RU2471493C2 - Biogenic stimulator and method for preparing it - Google Patents

Abstract

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutics. A method for preparing a biogenic stimulator consisting in the fact that grown male bees are caught by a trap, kept in a cold chamber with grinding in a sterile laboratory mill; the prepared biologically active mass is diluted in 0.9% sodium chloride; the mixture is autoclaved, filtered, added with 0.9% sodium chloride, packed, hermetically sealed under certain conditions. The biogenic stimulator prepared by the method described above contains the biologically active mass made of male bees and 0.9% sodium chloride.

EFFECT: biogenic stimulator prepared by the declared method provides higher resistance and production qualities of farm animals.

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Description

The invention relates to veterinary medicine, in particular to pharmaceutical preparations for increasing the general and specific resistance of an organism.

Known biostimulant-placenta, the active principle of the placenta of women. The preparation is made according to a new technology, without heat treatment using the cavitation effect and newly discovered chemicals when sterilizing (see. A new generation of tissue preparations - PAN // "Theoretical and practical aspects of the emergence and development of animal diseases and the protection of their health in modern conditions." International conference. Voronezh, October 3-4, 2002. Materials of the conference. - Volume 1. - Voronezh, 2003. - 295 p.).

The disadvantage of this method of preparing a biostimulant is the high cost, complexity and multi-stage process of its preparation.

The closest in technical essence and the achieved positive effect to the claimed biogenic stimulant and the method of its manufacture is the "Method of manufacturing a biogenic stimulator from bee drone brood larvae" according to Russian patent No. 2395289, IPC7 A61K 35/64, which includes 50% of the biologically active mass from drones , 49.7% sodium chloride solution and 0.3% preservative. To prepare the preparation, honeycombs with live healthy drone brood aged 18-22 days are kept in the refrigerator at a temperature of 3-4 ° C for 5-6 days, then brood honeycombs are opened and drones of the same size, light gray in color, are selected and then drones larvae are crushed in a sterile laboratory mill, diluted with a sterile solution of sodium chloride with a concentration of 0.9% in a ratio of 1: 1 and autoclaved at a temperature inside the boiler of 120 ° C and a steam pressure in a jacket of 1.5 atmospheres for 1 h, then the mass is filtered off what After 2 layers of gauze in a sterile volumetric dish, bring with a sterile solution of sodium chloride with a concentration of 0.9% to the original volume, add a preservative (phenol), pour, subject to aseptic and antiseptic rules, into prepared sterile bottles and seal them tightly, autoclave at 120 ° C and steam pressure in the jacket 1.5 atmospheres for 20 minutes

The disadvantage of this method is the high cost of materials, the complexity and multi-stage process of its manufacture.

The aim of the invention is to reduce the cost of biogenic stimulant and simplify the method of its manufacture.

This goal is achieved by the fact that the method of manufacturing a biogenic stimulant, namely, that the drones of the bees are kept in a refrigerator, followed by grinding in a sterile laboratory mill to obtain a biologically active mass, which is diluted with a sterile solution of sodium chloride 0.9% in a ratio of 1: 1 the mixture is autoclaved at a temperature of 120 ° C for one hour, then it is filtered off, 0.9% sodium chloride solution is added, then it is packaged, hermetically sealed and autoclaved at a temperature of 12 0 ° C for 20 minutes, and adult drones are used as drone bees, which are caught in bee hives by means of a drone catcher, kept in a refrigerator for 48 hours at a temperature of 2-4 ° C, the mixture is autoclaved at a pressure of 0.4 MPa, and the amount of 0.9% sodium chloride solution is taken at the rate of 75-85% of the total volume of the stimulator.

The biogenic stimulator itself contains a biologically active mass obtained from drones of bees and a sodium chloride solution of 0.9%, the stimulator obtained by the method described above.

Biogenic stimulant is made as follows. First, adult drones are caught in beehives by means of a drone catcher, then they are kept in a refrigerator for 48 hours at a temperature of 2-4 ° C. When the drones are kept in the refrigerator, they gradually die, but in the process of biochemical reconstruction, biogenic substances are produced in their organisms, which are biological stimulants. The dead drones are crushed in a sterile laboratory mill, then diluted with crushed drones with a sterile solution of 0.9% sodium chloride in a 1: 1 ratio, the mixture is autoclaved at a temperature of 120 ° C and a pressure of 0.4 MPa for one hour, then it is filtered off, first through two layers of gauze, then through a paper filter. A solution of sodium chloride is added to the filtered mass at the rate of 80% of the total volume of the stimulant, then packaged, hermetically sealed and sterilized in an autoclave at a temperature of 120 ° C and a pressure of 0.4 MPa for 20 minutes.

The method can significantly reduce the manufacturing time of the drug, reduce its cost and simplify the method of its manufacture.

A biogenic stimulant made in this way helps to increase the growth and development of young farm animals, their reproductive ability, reduce feed costs per unit of increase in live weight, and increase the general and specific resistance of the body.

The activity of a biogenic stimulant is ensured by a wide range of biologically active substances, amino acids, vitamins, enzymes, hormones, macro- and microelements, higher fatty acids, heterocyclic compounds, etc.

As a result of scientific and industrial experience, it was found that the claimed biogenic stimulator has a pronounced growth-promoting effect and provides an increase in average daily gain in live weight of piglets during the growing period by 51.8 grams and body weight by three months of age by 3.23 kilograms compared with the control group . The most effective is a triple injection of a biogenic stimulator after 7 days at a dose of 0.1 ml per 1 kg of live weight.

Biogenic stimulant has a pronounced immunostimulating effect, consisting in the correction of the body's immune system at the level of the humoral and non-specific links. When using this stimulant, weaned pigs significantly increase the amount of hemoglobin, red blood cells, white blood cells, reserve alkalinity, total protein content, alpha and gamma globulins, lysozyme and bactericidal activity in the blood and its serum.

The use of the inventive biogenic stimulator will reduce the cost of materials for its manufacture, as well as simplify the process of its manufacture.

Claims (2)

1. A method of manufacturing a biogenic stimulant, namely, that the drones of bees are kept in a refrigerator, followed by grinding in a sterile laboratory mill to obtain a biologically active mass, which is diluted with a sterile sodium chloride solution of 0.9% in a ratio of 1: 1, the mixture is autoclaved at at a temperature of 120 ° C for one hour, then it is filtered off, a sodium chloride solution of 0.9% is added, then it is packaged, hermetically sealed and autoclaved at a temperature of 120 ° C for 20 minutes, characterized by m, that adult drones are used as drone bees, which are caught in bee hives by means of a drone catcher, kept in a refrigerator for 48 hours at a temperature of 2-4 ° C, the mixture is autoclaved at a pressure of 0.4 MPa, and the amount of sodium chloride solution is 0, 9% take from the calculation of 75-85% of the total volume of the stimulator. 2. Biogenic stimulant containing a biologically active mass obtained from drones of bees, and a sodium chloride solution of 0.9%, characterized in that the stimulant is obtained by the method according to claim 1.