CA2868707A1 - Method of intracellular infectious agent detection in sperm cells - Google Patents

Method of intracellular infectious agent detection in sperm cells Download PDF

Info

Publication number
CA2868707A1
CA2868707A1 CA2868707A CA2868707A CA2868707A1 CA 2868707 A1 CA2868707 A1 CA 2868707A1 CA 2868707 A CA2868707 A CA 2868707A CA 2868707 A CA2868707 A CA 2868707A CA 2868707 A1 CA2868707 A1 CA 2868707A1
Authority
CA
Canada
Prior art keywords
dna
spermatozoa
virus
cells
chlamydia
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2868707A
Other languages
English (en)
French (fr)
Inventor
Vassilios Tsilivakos
Aggelos GRITZAPIS
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2868707A1 publication Critical patent/CA2868707A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56905—Protozoa
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56927—Chlamydia
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G01N33/56988—HIV or HTLV
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G01N33/56994—Herpetoviridae, e.g. cytomegalovirus, Epstein-Barr virus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/36—Gynecology or obstetrics
    • G01N2800/367—Infertility, e.g. sperm disorder, ovulatory dysfunction

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • AIDS & HIV (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2868707A 2012-03-29 2013-03-29 Method of intracellular infectious agent detection in sperm cells Abandoned CA2868707A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GR20120100185 2012-03-29
GR20120100185A GR1008033B (el) 2012-03-29 2012-03-29 Μεθοδος διερευνησης της παρουσιας ενδοκυτταριων μολυσματικων παραγοντων σε κυτταρα του σπερματος
PCT/GR2013/000016 WO2013144662A1 (en) 2012-03-29 2013-03-29 Method of intracellular infectious agent detection in sperm cells

Publications (1)

Publication Number Publication Date
CA2868707A1 true CA2868707A1 (en) 2013-10-03

Family

ID=48407746

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2868707A Abandoned CA2868707A1 (en) 2012-03-29 2013-03-29 Method of intracellular infectious agent detection in sperm cells

Country Status (16)

Country Link
US (1) US20150064691A1 (enExample)
EP (1) EP2831588A1 (enExample)
JP (1) JP6301310B2 (enExample)
KR (1) KR20150042146A (enExample)
CN (1) CN104303059B (enExample)
AU (3) AU2013239416A1 (enExample)
CA (1) CA2868707A1 (enExample)
CL (1) CL2014002584A1 (enExample)
GR (1) GR1008033B (enExample)
HK (1) HK1201579A1 (enExample)
IL (1) IL234844A0 (enExample)
IN (1) IN2014DN08201A (enExample)
RU (1) RU2664734C2 (enExample)
SG (2) SG10201704802YA (enExample)
WO (1) WO2013144662A1 (enExample)
ZA (1) ZA201407818B (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111795920B (zh) * 2015-03-23 2024-04-30 赛多利斯生物分析仪器股份有限公司 对生物材料评估腺病毒或腺相关病毒的病毒类型的非缔合的病毒尺寸颗粒
CN107121547A (zh) * 2016-01-21 2017-09-01 广东和信健康科技有限公司 直接免疫荧光检测肠道病毒71型和柯萨奇病毒a16型的试剂盒及其应用
CN111474339A (zh) * 2020-04-17 2020-07-31 浙江康佰裕生物科技有限公司 一种利用荧光标记痘病毒颗粒的方法及其应用
RU2738798C1 (ru) * 2020-08-31 2020-12-16 Василий Васильевич Ашапкин Способ количественного определения вирусного инфицирования сперматозоидов
CN115032391A (zh) * 2022-06-21 2022-09-09 成都思瑞多医疗科技有限公司 精子唾液酸酶1/3检测试剂盒及其制备方法、检测精子唾液酸酶1/3表达水平的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6387657B1 (en) * 1997-10-29 2002-05-14 Genentech, Inc. WISP polypeptides and nucleic acids encoding same
GB0016920D0 (en) * 2000-07-10 2000-08-30 Univ Cambridge Tech Decondensation of DNA
GB0130947D0 (en) * 2001-12-24 2002-02-13 Lee Helen Improved sample preparation for the detection of infectious agents
US20070037742A1 (en) * 2003-07-25 2007-02-15 Laboratories Serono S.A. Use of follicle stimulating hormone for reduction of spermatozoa chromosomal aberration in males
CA2562241A1 (en) * 2004-04-16 2005-12-08 University Of Massachusetts Detection and quantification of intracellular pathogens

Also Published As

Publication number Publication date
HK1201579A1 (en) 2015-09-04
RU2664734C2 (ru) 2018-08-22
AU2018274968A1 (en) 2019-01-03
IN2014DN08201A (enExample) 2015-05-01
SG10201704802YA (en) 2017-07-28
ZA201407818B (en) 2016-05-25
CN104303059B (zh) 2016-08-17
KR20150042146A (ko) 2015-04-20
SG11201406096QA (en) 2014-10-30
AU2018275005A1 (en) 2019-01-03
JP6301310B2 (ja) 2018-03-28
EP2831588A1 (en) 2015-02-04
CN104303059A (zh) 2015-01-21
CL2014002584A1 (es) 2015-06-19
GR20120100185A (el) 2013-10-15
WO2013144662A1 (en) 2013-10-03
AU2013239416A1 (en) 2014-10-16
JP2015513103A (ja) 2015-04-30
RU2014141207A (ru) 2016-05-27
GR1008033B (el) 2013-11-18
US20150064691A1 (en) 2015-03-05
IL234844A0 (en) 2014-12-31

Similar Documents

Publication Publication Date Title
AU2018274968A1 (en) Method of intracellular infectious agent detection in sperm cells
Zhang et al. Early apoptotic changes in human spermatozoa and their relationships with conventional semen parameters and sperm DNA fragmentation
Garrett et al. Clinical application of sperm-oocyte interaction tests in in vitro fertilization–embryo transfer and intracytoplasmic sperm injection programs
Barbăroșie et al. Diagnostic value of advanced semen analysis in evaluation of male infertility
Sigman et al. Semen analysis and sperm function assays: what do they mean?
Sellami et al. Assessment of chromatin maturity in human spermatozoa: useful aniline blue assay for routine diagnosis of male infertility
Ajina et al. Assessment of human sperm DNA integrity using two cytochemical tests: Acridine orange test and toluidine blue assay
Varum et al. Characterization of human sperm populations using conventional parameters, surface ubiquitination, and apoptotic markers
Reyes-Méndez et al. Comparison of two diagnostic algorithms for the identification of patients with HCV viremia using a new HCV antigen test
US5935800A (en) Assays and kits for determining male fertility
Yániz et al. Use of image analysis to assess the plasma membrane integrity of ram spermatozoa in different diluents
Dolník et al. Flow cytometry in assessment of sperm integrity and functionality–a review
Faduola et al. Sperm chromatin structure assay results in Nigerian men with unexplained infertility
Habermann et al. Altered sperm function or sperm antibodies are not associated with chlamydial antibodies in infertile men with leucocytospermia
Makarounis et al. Detection of Chlamydia trachomatis inside spermatozoa using flow cytometry: Effects of antibiotic treatment (before and after) on sperm count parameters
Cheuquemán et al. Sperm membrane functionality in the dog assessed by flow cytometry
Hodjat et al. Increased sperm ubiquitination correlates with abnormal chromatin integrity
Fathi et al. Flow cytometry: a novel approach for indirect assessment of protamine deficiency by CMA3 staining, taking into account the presence of M540 or apoptotic bodies
Sellami et al. A proposed mouse model to study male infertility provoked by genital serovar E, Chlamydia trachomatis
Martins et al. Assessment of Sperm Chromatin Damage by TUNEL Method Using Benchtop Flow Cytometer
Zoca et al. Use of Sperm In Vitro Capacitation and Flow Cytometry to Estimate Bull Fertility
Laokirkkiat et al. HBA as a Potential Alternative to the TUNEL Assay for Assessment of DNA Integrity in Asthenozoospermic Spermatozoa
Vidya et al. Semen analysis revisited-Qualitative assessment of sperms using cytochemical stains-the new norms of male infertility workup
CN115290536A (zh) 一种改良的微量淋巴毒交叉配型检测方法
CA3006360A1 (en) Non-invasive method to assess embryo competence

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20180313

FZDE Discontinued

Effective date: 20200831