SG10201604400RA - Micro-incubation systems for microfluidic cell culture and methods - Google Patents

Micro-incubation systems for microfluidic cell culture and methods

Info

Publication number
SG10201604400RA
SG10201604400RA SG10201604400RA SG10201604400RA SG10201604400RA SG 10201604400R A SG10201604400R A SG 10201604400RA SG 10201604400R A SG10201604400R A SG 10201604400RA SG 10201604400R A SG10201604400R A SG 10201604400RA SG 10201604400R A SG10201604400R A SG 10201604400RA
Authority
SG
Singapore
Prior art keywords
micro
methods
cell culture
microfluidic cell
incubation systems
Prior art date
Application number
SG10201604400RA
Other languages
English (en)
Inventor
Philip J Lee
Terry Gaige
Jessie Ho
Original Assignee
Emd Millipore Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Emd Millipore Corp filed Critical Emd Millipore Corp
Publication of SG10201604400RA publication Critical patent/SG10201604400RA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/56—Labware specially adapted for transferring fluids
    • B01L3/563—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/36—Means for collection or storage of gas; Gas holders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/40—Manifolds; Distribution pieces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/24—Recirculation of gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14—Incubators; Climatic chambers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B01L2200/146—Employing pressure sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B01L2200/147—Employing temperature sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/046—Function or devices integrated in the closure
    • B01L2300/048—Function or devices integrated in the closure enabling gas exchange, e.g. vents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/14—Means for pressure control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1894—Cooling means; Cryo cooling
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Dispersion Chemistry (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Thermal Sciences (AREA)
  • Cell Biology (AREA)
  • Fluid Mechanics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Computer Hardware Design (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG10201604400RA 2011-12-03 2012-12-03 Micro-incubation systems for microfluidic cell culture and methods SG10201604400RA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201161566651P 2011-12-03 2011-12-03

Publications (1)

Publication Number Publication Date
SG10201604400RA true SG10201604400RA (en) 2016-07-28

Family

ID=48536170

Family Applications (3)

Application Number Title Priority Date Filing Date
SG11201402558QA SG11201402558QA (en) 2011-12-03 2012-12-03 Micro-incubation systems for microfluidic cell culture and methods
SG10201604400RA SG10201604400RA (en) 2011-12-03 2012-12-03 Micro-incubation systems for microfluidic cell culture and methods
SG10201609393QA SG10201609393QA (en) 2011-12-03 2012-12-03 Micro-incubation systems for microfluidic cell culture and methods

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG11201402558QA SG11201402558QA (en) 2011-12-03 2012-12-03 Micro-incubation systems for microfluidic cell culture and methods

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG10201609393QA SG10201609393QA (en) 2011-12-03 2012-12-03 Micro-incubation systems for microfluidic cell culture and methods

Country Status (7)

Country Link
US (3) US9206384B2 (ja)
EP (1) EP2785825B1 (ja)
JP (4) JP6232383B2 (ja)
CN (3) CN107345204B (ja)
ES (1) ES2870857T3 (ja)
SG (3) SG11201402558QA (ja)
WO (1) WO2013082612A1 (ja)

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9354156B2 (en) 2007-02-08 2016-05-31 Emd Millipore Corporation Microfluidic particle analysis method, device and system
US9637715B2 (en) 2005-07-07 2017-05-02 Emd Millipore Corporation Cell culture and invasion assay method and system
US9388374B2 (en) 2005-07-07 2016-07-12 Emd Millipore Corporation Microfluidic cell culture systems
US8257964B2 (en) 2006-01-04 2012-09-04 Cell ASIC Microwell cell-culture device and fabrication method
EP2245453B1 (en) 2008-01-03 2016-10-05 EMD Millipore Corporation Microfluidic cell culture array system for automated assays and methods of operation
US9353342B2 (en) 2010-01-21 2016-05-31 Emd Millipore Corporation Cell culture and gradient migration assay methods and devices
US10526572B2 (en) 2011-04-01 2020-01-07 EMD Millipore Corporaticn Cell culture and invasion assay method and system
SG11201402558QA (en) 2011-12-03 2014-06-27 Emd Millipore Corp Micro-incubation systems for microfluidic cell culture and methods
US10921313B2 (en) * 2012-07-16 2021-02-16 Micobiomed., Ltd Point of care testing assay system using novel microfluidics
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2900543C (en) 2013-02-08 2023-01-31 10X Genomics, Inc. Partitioning and processing of analytes and other species
WO2014190258A1 (en) * 2013-05-23 2014-11-27 The Regents Of The University Of California Proximal degas driven microfluidic actuation
CA3209478A1 (en) 2013-07-12 2015-01-15 President And Fellows Of Harvard College Systems and methods for cell culture device interconnection and fluidic device interconnection
CN103667054B (zh) * 2013-09-18 2015-09-30 中国航天员科研训练中心 一种集成化微流控细胞培养芯片及其制备方法
CA2927947A1 (en) * 2013-10-22 2015-04-30 Cellanyx Diagnostics, Llc Systems, devices and methods for microfluidic culturing, manipulation and analysis of tissues and cells
AU2015249274B2 (en) 2014-04-24 2021-07-22 Rapid Micro Biosystems, Inc. Microbiological growth media and methods of using the same
EP4053292A1 (en) 2014-06-26 2022-09-07 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
GB2546424A (en) * 2014-07-14 2017-07-19 Harvard College Systems and methods for improved performance of fluidic and microfluidic systems
US20170247652A1 (en) * 2014-10-20 2017-08-31 Northeastern University Device and Method for High Throughput Bacterial Isolation
BR112017014902A2 (pt) 2015-01-12 2018-03-13 10X Genomics Inc processos e sistemas para a preparação de bibliotecas de sequenciamento de ácido nucleico e bibliotecas preparadas usando os mesmos
US9861982B2 (en) 2015-03-09 2018-01-09 Emd Millipore Corporation Connectors for pneumatic devices in microfluidic systems
WO2016161163A2 (en) * 2015-03-31 2016-10-06 Thrive Bioscience, Inc. Cell culture incubators with integrated imaging systems
WO2016161169A2 (en) 2015-03-31 2016-10-06 Thrive Bioscience, Inc. Cell culture incubators with integrated cell manipulation systems
CN105013551B (zh) * 2015-08-18 2017-01-25 苏州大学 一种智能移液器支架
AU2016312678B2 (en) 2015-08-26 2019-12-12 EMULATE, Inc. Perfusion manifold assembly
US20190262469A1 (en) * 2015-09-04 2019-08-29 C. Jeffrey Brinker Protocells to treat microbial infection and for synergistic delivery
CN108367290B (zh) * 2015-10-01 2021-06-04 伯克利之光生命科技公司 井孔板培养器
JP6912112B2 (ja) * 2015-10-05 2021-07-28 株式会社タカゾノテクノロジー 微生物検出装置
KR102379308B1 (ko) * 2015-11-04 2022-03-28 닛토덴코 가부시키가이샤 생체유체 샘플 분배 및/또는 분석을 위한 장치 및 시스템
US11672866B2 (en) 2016-01-08 2023-06-13 Paul N. DURFEE Osteotropic nanoparticles for prevention or treatment of bone metastases
TWI728237B (zh) * 2016-01-28 2021-05-21 日商精工愛普生股份有限公司 電子零件搬送裝置及電子零件檢查裝置
WO2017151098A1 (en) 2016-02-29 2017-09-08 Hewlett-Packard Development Company, L.P. Liquid directing sample container
CN109996863A (zh) 2016-11-24 2019-07-09 阿尔维欧里克斯股份公司 细胞培养系统和方法
JP6826200B2 (ja) * 2017-01-19 2021-02-03 エッセン インストゥルメンツ，インコーポレイテッド ディー／ビー／エー エッセン バイオサイエンス，インコーポレイテッド マイクロプレート実験器具の灌流および環境制御のための方法および装置
WO2018160865A1 (en) 2017-03-01 2018-09-07 Charles Jeffrey Brinker Active targeting of cells by monosized protocells
US10641733B2 (en) * 2017-03-20 2020-05-05 National Technology & Engineering Solutions Of Sandia, Llc Active mechanical-environmental-thermal MEMS device for nanoscale characterization
WO2018213357A1 (en) * 2017-05-16 2018-11-22 Cairn Biosciences, Inc. Microfluidic-enabled multiwell cell culture devices and systems for precision culture, control and monitoring of living cells
WO2018226546A1 (en) 2017-06-05 2018-12-13 10X Genomics, Inc. Gaskets for the distribution of pressures in a microfluidic system
US11649447B2 (en) 2017-06-12 2023-05-16 Duke University Compositions, systems, and methods for the production of biomolecules
EP3427829A1 (en) * 2017-07-12 2019-01-16 Lunaphore Technologies SA Methods of in situ antigen retrieval of a biological sample & imaging thereof
JP6948914B2 (ja) 2017-10-30 2021-10-13 アークレイ株式会社 分析装置
WO2019114996A1 (de) * 2017-12-15 2019-06-20 Technische Universität Ilmenau Mikrobioreaktoranordnung
CN109385374B (zh) * 2018-10-19 2022-04-15 东南大学 一种微型细胞培养装置及其腔体内部环境控制方法
CN113874519A (zh) * 2019-03-11 2021-12-31 通用自动化实验技术公司 在高密度生长平台上选择微生物分离物的方法
DE102019003444A1 (de) 2019-05-16 2020-11-19 Evorion Biotechnologies Gmbh Inkubationssystem
WO2020247671A1 (en) * 2019-06-05 2020-12-10 Amgen Inc. Systems for performing cellular analysis and related devices for conditioning environments adjacent chips in such systems
US20210008550A1 (en) * 2019-07-09 2021-01-14 Kryptos Biotechnologies, Inc. Microfluidic reaction vessel array with patterned films
NL2024202B1 (en) * 2019-11-08 2021-07-20 Mimetas B V Microfluidic cell culture system
CN114829574A (zh) * 2019-11-19 2022-07-29 何慧君 细胞培养系统及使用其的方法
US11136543B1 (en) 2020-02-11 2021-10-05 Edward R. Flynn Magnetic cell incubation device
IT202000014815A1 (it) * 2020-06-19 2021-12-19 Univ Degli Studi Magna Graecia Di Catanzaro Dispositivo microfuidico per colture e screening cellulari e relativo metodo di produzione
EP3978596A1 (en) 2020-10-02 2022-04-06 Sorbonne Universite Device for providing a liquid medium with a controlled flow rate and with a controlled dissolved gas concentration
EP4137558A1 (en) * 2021-08-17 2023-02-22 Nagi Bioscience SA Apparatus for microscopic worm observation
EP4194542A1 (en) * 2021-12-07 2023-06-14 Fluigent Apparatus for feeding a liquid medium to a fluidic system
CN115322899B (zh) * 2022-06-07 2024-05-24 济宁医学院 一种细胞生物学实验用细胞培养装置

Family Cites Families (179)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1888208A (en) 1932-03-29 1932-11-22 David Luptons Sons Co Metallic furniture
US1985708A (en) 1932-08-02 1934-12-25 Oscar U Zerk Shackle mechanism
US3729708A (en) 1971-10-27 1973-04-24 Eastman Kodak Co Error detecting and correcting apparatus for use in a system wherein phase encoded binary information is recorded on a plural track
US4051204A (en) * 1973-12-21 1977-09-27 Hans Muller Apparatus for mixing a liquid phase and a gaseous phase
US4055613A (en) 1974-10-23 1977-10-25 Akrosil Corporation Production of three-dimensional designs
GB1539263A (en) 1976-11-19 1979-01-31 Toray Industries Apparatus and methods for growing cells
DE3409501A1 (de) 1984-03-15 1985-10-24 Sandoz-Patent-GmbH, 7850 Lörrach Verfahren zur kultivierung von zellen
US4748124A (en) 1984-10-30 1988-05-31 E. I. Du Pont De Nemours And Company Compartmentalized cell-culture device and method
US4661455A (en) 1986-01-16 1987-04-28 Dorr-Oliver Incorporated Membrane cell culturing device
US4734373A (en) 1986-06-24 1988-03-29 Bartal Arie H Apparatus for enhancing cell growth, preservation and transport
US5786215A (en) 1987-05-20 1998-07-28 Baxter International Inc. Method for culturing animal cells
US5153131A (en) 1990-12-11 1992-10-06 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration High aspect reactor vessel and method of use
US5416022A (en) 1988-08-10 1995-05-16 Cellex Biosciences, Inc. Cell culture apparatus
US5079168A (en) 1988-08-10 1992-01-07 Endotronics, Inc. Cell culture apparatus
DE69013764T2 (de) 1989-06-03 1995-03-30 Kanegafuchi Chemical Ind Kontrolle der Zellanordnung.
FR2660323B1 (fr) 1990-03-30 1992-07-24 Bertin & Cie Dispositif de culture cellulaire.
SE470347B (sv) 1990-05-10 1994-01-31 Pharmacia Lkb Biotech Mikrostruktur för vätskeflödessystem och förfarande för tillverkning av ett sådant system
US5310676A (en) 1991-11-15 1994-05-10 A/S Nunc Cell cultivating device
DE4206585C2 (de) 1992-03-03 1994-11-24 Augustinus Dr Med Bader Vorrichtung zur Massenkultur von Zellen
US5637469A (en) 1992-05-01 1997-06-10 Trustees Of The University Of Pennsylvania Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems
US5330908A (en) 1992-12-23 1994-07-19 The United States Of America As Represented By The Administrator, National Aeronautics And Space Administration High density cell culture system
US5424209A (en) 1993-03-19 1995-06-13 Kearney; George P. Automated cell culture and testing system
US5462874A (en) 1993-06-23 1995-10-31 Wolf; Martin L. Dialyzed multiple well tissue culture plate
US5686301A (en) 1993-09-02 1997-11-11 Heraeus Instruments Gmbh Culture vessel for cell cultures
US5702941A (en) 1993-09-09 1997-12-30 Synthecon, Inc. Gas permeable bioreactor and method of use
US5437998A (en) 1993-09-09 1995-08-01 Synthecon, Inc. Gas permeable bioreactor and method of use
US5763279A (en) 1993-09-09 1998-06-09 Synthecon, Inc. Gas permeable bioreactor and method of use
US5451524A (en) 1994-02-01 1995-09-19 The Gillette Company In vitro chamber for human organ tissue samples
US5882918A (en) 1995-08-08 1999-03-16 Genespan Corporation Cell culture incubator
US5793440A (en) 1994-03-18 1998-08-11 Sony Corporation Key signal processing apparatus for video signal processing
US5565353A (en) 1994-06-22 1996-10-15 Board Of Regents, The University Of Texas System Perfusable culture device
US5693537A (en) 1994-06-28 1997-12-02 Wilson; John R. Compartmentalized tissue culture flask
US5714384A (en) 1994-06-28 1998-02-03 Wilson; John R. Compartmentalized tissue culture bag
GB9413029D0 (en) 1994-06-29 1994-08-17 Common Services Agency Stem cell immobilisation
US6297046B1 (en) 1994-10-28 2001-10-02 Baxter International Inc. Multilayer gas-permeable container for the culture of adherent and non-adherent cells
DE19537774C2 (de) 1994-11-18 1996-09-19 Heraeus Instr Gmbh Verfahren zur Zellkultivierung und Vorrichtung zur Durchführung des Verfahrens
US5641644A (en) 1994-12-09 1997-06-24 Board Of Regents, The University Of Texas System Method and apparatus for the precise positioning of cells
EP0725134A3 (en) 1995-02-03 1998-05-13 NPBI Nederlands Produktielaboratorium voor Bloedtransfusieapparatuur en Infusievloeistoffen B.V. Flexible bioreactor for therapeutic cells
CA2175626C (en) 1995-05-18 1999-08-03 Timothy A. Stevens Tissue culture flask
US6096532A (en) 1995-06-07 2000-08-01 Aastrom Biosciences, Inc. Processor apparatus for use in a system for maintaining and growing biological cells
US6228635B1 (en) 1995-06-07 2001-05-08 Aastrom Bioscience, Inc. Portable cell growth cassette for use in maintaining and growing biological cells
US6238908B1 (en) 1995-06-07 2001-05-29 Aastrom Biosciences, Inc. Apparatus and method for maintaining and growth biological cells
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5602028A (en) 1995-06-30 1997-02-11 The University Of British Columbia System for growing multi-layered cell cultures
US5627070A (en) 1995-07-26 1997-05-06 Celltherapy, Inc. Cell growing device for in vitro cell population expansion
US5686304A (en) 1995-12-15 1997-11-11 Avecor Cardiovascular, Inc. Cell culture apparatus and method
JP4071284B2 (ja) 1996-03-05 2008-04-02 シンジェンタ パーティシペーションズ アクチェンゲゼルシャフト 化学物質または化学物質混合物の検査システム
US5942443A (en) 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
CN1173776C (zh) 1996-06-28 2004-11-03 卡钳技术有限公司 在微规模流体性设备里的高通过量的筛选分析系统
US6039897A (en) 1996-08-28 2000-03-21 University Of Washington Multiple patterned structures on a single substrate fabricated by elastomeric micro-molding techniques
US5801054A (en) 1996-09-19 1998-09-01 The United States Of America As Represented By The Secretary Of The Air Force Cell culture vessel with self-maintained atmosphere
EP0988529B1 (en) 1997-04-25 2013-06-12 Caliper Life Sciences, Inc. Microfluidic devices incorporating improved channel geometries
US5932315A (en) 1997-04-30 1999-08-03 Hewlett-Packard Company Microfluidic structure assembly with mating microfeatures
DE19719751A1 (de) 1997-05-10 1998-11-12 Augustinus Dr Med Bader Vorrichtung zum Züchten und/oder Behandeln von Zellen
US6548263B1 (en) 1997-05-29 2003-04-15 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
US7160687B1 (en) 1997-05-29 2007-01-09 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
US6107085A (en) 1997-07-11 2000-08-22 Corning Incorporated Self contained cell growth system
JP3761676B2 (ja) 1997-07-11 2006-03-29 株式会社メニコン 細胞培養用容器
US5876675A (en) 1997-08-05 1999-03-02 Caliper Technologies Corp. Microfluidic devices and systems
US6190913B1 (en) 1997-08-12 2001-02-20 Vijay Singh Method for culturing cells using wave-induced agitation
US6794184B1 (en) 1998-01-19 2004-09-21 Ulrich Mohr Culturing device and method for culturing cells or tissue components
US6857449B1 (en) 1998-01-20 2005-02-22 Caliper Life Sciences, Inc. Multi-layer microfluidic devices
US6167910B1 (en) 1998-01-20 2001-01-02 Caliper Technologies Corp. Multi-layer microfluidic devices
US6756019B1 (en) 1998-02-24 2004-06-29 Caliper Technologies Corp. Microfluidic devices and systems incorporating cover layers
US6251343B1 (en) 1998-02-24 2001-06-26 Caliper Technologies Corp. Microfluidic devices and systems incorporating cover layers
ATE227338T1 (de) 1998-03-18 2002-11-15 Massachusetts Inst Technology Vaskularisierte perfundierte anordnungen für mikrogewebe und mikroorgane
US20040202579A1 (en) 1998-05-08 2004-10-14 Anders Larsson Microfluidic device
US6518035B1 (en) 1998-06-02 2003-02-11 Rosetta Inpharmatics, Inc. Targeted methods of drug screening using co-culture methods
US7155344B1 (en) 1998-07-27 2006-12-26 Caliper Life Sciences, Inc. Distributed database for analytical instruments
GB9817995D0 (en) 1998-08-19 1998-10-14 Griffith John D A surgical sewing device
ATE238412T1 (de) 1998-10-08 2003-05-15 Astrazeneca Ab Mikrofabrizierter zellinjektor
US6637463B1 (en) 1998-10-13 2003-10-28 Biomicro Systems, Inc. Multi-channel microfluidic system design with balanced fluid flow distribution
GB9912641D0 (en) 1999-05-28 1999-07-28 Ashby Scient Ltd Textured and porous silicone rubber
US6548299B1 (en) 1999-11-12 2003-04-15 Mark J. Pykett Lymphoid tissue-specific cell production from hematopoietic progenitor cells in three-dimensional devices
US6613513B1 (en) 1999-02-23 2003-09-02 Caliper Technologies Corp. Sequencing by incorporation
WO2000050871A1 (en) 1999-02-26 2000-08-31 Orchid Biosciences, Inc. Microstructures for use in biological assays and reactions
US6455310B1 (en) 1999-03-23 2002-09-24 Biocrystal Ltd. Cell culture apparatus and method for culturing cells
US6410309B1 (en) 1999-03-23 2002-06-25 Biocrystal Ltd Cell culture apparatus and methods of use
GB9907665D0 (en) 1999-04-01 1999-05-26 Cambridge Molecular Tech Fluidic devices
WO2000078932A1 (en) 1999-06-21 2000-12-28 The General Hospital Corporation Cell culture systems and methods for organ assist devices
WO2000078920A1 (en) 1999-06-21 2000-12-28 The General Hospital Corporation Methods and devices for cell culturing and organ assist systems
TWI233449B (en) 1999-07-01 2005-06-01 Ind Tech Res Inst High efficient cell-cultivating device
US6495104B1 (en) 1999-08-19 2002-12-17 Caliper Technologies Corp. Indicator components for microfluidic systems
US6837985B2 (en) 1999-09-20 2005-01-04 Aeromet Technologies, Inc. External counter electrode
DE19947496C2 (de) 1999-10-01 2003-05-22 Agilent Technologies Inc Mikrofluidischer Mikrochip
DE19948087B4 (de) 1999-10-06 2008-04-17 Evotec Ag Verfahren zur Herstellung eines Reaktionssubstrats
FR2803852B1 (fr) 2000-01-17 2004-11-05 Farzin Sarem Dispositif de culture cellulaire et tissulaire a circulation de fluide de culture controlee
AU4907101A (en) 2000-02-23 2001-09-03 Caliper Technologies Inc Multi-reservoir pressure control system
US6593136B1 (en) 2000-03-20 2003-07-15 Geo-Centers, Inc. Culturing cells in a chamber in a stack of chambers
US7867763B2 (en) 2004-01-25 2011-01-11 Fluidigm Corporation Integrated chip carriers with thermocycler interfaces and methods of using the same
US7323143B2 (en) 2000-05-25 2008-01-29 President And Fellows Of Harvard College Microfluidic systems including three-dimensionally arrayed channel networks
US6569675B2 (en) 2000-06-16 2003-05-27 Corning Incorporated Cell cultivating flask and method for using the cell cultivating flask
DE60013585T2 (de) 2000-07-19 2005-09-15 Technodop Ltd. (Société de Droit Irlandais) Kulturraum und Bioreaktor zur ausserkörperlichen Tierzellenkultur
JP4273643B2 (ja) 2000-08-08 2009-06-03 味の素株式会社 好気性培養における培養装置及び消泡制御方法
DE10046175A1 (de) 2000-09-19 2002-03-28 Augustinus Bader Verfahren und Vorrichtung zum Züchten und/oder Behandeln von Zellen
US20020039785A1 (en) 2000-10-04 2002-04-04 Schroeder Kirk S. Cell-culture vessel
AU2002216618A1 (en) 2000-10-06 2002-04-15 Protasis Corporation Fluid separation conduit cartridge with encryption capability
US7514046B2 (en) 2000-10-31 2009-04-07 Caliper Life Sciences, Inc. Methods and systems for processing microscale devices for reuse
US7211209B2 (en) 2000-11-08 2007-05-01 Surface Logix, Inc. Method of making device for arraying biomolecules and for monitoring cell motility in real-time
US6653124B1 (en) 2000-11-10 2003-11-25 Cytoplex Biosciences Inc. Array-based microenvironment for cell culturing, cell monitoring and drug-target validation
US6555365B2 (en) 2000-12-07 2003-04-29 Biocrystal, Ltd. Microincubator comprising a cell culture apparatus and a transparent heater
US20020108860A1 (en) 2001-01-15 2002-08-15 Staats Sau Lan Tang Fabrication of polymeric microfluidic devices
US6403369B1 (en) 2001-01-19 2002-06-11 Gary W. Wood Cell culture vessel
US20020110905A1 (en) 2001-02-15 2002-08-15 Emilio Barbera-Guillem Perfusion system for cultured cells
EP1248108B1 (en) 2001-03-29 2011-08-31 Canon Kabushiki Kaisha method of cellular screening and substrates suitable for it
AU2002307152A1 (en) 2001-04-06 2002-10-21 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
US6811752B2 (en) 2001-05-15 2004-11-02 Biocrystal, Ltd. Device having microchambers and microfluidics
DE60125598T2 (de) 2001-06-08 2007-10-04 Centre National De La Recherche Scientifique Methode zur herstellung einer mikroflüssigkeitsstruktur, insbesondere eines "biochips", und damit erzeugte struktur
US6670171B2 (en) 2001-07-09 2003-12-30 Wheaton Usa, Inc. Disposable vessel
US6673595B2 (en) 2001-08-27 2004-01-06 Biocrystal, Ltd Automated cell management system for growth and manipulation of cultured cells
CA2468674A1 (en) 2001-12-05 2003-06-12 University Of Washington Microfluidic device and surface decoration process for solid phase affinity binding assays
CA2471706C (en) 2001-12-21 2011-10-18 Organogenesis Inc. Chamber with adjustable volume for cell culture and organ assist
US7033823B2 (en) 2002-01-31 2006-04-25 Cesco Bioengineering, Inc. Cell-cultivating device
EP1490520A4 (en) 2002-03-12 2006-06-07 Surface Logix Inc TEST APPARATUS ANALYZING THE ABSORPTION, METABOLISM, PERMEABILITY AND / OR TOXICITY OF A CANDIDATE COMPOUND
US7223371B2 (en) 2002-03-14 2007-05-29 Micronics, Inc. Microfluidic channel network device
JP4554216B2 (ja) 2002-03-31 2010-09-29 ユィロス・パテント・アクチボラグ 効率的なマイクロ流体デバイス
US7312085B2 (en) 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
JP2005521425A (ja) 2002-04-01 2005-07-21 フルイディグム コーポレイション 微小流体粒子分析システム
AU2002246422A1 (en) 2002-04-04 2003-10-20 Corebiotech Co., Ltd. Cell culture plate and system using the same
US8492140B2 (en) * 2002-04-08 2013-07-23 Octane Biotech Inc. Automated tissue engineering system
US20060199260A1 (en) 2002-05-01 2006-09-07 Zhiyu Zhang Microbioreactor for continuous cell culture
US7229820B2 (en) 2002-06-13 2007-06-12 Wilson Wolf Manufacturing Corporation Apparatus and method for culturing and preserving tissue constructs
AU2003251874A1 (en) 2002-07-12 2004-02-02 Dirk R. Albrecht Three dimensional cell patterned bioploymer scaffolds and method of making the same
US20040029266A1 (en) 2002-08-09 2004-02-12 Emilio Barbera-Guillem Cell and tissue culture device
EP1546299A4 (en) 2002-08-27 2007-07-04 Univ Vanderbilt CAPILLARY-FOUNDED BIOREACTORS WITH MULTIPLE CHAMBERS
US7790443B2 (en) 2002-08-27 2010-09-07 Vanderbilt University Bioreactors with substance injection capacity
US8147562B2 (en) 2002-09-23 2012-04-03 The General Hospital Corporation Three dimensional construct for the design and fabrication of physiological fluidic networks
US8220494B2 (en) 2002-09-25 2012-07-17 California Institute Of Technology Microfluidic large scale integration
US7141386B2 (en) 2002-10-31 2006-11-28 Hewlett-Packard Development Company, L.P. Cell culture device
WO2004046337A2 (en) 2002-11-19 2004-06-03 The Board Of Trustees Of The University Of Illinois Multilayered microcultures
US20050266582A1 (en) 2002-12-16 2005-12-01 Modlin Douglas N Microfluidic system with integrated permeable membrane
WO2004059299A1 (en) 2002-12-16 2004-07-15 Cytodiscovery, Inc. Microfluidic system with integrated permeable membrane
WO2004076608A2 (en) 2003-02-26 2004-09-10 Georgia Tech Research Corporation Bioreactor and methods for tissue growth and conditioning
WO2004101774A1 (ja) 2003-05-14 2004-11-25 Japan Tissue Engineering Co. Ltd 細胞培養方法及び培養組織
KR20060036908A (ko) 2003-05-22 2006-05-02 폴 코포레이션 세포 배양 장치 및 시스템
US6969166B2 (en) 2003-05-29 2005-11-29 3M Innovative Properties Company Method for modifying the surface of a substrate
US20050032208A1 (en) 2003-06-18 2005-02-10 Oh Steve Kah Weng Materials and methods to produce stem cells
GB0321158D0 (en) 2003-09-10 2003-10-08 Central Research Lab Ltd Apparatus and method for handling cells,embryos or oocytes
AU2004280623B2 (en) 2003-10-08 2010-12-02 Wilson Wolf Manufacturing, LLC Cell culture methods and devices utilizing gas permeable materials
EP2267111A3 (en) 2003-11-10 2012-04-25 Wilson Wolf Manufacturing Corporation Compartmentalized device for cell culture, cell processing, and sample dialysis
JP2007515958A (ja) 2003-12-19 2007-06-21 ユニヴァーシティー オブ ウォータールー 培養細胞、細胞培養の方法および機器
US20060112438A1 (en) 2004-01-02 2006-05-25 West Michael D Novel culture systems for ex vivo development
JP2007537759A (ja) 2004-05-19 2007-12-27 マサチューセッツ・インスティテュート・オブ・テクノロジー 灌流三次元細胞／組織疾患モデル
US20080233607A1 (en) 2004-11-11 2008-09-25 Hanry Yu Cell Culture Device
US7022518B1 (en) 2005-01-31 2006-04-04 Glen Feye Apparatus and method for co-culturing of cells
EP1851304A4 (en) 2005-02-23 2009-10-21 Cook William A Australia CULTURE DEVICE
US9354156B2 (en) 2007-02-08 2016-05-31 Emd Millipore Corporation Microfluidic particle analysis method, device and system
US9637715B2 (en) 2005-07-07 2017-05-02 Emd Millipore Corporation Cell culture and invasion assay method and system
ES2865180T3 (es) 2005-07-07 2021-10-15 Univ California Aparato para formación de cultivo celular
US9388374B2 (en) 2005-07-07 2016-07-12 Emd Millipore Corporation Microfluidic cell culture systems
US8257964B2 (en) 2006-01-04 2012-09-04 Cell ASIC Microwell cell-culture device and fabrication method
US7855070B2 (en) 2005-07-08 2010-12-21 Georgia Tech Research Corporation Centimeter-scale, integrated diagnostics incubator for biological culturing
US7195934B2 (en) 2005-07-11 2007-03-27 Applied Materials, Inc. Method and system for deposition tuning in an epitaxial film growth apparatus
US7745209B2 (en) 2005-07-26 2010-06-29 Corning Incorporated Multilayered cell culture apparatus
US20070090166A1 (en) 2005-10-18 2007-04-26 Shuichi Takayama Microfluidic cell culture device
US20070084706A1 (en) 2005-10-18 2007-04-19 Shuichi Takayama Microfluidic cell culture device and method for using same
US20080038713A1 (en) 2005-11-02 2008-02-14 Affymetrix, Inc. System and Method for Biological Assay
WO2007106579A2 (en) 2006-03-15 2007-09-20 Micronics, Inc. Integrated nucleic acid assays
US8293524B2 (en) 2006-03-31 2012-10-23 Fluxion Biosciences Inc. Methods and apparatus for the manipulation of particle suspensions and testing thereof
US9023642B2 (en) 2006-07-07 2015-05-05 The University Of Houston System Method and apparatus for a miniature bioreactor system for long-term cell culture
US20100151571A1 (en) * 2006-07-07 2010-06-17 Georgia Tech Research Corporation Centimeter-scale, integrated diagnostics incubator for biological culturing
CN101611133A (zh) 2006-12-07 2009-12-23 威尔森沃尔夫制造公司 高效装置及培养细胞的方法
EP2175999B1 (en) * 2007-06-21 2017-01-04 Gen-Probe Incorporated Receptacles for use in performing processes
JP2010536565A (ja) * 2007-08-23 2010-12-02 シンベニオ・バイオシステムズ・インコーポレーテッド 目標種用のトラップ用磁気選別システム
WO2009026931A2 (en) * 2007-08-24 2009-03-05 Smart Biosystems Aps Mesoscale bioreactor platform for perfusion
WO2009062095A1 (en) 2007-11-07 2009-05-14 Bellbrook Labs, Llc Microfluidic device having stable static gradient for analyzing chemotaxis
EP2245453B1 (en) 2008-01-03 2016-10-05 EMD Millipore Corporation Microfluidic cell culture array system for automated assays and methods of operation
WO2009102453A2 (en) 2008-02-11 2009-08-20 The General Hospital Corporation System and method for quantitative assessment of biological migration behavior
KR20100136989A (ko) * 2008-03-28 2010-12-29 지멘스 워터 테크놀로지스 코포레이션 호기성 및 혐기성 하이브리드 폐수 및 슬러지 처리 시스템 및 방법
US9157550B2 (en) 2009-01-05 2015-10-13 The Board Of Trustees Of The University Of Illinois Microfluidic systems and methods
WO2010088496A1 (en) 2009-01-30 2010-08-05 Gen-Probe Incorporated Systems and methods for detecting a signal and applying thermal energy to a signal transmission element
CN102803147B (zh) * 2009-06-05 2015-11-25 尹特根埃克斯有限公司 通用样品准备系统以及在一体化分析系统中的用途
US20120164036A1 (en) 2009-07-21 2012-06-28 Seth Stern Microfluidic devices and uses thereof
US9353342B2 (en) 2010-01-21 2016-05-31 Emd Millipore Corporation Cell culture and gradient migration assay methods and devices
CN201803927U (zh) 2010-05-06 2011-04-20 重庆医科大学 用于检测细胞迁移的琼脂糖凝胶微流动腔装置
US20130189671A1 (en) 2010-08-19 2013-07-25 Jan H. Hoh Cell Culture System and Method of Use Thereof
SG10201508047SA (en) 2010-09-29 2015-10-29 Massachusetts Inst Technology Device for high throughput investigations of cellular interactions
US10526572B2 (en) 2011-04-01 2020-01-07 EMD Millipore Corporaticn Cell culture and invasion assay method and system
SG11201402558QA (en) 2011-12-03 2014-06-27 Emd Millipore Corp Micro-incubation systems for microfluidic cell culture and methods

Also Published As

Publication number Publication date
EP2785825B1 (en) 2021-04-21
JP2020188806A (ja) 2020-11-26
ES2870857T3 (es) 2021-10-27
JP2017213001A (ja) 2017-12-07
JP2019162122A (ja) 2019-09-26
US20140287489A1 (en) 2014-09-25
CN111808748A (zh) 2020-10-23
CN107345204A (zh) 2017-11-14
US9206384B2 (en) 2015-12-08
CN111808748B (zh) 2023-11-28
WO2013082612A1 (en) 2013-06-06
US10190085B2 (en) 2019-01-29
JP6526123B2 (ja) 2019-06-05
JP6930050B2 (ja) 2021-09-01
JP7053737B2 (ja) 2022-04-12
EP2785825A4 (en) 2015-08-26
SG10201609393QA (en) 2017-01-27
US20130171679A1 (en) 2013-07-04
SG11201402558QA (en) 2014-06-27
EP2785825A1 (en) 2014-10-08
US9428723B2 (en) 2016-08-30
JP6232383B2 (ja) 2017-11-15
CN104245917A (zh) 2014-12-24
US20160312166A1 (en) 2016-10-27
CN107345204B (zh) 2021-02-12
JP2015500020A (ja) 2015-01-05
CN104245917B (zh) 2017-07-21

Similar Documents

Publication Publication Date Title
SG10201609393QA (en) Micro-incubation systems for microfluidic cell culture and methods
IL288509A (en) Mammalian cell culture
IL254464A0 (en) Methods and systems for seeding cells
SG10201601434RA (en) Cell Culture System
IL232290A (en) Cell Care Systems and Methods
HK1189026A1 (en) Multifunctional bioreactor system and methods for cell sorting and culturing
EP2843036A4 (en) CELL CULTURE SYSTEM AND CELL CULTURE PROCESS
EP2714935A4 (en) SYSTEMS AND METHODS FOR GENETIC AND BIOLOGICAL ANALYSIS
SG11201401913YA (en) Systems and methods for biological analysis
EP2772530A4 (en) CELL CULTURE ASSAY
EP2879794A4 (en) CELL CULTURE
EP2747488A4 (en) CELL CONTROL METHOD AND SYSTEM
EP2725097A4 (en) CELL CULTURE DEVICE AND CELL CULTURE METHOD
GB201214704D0 (en) Stem cell culture method
EP2905330A4 (en) CELL CULTIVATION ELEMENT AND CELL CULTIVATION METHOD
SG11201401911XA (en) Systems and methods for biological analysis
PL2861713T3 (pl) Sposób hodowli komórkowej i system
HUE058968T2 (hu) Sejtkultúra tenyésztésére szolgáló eszköz
PL393251A1 (pl) Mikroukład do hodowli komórkowej