RU2664734C2 - Способ обнаружения внутриклеточных возбудителей инфекции в клетках спермы - Google Patents

Способ обнаружения внутриклеточных возбудителей инфекции в клетках спермы Download PDF

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RU2664734C2
RU2664734C2 RU2014141207A RU2014141207A RU2664734C2 RU 2664734 C2 RU2664734 C2 RU 2664734C2 RU 2014141207 A RU2014141207 A RU 2014141207A RU 2014141207 A RU2014141207 A RU 2014141207A RU 2664734 C2 RU2664734 C2 RU 2664734C2
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virus
sperm
dna
cells
chlamydia
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RU2014141207A
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RU2014141207A (ru
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Вассилиос ЦИЛИВАКОС
Аггелос ГРИЦАПИС
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Вассилиос ЦИЛИВАКОС
Аггелос ГРИЦАПИС
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56905—Protozoa
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56927—Chlamydia
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G01N33/56988—HIV or HTLV
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G01N33/56994—Herpetoviridae, e.g. cytomegalovirus, Epstein-Barr virus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/36—Gynecology or obstetrics
    • G01N2800/367—Infertility, e.g. sperm disorder, ovulatory dysfunction

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  • Tropical Medicine & Parasitology (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • AIDS & HIV (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
RU2014141207A 2012-03-29 2013-03-29 Способ обнаружения внутриклеточных возбудителей инфекции в клетках спермы RU2664734C2 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GR20120100185 2012-03-29
GR20120100185A GR1008033B (el) 2012-03-29 2012-03-29 Μεθοδος διερευνησης της παρουσιας ενδοκυτταριων μολυσματικων παραγοντων σε κυτταρα του σπερματος
PCT/GR2013/000016 WO2013144662A1 (en) 2012-03-29 2013-03-29 Method of intracellular infectious agent detection in sperm cells

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RU2014141207A RU2014141207A (ru) 2016-05-27
RU2664734C2 true RU2664734C2 (ru) 2018-08-22

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US (1) US20150064691A1 (enExample)
EP (1) EP2831588A1 (enExample)
JP (1) JP6301310B2 (enExample)
KR (1) KR20150042146A (enExample)
CN (1) CN104303059B (enExample)
AU (3) AU2013239416A1 (enExample)
CA (1) CA2868707A1 (enExample)
CL (1) CL2014002584A1 (enExample)
GR (1) GR1008033B (enExample)
HK (1) HK1201579A1 (enExample)
IL (1) IL234844A0 (enExample)
IN (1) IN2014DN08201A (enExample)
RU (1) RU2664734C2 (enExample)
SG (2) SG10201704802YA (enExample)
WO (1) WO2013144662A1 (enExample)
ZA (1) ZA201407818B (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2738798C1 (ru) * 2020-08-31 2020-12-16 Василий Васильевич Ашапкин Способ количественного определения вирусного инфицирования сперматозоидов

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111795920B (zh) * 2015-03-23 2024-04-30 赛多利斯生物分析仪器股份有限公司 对生物材料评估腺病毒或腺相关病毒的病毒类型的非缔合的病毒尺寸颗粒
CN107121547A (zh) * 2016-01-21 2017-09-01 广东和信健康科技有限公司 直接免疫荧光检测肠道病毒71型和柯萨奇病毒a16型的试剂盒及其应用
CN111474339A (zh) * 2020-04-17 2020-07-31 浙江康佰裕生物科技有限公司 一种利用荧光标记痘病毒颗粒的方法及其应用
CN115032391A (zh) * 2022-06-21 2022-09-09 成都思瑞多医疗科技有限公司 精子唾液酸酶1/3检测试剂盒及其制备方法、检测精子唾液酸酶1/3表达水平的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003060520A2 (en) * 2001-12-24 2003-07-24 Helen Lee Sample preparation for the detection of infectious agents
US20060099661A1 (en) * 2004-04-16 2006-05-11 Stuart Elizabeth S Detection and quantification of intracellular pathogens

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6387657B1 (en) * 1997-10-29 2002-05-14 Genentech, Inc. WISP polypeptides and nucleic acids encoding same
GB0016920D0 (en) * 2000-07-10 2000-08-30 Univ Cambridge Tech Decondensation of DNA
US20070037742A1 (en) * 2003-07-25 2007-02-15 Laboratories Serono S.A. Use of follicle stimulating hormone for reduction of spermatozoa chromosomal aberration in males

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003060520A2 (en) * 2001-12-24 2003-07-24 Helen Lee Sample preparation for the detection of infectious agents
US20060099661A1 (en) * 2004-04-16 2006-05-11 Stuart Elizabeth S Detection and quantification of intracellular pathogens

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Уреаплазма (Уреаплазмоз). Информация с сайта "ИМЕДИС +", г.Екатеринбург; 2010. стр.1-2 [Найдено 06.04.2017] [он-лайн], Найдено из Интернет: URL: http://imedis-plus.ru/content/%D1%83%D1%80%D0%B5%D0%B0%D0%BF%D0%BB%D0%B0%D0%B7%D0%BC%D0%B0-%D1%83%D1%80%D0%B5%D0%B0%D0%BF%D0%BB%D0%B0%D0%B7%D0%BC%D0%BE%D0%B7. *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2738798C1 (ru) * 2020-08-31 2020-12-16 Василий Васильевич Ашапкин Способ количественного определения вирусного инфицирования сперматозоидов

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HK1201579A1 (en) 2015-09-04
AU2018274968A1 (en) 2019-01-03
IN2014DN08201A (enExample) 2015-05-01
SG10201704802YA (en) 2017-07-28
ZA201407818B (en) 2016-05-25
CN104303059B (zh) 2016-08-17
KR20150042146A (ko) 2015-04-20
SG11201406096QA (en) 2014-10-30
AU2018275005A1 (en) 2019-01-03
JP6301310B2 (ja) 2018-03-28
EP2831588A1 (en) 2015-02-04
CN104303059A (zh) 2015-01-21
CL2014002584A1 (es) 2015-06-19
GR20120100185A (el) 2013-10-15
WO2013144662A1 (en) 2013-10-03
AU2013239416A1 (en) 2014-10-16
JP2015513103A (ja) 2015-04-30
CA2868707A1 (en) 2013-10-03
RU2014141207A (ru) 2016-05-27
GR1008033B (el) 2013-11-18
US20150064691A1 (en) 2015-03-05
IL234844A0 (en) 2014-12-31

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