RU2021101009A - Кэпированные и некэпированные цистеины антитела, и их применение в конъюгации антитело - лекарственное средство - Google Patents
Кэпированные и некэпированные цистеины антитела, и их применение в конъюгации антитело - лекарственное средство Download PDFInfo
- Publication number
- RU2021101009A RU2021101009A RU2021101009A RU2021101009A RU2021101009A RU 2021101009 A RU2021101009 A RU 2021101009A RU 2021101009 A RU2021101009 A RU 2021101009A RU 2021101009 A RU2021101009 A RU 2021101009A RU 2021101009 A RU2021101009 A RU 2021101009A
- Authority
- RU
- Russia
- Prior art keywords
- antibody
- fragments
- predetermined capping
- capped
- group
- Prior art date
Links
- 208000016113 North Carolina macular dystrophy Diseases 0.000 title 1
- 229940125644 antibody drug Drugs 0.000 title 1
- 230000021615 conjugation Effects 0.000 title 1
- 239000012634 fragment Substances 0.000 claims 4
- 238000000034 method Methods 0.000 claims 3
- 238000004113 cell culture Methods 0.000 claims 2
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 claims 2
- 231100000433 cytotoxic Toxicity 0.000 claims 2
- 230000001472 cytotoxic effect Effects 0.000 claims 2
- 229940127121 immunoconjugate Drugs 0.000 claims 2
- 229910052717 sulfur Inorganic materials 0.000 claims 2
- 239000011593 sulfur Substances 0.000 claims 2
- 239000004698 Polyethylene Substances 0.000 claims 1
- 150000001299 aldehydes Chemical group 0.000 claims 1
- 125000003342 alkenyl group Chemical group 0.000 claims 1
- 125000000217 alkyl group Chemical group 0.000 claims 1
- 125000000304 alkynyl group Chemical group 0.000 claims 1
- 125000003118 aryl group Chemical group 0.000 claims 1
- 229930195731 calicheamicin Natural products 0.000 claims 1
- VVFZXPZWVJMYPX-UHFFFAOYSA-N dbco-peg4--maleimide Chemical compound C1C2=CC=CC=C2C#CC2=CC=CC=C2N1C(=O)CCNC(=O)CCOCCOCCOCCOCCNC(=O)CCN1C(=O)C=CC1=O VVFZXPZWVJMYPX-UHFFFAOYSA-N 0.000 claims 1
- 125000001072 heteroaryl group Chemical group 0.000 claims 1
- 150000002576 ketones Chemical group 0.000 claims 1
- OGSSEPXEBBNVGN-UHFFFAOYSA-N n-[2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethyl]-3-(2,5-dioxopyrrol-1-yl)propanamide Chemical compound [N-]=[N+]=NCCOCCOCCOCCNC(=O)CCN1C(=O)C=CC1=O OGSSEPXEBBNVGN-UHFFFAOYSA-N 0.000 claims 1
- 229920000573 polyethylene Polymers 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/05—Dipeptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
- A61K47/6801—Drug-antibody or immunoglobulin conjugates defined by the pharmacologically or therapeutically active agent
- A61K47/6803—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates
- A61K47/6811—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates the drug being a protein or peptide, e.g. transferrin or bleomycin
- A61K47/6817—Toxins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
- A61K47/6835—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
- A61K47/6835—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site
- A61K47/6851—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site the antibody targeting a determinant of a tumour cell
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
- A61K47/6835—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site
- A61K47/6851—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site the antibody targeting a determinant of a tumour cell
- A61K47/6855—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site the antibody targeting a determinant of a tumour cell the tumour determinant being from breast cancer cell
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
- A61K47/6889—Conjugates wherein the antibody being the modifying agent and wherein the linker, binder or spacer confers particular properties to the conjugates, e.g. peptidic enzyme-labile linkers or acid-labile linkers, providing for an acid-labile immuno conjugate wherein the drug may be released from its antibody conjugated part in an acidic, e.g. tumoural or environment
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/52—Constant or Fc region; Isotype
- C07K2317/526—CH3 domain
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
- C07K2317/94—Stability, e.g. half-life, pH, temperature or enzyme-resistance
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Oncology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicinal Preparation (AREA)
Claims (9)
1. Способ получения конъюгата лекарственного средства с антителом (ADC), включающий стадии:
(a) получения кэпированного антитела в клеточной культуре, причем один или несколько непарных остатков цистеина на указанном антителе являются ковалентно связанными посредством связей серы с одним или несколькими предварительно определенными фрагментами кэпирования, где указанные предварительно определенные фрагменты кэпирования выбирают из группы, состоящей из:
где R представляет собой алкил, алкенил, алкинил, арил, гетероарил, альдегид, кетон или цитотоксическую нагрузку; и
(b) необязательно дополнительно взаимодействие указанного предварительно определенного фрагмента кэпирования на указанном кэпированном антителе.
2. Способ по п. 2, где указанную цитотоксическую нагрузку выбирают из группы, состоящей из ауристатинов, калихеамицинов, майтанзиноидов, сплайсостатинов, димеров CBI, димеров CPI и димеров CTI.
3. Способ получения конъюгата лекарственного средства с антителом (ADC), включающий стадии:
(a) получения кэпированного антитела в клеточной культуре, причем один или несколько непарных остатков цистеина на указанном антителе являются ковалентно связанными посредством связей серы с одним или несколькими предварительно определенными фрагментами кэпирования, где указанные предварительно определенные фрагменты кэпирования выбирают из группы, состоящей из: малеимидотриокса-4-формилбензамида (MTFB), дибензоциклооктил-полиэтиленмалеимада (DBCO-PEG4-малеимида) и азидо-ПЭГ3-малеимида; и
(b) необязательно дополнительно взаимодействие указанного предварительно определенного фрагмента кэпирования на указанном кэпированном антителе.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201562204005P | 2015-08-12 | 2015-08-12 | |
US62/204,005 | 2015-08-12 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
RU2018105128A Division RU2742260C2 (ru) | 2015-08-12 | 2016-08-09 | Кэпированные и некэпированные цистеины антитела и их применение в конъюгации антитело-лекарственное средство |
Publications (1)
Publication Number | Publication Date |
---|---|
RU2021101009A true RU2021101009A (ru) | 2021-02-20 |
Family
ID=56855760
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
RU2018105128A RU2742260C2 (ru) | 2015-08-12 | 2016-08-09 | Кэпированные и некэпированные цистеины антитела и их применение в конъюгации антитело-лекарственное средство |
RU2021101009A RU2021101009A (ru) | 2015-08-12 | 2016-08-09 | Кэпированные и некэпированные цистеины антитела, и их применение в конъюгации антитело - лекарственное средство |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
RU2018105128A RU2742260C2 (ru) | 2015-08-12 | 2016-08-09 | Кэпированные и некэпированные цистеины антитела и их применение в конъюгации антитело-лекарственное средство |
Country Status (20)
Country | Link |
---|---|
US (2) | US10653794B2 (ru) |
EP (2) | EP3334760B1 (ru) |
JP (2) | JP6865209B2 (ru) |
KR (2) | KR102319622B1 (ru) |
CN (1) | CN107849121B (ru) |
AU (1) | AU2016305331B2 (ru) |
BR (1) | BR112018001904A2 (ru) |
CA (1) | CA2938333A1 (ru) |
DK (1) | DK3334760T3 (ru) |
ES (1) | ES2864842T3 (ru) |
HK (1) | HK1252799A1 (ru) |
HR (1) | HRP20210579T1 (ru) |
HU (1) | HUE053956T2 (ru) |
IL (1) | IL257420B (ru) |
MX (1) | MX2018001686A (ru) |
PL (1) | PL3334760T3 (ru) |
PT (1) | PT3334760T (ru) |
RU (2) | RU2742260C2 (ru) |
SI (1) | SI3334760T1 (ru) |
WO (1) | WO2017025897A2 (ru) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BR112018001904A2 (pt) * | 2015-08-12 | 2018-09-18 | Pfizer | cisteínas de anticorpos rematados e não rematados, e seu uso na conjugação anticorpo-fármaco |
US20190381182A1 (en) | 2017-02-08 | 2019-12-19 | Pfizer Inc. | Large scale production process for capped and un-capped antibody cysteines and their use in therapeutic protein conjugation |
WO2020164561A1 (en) | 2019-02-15 | 2020-08-20 | Wuxi Biologics (Shanghai) Co. Ltd. | Process for preparing antibody-drug conjugates with improved homogeneity |
IL303951A (en) * | 2020-12-22 | 2023-08-01 | Novartis Ag | Methods for reducing the oxidation level of cysteine residues in recombinantly secreted protein during cell culture |
WO2023067626A1 (en) * | 2021-10-20 | 2023-04-27 | Dr. Reddy's Laboratories Limited | A cell culture process to produce an antibody composition |
Family Cites Families (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5241078A (en) * | 1988-06-14 | 1993-08-31 | Cetus Oncology | Coupling agents and sterically hindered disulfide linked conjugates prepared therefrom |
WO1993006217A1 (en) * | 1991-09-19 | 1993-04-01 | Genentech, Inc. | EXPRESSION IN E. COLI OF ANTIBODY FRAGMENTS HAVING AT LEAST A CYSTEINE PRESENT AS A FREE THIOL, USE FOR THE PRODUCTION OF BIFUNCTIONAL F(ab')2 ANTIBODIES |
ATE526401T1 (de) * | 1999-01-14 | 2011-10-15 | Bolder Biotechnology Inc | Verfahren zur herstellung von proteinen mit freien cysteinresten |
JP4249028B2 (ja) * | 2001-12-04 | 2009-04-02 | 田辺三菱製薬株式会社 | 蛋白質の活性化方法 |
JP5335422B2 (ja) * | 2005-06-17 | 2013-11-06 | ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト | 少なくとも1つの非天然のシステインを含んでいる操作されたタンパク質の選択的な還元および誘導体化 |
RU2424246C2 (ru) * | 2006-05-26 | 2011-07-20 | Ипсен Фарма С.А.С. | Способы сайт-специфического пегилирования |
US8612161B2 (en) * | 2008-03-19 | 2013-12-17 | Intelligent Biosystems Inc. | Methods and compositions for base calling nucleic acids |
PL2586788T3 (pl) * | 2007-07-09 | 2018-05-30 | Genentech, Inc. | Zapobieganie redukcji wiązań disiarczkowych podczas rekombinacyjnego wytwarzania polipeptydów |
WO2009092011A1 (en) * | 2008-01-18 | 2009-07-23 | Medimmune, Llc | Cysteine engineered antibodies for site-specific conjugation |
KR101962476B1 (ko) | 2009-07-03 | 2019-03-26 | 아비펩 피티와이 리미티트 | 면역성 접합체 및 그 제조방법 |
WO2011041721A1 (en) * | 2009-10-02 | 2011-04-07 | Biogen Idec Ma Inc. | Methods of preventing and removing trisulfide bonds |
EP3778917A3 (en) * | 2009-12-04 | 2021-06-09 | F. Hoffmann-La Roche AG | Multispecific antibodies, antibody analogs, compositions, and methods |
MX336540B (es) * | 2010-06-08 | 2016-01-22 | Genentech Inc | Conjugados y anticuerpos manipulados geneticamente con cisteina. |
MX339533B (es) * | 2011-05-27 | 2016-05-30 | Abbvie Biotechnology Ltd | Composiciones y metodos de daclizumab (dac) del proceso de alto rendimiento (hyp). |
EP2818480B1 (en) * | 2012-02-24 | 2020-08-26 | Alteogen Inc. | Modified antibody in which motif comprising cysteine residue is bound, modified antibody-drug conjugate comprising the modified antibody, and production method for same |
US9018172B2 (en) | 2012-09-26 | 2015-04-28 | Massachusetts Institute Of Technology | Modification of peptides via SNAr reactions of thiols with fluorinated aromatics |
AR098221A1 (es) * | 2013-11-04 | 2016-05-18 | Pfizer | Conjugados de anticuerpo anti-efna4-fármaco |
EP3077522A1 (en) * | 2013-12-04 | 2016-10-12 | Immunogen, Inc. | Compositions and methods for antibody production |
AU2015208814B2 (en) | 2014-01-27 | 2018-11-01 | Pfizer Inc. | Bifunctional cytotoxic agents |
SG11201706991YA (en) | 2015-03-20 | 2017-10-30 | Pfizer | Bifunctional cytotoxic agents containing the cti pharmacophore |
BR112018001904A2 (pt) * | 2015-08-12 | 2018-09-18 | Pfizer | cisteínas de anticorpos rematados e não rematados, e seu uso na conjugação anticorpo-fármaco |
-
2016
- 2016-08-09 BR BR112018001904A patent/BR112018001904A2/pt active Search and Examination
- 2016-08-09 PL PL16760548T patent/PL3334760T3/pl unknown
- 2016-08-09 CN CN201680047377.XA patent/CN107849121B/zh active Active
- 2016-08-09 RU RU2018105128A patent/RU2742260C2/ru active
- 2016-08-09 RU RU2021101009A patent/RU2021101009A/ru unknown
- 2016-08-09 EP EP16760548.4A patent/EP3334760B1/en active Active
- 2016-08-09 ES ES16760548T patent/ES2864842T3/es active Active
- 2016-08-09 PT PT167605484T patent/PT3334760T/pt unknown
- 2016-08-09 HU HUE16760548A patent/HUE053956T2/hu unknown
- 2016-08-09 EP EP20207006.6A patent/EP3808768A1/en active Pending
- 2016-08-09 CA CA2938333A patent/CA2938333A1/en active Pending
- 2016-08-09 JP JP2018506438A patent/JP6865209B2/ja active Active
- 2016-08-09 DK DK16760548.4T patent/DK3334760T3/da active
- 2016-08-09 KR KR1020207023230A patent/KR102319622B1/ko active IP Right Grant
- 2016-08-09 US US15/748,268 patent/US10653794B2/en active Active
- 2016-08-09 AU AU2016305331A patent/AU2016305331B2/en active Active
- 2016-08-09 KR KR1020187006927A patent/KR102146617B1/ko active IP Right Grant
- 2016-08-09 WO PCT/IB2016/054789 patent/WO2017025897A2/en active Application Filing
- 2016-08-09 SI SI201631163T patent/SI3334760T1/sl unknown
- 2016-08-09 MX MX2018001686A patent/MX2018001686A/es unknown
- 2016-08-09 IL IL257420A patent/IL257420B/en unknown
-
2018
- 2018-09-20 HK HK18112100.2A patent/HK1252799A1/zh unknown
-
2020
- 2020-03-03 US US16/807,375 patent/US11980669B2/en active Active
-
2021
- 2021-04-05 JP JP2021063975A patent/JP2021118694A/ja active Pending
- 2021-04-12 HR HRP20210579TT patent/HRP20210579T1/hr unknown
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
RU2021101009A (ru) | Кэпированные и некэпированные цистеины антитела, и их применение в конъюгации антитело - лекарственное средство | |
WO2018098269A3 (en) | Peptide-containing linkers for antibody-drug conjugates | |
RU2019109456A (ru) | Новые конъюгаты антител и их применения | |
BR112017019300A2 (pt) | conjugado de fórmula genérica ama - l - x - s - ab, método para sintetizar um conjugado de fórmula genérica ama - l - x - s - ab, kit, método para sintetizar o composto ama - l - x', composição farmacêutica e método para tratar uma doença associada a células que apresentam um alvo | |
HRP20210170T1 (hr) | Protutijela protiv b7-h3 i konjugati protutijela s lijekom | |
PH12016500658B1 (en) | Human anti-alpha-synuclein autoantibodies | |
CY1122210T1 (el) | Ανθρωπινα αντισωματα anti-sod1 | |
BR112016002829A2 (pt) | Composto e processo para preparar o composto de conjugado anticorpo-¿droga, composição farmacêutica, método de tratamento do câncer, kit para o tratamento do câncer, intermediário ligante¿-droga, porção e composto de porção droga de dímero cbi | |
EP3792278A3 (en) | Human anti-tau antibodies | |
BR112018013407A2 (pt) | anticorpos e conjugados dos mesmos | |
CR10273A (es) | "anticuerpos anti-5t4 y usos de los mismos" | |
NO20085396L (no) | Antistoffer og immunkonjugater, og anvendelser derav | |
DK3276349T3 (da) | Haptener, haptenkonjugater, sammensætninger deraf og fremgangsmåder til fremstilling og anvendelse deraf | |
EA201690744A1 (ru) | Конъюгаты белок-полимер-лекарственное средство | |
WO2017004026A8 (en) | Anti-cd123 antibodies and conjugates and derivatives thereof | |
DK1594542T3 (da) | Anti-CD70 antistof-lægemiddelkonjugater og deres anvendelse ved behandling af cancer | |
WO2015151081A3 (en) | Bridge linkers for conjugation of cell-binding molecules | |
WO2006113909A3 (en) | Humanized anti-cd70 binding agents and uses thereof | |
MX350200B (es) | Anticuerpos humanos y conjugados de anticuerpo-farmaco contra cd74. | |
WO2019215510A8 (en) | Compositions and methods related to anti-cd19 antibody drug conjugates | |
NL1033650A1 (nl) | Antilichamen tegen myostatine. | |
PE20150892A1 (es) | Anticuerpos anti-notch y conjugados de anticuerpo-farmaco | |
DE602005025531D1 (de) | Saccharid-konjugatvakzine | |
AR102649A1 (es) | Anticuerpos anti-cadherina-p y conjugados anticuerpos-fármacos | |
EP3695852A4 (en) | ANTI-MESOTHELIN ANTIBODY AND ANTIBODY-ASSOCIATED DRUG CONJUGATE |