RU2652308C2 - Способы и продукты для заживления ран тканей - Google Patents

Способы и продукты для заживления ран тканей Download PDF

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Publication number
RU2652308C2
RU2652308C2 RU2014112784A RU2014112784A RU2652308C2 RU 2652308 C2 RU2652308 C2 RU 2652308C2 RU 2014112784 A RU2014112784 A RU 2014112784A RU 2014112784 A RU2014112784 A RU 2014112784A RU 2652308 C2 RU2652308 C2 RU 2652308C2
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Russia
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unsubstituted
alkyl
substituted
group
wound
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RU2014112784A
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English (en)
Russian (ru)
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RU2014112784A (ru
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Карл ГЕНБЕРГ
Пол Б. САВАЖ
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Брайем Янг Юниверсити
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/56Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
    • A61K31/575Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids substituted in position 17 beta by a chain of three or more carbon atoms, e.g. cholane, cholestane, ergosterol, sitosterol
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00Drugs for dermatological disorders
    • A61P17/02Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00Drugs for skeletal disorders
    • A61P19/08Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00Drugs for skeletal disorders
    • A61P19/08Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A61P19/10Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04Antibacterial agents

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  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Epidemiology (AREA)
  • Rheumatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Engineering & Computer Science (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Dermatology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Steroid Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Materials For Medical Uses (AREA)
RU2014112784A 2011-09-13 2012-09-13 Способы и продукты для заживления ран тканей RU2652308C2 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161534194P 2011-09-13 2011-09-13
US61/534,194 2011-09-13
PCT/US2012/055248 WO2013040269A1 (en) 2011-09-13 2012-09-13 Products for healing of tissue wounds

Publications (2)

Publication Number Publication Date
RU2014112784A RU2014112784A (ru) 2015-10-20
RU2652308C2 true RU2652308C2 (ru) 2018-04-25

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU2014112784A RU2652308C2 (ru) 2011-09-13 2012-09-13 Способы и продукты для заживления ран тканей

Country Status (12)

Country Link
US (1) US9161942B2 (OSRAM)
EP (1) EP2760454B1 (OSRAM)
JP (1) JP6038155B2 (OSRAM)
KR (1) KR101966293B1 (OSRAM)
CN (1) CN104080458B (OSRAM)
AU (2) AU2012308526B2 (OSRAM)
BR (1) BR112014005914B1 (OSRAM)
CA (1) CA2848567C (OSRAM)
IL (1) IL231509B (OSRAM)
MX (1) MX369385B (OSRAM)
RU (1) RU2652308C2 (OSRAM)
WO (2) WO2013040269A1 (OSRAM)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2303026B1 (en) 2008-06-17 2020-09-09 Brigham Young University Cationic steroid antimicrobial diagnostic, detection, screening and imaging methods
MX340644B (es) 2011-07-20 2016-07-19 Univ Brigham Young Compuestos de ceragenina hidrófobos y dispositivos que incorporan los mismos.
US8945217B2 (en) 2011-08-25 2015-02-03 Brigham Young University Medical devices incorporating ceragenin-containing composites
US9694019B2 (en) 2011-09-13 2017-07-04 Brigham Young University Compositions and methods for treating bone diseases and broken bones
CA2852989C (en) 2011-09-13 2021-06-29 Brigham Young University Compositions for treating bone diseases and broken bones
US9603859B2 (en) 2011-09-13 2017-03-28 Brigham Young University Methods and products for increasing the rate of healing of tissue wounds
AU2011385377B2 (en) 2011-12-21 2017-06-01 Brigham Young University Oral care compositions
US9533063B1 (en) 2012-03-01 2017-01-03 Brigham Young University Aerosols incorporating ceragenin compounds and methods of use thereof
EP2846634A2 (en) 2012-05-02 2015-03-18 Brigham Young University Ceragenin particulate materials and methods for making same
NZ707003A (en) 2012-10-17 2016-04-29 Univ Brigham Young Treatment and prevention of mastitis
KR102203375B1 (ko) 2013-01-07 2021-01-15 브라이엄 영 유니버시티 세포 증식을 감소시키는 방법 및 소정의 질환을 치료하는 방법
CN105209465A (zh) 2013-03-15 2015-12-30 布莱阿姆青年大学 治疗炎症、自身免疫性疾病和疼痛的方法
US11524015B2 (en) 2013-03-15 2022-12-13 Brigham Young University Methods for treating inflammation, autoimmune disorders and pain
US10568893B2 (en) 2013-03-15 2020-02-25 Brigham Young University Methods for treating inflammation, autoimmune disorders and pain
US9387215B2 (en) * 2013-04-22 2016-07-12 Brigham Young University Animal feed including cationic cholesterol additive and related methods
US11690855B2 (en) 2013-10-17 2023-07-04 Brigham Young University Methods for treating lung infections and inflammation
US20150203527A1 (en) * 2014-01-23 2015-07-23 Brigham Young University Cationic steroidal antimicrobials
CA2844321C (en) 2014-02-27 2021-03-16 Brigham Young University Cationic steroidal antimicrobial compounds
US9867836B2 (en) 2014-03-13 2018-01-16 Brigham Young University Lavage and/or infusion using CSA compounds for increasing fertility in a mammal
US10220045B2 (en) 2014-03-13 2019-03-05 Brigham Young University Compositions and methods for forming stabilized compositions with reduced CSA agglomeration
US9931350B2 (en) * 2014-03-14 2018-04-03 Brigham Young University Anti-infective and osteogenic compositions and methods of use
US9686966B2 (en) 2014-04-30 2017-06-27 Brigham Young University Methods and apparatus for cleaning or disinfecting a water delivery system
US10441595B2 (en) * 2014-06-26 2019-10-15 Brigham Young University Methods for treating fungal infections
US10238665B2 (en) 2014-06-26 2019-03-26 Brigham Young University Methods for treating fungal infections
US10227376B2 (en) 2014-08-22 2019-03-12 Brigham Young University Radiolabeled cationic steroid antimicrobials and diagnostic methods
US10155788B2 (en) 2014-10-07 2018-12-18 Brigham Young University Cationic steroidal antimicrobial prodrug compositions and uses thereof
US9527883B2 (en) 2015-04-22 2016-12-27 Brigham Young University Methods for the synthesis of ceragenins
WO2016172543A1 (en) 2015-04-22 2016-10-27 Savage Paul B Methods for the synthesis of ceragenins
CN108348484B (zh) * 2015-04-22 2021-12-17 布莱阿姆青年大学 阳离子甾体抗微生物盐
WO2016172534A1 (en) * 2015-04-22 2016-10-27 Savage Paul B Cationic steroidal antimicrobial salts
US9434759B1 (en) * 2015-05-18 2016-09-06 Brigham Young University Cationic steroidal antimicrobial compounds and methods of manufacturing such compounds
US20170080128A1 (en) * 2015-09-21 2017-03-23 Brigham Young University Novel endotracheal tube for the reduction of intubation-related complication in neonates and babies
US10226550B2 (en) 2016-03-11 2019-03-12 Brigham Young University Cationic steroidal antimicrobial compositions for the treatment of dermal tissue
US10959433B2 (en) 2017-03-21 2021-03-30 Brigham Young University Use of cationic steroidal antimicrobials for sporicidal activity
KR101792535B1 (ko) 2017-09-27 2017-11-02 오상자이엘 주식회사 화상 치료용 키트
US12186328B2 (en) 2019-05-23 2025-01-07 Brigham Young University Use of CSA compounds to stimulate stem cells and hair growth

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US428800A (en) * 1890-05-27 miller
US6767904B2 (en) * 1998-03-06 2004-07-27 Bringham Young University Steroid derived antibiotics

Family Cites Families (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4661341A (en) 1984-10-30 1987-04-28 The Procter & Gamble Company Oral compositions
US4842593A (en) 1987-10-09 1989-06-27 The Procter & Gamble Company Disposable absorbent articles for incontinent individuals
US4865855A (en) 1988-01-11 1989-09-12 Kimberly-Clark Corporation Antimicrobial absorbent food pad
US4990338A (en) 1988-05-09 1991-02-05 Dow Corning Corporation Antimicrobial superabsorbent compositions and methods
US5356630A (en) 1989-02-22 1994-10-18 Massachusetts Institute Of Technology Delivery system for controlled release of bioactive factors
US4972848A (en) 1989-08-23 1990-11-27 Medtronic, Inc. Medical electrical lead with polymeric monolithic controlled release device and method of manufacture
GB9020075D0 (en) 1990-09-14 1990-10-24 Filler Aaron G Contrast agents for magnetic resonance imaging of axonal transport
US5286479A (en) 1991-12-10 1994-02-15 The Dow Chemical Company Oral compositions for suppressing mouth odors
ES2157254T3 (es) 1993-03-12 2001-08-16 Upjohn Co Ceftiofur (acido libre) cristalino.
JPH09509959A (ja) 1994-03-10 1997-10-07 マガイニン ファーマシューティカルズ インク. ステロイド誘導体、それらを含む薬剤組成物、及びそれらの薬剤学的用途
US6132765A (en) 1996-04-12 2000-10-17 Uroteq Inc. Drug delivery via therapeutic hydrogels
CA2632790A1 (en) * 1996-07-22 1998-01-29 Renovo Limited Use of sex steroid function modulators to treat wounds and fibrotic disorders
US6350738B1 (en) 1998-03-06 2002-02-26 Brigham Young University Steroid derived antibiotics
ATE269086T1 (de) 1998-03-06 2004-07-15 Univ Brigham Young Antibiotische steroidderivate
US6667299B1 (en) 2000-03-16 2003-12-23 Hollis-Eden Pharmaceuticals, Inc. Pharmaceutical compositions and treatment methods
US6673771B1 (en) 1999-07-28 2004-01-06 The Trustees Of The University Of Pennsylvania Methods of inhibiting osteoclast activity
US6551608B2 (en) 2000-03-06 2003-04-22 Porex Technologies Corporation Porous plastic media with antiviral or antimicrobial properties and processes for making the same
EP1311531B1 (en) 2000-08-15 2016-05-11 Brigham Young University Steroid derived antibiotics
US6514556B2 (en) 2000-12-15 2003-02-04 Ecolab Inc. Method and composition for washing poultry during processing
US9056048B2 (en) 2001-08-16 2015-06-16 The Trustees Of The University Of Pennsylvania Synthesis and use of cationic steroids for anti-inflammatory drug therapy
ATE468861T1 (de) 2001-08-16 2010-06-15 Univ Pennsylvania Synthese und verwendung von reagenzien für die verbesserte dna-lipofektion und/oder prodrug- und arzneimitteltherapien mit langsamer freisetzung
US6939376B2 (en) 2001-11-05 2005-09-06 Sun Biomedical, Ltd. Drug-delivery endovascular stent and method for treating restenosis
DE10218147B4 (de) 2002-04-23 2005-12-22 Stockhausen Gmbh Wasserabsorbierende, die Zersetzung von Körperflüssigkeiten verzögernde Polymerteilchen, deren Herstellung und Verwendung
US9211259B2 (en) 2002-11-29 2015-12-15 Foamix Pharmaceuticals Ltd. Antibiotic kit and composition and uses thereof
US7659061B2 (en) 2002-11-19 2010-02-09 Pharmacia & Upjohn Company Pharmaceutical compositions having a modified vehicle
US7282214B2 (en) 2002-12-19 2007-10-16 Johnson & Johnson Vision Care, Inc. Biomedical devices with antimicrobial coatings
WO2004112852A1 (en) 2003-06-23 2004-12-29 Beiersdorf Ag Antimicrobial wounddressing
GB0318901D0 (en) 2003-08-12 2003-09-17 Univ Bath Improvements in or relating to bone substitute material
US20050267051A1 (en) * 2003-12-19 2005-12-01 Lee Henry J Antineoplastic and anti-HIV conjugates of steroid acid and nucleosides
US8119154B2 (en) 2004-04-30 2012-02-21 Allergan, Inc. Sustained release intraocular implants and related methods
US20060062773A1 (en) 2004-09-21 2006-03-23 The Procter & Gamble Company Compositions for maintaining and restoring normal gastrointestinal flora
WO2007089903A2 (en) * 2006-02-01 2007-08-09 Brigham Young University Cationic steroid microbial compositions for treating or preventing influenza infections
US20070190067A1 (en) 2006-02-01 2007-08-16 Savage Paul B Cationic Steroid Antimicrobial Compositions and Methods of Use
WO2007089906A2 (en) * 2006-02-01 2007-08-09 Brigham Young University Cationic steroid antimicrobial compositions for treating or preventing herpes infections
WO2008048340A2 (en) * 2006-02-01 2008-04-24 Brigham Young University Cationic steroid antimicrobial compositions for treating poxvirus infections
WO2007134176A2 (en) 2006-05-10 2007-11-22 Microban Products Company Antimicrobial food pad
US7858000B2 (en) 2006-06-08 2010-12-28 Novartis Ag Method of making silicone hydrogel contact lenses
US8685421B2 (en) 2006-07-07 2014-04-01 Surmodics, Inc. Beaded wound spacer device
KR100950204B1 (ko) * 2006-09-26 2010-03-29 경북대학교 산학협력단 7α-아미노스테로이드 유도체 또는 이의 약학적으로허용가능한 염, 이의 제조방법 및 이를 유효성분으로함유하는 항암용 또는 항생제 조성물
US7854941B2 (en) 2007-02-12 2010-12-21 The University Of Southern Mississippi Method of attaching drug compounds to non-reactive polymer surfaces
US20090016973A1 (en) 2007-07-09 2009-01-15 Micropure, Inc. Composition and Method for the Prevention of Oral Disease
US20090068122A1 (en) 2007-09-06 2009-03-12 Shira Pilch Dentifrice Compositions for Treating Xerostomia
US9095568B2 (en) 2007-09-26 2015-08-04 Mark Berninger Therapeutic and vaccine polyelectrolyte nanoparticle compositions
EP2303026B1 (en) 2008-06-17 2020-09-09 Brigham Young University Cationic steroid antimicrobial diagnostic, detection, screening and imaging methods
US20090324517A1 (en) 2008-06-30 2009-12-31 Dan Kline Halitosis treatment
CN102480969A (zh) * 2009-07-09 2012-05-30 科锐医疗有限公司 伤口愈合和瘢痕调制方法
US8623416B2 (en) 2009-11-25 2014-01-07 Michael Zasloff Formulations comprising aminosterols
US8529681B1 (en) * 2010-01-07 2013-09-10 Sandia Corporation Biofouling-resistant ceragenin-modified materials and structures for water treatment
US9180132B2 (en) 2010-03-04 2015-11-10 The Trustees Of The University Of Pennsylvania Antimicrobial cationic steroids and methods of use
US8871716B2 (en) * 2010-10-08 2014-10-28 Korea Advanced Institute Of Science And Technology Use of antimicrobial peptides in regeneration of skin cells
RU2576459C2 (ru) 2010-11-03 2016-03-10 Брайем Янг Юниверсити Устойчивые при хранении антимикробные композиции, включающие церагениновые соединения, и способы их применения
CN102172356B (zh) * 2011-03-03 2013-04-03 山东农业大学 牛磺鹅去氧胆酸在制备防治骨质疏松的药物中的应用
US8810380B2 (en) 2011-07-01 2014-08-19 Gerald F. Leitz Golf scoring management system
MX340644B (es) 2011-07-20 2016-07-19 Univ Brigham Young Compuestos de ceragenina hidrófobos y dispositivos que incorporan los mismos.
US8945217B2 (en) 2011-08-25 2015-02-03 Brigham Young University Medical devices incorporating ceragenin-containing composites
WO2013029059A1 (en) 2011-08-25 2013-02-28 Brigham Young University Medical devices incorporating ceragenin-containing composites
WO2013029055A1 (en) 2011-08-25 2013-02-28 Brigham Young University Incorporation of particulate ceragenins in polymers
AU2011385377B2 (en) 2011-12-21 2017-06-01 Brigham Young University Oral care compositions
US20130236619A1 (en) 2012-03-01 2013-09-12 Brigham Young University Anti-microbial food processing compositions including ceragenin compounds and methods of use
US20130280391A1 (en) 2012-04-24 2013-10-24 Brigham Young University Anti-microbial wash compositions including ceragenin compounds and methods of use for treating non-meat food products
NZ707003A (en) 2012-10-17 2016-04-29 Univ Brigham Young Treatment and prevention of mastitis
KR102203375B1 (ko) 2013-01-07 2021-01-15 브라이엄 영 유니버시티 세포 증식을 감소시키는 방법 및 소정의 질환을 치료하는 방법
CN105209465A (zh) 2013-03-15 2015-12-30 布莱阿姆青年大学 治疗炎症、自身免疫性疾病和疼痛的方法
US9387215B2 (en) 2013-04-22 2016-07-12 Brigham Young University Animal feed including cationic cholesterol additive and related methods

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US428800A (en) * 1890-05-27 miller
US6767904B2 (en) * 1998-03-06 2004-07-27 Bringham Young University Steroid derived antibiotics

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Robert Bucki et al. Resistance of the antibacterial agent ceragenin CSA-13 to inactivation by DNA or F-actin and its activity in cystic fibrosis sputum / Journal of Antimicrobial Chemotherapy, 2007, V.60, pp.535-545. *
S. Guo et al. Factors Affecting Wound Healing / Journal of Dental Research, 2010, V.89, N.3, pages 219-229. *
S. Guo et al. Factors Affecting Wound Healing / Journal of Dental Research, 2010, V.89, N.3, pages 219-229. Robert Bucki et al. Resistance of the antibacterial agent ceragenin CSA-13 to inactivation by DNA or F-actin and its activity in cystic fibrosis sputum / Journal of Antimicrobial Chemotherapy, 2007, V.60, pp.535-545. *

Also Published As

Publication number Publication date
US20130243823A1 (en) 2013-09-19
CA2848567C (en) 2020-03-24
AU2012308530A1 (en) 2014-04-03
KR101966293B1 (ko) 2019-04-08
IL231509B (en) 2019-02-28
CN104080458A (zh) 2014-10-01
EP2760454B1 (en) 2018-08-29
AU2012308530B2 (en) 2016-04-21
BR112014005914A2 (pt) 2017-06-13
BR112014005914B1 (pt) 2021-11-09
MX369385B (es) 2019-11-07
US9161942B2 (en) 2015-10-20
EP2760454A1 (en) 2014-08-06
JP6038155B2 (ja) 2016-12-07
WO2013040265A1 (en) 2013-03-21
CA2848567A1 (en) 2013-03-21
IL231509A0 (en) 2014-04-30
RU2014112784A (ru) 2015-10-20
AU2012308526B2 (en) 2016-04-21
AU2012308526A1 (en) 2014-04-03
MX2014003020A (es) 2014-10-14
JP2014530192A (ja) 2014-11-17
KR20140075729A (ko) 2014-06-19
WO2013040269A1 (en) 2013-03-21
CN104080458B (zh) 2020-06-02

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