RU2561863C1 - Method for producing biological stimulator - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention represents a method for producing a biological stimulator containing 50% of biologically active mass of male bee larvae, 49.7% of sodium chloride and 0.3& of a preserving agent consisting in the fact that the preparation is prepared by keeping bee's cells with alive 3-5-day-old male bee brood in a fridge at temperature 3-4°C for 5-6 days, opening the cells with the brood and selecting the male bee larvae of the same size in light-grey, grinding the male bee larvae in a sterile laboratory mill, diluted with 0.9% saline in ratio 1:1 and autoclaved at inner curing temperature 120°C and jacket steam pressure 1.5 atm for 1 hour, filtering the mass through 2 gauze layers into a sterile graduated bottle, reducing to initial volume with sterile 0.9% saline, adding with the preserving agent phenol, bottling into the prepared sterile flasks while observing the aseptic and antiseptic regulations, sealing the flasks tightly, autoclaved at 120°C and jacket steam pressure 1.5 atm for 20 minutes.

EFFECT: invention promotes enhancing the growth and development function in animals, increasing the safety of animal yield and improving the production qualities in the animals.

3 ex, 3 tbl

Description

The invention relates to veterinary medicine, in particular to preparations for improving the productive qualities of farm animals.

Prior art study

A known method of obtaining a biostimulating agent based on honey, including its mechanical mixing with other bee products and medicinal plants, moreover, per 100 kg of monofleur honey from white acacia flowers add 15 kg of royal jelly, 110 kg of hemolymph extract of bees, 0.1-0.2 kg of drones seed and 110 kg of white acacia juice, vetch, alfalfa, oregano and thyme, then the resulting mass is thoroughly mixed and fed to bees in the absence of a bribe, and the evacuated honey is used as a finished product (RF Patent No. 2085199, cl. A61K 35/64, 1991).

The disadvantage of this invention is the narrow scope of the product obtained by this method, as well as the fact that the composition of the product contains royal jelly, which is a product of immediate use, because under the influence of external factors, such as sunlight, heat, oxidation of atmospheric oxygen, its therapeutic properties significantly reduced.

There is also known a method of preparing a biologically active food supplement of general strengthening action "Khan-Bala", which involves extracting drone brood larvae from 4-6 days old from bee honeycombs, dispersing and mixing the resulting homogenate with honey in the following ratio of components, wt.%: Honey - 70 -80; Homogenate of drone larvae - 20-30.

(Patent of the Republic of Kazakhstan No. 41505, MKI A23L 1/08, Authors: Ekimov B.P. (RU), Yankova S.F. (RK), Nenasheva N.F. (RK)).

The disadvantage of this method is the long duration of the intake of dietary supplements to obtain a positive effect due to the oral administration of the drug, its biologically active substances have little effect on the growth function of the stimulated object and do not provide an increase in the productive qualities of farm animals.

Closest to the proposed technical solution is the well-known Method for the preparation of a biogenic stimulator, including 50% biologically active mass from drones larvae, 49.7% sodium chloride solution and 0.3% preservative, characterized in that the honeycomb with live healthy drone brood is 18- 22 days are kept in the refrigerator at a temperature of 3-4 ° C for 5-6 days, then the brood honeycombs are opened and drones larvae of the same size, light gray in color are selected, after which drones larvae are crushed in a sterile laboratory mill, diluted with a sterile solution of sodium chloride with a concentration of 0.9% in a ratio of 1: 1 and autoclaved at a temperature inside the boiler of 120 ° C and a steam pressure in the jacket of 1.5 atmospheres for 1 h, then the mass is filtered through 2 layers of gauze in sterile volumetric utensils, adjusted with a sterile sodium chloride solution with a concentration of 0.9% to the original volume, add a preservative (phenol), pour, subject to aseptic and antiseptic rules, into prepared sterile vials and seal them tightly, autoclave at 120 ° C and pressurized and a couple in a 1.5 atmosphere shirt for 20 minutes (RF Patent No. 2395289, A61K 35/64, 2008 Author (s): Pogodaev Vladimir Anikeevich (RU), Klimenko Aleksandr Ivanovich (RU), 3ubenko Aleksandr Aleksandrovich (RU), Fetisov Leonid Nikolaevich (RU), Klimenko Vladimir Alexandrovich (RU), Pogodaev Alexey Vladimirovich (RU)).

The disadvantage of this method is that the drone brood at the age of 18-22 days is a pupa, and its biologically active substances have little effect on the growth function of the stimulated object and provide a slight increase in the productive qualities of farm animals.

The objective of the invention is to change the properties of a biological stimulator in order to enhance the growth function of a stimulated object.

The technical result is an enhanced growth function of a stimulated object and an active increase in the productive qualities of farm animals.

The technical result is achieved by the fact that in the method of preparing a biological stimulant, comprising 50% of the biologically active mass from drones larvae, 49.7% sodium chloride solution and 0.3% preservative, honeycombs with live healthy drone brood 3-5 years old are used to prepare the preparation days, which are kept in the refrigerator at a temperature of 3-4 ° C for 5-6 days, then the brood honeycombs are opened and drones of the same size, light gray in color, are selected and then the drones are crushed in a sterile laboratory Oh mill, diluted with a sterile solution of sodium chloride with a concentration of 0.9% in a ratio of 1: 1 and autoclaved at a temperature inside the boiler of 120 ° C and a steam pressure in the jacket of 1.5 atmospheres for 1 hour, then the mass is filtered through 2 layers of gauze in sterile volumetric utensils, adjusted with a sterile sodium chloride solution with a concentration of 0.9% to the original volume, add a preservative (phenol), poured, subject to aseptic and antiseptic rules, into prepared sterile vials and sealed them, autoclaved at 120 ° C and pressure uu steam in the jacket of 1.5 atmosphere for 20 minutes. The method can significantly reduce the time of manufacture of the drug, increase its safety, increase shelf life. Biological stimulant obtained in this way, enhances the growth function and development of animals.

An essential distinguishing feature of the proposed method is the following action: the use of larvae of drone brood 3-5 days of age.

The proposed method is as follows. A biological stimulant, including 50% of the biologically active mass from drones larvae, 49.7% sodium chloride solution and 0.3% preservative, honeycombs with a healthy drone brood 3-5 years old are kept from a bee hive, which are kept in the refrigerator at a temperature of 3- 4 ° C for 5-6 days, then the brood honeycomb is opened and drones of the same size, light gray in color, are selected, after which the drones are crushed in a sterile laboratory mill, diluted with a sterile solution of sodium chloride with a concentration of 0.9% in proportion and 1: 1 and autoclave at a temperature inside the boiler of 120 ° C and a steam pressure in a jacket of 1.5 atmospheres for 1 hour, then the mass is filtered through 2 layers of gauze in a sterile volumetric dish, adjusted with a sterile solution of sodium chloride with a concentration of 0.9% to the original volume, add a preservative (phenol), pour, subject to the rules of asepsis and antiseptics, into prepared sterile bottles and seal them tightly, autoclave at 120 ° C and steam pressure in a 1.5 atmosphere jacket for 20 minutes. The method allows to prepare a biological stimulant, comprising 50% of the biologically active mass of drones, 49.7% sodium chloride solution and 0.3% preservative, which helps to enhance the growth and development of animals.

Table 1 shows the results of a comparative analysis of the number of growth-promoting substances of a biological stimulator from the larvae of drone brood bees compared to the protopip.

The invention in comparison with the prototype and other known technical solutions has the following advantages:

- the biostimulant has an increased concentration of methionine, histidine, glutamic acid;

- biostimulant enhances the growth function of the stimulated object by 10%;

- provides an active increase in the productive qualities of farm animals.

The implementation of the method is illustrated by the following examples.

Test results of a biogenic stimulator from drone brood in animals

Example 1. In the manufacture of an experimental batch of the drug conducted a biological test for its harmlessness. The test was carried out on laboratory rabbits. When the drug was administered intramuscularly and subcutaneously, an acute toxic reaction in animals was not noted, and the pyrogenic or allergic effects of the drug were not established.

Example 2. Evaluation of the influence of a biological stimulant on the growth and development of animals was carried out on rabbits of the breed gray giant for a period of 2 months. Three groups of young rabbits of 2 months of age were created.

The first group of rabbits was fed according to the standards adopted in rabbit breeding. The content mode is standard.

The second experimental group of rabbits was fed according to the standards adopted in rabbit breeding. Under the standard regimen of maintenance, 4-fold, after 8 days, Biogenic stimulant, RF Patent No. 2395289, at a dose of 0.05 ml / kg was administered.

The third experimental group of rabbits was fed according to the standards adopted in rabbit breeding. Under the standard regimen of maintenance, 4 times, after 8 days, was introduced. The biological stimulator according to the claims at a dose of 0.05 ml / kg.

As shown by experimentally obtained data, throughout the entire growing period, rabbits showed a positive reaction to the introduction of a biological stimulant into the body, they were distinguished by better growth, development, had high live weight and average daily gains (the average daily weight gain in rabbits of the experimental group was 7 times higher 6 g compared to the standard content group and 4.6 g compared to the group to which the stimulant was administered Patent of the Russian Federation No. 2395289). The use of a biostimulant increased the safety of rabbits by 6.6% compared with the control groups.

Example 3. The influence of a biological stimulant on the development of bee colonies. In the experiments, bees of the local population were used.

A biological stimulant was given with food (sugar syrup 60% concentration) at a concentration of 0.005%, 0.01% and 0.05%. Control served the same feed. Feeding was carried out for 12 days. Families were fed 0.2 kg of feed daily. The number of families in each group is 10.

The effect of a biological stimulant on the number of broods in the family (egg laying of the uterus) was evaluated.

In the control group, a decrease in the number of frames occupied by brood was observed, which is consistent with the normal life cycle of the bee family in this period. In the experimental group, the picture is completely opposite - the number of brood increased by 26% compared with the control. This indicates a significant stimulating effect of the biological stimulator on the egg production of the uterus, which allows you to maintain strong families throughout the period of honey collection and, as a result, get more honey from them.

Claims (1)

A method of preparing a biological stimulant comprising 50% of the biologically active mass from drones larvae, 49.7% sodium chloride solution and 0.3% preservative, characterized in that the cells with live healthy drone brood aged 3-5 days are kept in the refrigerator at a temperature of 3 -4 ° C for 5-6 days, then the brood honeycombs are opened and drones of the same size, light gray in color, are selected, after which the drones are crushed in a sterile laboratory mill, diluted with sterile sodium chloride solution with concentrate 0.9% in a ratio of 1: 1 and autoclave at a temperature inside the boiler of 120 ° C and a steam pressure in the jacket of 1.5 atmospheres for 1 h, then the mass is filtered through 2 layers of gauze in a sterile volumetric dish, adjusted with a sterile solution of sodium chloride with a concentration of 0.9% to the initial volume, phenol is added to the preservative, poured in compliance with the rules of asepsis and antiseptics into prepared sterile vials and hermetically sealed, autoclaved at 120 ° C and a steam pressure in the jacket of 1.5 atmospheres for 20 minutes.