |
| CN109371049A (zh) * | 2018-11-14 | 2019-02-22 | 天津大学 | 分子伴侣在促进单克隆抗体的形成和/或提高单克隆抗体的表达量中的应用 |
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| WO2021178001A1 (en) * | 2020-03-05 | 2021-09-10 | Curie Co. Inc. | Methods for cell free protein expression of mature polypeptides derived from zymogens and proproteins |
| CN114555128A (zh) | 2019-08-15 | 2022-05-27 | 新索思股份有限公司 | 采用il-2缀合物的免疫肿瘤学组合疗法 |
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| US20230032072A1 (en) * | 2019-11-08 | 2023-02-02 | Korea Institute Of Ocean Science & Technology | Novel human epidermal growth factor-tf fusion protein and use thereof |
| CA3175683A1 (en) * | 2020-04-29 | 2021-11-04 | Xueyang FENG | Compositions and methods for enhancing recombinant biosynthesis of cannabinoids |
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| TW202245843A (zh) | 2021-02-12 | 2022-12-01 | 美商欣爍克斯公司 | Ｉｌ－２接合物及西米普利單抗（ｃｅｍｉｐｌｉｍａｂ）之皮膚癌組合療法 |
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| EP4346904A1 (en) | 2021-06-03 | 2024-04-10 | Synthorx, Inc. | Head and neck cancer combination therapy comprising an il-2 conjugate and cetuximab |
| EP4452327A1 (en) | 2021-12-20 | 2024-10-30 | Synthorx, Inc. | Head and neck cancer combination therapy comprising an il-2 conjugate and pembrolizumab |
| WO2023122750A1 (en) | 2021-12-23 | 2023-06-29 | Synthorx, Inc. | Cancer combination therapy with il-2 conjugates and cetuximab |
| CN116555061A (zh) * | 2022-01-28 | 2023-08-08 | 北京师范大学 | 一种酿酒酵母突变体及其在制备酒精中的应用 |
| US12163190B2 (en) * | 2022-06-17 | 2024-12-10 | Insitro, Inc. | In situ sequencing of RNA transcripts with non-uniform 5 prime ends |
| CN115028691B (zh) * | 2022-06-29 | 2024-09-10 | 青岛硕景生物科技有限公司 | 一种检测试纸用的独立质控系统、杂交瘤细胞株、质控线用包被单克隆抗体及其应用 |
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| CN116925196B (zh) * | 2023-06-08 | 2025-04-18 | 齐鲁工业大学(山东省科学院) | 小分子热休克蛋白rsp_1572在提高宿主菌环境耐受性中的应用 |
| WO2025158385A1 (en) | 2024-01-25 | 2025-07-31 | Genzyme Corporation | Pegylated il-2 for suppressing adaptive immune response to gene therapy |
| CN118048325B (zh) * | 2024-04-16 | 2024-07-16 | 天津凯莱英生物科技有限公司 | 酶的高通量筛选方法及酶库的构建方法 |
| WO2025218039A1 (zh) * | 2024-04-16 | 2025-10-23 | 天津凯莱英生物科技有限公司 | 酶的高通量筛选方法及酶库的构建方法 |
| WO2025240255A1 (en) * | 2024-05-11 | 2025-11-20 | Biodiscovery, Llc | Systems and methods of cell-free protein expression |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10145694A1 (de) * | 2001-09-17 | 2003-04-03 | Roche Diagnostics Gmbh | Verfahren zur Erhöhung der Löslichkeit, der Expressionsrate und der Aktivität von Proteinen während der rekombinanten Herstellung |
| US20060134739A1 (en) * | 2003-03-11 | 2006-06-22 | Chatterjee Deb K | Synthesis of proteins by cell-free protein expression |
| JP2005253432A (ja) * | 2004-03-15 | 2005-09-22 | Sekisui Chem Co Ltd | タンパク質の生産方法、並びにそれに使用するための組成物、試薬及びキット |
| JP4561243B2 (ja) * | 2004-08-27 | 2010-10-13 | 株式会社豊田中央研究所 | 無細胞タンパク質合成系のための媒体 |
| WO2008066583A2 (en) * | 2006-06-29 | 2008-06-05 | The Board Of Trustees Of The Leland Stanford Junior University | Cell-free synthesis of proteins containing unnatural amino acids |
| CA2673765A1 (en) * | 2007-01-18 | 2008-07-24 | The Board Of Trustees Of The Leland Stanford Junior University | Enhanced cell-free synthesis of active proteins containing disulfide bonds |
| JP5798489B2 (ja) * | 2009-01-12 | 2015-10-21 | ストロ バイオファーマ， インコーポレイテッド | インビトロタンパク質合成システムを用いて非天然アミノ酸をタンパク質に選択的に導入するためのモノチャージシステム |
| US20110136169A1 (en) * | 2009-11-20 | 2011-06-09 | Rutgers, The State University Of New Jersey | Expression system compositions and methods |
| CN102732548A (zh) * | 2012-05-16 | 2012-10-17 | 中国农业大学 | 一种表达蛇毒激肽原酶的高效麦胚无细胞蛋白合成系统的建立及应用 |
| PL2906592T3 (pl) * | 2012-10-12 | 2019-07-31 | Sutro Biopharma, Inc. | Proteolityczna inaktywacja wybranych białek w ekstraktach bakteryjnych dla ulepszonej ekspresji |
| SG11201508642WA (en) | 2013-04-19 | 2015-11-27 | Sutro Biopharma Inc | Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones |
-
2014
- 2014-04-18 SG SG11201508642WA patent/SG11201508642WA/en unknown
- 2014-04-18 JP JP2016509127A patent/JP6463732B2/ja active Active
- 2014-04-18 DK DK14724974.2T patent/DK2986731T3/da active
- 2014-04-18 EP EP14724974.2A patent/EP2986731B1/en active Active
- 2014-04-18 US US14/256,324 patent/US10190145B2/en active Active
- 2014-04-18 HU HUE14724974A patent/HUE059565T2/hu unknown
- 2014-04-18 CA CA2909516A patent/CA2909516C/en active Active
- 2014-04-18 CN CN201480032258.8A patent/CN105283556B/zh active Active
- 2014-04-18 ES ES14724974T patent/ES2922358T3/es active Active
- 2014-04-18 KR KR1020157032515A patent/KR102289258B1/ko active Active
- 2014-04-18 SG SG10202108497TA patent/SG10202108497TA/en unknown
- 2014-04-18 WO PCT/US2014/034643 patent/WO2014172631A2/en not_active Ceased
- 2014-04-18 AU AU2014253785A patent/AU2014253785B2/en active Active
-
2015
- 2015-10-18 IL IL242148A patent/IL242148B/en active IP Right Grant
-
2018
- 2018-07-20 JP JP2018136909A patent/JP2018157837A/ja not_active Withdrawn
- 2018-12-03 US US16/208,245 patent/US10774354B2/en active Active
-
2020
- 2020-08-12 US US16/991,607 patent/US20210054429A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| ES2922358T3 (es) | 2022-09-13 |
| EP2986731A2 (en) | 2016-02-24 |
| SG10202108497TA (en) | 2021-09-29 |
| JP6463732B2 (ja) | 2019-02-06 |
| IL242148B (en) | 2019-03-31 |
| AU2014253785B2 (en) | 2018-07-19 |
| HK1220993A1 (en) | 2017-05-19 |
| US20210054429A1 (en) | 2021-02-25 |
| CN105283556A (zh) | 2016-01-27 |
| DK2986731T3 (da) | 2022-08-01 |
| JP2016518833A (ja) | 2016-06-30 |
| US20190161781A1 (en) | 2019-05-30 |
| US20140315245A1 (en) | 2014-10-23 |
| US10190145B2 (en) | 2019-01-29 |
| KR20160002913A (ko) | 2016-01-08 |
| CA2909516C (en) | 2023-03-07 |
| JP2018157837A (ja) | 2018-10-11 |
| WO2014172631A4 (en) | 2015-02-19 |
| HUE059565T2 (hu) | 2022-11-28 |
| WO2014172631A2 (en) | 2014-10-23 |
| WO2014172631A3 (en) | 2014-12-18 |
| CN105283556B (zh) | 2020-07-14 |
| CA2909516A1 (en) | 2014-10-23 |
| EP2986731B1 (en) | 2022-05-18 |
| KR102289258B1 (ko) | 2021-08-11 |
| AU2014253785A1 (en) | 2015-11-05 |
| US10774354B2 (en) | 2020-09-15 |
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