WO2015112896A3 - Méthodes et compositions pour des séquences guidant le ciblage de cas9 - Google Patents

Méthodes et compositions pour des séquences guidant le ciblage de cas9 Download PDF

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Publication number
WO2015112896A3
WO2015112896A3 PCT/US2015/012747 US2015012747W WO2015112896A3 WO 2015112896 A3 WO2015112896 A3 WO 2015112896A3 US 2015012747 W US2015012747 W US 2015012747W WO 2015112896 A3 WO2015112896 A3 WO 2015112896A3
Authority
WO
WIPO (PCT)
Prior art keywords
compositions
methods
cas9 targeting
sequences
guiding cas9
Prior art date
Application number
PCT/US2015/012747
Other languages
English (en)
Other versions
WO2015112896A9 (fr
WO2015112896A2 (fr
Inventor
Rodolphe Barrangou
Kurt M. SELLE
Alexandra E. BRINER
Original Assignee
North Carolina State University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by North Carolina State University filed Critical North Carolina State University
Priority to US15/113,656 priority Critical patent/US10787654B2/en
Priority to JP2016547162A priority patent/JP2017503514A/ja
Priority to EP15740979.8A priority patent/EP3097212A4/fr
Priority to CA2936646A priority patent/CA2936646C/fr
Publication of WO2015112896A2 publication Critical patent/WO2015112896A2/fr
Publication of WO2015112896A3 publication Critical patent/WO2015112896A3/fr
Publication of WO2015112896A9 publication Critical patent/WO2015112896A9/fr
Priority to US17/002,133 priority patent/US20210017507A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

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  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente invention concerne des méthodes et des compositions pour l'édition du génome et le ciblage de l'ADN de protéines.
PCT/US2015/012747 2014-01-24 2015-01-23 Méthodes et compositions pour des séquences guidant le ciblage de cas9 WO2015112896A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US15/113,656 US10787654B2 (en) 2014-01-24 2015-01-23 Methods and compositions for sequence guiding Cas9 targeting
JP2016547162A JP2017503514A (ja) 2014-01-24 2015-01-23 Ｃａｓ９ターゲッティングをガイドする配列に関する方法および組成物
EP15740979.8A EP3097212A4 (fr) 2014-01-24 2015-01-23 Méthodes et compositions pour des séquences guidant le ciblage de cas9
CA2936646A CA2936646C (fr) 2014-01-24 2015-01-23 Methodes et compositions pour des sequences guidant le ciblage de cas9
US17/002,133 US20210017507A1 (en) 2014-01-24 2020-08-25 Methods and compositions for sequences guiding cas9 targeting

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461931515P 2014-01-24 2014-01-24
US61/931,515 2014-01-24

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US15/113,656 A-371-Of-International US10787654B2 (en) 2014-01-24 2015-01-23 Methods and compositions for sequence guiding Cas9 targeting
US17/002,133 Division US20210017507A1 (en) 2014-01-24 2020-08-25 Methods and compositions for sequences guiding cas9 targeting

Publications (3)

Publication Number Publication Date
WO2015112896A2 WO2015112896A2 (fr) 2015-07-30
WO2015112896A3 true WO2015112896A3 (fr) 2015-10-29
WO2015112896A9 WO2015112896A9 (fr) 2015-11-26

Family

ID=53682132

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2015/012747 WO2015112896A2 (fr) 2014-01-24 2015-01-23 Méthodes et compositions pour des séquences guidant le ciblage de cas9

Country Status (4)

Country Link
EP (1) EP3097212A4 (fr)
JP (4) JP2017503514A (fr)
CA (1) CA2936646C (fr)
WO (1) WO2015112896A2 (fr)

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US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
BR112016003591A8 (pt) 2013-08-22 2018-01-30 Du Pont promotor u6 de polimerase iii de soja e métodos de uso
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
WO2015066119A1 (fr) 2013-10-30 2015-05-07 North Carolina State University Compositions et méthodes liées à un système crispr-cas de type ii chez lactobacillus buchneri
WO2015070083A1 (fr) 2013-11-07 2015-05-14 Editas Medicine,Inc. Méthodes et compositions associées à crispr avec arng de régulation
US9068179B1 (en) 2013-12-12 2015-06-30 President And Fellows Of Harvard College Methods for correcting presenilin point mutations
EP3097212A4 (fr) * 2014-01-24 2017-10-04 North Carolina State University Méthodes et compositions pour des séquences guidant le ciblage de cas9
US11439712B2 (en) 2014-04-08 2022-09-13 North Carolina State University Methods and compositions for RNA-directed repression of transcription using CRISPR-associated genes
AU2015298571B2 (en) 2014-07-30 2020-09-03 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
US10450584B2 (en) 2014-08-28 2019-10-22 North Carolina State University Cas9 proteins and guiding features for DNA targeting and genome editing
EP3274460A1 (fr) 2015-03-27 2018-01-31 E. I. du Pont de Nemours and Company Promoteurs de gène d'arn nucléaire de petite taille u6 de soja et leur utilisation dans l'expression constitutive de gènes d'arn de petite taille dans des plantes
AU2016265560B2 (en) * 2015-05-15 2022-07-28 Pioneer Hi-Bred International, Inc. Rapid characterization of Cas endonuclease systems, PAM sequences and guide RNA elements
CN108026536A (zh) 2015-05-29 2018-05-11 北卡罗来纳州立大学 使用crispr核酸筛选细菌、古细菌、藻类和酵母的方法
EP4299754A3 (fr) 2015-06-15 2024-03-20 North Carolina State University Procédés et compositions permettant une délivrance efficace d'acides nucléiques et d'antimicrobiens à base d'arn
US9580727B1 (en) 2015-08-07 2017-02-28 Caribou Biosciences, Inc. Compositions and methods of engineered CRISPR-Cas9 systems using split-nexus Cas9-associated polynucleotides
EP3356533A1 (fr) 2015-09-28 2018-08-08 North Carolina State University Méthodes et compositions pour agents antimicrobiens spécifiques d'une séquence
WO2017070632A2 (fr) 2015-10-23 2017-04-27 President And Fellows Of Harvard College Éditeurs de nucléobases et leurs utilisations
WO2017112620A1 (fr) 2015-12-22 2017-06-29 North Carolina State University Méthodes et compositions pour l'administration d'agents antimicrobiens à base de crispr
CN105594664B (zh) * 2016-02-16 2018-10-02 湖南师范大学 一种基因敲除选育stat1a基因缺失型斑马鱼的方法
CA3032699A1 (fr) 2016-08-03 2018-02-08 President And Fellows Of Harvard College Editeurs de nucleobases d'adenosine et utilisations associees
WO2018031683A1 (fr) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Protéines de fusion cas9-recombinase programmables et utilisations associées
WO2018039438A1 (fr) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation d'acides aminés non naturels dans des protéines au moyen de l'édition de bases
EP3526320A1 (fr) 2016-10-14 2019-08-21 President and Fellows of Harvard College Administration d'aav d'éditeurs de nucléobases
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
TW201839136A (zh) 2017-02-06 2018-11-01 瑞士商諾華公司 治療血色素異常症之組合物及方法
EP3592853A1 (fr) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression de la douleur par édition de gène
JP2020510439A (ja) 2017-03-10 2020-04-09 プレジデント アンド フェローズ オブ ハーバード カレッジ シトシンからグアニンへの塩基編集因子
KR102687373B1 (ko) 2017-03-23 2024-07-23 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
WO2018209320A1 (fr) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Arn guides incorporés par aptazyme pour une utilisation avec crispr-cas9 dans l'édition du génome et l'activation transcriptionnelle
EP3630975A4 (fr) * 2017-05-26 2021-03-10 North Carolina State University Arn guides modifiés pour moduler l'activité cas9 et procédés d'utilisation
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EP3658573A1 (fr) 2017-07-28 2020-06-03 President and Fellows of Harvard College Procédés et compositions pour l'évolution d'éditeurs de bases à l'aide d'une évolution continue assistée par phage (pace)
WO2019139645A2 (fr) 2017-08-30 2019-07-18 President And Fellows Of Harvard College Éditeurs de bases à haut rendement comprenant une gam
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WO2020191249A1 (fr) 2019-03-19 2020-09-24 The Broad Institute, Inc. Procédés et compositions pour l'édition de séquences nucléotidiques
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WO2020263773A1 (fr) * 2019-06-24 2020-12-30 North Carolina State University Procédés et compositions pour modifier des génomes bactériens et pour tuer des bactéries
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MX2022014008A (es) 2020-05-08 2023-02-09 Broad Inst Inc Métodos y composiciones para la edición simultánea de ambas cadenas de una secuencia de nucleótidos de doble cadena objetivo.

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Also Published As

Publication number Publication date
JP2024041934A (ja) 2024-03-27
EP3097212A2 (fr) 2016-11-30
JP7429057B2 (ja) 2024-02-07
CA2936646C (fr) 2024-04-30
EP3097212A4 (fr) 2017-10-04
WO2015112896A9 (fr) 2015-11-26
WO2015112896A2 (fr) 2015-07-30
CA2936646A1 (fr) 2015-07-30
JP2022106883A (ja) 2022-07-20
JP2020103295A (ja) 2020-07-09
JP2017503514A (ja) 2017-02-02

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