RU2197251C2 - Method for preparing an embryonic biostimulator - Google Patents

Abstract

FIELD: veterinary science. SUBSTANCE: the innovation deals with medicinal preparations to increase total and specific body resistance. One should select fresh, uninfected hen eggs to incubate them for 9 d, moreover, on the 5th, 6th, and 7th d eggs are irradiated per 30 sec at modulation frequency of 9 Hz and radiation power of 9 mW, then eggs should be cooled for 6-7 d, homogenization is conducted, egg mass is diluted with physiological solution at 1:10 ratio, mixed thoroughly, then the mixture obtained is supplemented with a 5%-phenolic solution to obtain phenol concentration of 0.3%, the obtained solution is filtered and poured into sterile vials, covered and kept in a refrigerator at 2-6 C. The method enables to increase total and specific body resistance, obtain cheaper and more available biostimulator and more simplified process of preparation. EFFECT: higher efficiency.

Description

 The invention relates to veterinary medicine, in particular to pharmaceutical preparations for increasing the general and specific resistance of an organism. Embryonic biostimulant (STEMB) can be used by subcutaneous administration to agricultural pets.

 Known biostimulant - the placenta, the active principle of the placenta of women. The preparation is made according to a new technology without heat treatment using the cavitation effect and newly discovered chemical reagents during sterilization (see New Generation of Tissue Preparations - PAN // "Theoretical and Practical Aspects of the Emergence and Development of Animal Diseases and the Protection of Their Health in Modern Conditions." International Conference Voronezh, October 3-4, 2000. Conference proceedings. - Volume 1. - Voronezh, 2000-295 p.).

 The disadvantage of this method of preparing a biostimulant is the high cost, complexity and multi-stage preparation.

There is a method of preparing tissue suspensions from parenchymal organs (liver, spleen, testes, kidneys, adrenal glands, etc.), which are easy to process. After five days of preservation at a temperature of 2-4 ^o C, the tissue is washed with boiled water, weighed, crushed in a sterile meat grinder and additionally triturated in a porcelain mortar - homogenizer with the gradual addition of physiological saline (2-3 ml of solution per 1 g of tissue). The mass thus prepared is left for two hours at room temperature, and then placed for half an hour in a water bath at 60-80 ^o C. After that, the mass is filtered through 2-3 layers of sterile gauze and the filtrate is poured into ampoules and vials. The ampoules are sealed, and the vials are closed with corks and autoclaved for 1 hour at 120 ^o С, then the vials are filled with paraffin or wax, and labels are glued (see "Fabric Therapy in Veterinary Medicine" I.A. Kalashnik, M .: Selkhozgiz, 1960, p. 22 )

 The disadvantage of this method is the high cost of materials, complexity and multi-stage.

A known method of preparing a biologically active suspension of bones, which canned at a temperature of 2-4 ^o C for four days. Then the bones are autoclaved at 4 atm for 3 hours, after which they are dried in an oven at a temperature of 70-100 ^o C for two days, crushed in a ball mill and sieved through a thick sieve. The resulting bone meal is thus mixed with a 0.2% agar-agar solution in a ratio of 1: 2 and poured into bottles with ground stoppers and autoclaved for 1 hour at 120 ^o C (see Technique for the preparation of tissue preparations // Stimulating therapy in veterinary medicine I. A. Kalashnik. - 2nd ed., revised and supplemented - K .: Urozhay, 1990. - P.66-67).

 The disadvantages of this method are the high cost of materials, complexity and multi-stage.

A known method of preparing a biostimulant from various tissues and blood and placed in a refrigerator at a temperature of 2-4 ^o With for 7 days. Then the fabric is transferred to a vacuum at a temperature of 60-70 ^o C, dried, passed through a ball mill, the resulting powder is weighed 5 g each and poured into test tubes. The tubes are placed in an autoclave and sterilized at a temperature of 120 ^o C and at a pressure of 1.5 ATM for 1 hour. Then the tubes are closed with rubber stoppers. Outside the tubes are poured with a mixture of wax and paraffin (see Methods of preservation, implantation and tissue injection // Pathogenetic therapy of animal diseases K.I.Shakalov. - L. - M.: Selkhozgiz, 1961. - S.304-310.).

 The disadvantages of this method are the high cost of materials, complexity and multi-stage.

The closest in technical essence and the achieved positive effect and adopted by the authors for the prototype is a method of preparing a biostimulator for feeding birds, which includes introducing into the main diet a biostimulator prepared by cooling at a temperature of 2-4 ^o C for 4-5 days, diluting with water five times volume and sedimentation during the day, followed by autoclaving the resulting mass, while the egg mass is used as a biostimulant and after processing it is introduced into the diet in an amount e 0.2-0.3 ml per 1 kg body weight at intervals of 6-7 days (see. AS USSR 1530163, cl. A 23 K 1/16).

 The disadvantage of this method of preparing a biostimulant for feeding birds is the insufficiently high effect of increasing the general and specific resistance of the body.

 The technical result that can be achieved using the proposed method is to increase the general and specific resistance of the body, to obtain a cheaper and more affordable biostimulator, to simplify the method of preparation.

The technical result is achieved using a known method of preparing an embryonic biostimulator, including egg homogenization, cooling at a temperature of 2-4 ^o C, dilution, filtration, while before homogenizing the egg is incubated for 9 days, and on the 5, 6, 7 days the egg is irradiated in for 30 s at a modulation frequency of 9 Hz and a radiation power of 9 mW (laser apparatus AL-01 "Semicon") and cooled for 6-7 days at a temperature of 2-4 ^o C, while physiological saline in a ratio of 1 is used as a diluent : 10 s add leniem 5% phenol solution to a concentration of 0.3%.

 The essence of the method of preparation of the embryonic biostimulator is as follows.

Fresh, uninfected chicken eggs are selected, then they are incubated for 9 days, while on the 5, 6, 7 days the eggs are irradiated for 30 s at a modulation frequency of 9 Hz and a radiation power of 9 mW, then the eggs are cooled for 6-7 days, homogenization is carried out, the egg mass is diluted 1:10 with physiological solution, mixed thoroughly, a 5% phenol solution is added to the resulting mixture so that the phenol concentration is 0.3%, the resulting solution is filtered and poured into sterile vials, closed and stored in the refrigerator at a temperature of 2-6 ^o C .

 Examples of specific experiments on the preparation of an embryonic biostimulator.

Experience 1. A selection of fresh, non-infected chicken eggs is carried out, then they are incubated for 9 days, homogenized to a homogeneous mass, the egg mass is diluted 1:10 with physiological saline and mixed thoroughly, the resulting mass is placed in a refrigerator for four days at a temperature of 2-4 ^o C.

 The obtained biostimulant has disadvantages: the drug does not withstand the specified shelf life, when used in animals it does not give a high effect of increasing the general and specific resistance of the body, and there are also cases of local inflammatory reactions when administered.

 Experiment 2. Conduct similarly to experiment 1, but during incubation on the 5th, 6th, 7th day, the eggs are irradiated for 30 s at a modulation frequency of 9 Hz and a radiation power of 9 mW.

 The resulting biostimulant does not withstand the specified shelf life, when used by animals it does not give a high effect of increasing the general and specific resistance of the body.

Experiment 3. Carried out similarly to experiments 1, 2, after incubation, the chicken eggs are completely cooled at a temperature of 2-4 ^o C for 6-7 days, and after their homogenization and dilution, a 5% phenol solution is added to the preparation so that the phenol concentration was 0.3%.

The biostimulator obtained with these parameters has a high effect of increasing the general and specific resistance of the body. The invention in comparison with the prototype and other known technical solutions has the following advantages:
- biostimulant obtained using the present invention has the effect of tissue therapy, protein hydrolyzate therapy, organotherapy, nonspecific protein therapy, as well as a bactericidal effect;
- the preparation method allows not only to obtain a drug with the effect of tissue and other types of therapies, but also allows you to preserve the beneficial properties of the egg: lysozyme and bactericidal activity, some vitamins, by irradiation by the 9th day of incubation, achieve high immune parameters of the embryo and activate the metabolic rate;
- cheaper to manufacture;
- The main methods, preparations, devices used in the preparation of the drug are easily accessible.

Claims (1)

A method of preparing an embryonic biostimulator, including the homogenization of an egg cooled at a temperature of 2-4 ^o C, dilution, filtration, characterized in that the egg is incubated for 9 days before homogenization, and the egg is irradiated for 5, 6, 7 days for 30 s at a modulation frequency of 9 Hz and a radiation power of 9 mW and cooled for 6-7 days at a temperature of 2-4 ^o C, and as a diluent, physiological saline is used in a ratio of 1: 10 with the addition of a 5% solution of phenol to its concentration 0 , 3%.