KR102251942B1 - The composition of immune enhancement for beekeeping - Google Patents

Abstract

The present invention relates to an immune booster composition for beekeeping, comprising methyl sulfonyl methane, essential oil, and carnitine as active components. According to the present invention, the immune booster composition for beekeeping can improve the activity and honey-gathering power of bees and increase a survival rate of bees by enhancing the immunity of bees.

Description

Immunostimulant composition for beekeeping {THE COMPOSITION OF IMMUNE ENHANCEMENT FOR BEEKEEPING}

The present invention relates to an adjuvant composition for beekeeping, and more specifically, to an adjuvant composition for beekeeping comprising methyl sulfonyl methane, essential oil, and carnitine as active ingredients.

Beekeeping refers to bees raised to obtain honey or agriculture to raise bees. Beekeeping is widely used because it can produce not only honey, but also beeswax, propolis, pollen, and royal jelly. Since the beekeeping industry occupies a high share not only in Korea but also abroad, it is important to protect bees and maintain the number of bees in the beekeeping industry.

In recent years, the number of bees due to diseases is rapidly decreasing, and diseases of bees are known to be caused by pesticides, herbicides, parasites, fungi, and viruses. In particular, diseases of bees are mainly caused by parasites or pathogens, and representative diseases of bees include nozema disease, buzzer disease, and chalk disease. These diseases of bees break the homeostasis of individual bees and the whole bee colony, and accordingly, it becomes difficult to protect and maintain the number of bees, which negatively affects not only the beekeeping industry but also the agricultural base. Therefore, it is important to enhance the immunity of bees to prevent bee diseases.

The most important thing in the management of beekeeping is to maintain the strength of the beekeeper as a strong force. If the Bonggun is strong, bees can have immunity against diseases, improve watertightness (the ability to collect honey; gathering power), and prevent the verb during wintering in winter. In order to make the Bonggun strong, first of all, spawning in spring must be managed, pesticide damage must be minimized in summer, and the occurrence of viral diseases must be minimized by managing the humidity inside the beehive. In addition, it is very important to reduce the mortality of bees by preventing gangrene disease and controlling mites after harvesting of the major domestic wheat sources such as ash and chestnut honey. However, in spite of satisfying these conditions in most apiaries, there is a limit to enhancing the immunity of bees, and therefore, it is necessary to develop a composition for enhancing the immunity of bees and preventing diseases caused by pests.

Conventionally, a technique for a composition for enhancing the immunity of bees and preventing diseases caused by pests has been known. Specifically, Korean Patent Registration No. 1304073 relates to a composition for controlling bee pests, and proposes a lactic acid fermentation strain as a component capable of effectively eradicating pests such as viras, fungi, and parasites of bees. In addition, Korean Patent No. 2070362 relates to a composition for enhancing immunity of bees, and presents licorice powder and bromelain as immunity enhancing ingredients that can reduce damage caused by diseases without causing resistance and toxicity problems to bees. I'm doing it. However, in the prior art, there is no description of methyl sulfonyl methane, essential oil and carnitine as components for enhancing the immunity of bees.

Accordingly, the present inventors have made intensive research efforts to overcome the problems of the prior art, in the case of a beekeeping adjuvant composition comprising methyl sulfonyl methane, essential oil and carnitine as active ingredients, by enhancing the immunity of the bees And it was confirmed that it can improve the honey-gathering power and increase the survival rate of bees, and completed the present invention.

KR 10-1304073 B KR 10-2070362 B

Accordingly, the main object of the present invention is to provide an immunity enhancing agent composition for beekeeping capable of enhancing the immunity of bees, thereby improving the activity and honey-gathering power of bees and increasing the survival rate of bees.

According to one aspect of the present invention, the present invention provides a beekeeping adjuvant composition comprising methyl sulfonyl methane, essential oil, and carnitine as active ingredients.

In the adjuvant composition for beekeeping of the present invention, the essential oil is characterized in that it comprises at least one oil selected from the group consisting of winter green oil, mir oil, spearmint oil, and lemon glass oil.

Bees are important insects in the beekeeping industry, and it is important to enhance the immunity of bees in order to maintain and increase the population by protecting bees from diseases. Accordingly, the present inventors screened ingredients for increasing the immunity of bees, and found that methyl sulfonyl methane, essential oil and carnitine can enhance the immunity of bees, and thus beekeeping immunity containing the above ingredients as an active ingredient. Invention of the enhancer composition.

The methyl sulfonyl methane is called edible sulfur and is called'organic sulfur compound' or'dietary sulfur', and is known as an ingredient that plays a very important role in making collagen related to skin aging in the body. It is known to be effective in relieving symptoms, preventing interstitial cystitis, and relieving hair loss. When such methyl sulfonyl methane is included in the adjuvant composition for beekeeping of the present invention, it is possible to control larvae, thereby preventing diseases caused by larvae, and enhancing immunity.

The essential oil may be any essential oil generally used in beekeeping compositions, but red thyme oil, tea tree oil, cinnamon oil, which can enhance immunity, Peppermint oil, L-limonene oil, thymol oil, winter green oil, Myrrh oil, spearmint oil and lemon glass It is preferable to use one or more oils selected from the group consisting of oil (lemongrass oil), more preferably winter green oil (Winter green oil), myrh oil (Myrrh oil), spearmint oil (spearmint oil) and lemon glass oil It is preferable to use one or more oils selected from the group consisting of (lemongrass oil). Wintergreen oil is an oil extracted from the leaves of the rhododendron family Gaultheria procumbens, and is known to be used as a raw material for anti-inflammatory pain relievers or has special effects against arthritis or rheumatism. Mir oil is an oil extracted from the resin of the myrrh tree, and is known to be effective against cracked skin and eczema due to its excellent anti-inflammatory and wound healing effects, and useful in relieving respiratory tract infection symptoms. Spearmint oil is an oil extracted by steam distillation of the pollen tops of spearmint plants, and is known to help relieve stress and tension, work usefully in respiratory diseases, and have anti-inflammatory and mucus removal effects. Lemon glass oil is an oil extracted by steam distillation of lemon glass leaves or dried leaves, and is known to have excellent sterilization effects, promote digestion, and have excellent effects in preventing skin aging and improving wrinkles. As described above, the oils are known to have various effects on humans, but the effect of enhancing the immunity of bees is not yet known, and when included in the beekeeping immunity adjuvant composition of the present invention, immunity can be enhanced.

The carnitine may be D-carnitine, L-carnitine, or both D-carnitine and L-carnitine, but it is most preferable to use L-carnitine. Carnitine is a type of amino acid that plays a necessary role in transferring fatty acids to mitochondria in the metabolic process of animals, and is used as a dietary supplement because it aids in the burning of fat, helps to improve brain and kidney functions, and treats male infertility. It is known to be effective in improving blood sugar levels. When such carnitine is included in the adjuvant composition for beekeeping of the present invention, it acts as a nutrient source to bees, thereby improving the bee's activity and honey-gathering power.

In the beekeeping adjuvant composition of the present invention, the essential oil contains winter green oil, mir oil, spearmint oil and lemon glass oil in a mixing ratio of 1-5: 1-5: 0-2.5: 0-2.5, and spearmint The sum of the oil and lemon glass oil content is characterized in that it does not exceed 50% of the total content of the essential oil, and more preferably does not exceed 30%. Among the essential oil components, if the content of spearmint oil and lemon glass oil exceeds 50% of the total content of the essential oil, it is not preferable because it can kill bees.

In the adjuvant composition for beekeeping of the present invention, the methyl sulfonyl methane, essential oil and carnitine may be included in 0.001 to 5% by weight, preferably 0.05 to 3% by weight, based on the total weight of the adjuvant composition for beekeeping. Preferably, it is characterized in that it contains 0.04 to 0.4% by weight. When the methyl sulfonyl methane, essential oil, and carnitine are contained in an amount of less than 0.001, it is difficult to derive the effect to be realized in the present invention, and when it exceeds 5% by weight, the domination (the bee fight occurs due to a thief bee and the bees die). Occurs, can cause diarrhea in bees, and can negatively affect honey bee production due to its strong scent.

In the adjuvant composition for beekeeping of the present invention, the methyl sulfonyl methane, essential oil and carnitine may be included in 0.002 to 0.2% by weight, 0.006 to 0.6% by weight, and 0.002 to 0.2% by weight, respectively, based on the total weight of the beekeeping adjuvant composition. , Preferably 0.01 to 0.05% by weight, 0.02 to 0.3% by weight, 0.01 to 0.05% by weight may be included. If the methyl sulfonyl methane is less than 0.002% by weight, it is difficult to exhibit the effect of preventing diseases caused by larvae, and if it is more than 0.2% by weight, the improvement of the effect compared to the input of the raw material is insignificant, which is not economically preferable. If the essential oil is less than 0.006% by weight, the effect of enhancing the immunity of bees may be insignificant, and if it exceeds 0.6% by weight, the improvement of the effect compared to the input of the raw material is not economically desirable. If the carnitine is less than 0.002% by weight, it is difficult to supplement the nutrition of bees, and if it exceeds 0.2% by weight, the improvement of the effect compared to the input of the raw material is insignificant, which is not economically preferable.

In the adjuvant composition for beekeeping of the present invention, the adjuvant composition for beekeeping is characterized in that it further comprises at least one component selected from the group consisting of vitamins, proteolytic enzymes, antifungal agents, sugars, taurine and minerals.

In the adjuvant composition for beekeeping of the present invention, the adjuvant composition for beekeeping is characterized in that it further comprises a food additive, an acidity control agent, a formulation control agent, and purified water.

In the adjuvant composition for beekeeping of the present invention, the adjuvant composition for beekeeping is characterized in that it is capable of improving the activity and collecting power of bees and increasing the survival rate.

According to an experimental example of the present invention, a composition containing methyl sulfonyl methane, essential oil and carnitine (Example 1) and a composition not containing the above components (Comparative Example 1) and bees in the untreated group (control group) As a result of confirming the activity, collection power and viability of the bees, when the composition of Example 1 was treated, the activity, honey collection power, and survival rate of bees were increased compared to the control and Comparative Example 1. In other words, it was confirmed that carnitine acts as an energy booster for honey bees, thereby increasing bee activity and collecting power, and methyl sulfonyl methane and essential oil inhibiting bee death due to cold damage by preventing bee diseases caused by larvae. . These results show that the composition according to the present invention can enhance the immunity of bees, thereby inhibiting the death of bees due to disease, and increasing the vitality to increase activity and honey-gathering power (see Experimental Examples 1 to 4).

The adjuvant composition for beekeeping according to the present invention may be prepared in one or more formulations selected from the group consisting of a solution, a suspension, a spray, or a gel.

As described above, the adjuvant composition for beekeeping according to the present invention enhances the immunity of the bees, thereby increasing the bee's activity and honey-gathering power as well as increasing the survival rate of the bees, thereby improving the productivity of beekeeping products. I can.

1 is a diagram showing a result of testing bee activity according to Experimental Example 1. FIG.
2 is a view showing the results of testing the honey bee collecting power according to Experimental Example 2.
3 is a diagram showing the results of testing bee viability according to Experimental Example 3.
4 to 6 are results of confirming the activity (FIG. 4), collecting power (FIG. 5), and viability (FIG. 6) of bees through a filter test according to Experimental Example 4. FIG.
7 is a diagram showing a result of a palatability test according to Experimental Example 5.

Hereinafter, the present invention will be described in more detail through examples. Since these examples are for illustrative purposes only, the scope of the present invention is not to be construed as being limited by these examples.

Example: Preparation of a beekeeping adjuvant composition

After weighing and mixing the raw materials in Table 1, the mixture was stirred at 500 rpm for 2 hours, and filtered through a 75-100 mesh filter to prepare an immunity enhancing agent composition for beekeeping. Essential oils in Table 1 were prepared by mixing winter green oil, mir oil, spearmint oil, and lemon glass oil in a ratio of 1:1:0.5:0.5.

ingredient content(%) Methyl sulfonyl methane 0.03 Essential oil 0.06 L-carnitine 0.03 Mixed vitamins 0.075 Protease NP 0.03 Sodium propionate 0.075 L-histidine 0.03 L-arginine 0.03 glucose 7.5 Taurine 0.075 Zinc gluconate 0.03 Spices 0.04 Enzyme-treated stevia 0.035 Sodium L-glutamate 0.3 Citric acid 0.75 Purified water 89.335 Excipient 1.575 100

Comparative Example 1: Preparation of immunity enhancing agent composition for beekeeping

After weighing and mixing the raw materials in Table 2 below, the mixture was stirred at 500 rpm for 2 hours, and filtered through a 75-100 mesh filter to prepare an immunity enhancing agent composition for beekeeping.

ingredient content(%) Mixed vitamins 0.075 Protease NP 0.03 Sodium propionate 0.075 L-histidine 0.03 L-arginine 0.03 glucose 7.5 Taurine 0.075 Zinc gluconate 0.03 Spices 0.04 Enzyme-treated stevia 0.035 Sodium L-glutamate 0.3 Citric acid 0.75 Purified water 89.455 Excipient 1.575 100

Experimental Example 1: Bee activity test

After feeding the composition prepared in Example and Comparative Example 1 to bees, the number of bees ^{per 10 cm 2} area was measured (camcorder photographing (sony, HDR-CX380)) during the bee life cycle (21 days) to confirm the bee's activity. The results are shown in Table 3 and Fig. 1.

Test start date 7 days later 14 days later 21 days later Control (no treatment) 10 20 33 65 Comparative Example 1 10 22 37 75 Example 10 33 47 90

As a result, as can be seen in Table 3 and FIG. 1, the group treated with the composition prepared in Example increased the activity by 38.5% compared to the control group and 15.4% compared to Comparative Example 1.

Experimental Example 2: Bee collecting power test

After feeding the composition prepared in Example and Comparative Example 1 to bees, the amount of honey per bee group (kg) was measured at the 30th, 45th and 60th day of feeding to confirm the collecting power of the bees. The results are shown in Table 4 and FIG. 2.

Test start date 30 days later 45 days later 60 days later Control (no treatment) 0 2 5 6 Comparative Example 1 0 3 6 7 Example 0 4 7 9

As a result, as can be seen in Table 4 and FIG. 2, the group treated with the composition prepared in Example increased the collection power by 50% compared to the control group and 16.6% compared to Comparative Example 1.

Experimental Example 3: Bee viability test

After feeding the composition prepared in Examples and Comparative Example 1 to ^{bees, the number of bees per 10 cm 2} area was measured during the wintering period from November to January (3 months) to confirm the viability of the bees (overcoming the winter cold sea). . The results are shown in Table 5 and FIG. 3.

Test start date 30 days later 60 days later 90 days later Control (no treatment) 100 85 64 48 Comparative Example 1 100 88 72 55 Example 100 91 85 67

As a result, as can be seen in Table 5 and FIG. 3, the group treated with the composition prepared in Example increased the survival rate by 39.6% compared to the control group and 14.6% compared to Comparative Example 1.

Experimental Example 4: Field Test

At an apiary located in Pyeongtaek-si, Gyeonggi-do, the activity, collecting power, and survival rate of bees were confirmed through field tests through camcorder shooting. Specifically, the activity and collecting power of bees were measured through the dense power of bees in the group fed the composition prepared in the examples to bees (Example) and the group fed the commonly used feeding liquid (control group). It was confirmed, and the survival rate of the bees was confirmed through the degree of death of the bees. The results are shown in FIGS. 4 to 6.

As a result, as can be seen in Figures 4 and 5, it can be confirmed that the density of bees in the group fed the composition prepared in the Example to bees is higher than that of the control group, which is excellent in the activity and collecting power of the bees. Represents. In addition, as can be seen in Figure 6, it can be confirmed that the number of bees killed in the group fed the composition prepared in the example to bees is less than that of the control group, which indicates that the survival rate of the bees is better.

Experimental Example 5: palatability test

The taste of bees was tested through camcorder shooting at a bee farm located in Pyeongtaek, Gyeonggi-do. Specifically, the density of bees in the group fed the composition prepared in the examples to bees (Example) and the group fed the currently marketed seaweed nutrient (Comparative Example 2) and microbial agent (Comparative Example 3) Through this, the palatability of bees was confirmed. The results are shown in FIG. 7.

As a result, as can be seen in FIG. 7, it is seen that bees are concentrated in the tray on which the composition prepared in the Example is supported, indicating that the palatability is superior to that of the comparative examples.

Claims (8)

In the beekeeping adjuvant composition comprising methyl sulfonyl methane, essential oil and carnitine as active ingredients,
The essential oil includes winter green oil, myr oil, spearmint oil, and lemon glass oil in a mixing ratio of 1-5: 1-5: 0-2.5: 0-2.5, and the sum of spearmint oil and lemon glass oil is essential. That does not exceed 50% of the total oil content, beekeeping adjuvant composition.
delete delete The method of claim 1,
The methyl sulfonyl methane, essential oil, and carnitine are contained in an adjuvant composition for beekeeping in an amount of 0.001 to 5% by weight based on the total weight of the adjuvant composition for beekeeping.
The method of claim 1,
The methyl sulfonyl methane, essential oil, and carnitine are each contained in 0.002 to 0.2% by weight, 0.006 to 0.6% by weight, and 0.002 to 0.2% by weight based on the total weight of the beekeeping adjuvant composition, respectively.
The method of claim 1,
The beekeeping composition further comprises one or more components selected from the group consisting of vitamins, proteolytic enzymes, antifungal agents, sugars, taurine, and minerals.
The method of claim 1,
The beekeeping adjuvant composition further comprises a food additive, an acidity modifier, a formulation modifier, and purified water.
The method of claim 1,
The adjuvant composition for beekeeping improves the activity and gathering power of bees, and can increase the survival rate, the adjuvant composition for beekeeping.

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