EP3097212A4 - Méthodes et compositions pour des séquences guidant le ciblage de cas9 - Google Patents
Méthodes et compositions pour des séquences guidant le ciblage de cas9 Download PDFInfo
- Publication number
- EP3097212A4 EP3097212A4 EP15740979.8A EP15740979A EP3097212A4 EP 3097212 A4 EP3097212 A4 EP 3097212A4 EP 15740979 A EP15740979 A EP 15740979A EP 3097212 A4 EP3097212 A4 EP 3097212A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- compositions
- methods
- cas9 targeting
- sequences
- guiding cas9
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201461931515P | 2014-01-24 | 2014-01-24 | |
PCT/US2015/012747 WO2015112896A2 (fr) | 2014-01-24 | 2015-01-23 | Méthodes et compositions pour des séquences guidant le ciblage de cas9 |
Publications (2)
Publication Number | Publication Date |
---|---|
EP3097212A2 EP3097212A2 (fr) | 2016-11-30 |
EP3097212A4 true EP3097212A4 (fr) | 2017-10-04 |
Family
ID=53682132
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP15740979.8A Pending EP3097212A4 (fr) | 2014-01-24 | 2015-01-23 | Méthodes et compositions pour des séquences guidant le ciblage de cas9 |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP3097212A4 (fr) |
JP (4) | JP2017503514A (fr) |
CA (1) | CA2936646A1 (fr) |
WO (1) | WO2015112896A2 (fr) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110117622A (zh) * | 2019-05-24 | 2019-08-13 | 青岛农业大学 | 一种CRISPR/Cas基因编辑系统及其制备方法和应用 |
Families Citing this family (45)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP6261500B2 (ja) | 2011-07-22 | 2018-01-17 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ヌクレアーゼ切断特異性の評価および改善 |
US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
LT3036327T (lt) | 2013-08-22 | 2019-06-25 | Pioneer Hi-Bred International, Inc. | Genomo modifikacijos, panaudojant nukreipiančias polinukleotido/cas endonukleazės sistemas, ir panaudojimo būdai |
US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
US9340800B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | Extended DNA-sensing GRNAS |
US10584358B2 (en) | 2013-10-30 | 2020-03-10 | North Carolina State University | Compositions and methods related to a type-II CRISPR-Cas system in Lactobacillus buchneri |
AU2014346559B2 (en) | 2013-11-07 | 2020-07-09 | Editas Medicine,Inc. | CRISPR-related methods and compositions with governing gRNAs |
US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
WO2015112896A2 (fr) * | 2014-01-24 | 2015-07-30 | North Carolina State University | Méthodes et compositions pour des séquences guidant le ciblage de cas9 |
JP2017512481A (ja) | 2014-04-08 | 2017-05-25 | ノースカロライナ ステート ユニバーシティーNorth Carolina State University | Crispr関連遺伝子を用いた、rna依存性の転写抑制のための方法および組成物 |
US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
EP3633032A3 (fr) | 2014-08-28 | 2020-07-29 | North Carolina State University | Nouvelles protéines cas9 et éléments de guidage pour le ciblage de l'adn et l'édition du génome |
CN107567499A (zh) | 2015-03-27 | 2018-01-09 | 纳幕尔杜邦公司 | 大豆u6核小rna基因启动子及其在植物小rna基因的组成型表达中的用途 |
AU2016265560B2 (en) * | 2015-05-15 | 2022-07-28 | Pioneer Hi-Bred International, Inc. | Rapid characterization of Cas endonuclease systems, PAM sequences and guide RNA elements |
US10136649B2 (en) | 2015-05-29 | 2018-11-27 | North Carolina State University | Methods for screening bacteria, archaea, algae, and yeast using CRISPR nucleic acids |
FI3307872T3 (fi) | 2015-06-15 | 2023-10-16 | Univ North Carolina State | Menetelmiä ja koostumuksia nukleiinihappojen ja rna-pohjaisten antimikrobien tehokkaaseen antamiseen |
US9580727B1 (en) | 2015-08-07 | 2017-02-28 | Caribou Biosciences, Inc. | Compositions and methods of engineered CRISPR-Cas9 systems using split-nexus Cas9-associated polynucleotides |
WO2017058751A1 (fr) | 2015-09-28 | 2017-04-06 | North Carolina State University | Méthodes et compositions pour agents antimicrobiens spécifiques d'une séquence |
WO2017070632A2 (fr) | 2015-10-23 | 2017-04-27 | President And Fellows Of Harvard College | Éditeurs de nucléobases et leurs utilisations |
US11542466B2 (en) | 2015-12-22 | 2023-01-03 | North Carolina State University | Methods and compositions for delivery of CRISPR based antimicrobials |
CN105594664B (zh) * | 2016-02-16 | 2018-10-02 | 湖南师范大学 | 一种基因敲除选育stat1a基因缺失型斑马鱼的方法 |
WO2018027078A1 (fr) | 2016-08-03 | 2018-02-08 | President And Fellows Of Harard College | Éditeurs de nucléobases d'adénosine et utilisations associées |
CA3033327A1 (fr) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Proteines de fusion cas9-recombinase programmables et utilisations associees |
WO2018039438A1 (fr) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation d'acides aminés non naturels dans des protéines au moyen de l'édition de bases |
GB2573062A (en) | 2016-10-14 | 2019-10-23 | Harvard College | AAV delivery of nucleobase editors |
WO2018119359A1 (fr) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Édition du gène récepteur ccr5 pour protéger contre l'infection par le vih |
TW201839136A (zh) | 2017-02-06 | 2018-11-01 | 瑞士商諾華公司 | 治療血色素異常症之組合物及方法 |
US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
WO2018165629A1 (fr) | 2017-03-10 | 2018-09-13 | President And Fellows Of Harvard College | Éditeur de base cytosine à guanine |
KR20190130613A (ko) | 2017-03-23 | 2019-11-22 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
WO2018217981A1 (fr) | 2017-05-26 | 2018-11-29 | North Carolina State University | Arn guides modifiés pour moduler l'activité cas9 et procédés d'utilisation |
US11866726B2 (en) | 2017-07-14 | 2024-01-09 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
WO2019139645A2 (fr) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | Éditeurs de bases à haut rendement comprenant une gam |
WO2019079347A1 (fr) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | Utilisations d'éditeurs de bases adénosine |
EP3755792A4 (fr) * | 2018-02-23 | 2021-12-08 | Pioneer Hi-Bred International, Inc. | Nouveaux orthologues de cas9 |
US10711267B2 (en) | 2018-10-01 | 2020-07-14 | North Carolina State University | Recombinant type I CRISPR-Cas system |
BR112021011372A2 (pt) | 2018-12-14 | 2021-08-31 | Pioneer Hi-Bred International, Inc. | Novos sistemas crispr-cas para edição de genoma |
KR20210143230A (ko) | 2019-03-19 | 2021-11-26 | 더 브로드 인스티튜트, 인코퍼레이티드 | 뉴클레오티드 서열을 편집하기 위한 방법 및 조성물 |
WO2020263773A1 (fr) * | 2019-06-24 | 2020-12-30 | North Carolina State University | Procédés et compositions pour modifier des génomes bactériens et pour tuer des bactéries |
US20230332218A1 (en) * | 2020-04-21 | 2023-10-19 | Mammoth Biosciences, Inc. | Casy programmable nucleases and rna component systems |
CN116096873A (zh) | 2020-05-08 | 2023-05-09 | 布罗德研究所股份有限公司 | 同时编辑靶标双链核苷酸序列的两条链的方法和组合物 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013176772A1 (fr) * | 2012-05-25 | 2013-11-28 | The Regents Of The University Of California | Procédés et compositions permettant la modification de l'adn cible dirigée par l'arn et la modulation de la transcription dirigée par l'arn |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2015112896A2 (fr) * | 2014-01-24 | 2015-07-30 | North Carolina State University | Méthodes et compositions pour des séquences guidant le ciblage de cas9 |
-
2015
- 2015-01-23 WO PCT/US2015/012747 patent/WO2015112896A2/fr active Application Filing
- 2015-01-23 JP JP2016547162A patent/JP2017503514A/ja active Pending
- 2015-01-23 CA CA2936646A patent/CA2936646A1/fr active Pending
- 2015-01-23 EP EP15740979.8A patent/EP3097212A4/fr active Pending
-
2020
- 2020-02-13 JP JP2020022160A patent/JP2020103295A/ja active Pending
-
2022
- 2022-04-28 JP JP2022074741A patent/JP7429057B2/ja active Active
-
2024
- 2024-01-16 JP JP2024004564A patent/JP2024041934A/ja active Pending
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013176772A1 (fr) * | 2012-05-25 | 2013-11-28 | The Regents Of The University Of California | Procédés et compositions permettant la modification de l'adn cible dirigée par l'arn et la modulation de la transcription dirigée par l'arn |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110117622A (zh) * | 2019-05-24 | 2019-08-13 | 青岛农业大学 | 一种CRISPR/Cas基因编辑系统及其制备方法和应用 |
Also Published As
Publication number | Publication date |
---|---|
JP2024041934A (ja) | 2024-03-27 |
JP7429057B2 (ja) | 2024-02-07 |
EP3097212A2 (fr) | 2016-11-30 |
CA2936646A1 (fr) | 2015-07-30 |
WO2015112896A2 (fr) | 2015-07-30 |
JP2017503514A (ja) | 2017-02-02 |
JP2022106883A (ja) | 2022-07-20 |
WO2015112896A9 (fr) | 2015-11-26 |
JP2020103295A (ja) | 2020-07-09 |
WO2015112896A3 (fr) | 2015-10-29 |
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Legal Events
Date | Code | Title | Description |
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PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
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17P | Request for examination filed |
Effective date: 20160824 |
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AK | Designated contracting states |
Kind code of ref document: A2 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
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AX | Request for extension of the european patent |
Extension state: BA ME |
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RIN1 | Information on inventor provided before grant (corrected) |
Inventor name: SELLE, KURT M. Inventor name: BARRANGOU, RODOLPHE Inventor name: BRINER, ALEXANDRA E. |
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DAX | Request for extension of the european patent (deleted) | ||
A4 | Supplementary search report drawn up and despatched |
Effective date: 20170831 |
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RIC1 | Information provided on ipc code assigned before grant |
Ipc: C12N 15/85 20060101ALI20170825BHEP Ipc: C12N 15/113 20100101ALI20170825BHEP Ipc: C12N 15/00 20060101ALI20170825BHEP Ipc: C07H 21/04 20060101ALI20170825BHEP Ipc: A61K 48/00 20060101ALI20170825BHEP Ipc: C12P 19/34 20060101ALI20170825BHEP Ipc: C12Q 1/68 20060101AFI20170825BHEP Ipc: C12N 9/16 20060101ALI20170825BHEP |
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STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: EXAMINATION IS IN PROGRESS |
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17Q | First examination report despatched |
Effective date: 20181030 |
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STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: EXAMINATION IS IN PROGRESS |
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STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: EXAMINATION IS IN PROGRESS |