RU2662172C2 - Способ получения регенеративного ветеринарного препарата на основе экстракта мезенхимальных стволовых клеток и кондиционной среды - Google Patents
Способ получения регенеративного ветеринарного препарата на основе экстракта мезенхимальных стволовых клеток и кондиционной среды Download PDFInfo
- Publication number
- RU2662172C2 RU2662172C2 RU2016148715A RU2016148715A RU2662172C2 RU 2662172 C2 RU2662172 C2 RU 2662172C2 RU 2016148715 A RU2016148715 A RU 2016148715A RU 2016148715 A RU2016148715 A RU 2016148715A RU 2662172 C2 RU2662172 C2 RU 2662172C2
- Authority
- RU
- Russia
- Prior art keywords
- regenerative
- cells
- medium
- stem cells
- conditioned medium
- Prior art date
Links
- 239000003636 conditioned culture medium Substances 0.000 title claims abstract description 33
- 239000000284 extract Substances 0.000 title claims abstract description 25
- 230000001172 regenerating effect Effects 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 238000004519 manufacturing process Methods 0.000 title abstract description 7
- 210000000130 stem cell Anatomy 0.000 title description 6
- 210000002901 mesenchymal stem cell Anatomy 0.000 claims abstract description 48
- 239000002609 medium Substances 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 22
- 210000001185 bone marrow Anatomy 0.000 claims abstract description 19
- 210000000577 adipose tissue Anatomy 0.000 claims abstract description 14
- 241001465754 Metazoa Species 0.000 claims abstract description 13
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000006285 cell suspension Substances 0.000 claims abstract description 9
- 238000004108 freeze drying Methods 0.000 claims abstract description 8
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims abstract description 6
- 239000004471 Glycine Substances 0.000 claims abstract description 6
- 108090000174 Interleukin-10 Proteins 0.000 claims abstract description 6
- 108090001005 Interleukin-6 Proteins 0.000 claims abstract description 6
- 108010063738 Interleukins Proteins 0.000 claims abstract description 6
- 102000015696 Interleukins Human genes 0.000 claims abstract description 6
- 229940047122 interleukins Drugs 0.000 claims abstract description 6
- 238000012258 culturing Methods 0.000 claims abstract description 5
- 239000012091 fetal bovine serum Substances 0.000 claims abstract description 5
- 238000010257 thawing Methods 0.000 claims abstract description 5
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 235000015097 nutrients Nutrition 0.000 claims abstract description 4
- 230000001954 sterilising effect Effects 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims abstract 2
- 238000007710 freezing Methods 0.000 claims description 2
- 230000008014 freezing Effects 0.000 claims description 2
- 239000003814 drug Substances 0.000 abstract description 28
- 230000000694 effects Effects 0.000 abstract description 15
- 108010002350 Interleukin-2 Proteins 0.000 abstract description 5
- 230000000087 stabilizing effect Effects 0.000 abstract description 2
- 239000000273 veterinary drug Substances 0.000 abstract 3
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 46
- 210000004271 bone marrow stromal cell Anatomy 0.000 description 27
- 229940079593 drug Drugs 0.000 description 21
- 241000700159 Rattus Species 0.000 description 16
- 239000001963 growth medium Substances 0.000 description 15
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 11
- 230000012010 growth Effects 0.000 description 11
- 238000004113 cell culture Methods 0.000 description 9
- 230000001413 cellular effect Effects 0.000 description 9
- 230000001143 conditioned effect Effects 0.000 description 9
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 7
- 239000002953 phosphate buffered saline Substances 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 210000004185 liver Anatomy 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 230000001225 therapeutic effect Effects 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 5
- 229930182566 Gentamicin Natural products 0.000 description 5
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 206010067125 Liver injury Diseases 0.000 description 5
- 230000004069 differentiation Effects 0.000 description 5
- 229960002518 gentamicin Drugs 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- 230000007170 pathology Effects 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 230000002062 proliferating effect Effects 0.000 description 5
- 230000035755 proliferation Effects 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- 102100036475 Alanine aminotransferase 1 Human genes 0.000 description 4
- 108010082126 Alanine transaminase Proteins 0.000 description 4
- 102000004127 Cytokines Human genes 0.000 description 4
- 108090000695 Cytokines Proteins 0.000 description 4
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 4
- 229930182816 L-glutamine Natural products 0.000 description 4
- 239000013543 active substance Substances 0.000 description 4
- 230000001154 acute effect Effects 0.000 description 4
- APKFDSVGJQXUKY-INPOYWNPSA-N amphotericin B Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C=C/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 APKFDSVGJQXUKY-INPOYWNPSA-N 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000002054 inoculum Substances 0.000 description 4
- 238000002955 isolation Methods 0.000 description 4
- 239000008194 pharmaceutical composition Substances 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- APKFDSVGJQXUKY-KKGHZKTASA-N Amphotericin-B Natural products O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1C=CC=CC=CC=CC=CC=CC=C[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 APKFDSVGJQXUKY-KKGHZKTASA-N 0.000 description 3
- 102000004142 Trypsin Human genes 0.000 description 3
- 108090000631 Trypsin Proteins 0.000 description 3
- 206010052428 Wound Diseases 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 3
- 229960003942 amphotericin b Drugs 0.000 description 3
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 239000003937 drug carrier Substances 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 231100000234 hepatic damage Toxicity 0.000 description 3
- 230000008818 liver damage Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000000386 microscopy Methods 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 230000008929 regeneration Effects 0.000 description 3
- 238000011069 regeneration method Methods 0.000 description 3
- 230000004936 stimulating effect Effects 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- NMUSYJAQQFHJEW-UHFFFAOYSA-N 5-Azacytidine Natural products O=C1N=C(N)N=CN1C1C(O)C(O)C(CO)O1 NMUSYJAQQFHJEW-UHFFFAOYSA-N 0.000 description 2
- NMUSYJAQQFHJEW-KVTDHHQDSA-N 5-azacytidine Chemical compound O=C1N=C(N)N=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](CO)O1 NMUSYJAQQFHJEW-KVTDHHQDSA-N 0.000 description 2
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000001857 anti-mycotic effect Effects 0.000 description 2
- 239000002543 antimycotic Substances 0.000 description 2
- 229960002756 azacitidine Drugs 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 210000005013 brain tissue Anatomy 0.000 description 2
- 210000004413 cardiac myocyte Anatomy 0.000 description 2
- 230000022131 cell cycle Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 210000001808 exosome Anatomy 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 239000003102 growth factor Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 230000000144 pharmacologic effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000002356 single layer Substances 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000012588 trypsin Substances 0.000 description 2
- 230000035899 viability Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- UZOVYGYOLBIAJR-UHFFFAOYSA-N 4-isocyanato-4'-methyldiphenylmethane Chemical compound C1=CC(C)=CC=C1CC1=CC=C(N=C=O)C=C1 UZOVYGYOLBIAJR-UHFFFAOYSA-N 0.000 description 1
- 229930183010 Amphotericin Natural products 0.000 description 1
- QGGFZZLFKABGNL-UHFFFAOYSA-N Amphotericin A Natural products OC1C(N)C(O)C(C)OC1OC1C=CC=CC=CC=CCCC=CC=CC(C)C(O)C(C)C(C)OC(=O)CC(O)CC(O)CCC(O)C(O)CC(O)CC(O)(CC(O)C2C(O)=O)OC2C1 QGGFZZLFKABGNL-UHFFFAOYSA-N 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 description 1
- 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 108010002352 Interleukin-1 Proteins 0.000 description 1
- 206010028400 Mutagenic effect Diseases 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 206010053615 Thermal burn Diseases 0.000 description 1
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229940009444 amphotericin Drugs 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 210000001557 animal structure Anatomy 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- OHJMTUPIZMNBFR-UHFFFAOYSA-N biuret Chemical compound NC(=O)NC(N)=O OHJMTUPIZMNBFR-UHFFFAOYSA-N 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000013553 cell monolayer Substances 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- RNFNDJAIBTYOQL-UHFFFAOYSA-N chloral hydrate Chemical compound OC(O)C(Cl)(Cl)Cl RNFNDJAIBTYOQL-UHFFFAOYSA-N 0.000 description 1
- 229960002327 chloral hydrate Drugs 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 239000002577 cryoprotective agent Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 239000012649 demethylating agent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 238000002296 dynamic light scattering Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 239000006481 glucose medium Substances 0.000 description 1
- 229960002743 glutamine Drugs 0.000 description 1
- 244000144993 groups of animals Species 0.000 description 1
- 230000003779 hair growth Effects 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000004217 heart function Effects 0.000 description 1
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 1
- 210000003494 hepatocyte Anatomy 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
- 238000007912 intraperitoneal administration Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 210000002346 musculoskeletal system Anatomy 0.000 description 1
- 231100000243 mutagenic effect Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 208000010125 myocardial infarction Diseases 0.000 description 1
- 208000015122 neurodegenerative disease Diseases 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000004768 organ dysfunction Effects 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 210000000963 osteoblast Anatomy 0.000 description 1
- 206010033675 panniculitis Diseases 0.000 description 1
- 229960005489 paracetamol Drugs 0.000 description 1
- 230000003076 paracrine Effects 0.000 description 1
- 230000007310 pathophysiology Effects 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 210000000512 proximal kidney tubule Anatomy 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000002784 sclerotic effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000009759 skin aging Effects 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000000021 stimulant Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 210000004003 subcutaneous fat Anatomy 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000036575 thermal burns Effects 0.000 description 1
- 210000001694 thigh bone Anatomy 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000002110 toxicologic effect Effects 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 238000000825 ultraviolet detection Methods 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2016148715A RU2662172C2 (ru) | 2016-12-13 | 2016-12-13 | Способ получения регенеративного ветеринарного препарата на основе экстракта мезенхимальных стволовых клеток и кондиционной среды |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2016148715A RU2662172C2 (ru) | 2016-12-13 | 2016-12-13 | Способ получения регенеративного ветеринарного препарата на основе экстракта мезенхимальных стволовых клеток и кондиционной среды |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| RU2016148715A RU2016148715A (ru) | 2018-06-13 |
| RU2016148715A3 RU2016148715A3 (fr) | 2018-06-13 |
| RU2662172C2 true RU2662172C2 (ru) | 2018-07-24 |
Family
ID=62619402
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2016148715A RU2662172C2 (ru) | 2016-12-13 | 2016-12-13 | Способ получения регенеративного ветеринарного препарата на основе экстракта мезенхимальных стволовых клеток и кондиционной среды |
Country Status (1)
| Country | Link |
|---|---|
| RU (1) | RU2662172C2 (fr) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2720800C1 (ru) * | 2018-11-28 | 2020-05-13 | Общество с ограниченной ответственностью "НОВИСТЕМ" | Средство для крупного рогатого скота, обладающее противовоспалительной, регенеративной, иммуномодулирующей, детоксикационной, адаптогенной активностью и способное регулировать метаболизм |
| RU2720812C1 (ru) * | 2018-11-28 | 2020-05-13 | Общество с ограниченной ответственностью "НОВИСТЕМ" | Способ получения средства, обладающего противовоспалительной, регенеративной, иммуномодулирующей, детоксикационной, адаптогенной активностью и способного регулировать метаболизм |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2111426C1 (ru) * | 1995-11-03 | 1998-05-20 | Государственный научный центр вирусологии и биотехнологии "Вектор" | Способ лиофильной сушки биопрепарата |
| RU2178309C2 (ru) * | 2000-01-12 | 2002-01-20 | Российский научно-исследовательский институт геронтологии | Антитимоцитарный глобулин для внутривенного введения и способ его получения |
| RU2341270C2 (ru) * | 2006-12-28 | 2008-12-20 | Александр Сергеевич Ботин | Композиция для стимулирования роста и регенерации клеток, а также способы ее получения |
| RU2409350C2 (ru) * | 2005-03-08 | 2011-01-20 | Интервет Интернэшнл Б.В. | Химически определенный стабилизатор |
| RU2495565C1 (ru) * | 2012-10-03 | 2013-10-20 | Игорь Николаевич Габриэль | Способ стимуляции роста и повышения резистентности сельскохозяйственных животных |
-
2016
- 2016-12-13 RU RU2016148715A patent/RU2662172C2/ru not_active IP Right Cessation
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2111426C1 (ru) * | 1995-11-03 | 1998-05-20 | Государственный научный центр вирусологии и биотехнологии "Вектор" | Способ лиофильной сушки биопрепарата |
| RU2178309C2 (ru) * | 2000-01-12 | 2002-01-20 | Российский научно-исследовательский институт геронтологии | Антитимоцитарный глобулин для внутривенного введения и способ его получения |
| RU2409350C2 (ru) * | 2005-03-08 | 2011-01-20 | Интервет Интернэшнл Б.В. | Химически определенный стабилизатор |
| RU2341270C2 (ru) * | 2006-12-28 | 2008-12-20 | Александр Сергеевич Ботин | Композиция для стимулирования роста и регенерации клеток, а также способы ее получения |
| RU2495565C1 (ru) * | 2012-10-03 | 2013-10-20 | Игорь Николаевич Габриэль | Способ стимуляции роста и повышения резистентности сельскохозяйственных животных |
Non-Patent Citations (3)
| Title |
|---|
| АНДРЕЕВА Е.Р. "Мультипотентные мезенхимальные стромальные клетки при моделировании тканевой ("физиологической") гипоксии in vitro" // Авто дмн, Москва, 2016, стр.17, 18, 24, [он-лайн], [найдено 03.11.2017]. * |
| АНДРЕЕВА Е.Р. "Мультипотентные мезенхимальные стромальные клетки при моделировании тканевой ("физиологической") гипоксии in vitro" // Автореферат дмн, Москва, 2016, стр.17, 18, 24, [он-лайн], [найдено 03.11.2017]. Найдено из Интернет: URL: http://www.imbp.ru/webpages/win1251/Science/DisserSov/Andreeva2016/Andreeva-ref.pdf. * |
| Найдено из Интернет: URL: http://www.imbp.ru/webpages/win1251/Science/DisserSov/Andreeva2016/Andreeva-ref.pdf. * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2720800C1 (ru) * | 2018-11-28 | 2020-05-13 | Общество с ограниченной ответственностью "НОВИСТЕМ" | Средство для крупного рогатого скота, обладающее противовоспалительной, регенеративной, иммуномодулирующей, детоксикационной, адаптогенной активностью и способное регулировать метаболизм |
| RU2720812C1 (ru) * | 2018-11-28 | 2020-05-13 | Общество с ограниченной ответственностью "НОВИСТЕМ" | Способ получения средства, обладающего противовоспалительной, регенеративной, иммуномодулирующей, детоксикационной, адаптогенной активностью и способного регулировать метаболизм |
Also Published As
| Publication number | Publication date |
|---|---|
| RU2016148715A (ru) | 2018-06-13 |
| RU2016148715A3 (fr) | 2018-06-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Yang et al. | Comparison of mesenchymal stem cells derived from gingival tissue and periodontal ligament in different incubation conditions | |
| CN106109496B (zh) | 人脐带间充质干细胞提取物冻干粉及制备方法 | |
| CN103070161B (zh) | 一种脂肪间充质干细胞冻存液及冻存方法 | |
| CN105112362B (zh) | 一种胎盘间充质干细胞的无血清培养基及其制备方法 | |
| RU2323252C1 (ru) | Способ культивирования мезенхимальных стволовых клеток человека ex vivo | |
| JP2010538681A (ja) | 人または動物胚から間葉系幹細胞を抽出及びその分泌物を抽出する方法 | |
| Veron et al. | Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera | |
| US20200360443A1 (en) | Stem cell material and method of manufacturing | |
| US20170240856A1 (en) | Placenta-derived potential cells and preparing method thereof | |
| CN110257328A (zh) | 一种间充质干细胞无血清培养基 | |
| Liang et al. | Progress in the treatment of osteoarthritis with umbilical cord stem cells | |
| Schwarz et al. | Characterization of adipose-derived equine and canine mesenchymal stem cells after incubation in agarose-hydrogel | |
| JP2017104091A (ja) | 間葉系細胞の製造方法 | |
| RU2662172C2 (ru) | Способ получения регенеративного ветеринарного препарата на основе экстракта мезенхимальных стволовых клеток и кондиционной среды | |
| CN106701670A (zh) | 一种增强间充质干细胞分泌生物活性因子能力及培养液中活性因子的提取方法 | |
| RU2640556C2 (ru) | Культуральная среда для мезенхимальных стволовых клеток человека | |
| JP2022501034A (ja) | 軟骨組織の生成に使用される単離された中隔軟骨エクソソーム | |
| Samadikuchaksaraei et al. | How does the supernatant of Lactobacillus acidophilus affect the proliferation and differentiation activities of rat bone marrow-derived stromal cells? | |
| Chailakhyan et al. | Proline-rich hypothalamic polypeptide has opposite effects on the proliferation of human normal bone marrow stromal cells and human giant-cell tumour stromal cells | |
| Putra et al. | Evaluation of secretome tenogenic potential from adipose stem cells (ACS) in hypoxic condition with fresh frozen tendon scaffold using scleraxis (Scx), insulin-like growth factor 1 (IGF-1) and collagen type 1 | |
| RU2812015C1 (ru) | Способ получения и применения сфероидов мезенхимальных стволовых клеток для лечения травм кожи | |
| KR101538969B1 (ko) | 감태 추출물을 포함하는 중간엽 줄기세포 배양용 배지 조성물 | |
| Mobasheri et al. | Applications of mesenchymal stem cells in cartilage tissue engineering-part 1 | |
| JP2007525193A (ja) | 臨床用及び商用幹細胞 | |
| KR20220149252A (ko) | 쿠민 추출물을 유효성분으로 포함하는 줄기세포의 연골세포로의 분화 유도용 조성물 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20191214 |
|
| TK4A | Correction to the publication in the bulletin (patent) |
Free format text: CORRECTION TO CHAPTER -MM4A- IN JOURNAL 28-2020 |