SG10201805815YA - Rna-guided gene drives - Google Patents

Rna-guided gene drives

Info

Publication number
SG10201805815YA
SG10201805815YA SG10201805815YA SG10201805815YA SG10201805815YA SG 10201805815Y A SG10201805815Y A SG 10201805815YA SG 10201805815Y A SG10201805815Y A SG 10201805815YA SG 10201805815Y A SG10201805815Y A SG 10201805815YA SG 10201805815Y A SG10201805815Y A SG 10201805815YA
Authority
SG
Singapore
Prior art keywords
rna
guided
dna
gene drives
present disclosure
Prior art date
Application number
SG10201805815YA
Other languages
English (en)
Inventor
Kevin Esvelt
Andrea L Smidler
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College filed Critical Harvard College
Publication of SG10201805815YA publication Critical patent/SG10201805815YA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Catching Or Destruction (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
SG10201805815YA 2014-01-08 2015-01-08 Rna-guided gene drives SG10201805815YA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461924735P 2014-01-08 2014-01-08
US201462024642P 2014-07-15 2014-07-15

Publications (1)

Publication Number Publication Date
SG10201805815YA true SG10201805815YA (en) 2018-08-30

Family

ID=53524319

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201805815YA SG10201805815YA (en) 2014-01-08 2015-01-08 Rna-guided gene drives
SG11201605550QA SG11201605550QA (en) 2014-01-08 2015-01-08 Rna-guided gene drives

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG11201605550QA SG11201605550QA (en) 2014-01-08 2015-01-08 Rna-guided gene drives

Country Status (9)

Country Link
US (1) US10526618B2 (fr)
EP (1) EP3092310B1 (fr)
JP (2) JP6747974B2 (fr)
CN (1) CN106133141B (fr)
AU (2) AU2015204784B2 (fr)
CA (1) CA2936312A1 (fr)
SG (2) SG10201805815YA (fr)
WO (1) WO2015105928A1 (fr)
ZA (1) ZA201604874B (fr)

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013066438A2 (fr) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Évaluation et amélioration de la spécificité de clivage des nucléases
ES2741951T3 (es) 2012-12-17 2020-02-12 Harvard College Modificación por ingeniería genética del genoma humano guiada por ARN
WO2014113493A1 (fr) 2013-01-16 2014-07-24 Emory University Complexes d'acide nucléique cas9 et leurs utilisations
KR20200136508A (ko) 2013-04-16 2020-12-07 리제너론 파마슈티칼스 인코포레이티드 랫트 게놈의 표적화된 변형
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
WO2015070083A1 (fr) 2013-11-07 2015-05-14 Editas Medicine,Inc. Méthodes et compositions associées à crispr avec arng de régulation
CA2933433C (fr) 2013-12-11 2020-11-17 Regeneron Pharmaceuticals, Inc. Procedes et compositions pour la modification ciblee d'un genome
US20150165054A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting caspase-9 point mutations
WO2015200805A2 (fr) 2014-06-26 2015-12-30 Regeneron Pharmaceuticals, Inc. Procédés et compositions pour modification génétiques ciblées et procédés d'utilisation
AU2015298571B2 (en) 2014-07-30 2020-09-03 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
WO2016073559A1 (fr) * 2014-11-05 2016-05-12 The Regents Of The University Of California Procédés pour l'édition autocatalytique d'un génome et la neutralisation de l'édition autocatalytique d'un génome
CA3176380A1 (fr) 2014-11-21 2016-05-26 Regeneron Pharmaceuticals, Inc. Procedes et compositions pour modification genetique ciblee utilisant des arn guides apparies
CA2970370A1 (fr) 2014-12-24 2016-06-30 Massachusetts Institute Of Technology Crispr presentant ou associe avec un domaine de destabilisation
US10117911B2 (en) 2015-05-29 2018-11-06 Agenovir Corporation Compositions and methods to treat herpes simplex virus infections
AU2016279062A1 (en) 2015-06-18 2019-03-28 Omar O. Abudayyeh Novel CRISPR enzymes and systems
WO2017058839A1 (fr) * 2015-10-02 2017-04-06 President And Fellows Of Harvard College Systèmes de forçage génétique pour l'édition de génome à composants dépendants
US20170096649A1 (en) * 2015-10-02 2017-04-06 Agenovir Corporation Transgenic nuclease systems and methods
JP7109784B2 (ja) 2015-10-23 2022-08-01 プレジデント アンド フェローズ オブ ハーバード カレッジ 遺伝子編集のための進化したＣａｓ９蛋白質
US20190161743A1 (en) * 2016-05-09 2019-05-30 President And Fellows Of Harvard College Self-Targeting Guide RNAs in CRISPR System
WO2017196858A1 (fr) * 2016-05-09 2017-11-16 Massachusetts Institute Of Technology Procédés de conception et d'utilisation de systèmes d'entraînement de gènes
EP3494215A1 (fr) 2016-08-03 2019-06-12 President and Fellows of Harvard College Éditeurs de nucléobases d'adénosine et utilisations associées
JP7201153B2 (ja) 2016-08-09 2023-01-10 プレジデント アンド フェローズ オブ ハーバード カレッジ プログラム可能ｃａｓ９－リコンビナーゼ融合タンパク質およびその使用
WO2018035300A1 (fr) * 2016-08-17 2018-02-22 The Regents Of The University Of California Système de forçage génétique divisé par complémentation trans, destiné à supprimer des moustiques aedes aegypti
WO2018039438A1 (fr) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation d'acides aminés non naturels dans des protéines au moyen de l'édition de bases
US20190241879A1 (en) * 2016-09-09 2019-08-08 Massachusetts Institute Of Technology Methods and compounds for gene insertion into repeated chromosome regions for multi-locus assortment and daisyfield drives
US11920151B2 (en) 2016-09-13 2024-03-05 Toolgen Incorporated Method for identifying DNA base editing by means of cytosine deaminase
WO2018071868A1 (fr) 2016-10-14 2018-04-19 President And Fellows Of Harvard College Administration d'aav d'éditeurs de nucléobases
WO2018119359A1 (fr) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Édition du gène récepteur ccr5 pour protéger contre l'infection par le vih
TW201839136A (zh) 2017-02-06 2018-11-01 瑞士商諾華公司 治療血色素異常症之組合物及方法
WO2018165504A1 (fr) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression de la douleur par édition de gène
WO2018165629A1 (fr) 2017-03-10 2018-09-13 President And Fellows Of Harvard College Éditeur de base cytosine à guanine
IL269458B2 (en) 2017-03-23 2024-02-01 Harvard College Nucleic base editors that include nucleic acid programmable DNA binding proteins
EP3617311A4 (fr) 2017-03-30 2021-04-21 Kyoto University Procédé pour induire un saut d'exon par édition génomique
EP3607071A1 (fr) * 2017-04-06 2020-02-12 DSM IP Assets B.V. Construction d'intégration à guidage automatique (sgic)
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
US11732274B2 (en) 2017-07-28 2023-08-22 President And Fellows Of Harvard College Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE)
WO2019139645A2 (fr) 2017-08-30 2019-07-18 President And Fellows Of Harvard College Éditeurs de bases à haut rendement comprenant une gam
WO2019067011A1 (fr) * 2017-09-29 2019-04-04 Kansas State University Research Foundation Modulation programmée de l'activité crispr/cas9
CN111757937A (zh) 2017-10-16 2020-10-09 布罗德研究所股份有限公司 腺苷碱基编辑器的用途
US20200367479A1 (en) 2017-11-21 2020-11-26 The Regents Of The University Of California Endonuclease sexing and sterilization in insects
CN108285892A (zh) * 2018-02-01 2018-07-17 上海市东方医院 一种稳定产生人内源性Ito，s电流的细胞模型及构建方法与应用
US20210222150A1 (en) * 2018-05-29 2021-07-22 Buck Institute For Research On Aging Gene-drive in dna viruses
GB201810253D0 (en) * 2018-06-22 2018-08-08 Imperial Innovations Ltd Gene drive
MA52107B1 (fr) 2018-07-18 2022-11-30 Plantarcbio Ltd Compositions et procédés pour l'atténuation d'une infestation par le ravageur rhynchophorus ferrugineus
US20210317429A1 (en) 2018-08-20 2021-10-14 The Broad Institute, Inc. Methods and compositions for optochemical control of crispr-cas9
WO2020041384A1 (fr) 2018-08-20 2020-02-27 The Broad Institute, Inc. Dérivés de 3-phényl-2-cyano-azétidine inhibiteurs de l'activité nucléase guidée par l'arn
WO2020041387A1 (fr) 2018-08-20 2020-02-27 The Brigham And Women's Hospital, Inc. Modifications de domaine de dégradation pour la régulation spatio-temporelle de nucléases guidées par arn
US20210177832A1 (en) 2018-08-20 2021-06-17 The Broad Institute, Inc. Inhibitors of rna-guided nuclease target binding and uses thereof
SG11202109882VA (en) 2019-03-19 2021-10-28 Broad Inst Inc Methods and compositions for editing nucleotide sequences
GB201906203D0 (en) * 2019-05-02 2019-06-19 Pirbright Inst Method
AU2020347276A1 (en) 2019-09-12 2022-03-17 Massachusetts Institute Of Technology Engineered adeno-associated virus capsids
JP2023525304A (ja) 2020-05-08 2023-06-15 ザ ブロード インスティテュート，インコーポレーテッド 標的二本鎖ヌクレオチド配列の両鎖同時編集のための方法および組成物
WO2023064706A1 (fr) * 2021-10-12 2023-04-20 The Regents Of The University Of California Système de forçage génétique et sa méthode d'utilisation

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0126251D0 (en) * 2001-11-01 2002-01-02 Imp College Innovations Ltd Methods
WO2011053957A2 (fr) * 2009-11-02 2011-05-05 Gen9, Inc. Compositions et procédés de régulation de multiples gènes d'intérêt dans une cellule
KR101833589B1 (ko) * 2012-02-24 2018-03-02 프레드 헛친슨 켄서 리서치 센터 이상혈색소증 치료를 위한 조성물 및 방법
GB2500113A (en) * 2012-03-05 2013-09-11 Oxitec Ltd Arthropod male germline gene expression system
WO2013141680A1 (fr) * 2012-03-20 2013-09-26 Vilnius University Clivage d'adn dirigé par arn par le complexe cas9-arncr
AU2013259647B2 (en) * 2012-05-07 2018-11-08 Corteva Agriscience Llc Methods and compositions for nuclease-mediated targeted integration of transgenes
DK3401400T3 (da) * 2012-05-25 2019-06-03 Univ California Fremgangsmåder og sammensætninger til rna-styret mål-dna-modifikation og til rna-styret transskriptionsmodulering
US9688971B2 (en) * 2012-06-15 2017-06-27 The Regents Of The University Of California Endoribonuclease and methods of use thereof
CA2877290A1 (fr) * 2012-06-19 2013-12-27 Daniel F. Voytas Ciblage de gene dans des plantes a l'aide de virus a adn
CA2933433C (fr) * 2013-12-11 2020-11-17 Regeneron Pharmaceuticals, Inc. Procedes et compositions pour la modification ciblee d'un genome

Also Published As

Publication number Publication date
EP3092310A4 (fr) 2017-11-22
BR112016015958A2 (pt) 2017-09-19
JP7187508B2 (ja) 2022-12-12
JP2017511685A (ja) 2017-04-27
EP3092310A1 (fr) 2016-11-16
SG11201605550QA (en) 2016-08-30
CA2936312A1 (fr) 2015-07-16
JP2021003112A (ja) 2021-01-14
JP6747974B2 (ja) 2020-08-26
CN106133141A (zh) 2016-11-16
EP3092310B1 (fr) 2019-12-25
US20160333376A1 (en) 2016-11-17
US10526618B2 (en) 2020-01-07
WO2015105928A9 (fr) 2015-09-24
AU2015204784B2 (en) 2021-01-28
AU2021202590A1 (en) 2021-05-27
AU2015204784A1 (en) 2016-07-21
WO2015105928A1 (fr) 2015-07-16
CN106133141B (zh) 2021-08-20
ZA201604874B (en) 2022-08-31

Similar Documents

Publication Publication Date Title
SG10201805815YA (en) Rna-guided gene drives
WO2018129368A3 (fr) Procédés d'évaluation de la coupure par les nucléases
MX2020011939A (es) Nuevas enzimas y sistemas crispr.
WO2019006471A3 (fr) Nouveaux enzymes de ciblage d'arn crispr, systèmes et utilisations associés
AU2020201465A1 (en) Using truncated guide rnas (tru-grnas) to increase specificity for rna-guided genome editing
MX2020007078A (es) Composiciones y metodos para la expresion de arns guias crispr que usan el promotor h1.
MX2017008190A (es) Metodos y composiciones para la modificacion genetica dirigida a traves de direccionamiento multiple de una sola etapa.
WO2016183402A3 (fr) Procédés de fabrication et d'utilisation d'arn de guidage destiné à être utilisé avec des systèmes cas9
EA201991369A1 (ru) Модифицированные направляющие рнк
MX2018001617A (es) Composiciones de crispr-cas9 diseñados y metodos de uso.
GB2569734A (en) RNA-guided nucleic acid modifying enzymes and methods of use thereof
EP3186375A4 (fr) Nouvelles protéines cas9 et éléments de guidage pour le ciblage de l'adn et l'édition du génome
PT3765616T (pt) Novas enzimas e sistemas de direcionamento de dna e rna crispr
NZ727481A (en) Methods and compositions for modifying a targeted locus
EP3845640A3 (fr) Transposases modifiées pour un meilleur biais de séquence d'insertion et une tolérance accrue aux introductions d'adn
WO2016205688A3 (fr) Régulation de la transcription à guidage arn et procédés d'utilisation de celle-ci pour le traitement des lombalgies
WO2015112896A3 (fr) Méthodes et compositions pour des séquences guidant le ciblage de cas9
SG10201809312QA (en) Nucleic acid synthesis techniques
NZ728437A (en) Methods for increasing cas9-mediated engineering efficiency
PH12019500626A1 (en) Aav treatment of huntington's disease
UA118090C2 (uk) Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив
WO2014022702A3 (fr) Procédés et compositions permettant de réguler l'expression génique par maturation de l'arn
NZ705745A (en) Fad3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks
EP3554557A4 (fr) Procédés de traitement de cellules contenant des gènes de fusion par ciblage génomique
SG10201907746TA (en) Methods of treating cancer harboring hemizygous loss of tp53