RU2258522C1 - Method for manufacturing a preparation for stimulating animal bodies out of drone bee larvae - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: the present innovation deals with medicinal preparations for increasing total and specific stimulation of animal body. The suggested preparation should be obtained out of drone bee larvae of different age. From the cells of drone bee brood one should cut off convex wax caps with a special knife for opening honeycombs, then due to light knocking one should remove larvae out of the cells into a cuvette to be put into vials. Larvae should be frozen and homogenized, moreover, as a stabilizing medium one should apply 5%-glucose solution at 1:10 ratio at addition of 5%-chloroform solution or 2%-salicylic acid followed by sublimation to be finally poured into vials per 5 ml. Moreover, sublimation should be fulfilled in vacuum chamber at temperature of shelves' heating up to 30-45 C. At achieving preparation temperature of 20-22 C shelves' temperature should be decreased up to 25 C to perform final heating for 22-24 h. The method provides increased total and specific stimulation of animal body due to applying cheaper and more available biostimulant.

EFFECT: more simplified method of manufacturing.

3 ex

Description

The technical field to which the invention relates.

The invention relates to veterinary medicine, in particular to pharmaceutical preparations for increasing the general and specific stimulation of an animal organism.

State of the art

A known method of preparing tissue suspensions from parenchymal organs (liver, spleen, testes, kidneys, etc.), which are easy to process. After five days of preservation at a temperature of 2-4 ° C, the tissue is washed with boiled water, weighed, crushed in a sterile homogenizer and additionally triturated in a porcelain mortar-homogenizer with the gradual addition of physiological saline (2-3 ml of solution per 1 g of tissue). The mass thus prepared is left for two hours at room temperature, and then placed in a water bath at 60-80 ° C for 30 minutes. After that, the mass is filtered through 2-3 layers of sterile gauze and the filtrate is poured into ampoules or vials. The ampoules are sealed, and the vials are closed with stoppers and autoclaved for 60 minutes at 120 °. Then the vials are poured with paraffin or sealing wax, glued labels (see I.A. Kalashnik. Tissue therapy in veterinary medicine. M: Selkhozgiz, 1960, p.22).

The disadvantage of this method is the high cost of materials, complexity and multi-stage.

A known method of preparing a biostimulant for feeding birds, which includes introducing into the main diet a biostimulant prepared by cooling at a temperature of 2-4 ° C for 4-5 days, diluting with water in a five-fold volume and cooling for 24 hours, followed by autoclaving the resulting mass. In this case, egg mass is used as a biostimulant and, after processing, it is introduced into the diet in an amount of 0.2-0.3 ml per 1 kg of live weight with an interval of 6-7 days (see AS USSR No. 1530163, class. A 23 K 1/16).

The disadvantage of this method is the insufficiently high effect of increasing the general and specific resistance of the organism.

The closest in technical essence and the achieved positive effect and adopted by the authors for the prototype is a method of preparing a preparation for stimulating the human body from drones, including collecting drones, their homogenization with the addition of a stabilizing medium (see. "Harvesting of drones", V.I. Lebedev, M.A. Legovich, Research Institute of Beekeeping, J. Beekeeping, No. 3, 2003, p.52-54).

The disadvantage of this method is the short storage time of the drug.

Disclosure of invention

The technical result that can be achieved using the proposed method is to increase the general and specific stimulation of the animal organism, to obtain a cheaper and more affordable biostimulator, to simplify the preparation method.

The technical result is achieved using the method of preparation of the drug to stimulate the body of animals from drones larvae, including collection of larvae, their homogenization with the addition of a stabilizing medium, and before homogenization, the darling larvae are frozen at a temperature of (-4; -5 ° C) for 12-14 hours, then thawed to a temperature of + 18 ° C and homogenized for 3-5 minutes, while a stabilizing medium using a 5% glucose solution in a ratio of 1:10 with the addition of a 5% solution of chloroform or 2% salicylic acid with the next sublimation, and sublimation is carried out in a vacuum chamber at a temperature of shelf heating to 30 ° C-45 ° C, while when the drug reaches a temperature of 20 ° C-22 ° C, the temperature of the shelves is reduced to 25 ° C and drying is carried out for 22-24 hours.

The essence of the method of preparation of the drug to stimulate the body of animals from drone larvae

The drug is made from drones larvae of various ages. For this purpose, convex wax caps are cut from drone brood cells using a knife for printing honeycombs. Then, with light tapping, their larvae are knocked out of the cells on a clean cuvette (baking sheet), collected in a container (50–200 ml), then drones larvae are frozen at a temperature of (-4; -5 ° C) for 12-14 hours, then thawed to a temperature of + 18 ° C and homogenized for 3-5 minutes, while a stabilizing medium using a 5% glucose solution in a ratio of 1:10 with the addition of a 5% solution of chloroform or 2% salicylic acid, followed by sublimation, poured into vials of 5 ml, and sublimation is carried out in a vacuum chamber at temperatures e heating the shelves to 30 ° С-45 ° С, while when the temperature of the preparation reaches 20 ° С-22 ° С, the temperature of the shelves is reduced to 25 ° С and drying is done for 22-24 hours.

The implementation of the invention

Examples of specific experiments on the preparation of the drug to stimulate the body of animals from drones larvae.

Experience No. 1. Convex wax caps are cut from the cells of the drone brood, then their larvae are knocked out of the cells onto a clean cuvette (pan) with a light tapping, and collected in containers (50-200 ml in volume). In this case, the refrigerator with the tested, for example, 9-shelf rack loaded in it is pre-cooled to -20 ° C, the cassettes with the drug are installed on the shelves of the shelf, the temperature sensors of the drug are installed in the cassettes brought from packaging last. After loading is complete, the freezing temperature is set (-30 ° C) and the refrigerator is turned on, freezing is carried out for 5-10 hours, then the preparation is thawed to a temperature of + 18 ° C and homogenized for 1-2 minutes, while using as a stabilizing medium 5% glucose solution in a ratio of 1:10 with the addition of a 5% chloroform solution followed by sublimation, moreover, sublimation is carried out in a vacuum chamber at a temperature of heating the shelves to (20 ° C-25 ° C), while reaching a temperature of the drug 15-18 ° C, the temperature is reduced to 15 ° C and rovodyat final drying for 15-20 hours.

The resulting preparation does not withstand the specified shelf life of 2 years, when used by animals it does not give a high effect of increasing the general and specific stimulation of the body.

Experience No. 2. Carried out similarly to experiment No. 1, but freezing is carried out at a temperature of (-4; -5 ° C) for 12-14 hours, then thawed to a temperature of + 18 ° C and homogenized for 3-5 minutes, while as a stabilizing medium use a 5% glucose solution in a ratio of 1:10 with the addition of 2% salicylic acid, followed by sublimation, pour into 5 ml vials, and sublimation is carried out in a vacuum chamber at a shelf heating temperature of up to 30 ° C-45 ° C, while when the temperature of the preparation reaches 20 ° С-22 ° С, the temperature of the shelves is reduced to 25 ° С and drying with within 22-24 hours.

The resulting preparation is a loose, light brown mass, from which tablets are prepared that are easily soluble in sterile physiological saline or distilled water, which is administered intramuscularly or given inside the animals.

The resulting preparation with these parameters can withstand the specified shelf life of 2 years and when used by animals, such as pigs, it boosted the immune system and increased weight, and the mink has litter.

Experience No. 3. Carried out similarly to experiment No. 1, but freezing is carried out at a temperature (-5 ° C) for 15-20 hours, followed by thawing to a temperature of + 18 ° C and homogenization for 6-10 minutes. A 5% glucose solution in a ratio of 1:10 with the addition of a 5% solution of chloroform or 2% salicylic acid followed by sublimation is used as a stabilizing medium, moreover, sublimation is carried out in a vacuum chamber at a temperature of the shelves heated to 46 ° C-55 ° C, when the temperature of the preparation reaches 35 ° С-40 ° С, the temperature of the shelves is reduced to 30 ° С and the drying is carried out for 25-30 hours.

The obtained preparation with these parameters turned out to be too friable and could not be packaged in tablets, could not withstand the specified shelf life; when fed to animals, it did not give a high effect of increasing the general and specific stimulation of the body.

The invention in comparison with the prototype and other known technical solutions has the following advantages:

- the drug has the effect of tissue therapy;

- increases the general and specific stimulation of the body;

- simplified in the manufacturing method;

- cheaper to manufacture;

- the drug can be administered s / c, in / m and inside;

- has a shelf life of 2 years.

Claims (1)

A method of preparing a preparation for stimulating an animal organism from drones larvae, including collecting drones larvae, homogenizing them with the addition of a stabilizing medium, characterized in that before homogenizing drones, the drones are frozen at a temperature of (-4; -5 ° C) for 12-14 hours , then thawed for 3-5 min to + 18 ° C, while a stabilizing medium using a 5% glucose solution in a ratio of 1:10 with the addition of a 5% solution of chloroform or 2% salicylic acid, followed by sublimation, and sublimation about lead in a vacuum chamber at a temperature of heating the shelves to (+ 30 ° C) - (+ 45 ° C), while reaching the temperature of the drug (+ 20 ° C) - (+ 22 ° C) the temperature of the shelves is reduced to + 25 ° C and produce drying for 22-24 hours