JP6301310B2 - 精細胞中の細胞内感染因子の検出方法 - Google Patents

精細胞中の細胞内感染因子の検出方法 Download PDF

Info

Publication number
JP6301310B2
JP6301310B2 JP2015502463A JP2015502463A JP6301310B2 JP 6301310 B2 JP6301310 B2 JP 6301310B2 JP 2015502463 A JP2015502463 A JP 2015502463A JP 2015502463 A JP2015502463 A JP 2015502463A JP 6301310 B2 JP6301310 B2 JP 6301310B2
Authority
JP
Japan
Prior art keywords
sperm
dna
virus
cells
chlamydia
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2015502463A
Other languages
English (en)
Japanese (ja)
Other versions
JP2015513103A (ja
Inventor
ツィルヴァコス，ヴァシリオス
グリツァピス，アゲロス
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of JP2015513103A publication Critical patent/JP2015513103A/ja
Application granted granted Critical
Publication of JP6301310B2 publication Critical patent/JP6301310B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56905—Protozoa
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56927—Chlamydia
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G01N33/56988—HIV or HTLV
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G01N33/56994—Herpetoviridae, e.g. cytomegalovirus, Epstein-Barr virus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/36—Gynecology or obstetrics
    • G01N2800/367—Infertility, e.g. sperm disorder, ovulatory dysfunction

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • AIDS & HIV (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2015502463A 2012-03-29 2013-03-29 精細胞中の細胞内感染因子の検出方法 Expired - Fee Related JP6301310B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GR20120100185 2012-03-29
GR20120100185A GR1008033B (el) 2012-03-29 2012-03-29 Μεθοδος διερευνησης της παρουσιας ενδοκυτταριων μολυσματικων παραγοντων σε κυτταρα του σπερματος
PCT/GR2013/000016 WO2013144662A1 (en) 2012-03-29 2013-03-29 Method of intracellular infectious agent detection in sperm cells

Publications (2)

Publication Number Publication Date
JP2015513103A JP2015513103A (ja) 2015-04-30
JP6301310B2 true JP6301310B2 (ja) 2018-03-28

Family

ID=48407746

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2015502463A Expired - Fee Related JP6301310B2 (ja) 2012-03-29 2013-03-29 精細胞中の細胞内感染因子の検出方法

Country Status (16)

Country Link
US (1) US20150064691A1 (enExample)
EP (1) EP2831588A1 (enExample)
JP (1) JP6301310B2 (enExample)
KR (1) KR20150042146A (enExample)
CN (1) CN104303059B (enExample)
AU (3) AU2013239416A1 (enExample)
CA (1) CA2868707A1 (enExample)
CL (1) CL2014002584A1 (enExample)
GR (1) GR1008033B (enExample)
HK (1) HK1201579A1 (enExample)
IL (1) IL234844A0 (enExample)
IN (1) IN2014DN08201A (enExample)
RU (1) RU2664734C2 (enExample)
SG (2) SG10201704802YA (enExample)
WO (1) WO2013144662A1 (enExample)
ZA (1) ZA201407818B (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016154283A1 (en) * 2015-03-23 2016-09-29 Virocyt, Inc. Flow cytometry method for evaluating biological material for unassociated virus-size particles of influenza virus viral type
CN107121547A (zh) * 2016-01-21 2017-09-01 广东和信健康科技有限公司 直接免疫荧光检测肠道病毒71型和柯萨奇病毒a16型的试剂盒及其应用
CN111474339A (zh) * 2020-04-17 2020-07-31 浙江康佰裕生物科技有限公司 一种利用荧光标记痘病毒颗粒的方法及其应用
RU2738798C1 (ru) * 2020-08-31 2020-12-16 Василий Васильевич Ашапкин Способ количественного определения вирусного инфицирования сперматозоидов
CN115032391A (zh) * 2022-06-21 2022-09-09 成都思瑞多医疗科技有限公司 精子唾液酸酶1/3检测试剂盒及其制备方法、检测精子唾液酸酶1/3表达水平的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003533164A (ja) * 1997-10-29 2003-11-11 ジェネンテック・インコーポレーテッド Ｗｎｔ−１誘導分泌ポリペプチド：ＷＩＳＰ−１，−２及び−３
GB0016920D0 (en) * 2000-07-10 2000-08-30 Univ Cambridge Tech Decondensation of DNA
GB0130947D0 (en) * 2001-12-24 2002-02-13 Lee Helen Improved sample preparation for the detection of infectious agents
PL1673105T3 (pl) * 2003-07-25 2007-08-31 Laboratoires Serono S A Zastosowanie hormonu folikulotropowego do zmniejszania aberracji chromosomalnych w gametach u samców
CA2562241A1 (en) * 2004-04-16 2005-12-08 University Of Massachusetts Detection and quantification of intracellular pathogens

Also Published As

Publication number Publication date
GR20120100185A (el) 2013-10-15
GR1008033B (el) 2013-11-18
SG10201704802YA (en) 2017-07-28
WO2013144662A1 (en) 2013-10-03
AU2013239416A1 (en) 2014-10-16
CL2014002584A1 (es) 2015-06-19
ZA201407818B (en) 2016-05-25
AU2018275005A1 (en) 2019-01-03
SG11201406096QA (en) 2014-10-30
RU2664734C2 (ru) 2018-08-22
CN104303059B (zh) 2016-08-17
CA2868707A1 (en) 2013-10-03
EP2831588A1 (en) 2015-02-04
JP2015513103A (ja) 2015-04-30
AU2018274968A1 (en) 2019-01-03
HK1201579A1 (en) 2015-09-04
IN2014DN08201A (enExample) 2015-05-01
US20150064691A1 (en) 2015-03-05
IL234844A0 (en) 2014-12-31
CN104303059A (zh) 2015-01-21
KR20150042146A (ko) 2015-04-20
RU2014141207A (ru) 2016-05-27

Similar Documents

Publication Publication Date Title
Theel et al. Molecular and direct detection tests for Treponema pallidum subspecies pallidum: a review of the literature, 1964–2017
Stamm Diagnosis of Chlamydia trachomatis genitourinary infections
AU760291B2 (en) Flow cytometer for analysis of general diagnostic factors in cells and body fluids
JP6301310B2 (ja) 精細胞中の細胞内感染因子の検出方法
BRPI0714494B1 (pt) ensaio e kit para a previsão de sucesso para implantação em fertilização assistida
RU2362167C1 (ru) Способ диагностики мужского бесплодия
Arunagiri et al. Diagnostic strategies in the era of Monkeypox resurgence: a comprehensive analysis
Gdoura et al. Detection of Chlamydia trachomatis in semen and urethral specimens from male members of infertile couples in Tunisia
Eley How to detect Chlamydia trachomatis in males?
RU2437100C1 (ru) Способ диагностики аномалий упаковки хроматина сперматозоидов при мужском бесплодии
Bjercke et al. Characteristics of women under fertility investigation with IgA/IgG seropositivity for Chlamydia trachomatis
El Sanousi et al. Comparison of real-time PCR versus ELISA in the diagnosis of cytomegalovirus infection in pregnant women
Essa et al. The use of flow cytometry for the detection of CMV‐specific antigen (pp65) in leukocytes of kidney recipients
Muller et al. Association between anti-chlamydial immunity and IVF outcome
Elhassan et al. Bovine herpes virus-1 (BoHV-1) detection in dairy cattle with reproductive problems in Sudan
RU2394496C1 (ru) Способ диагностики сифилиса путем одновременного определения реагиновых и трепонемоспецифических антител к t.pallidum на микроскопных альдегидных слайдах
Mahfouz et al. The diagnostic and therapeutic applications of flow cytometry in male infertility
Suleiman et al. Serological, molecular, and clinical diagnosis of caprine brucellosis in Tikrit, Iraq
Marchesi et al. Current assessment of sperm DNA integrity
RU2582914C2 (ru) Способ диагностики аномалий упаковки хроматина сперматозоидов при мужском бесплодии и диагностикум определения мужского бесплодия
Porge et al. THE EFFECT OF HUMAN PAPILLOMA VIRUS ON SPERMATOGENESIS
CN115290536A (zh) 一种改良的微量淋巴毒交叉配型检测方法
Martins et al. Assessment of Sperm Chromatin Damage by TUNEL Method Using Benchtop Flow Cytometer
Laokirkkiat et al. HBA as a Potential Alternative to the TUNEL Assay for Assessment of DNA Integrity in Asthenozoospermic Spermatozoa
EP1936379A1 (en) Method for the detection of active hpv infection in biological samples by a sandwich enzyme-linked immunosorbent assay and test kit for performing the method

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20160324

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20170222

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170228

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170531

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170731

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170831

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20180130

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20180228

R150 Certificate of patent or registration of utility model

Ref document number: 6301310

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

LAPS Cancellation because of no payment of annual fees