RU2014144504A - Вырабатывающие рна микроорганизмы, полученные способом генной инженерии - Google Patents
Вырабатывающие рна микроорганизмы, полученные способом генной инженерии Download PDFInfo
- Publication number
- RU2014144504A RU2014144504A RU2014144504A RU2014144504A RU2014144504A RU 2014144504 A RU2014144504 A RU 2014144504A RU 2014144504 A RU2014144504 A RU 2014144504A RU 2014144504 A RU2014144504 A RU 2014144504A RU 2014144504 A RU2014144504 A RU 2014144504A
- Authority
- RU
- Russia
- Prior art keywords
- pha
- microorganism
- genetically modified
- specified
- synthase
- Prior art date
Links
- 244000005700 microbiome Species 0.000 title claims abstract 35
- 238000000034 method Methods 0.000 title claims 5
- 238000010353 genetic engineering Methods 0.000 title 1
- 239000005014 poly(hydroxyalkanoate) Substances 0.000 claims abstract 19
- 108090000623 proteins and genes Proteins 0.000 claims abstract 18
- 229920000903 polyhydroxyalkanoate Polymers 0.000 claims abstract 17
- 238000004519 manufacturing process Methods 0.000 claims abstract 9
- 108010010718 poly(3-hydroxyalkanoic acid) synthase Proteins 0.000 claims abstract 8
- 102000004169 proteins and genes Human genes 0.000 claims abstract 6
- 230000015556 catabolic process Effects 0.000 claims abstract 5
- 238000006731 degradation reaction Methods 0.000 claims abstract 5
- 238000012239 gene modification Methods 0.000 claims abstract 5
- 230000005017 genetic modification Effects 0.000 claims abstract 5
- 235000013617 genetically modified food Nutrition 0.000 claims abstract 5
- 230000002779 inactivation Effects 0.000 claims abstract 4
- 230000004048 modification Effects 0.000 claims abstract 4
- 238000012986 modification Methods 0.000 claims abstract 4
- BYKRNSHANADUFY-UHFFFAOYSA-M sodium octanoate Chemical compound [Na+].CCCCCCCC([O-])=O BYKRNSHANADUFY-UHFFFAOYSA-M 0.000 claims abstract 4
- 238000001727 in vivo Methods 0.000 claims abstract 2
- 230000001105 regulatory effect Effects 0.000 claims abstract 2
- 230000002194 synthesizing effect Effects 0.000 claims abstract 2
- 239000002609 medium Substances 0.000 claims 5
- 241000589776 Pseudomonas putida Species 0.000 claims 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims 2
- 241000589516 Pseudomonas Species 0.000 claims 2
- 239000000411 inducer Substances 0.000 claims 2
- 241000190857 Allochromatium vinosum Species 0.000 claims 1
- 241000193033 Azohydromonas lata Species 0.000 claims 1
- 241000589151 Azotobacter Species 0.000 claims 1
- 241000010804 Caulobacter vibrioides Species 0.000 claims 1
- 241000588879 Chromobacterium violaceum Species 0.000 claims 1
- 241000252867 Cupriavidus metallidurans Species 0.000 claims 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims 1
- 241000589540 Pseudomonas fluorescens Species 0.000 claims 1
- 241000589615 Pseudomonas syringae Species 0.000 claims 1
- 241000316848 Rhodococcus <scale insect> Species 0.000 claims 1
- 239000003242 anti bacterial agent Substances 0.000 claims 1
- 229940088710 antibiotic agent Drugs 0.000 claims 1
- 230000003115 biocidal effect Effects 0.000 claims 1
- 125000004432 carbon atom Chemical group C* 0.000 claims 1
- 238000000605 extraction Methods 0.000 claims 1
- 239000008187 granular material Substances 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 230000003834 intracellular effect Effects 0.000 claims 1
- 238000002955 isolation Methods 0.000 claims 1
- 150000002576 ketones Chemical class 0.000 claims 1
- 101150053431 phaZ gene Proteins 0.000 claims 1
- 108010081808 poly(3-hydroxyalkanoic acid) depolymerase Proteins 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
- C12P7/625—Polyesters of hydroxy carboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/38—Pseudomonas
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/874—Pseudomonas
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12163787 | 2012-04-11 | ||
| EP12163787.0 | 2012-04-11 | ||
| PCT/EP2013/057630 WO2013153180A1 (en) | 2012-04-11 | 2013-04-11 | Pha-producing genetically engineered microorganisms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2014144504A true RU2014144504A (ru) | 2016-06-10 |
Family
ID=48128294
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2014144504A RU2014144504A (ru) | 2012-04-11 | 2013-04-11 | Вырабатывающие рна микроорганизмы, полученные способом генной инженерии |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20150203878A1 (enExample) |
| EP (1) | EP2836601A1 (enExample) |
| JP (1) | JP2015512648A (enExample) |
| KR (1) | KR20150004830A (enExample) |
| CN (1) | CN104520433A (enExample) |
| CA (1) | CA2869891A1 (enExample) |
| HK (1) | HK1206787A1 (enExample) |
| IN (1) | IN2014MN02184A (enExample) |
| RU (1) | RU2014144504A (enExample) |
| SG (1) | SG11201405636QA (enExample) |
| WO (1) | WO2013153180A1 (enExample) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2594637B1 (en) * | 2010-07-14 | 2014-11-12 | Toyota Jidosha Kabushiki Kaisha | Mutant polyhydroxyalkanoic acid synthase gene and method for producing aliphatic polyester using same |
| JP6313708B2 (ja) * | 2012-10-22 | 2018-04-18 | 株式会社カネカ | 高分子量ｐｈａ生産微生物とそれを用いた高分子量ｐｈａの製造方法 |
| CN104328062A (zh) * | 2014-06-13 | 2015-02-04 | 东北师范大学 | 一株假单胞菌诱变菌株及其在生产(r)-3-羟基丁酸中的应用 |
| EP3551586A4 (en) * | 2016-12-09 | 2020-07-29 | Knipbio, Inc. | MICROBIAL PRODUCTION OF PROTEIN AND PHB BY BACTERIA USING ALCOHOL |
| CN110004182B (zh) * | 2019-04-02 | 2021-03-05 | 清华大学 | 一种微生物胞内大颗粒内含物的制备方法及其应用 |
| CN111909952B (zh) * | 2020-08-04 | 2024-04-26 | 北京大学深圳研究院 | 构建食烷菌敲除酰基辅酶a硫脂酶基因的方法和应用 |
| CN111982875A (zh) * | 2020-08-20 | 2020-11-24 | 北京大学深圳研究院 | 一种基于三维荧光光谱分析的产聚羟基脂肪酸酯菌的筛选方法 |
| EP4347698A4 (en) * | 2021-05-28 | 2025-06-11 | Kimberly-Clark Worldwide, Inc. | Methods and systems for single-step decontamination and enzymatic degradation of bio-based polymers |
| CN116121219B (zh) * | 2022-04-06 | 2023-11-28 | 深圳蓝晶生物科技有限公司 | 表达乙酰乙酰辅酶a还原酶变体的工程化微生物及提高pha产量的方法 |
| CN116445440B (zh) * | 2022-10-26 | 2024-01-02 | 华南理工大学 | 黏性聚羟基脂肪酸酯及其制备和应用 |
| WO2024197106A2 (en) * | 2023-03-21 | 2024-09-26 | The Regents Of The University Of California | Continuous polyhydroxyalkanoate production by perchlorate respiring microorganisms |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4417169A1 (de) * | 1994-05-17 | 1995-11-23 | Vogelbusch Gmbh | Rekombinante Herstellung von Polyhydroxyalkanoaten in glucosefreiem Medium |
| ATE323152T1 (de) * | 1997-09-19 | 2006-04-15 | Metabolix Inc | Biologische systeme zur herstellung von polyhydroxyalkanoate polymeren die 4-hydroxysäure enthalten |
-
2013
- 2013-04-11 EP EP13716773.0A patent/EP2836601A1/en not_active Withdrawn
- 2013-04-11 SG SG11201405636QA patent/SG11201405636QA/en unknown
- 2013-04-11 IN IN2184MUN2014 patent/IN2014MN02184A/en unknown
- 2013-04-11 JP JP2015504961A patent/JP2015512648A/ja active Pending
- 2013-04-11 KR KR1020147031299A patent/KR20150004830A/ko not_active Withdrawn
- 2013-04-11 WO PCT/EP2013/057630 patent/WO2013153180A1/en not_active Ceased
- 2013-04-11 CA CA2869891A patent/CA2869891A1/en not_active Abandoned
- 2013-04-11 RU RU2014144504A patent/RU2014144504A/ru not_active Application Discontinuation
- 2013-04-11 CN CN201380019207.7A patent/CN104520433A/zh active Pending
- 2013-04-11 US US14/391,939 patent/US20150203878A1/en not_active Abandoned
- 2013-04-11 HK HK15107210.2A patent/HK1206787A1/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US20150203878A1 (en) | 2015-07-23 |
| SG11201405636QA (en) | 2014-11-27 |
| JP2015512648A (ja) | 2015-04-30 |
| IN2014MN02184A (enExample) | 2015-08-28 |
| CA2869891A1 (en) | 2013-10-17 |
| HK1206787A1 (en) | 2016-01-15 |
| WO2013153180A1 (en) | 2013-10-17 |
| CN104520433A (zh) | 2015-04-15 |
| EP2836601A1 (en) | 2015-02-18 |
| KR20150004830A (ko) | 2015-01-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2014144504A (ru) | Вырабатывающие рна микроорганизмы, полученные способом генной инженерии | |
| Han et al. | Molecular characterization of the phaEC Hm genes, required for biosynthesis of poly (3-hydroxybutyrate) in the extremely halophilic archaeon Haloarcula marismortui | |
| Chien et al. | Production of poly-β-hydroxybutyrate (PHB) by Vibrio spp. isolated from marine environment | |
| Sabirova et al. | Mutation in a “tesB-like” hydroxyacyl-coenzyme A-specific thioesterase gene causes hyperproduction of extracellular polyhydroxyalkanoates by Alcanivorax borkumensis SK2 | |
| EP1913135B9 (en) | Extracellular polyhydroxyalkanoates produced by genetically engineered microorganisms | |
| Zhao et al. | Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) production by Haloarchaeon Halogranum amylolyticum | |
| CN108291231B (zh) | 由3-羟基丙酸诱导表达的启动子系统及用其生物生产3-羟基丙酸的方法 | |
| Mozejko-Ciesielska et al. | Transcriptome remodeling of Pseudomonas putida KT2440 during mcl-PHAs synthesis: effect of different carbon sources and response to nitrogen stress | |
| Christensen et al. | High natural PHA production from acetate in Cobetia sp. MC34 and Cobetia marina DSM 4741T and in silico analyses of the genus specific PhaC2 polymerase variant | |
| Mozejko-Ciesielska et al. | Medium-chain-length polyhydroxyalkanoates synthesis by Pseudomonas putida KT2440 relA/spoT mutant: bioprocess characterization and transcriptome analysis | |
| WO2016021604A1 (ja) | 糖質原料からの共重合ポリヒドロキシアルカン酸の製造法 | |
| Ciesielski et al. | The influence of nitrogen limitation on mcl-PHA synthesis by two newly isolated strains of Pseudomonas sp. | |
| Hang et al. | PCR cloning of polyhydroxyalkanoate biosynthesis genes from Burkholderia caryophylli and their functional expression in recombinant Escherichia coli | |
| Grzesiak et al. | Genetic engineering of low-temperature polyhydroxyalkanoate production by Acidovorax sp. A1169, a psychrophile isolated from a subglacial outflow | |
| Ryu et al. | Isoeugenol monooxygenase and its putative regulatory gene are located in the eugenol metabolic gene cluster in Pseudomonas nitroreducens Jin1 | |
| US20160040197A1 (en) | PRODUCTION METHOD FOR COPOLYMER POLYHYDROXYALKANOATE USING GENETICALLY MODIFIED STRAIN OF FATTY ACID ß-OXIDATION PATHWAY | |
| WO2015133468A1 (ja) | ポリエステル顆粒結合タンパク質遺伝子座を改変した組換え株による共重合ポリエステルの製造法 | |
| Marín et al. | Modified 3-oxoadipate pathway for the biodegradation of methylaromatics in Pseudomonas reinekei MT1 | |
| Aziz | Investigating microbial quorum sensing potential for enhanced production of biodegradable polymers | |
| JP5103619B2 (ja) | 共重合ポリエステルの製造法 | |
| Yang et al. | High-temperature stimulation enhances polyhydroxyalkanoates accumulation in thermophile Aeribacillus pallidus BK1 | |
| TWI404799B (zh) | 極端高鹽古菌之聚羥基烷酯生合成基因ｐｈａＲ及其基因產物於生物塑膠生產的應用 | |
| US7135316B2 (en) | Escherichia coli having accession No. PTA 1579 and its use to produce polyhydroxybutyrate | |
| Jiang | Polyhydroxyalkanoates production by bacterial enrichments | |
| TWI386486B (zh) | 極端高鹽古菌之聚羥基烷酯生合成基因群組及酵素於生物塑膠生產的應用 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA93 | Acknowledgement of application withdrawn (no request for examination) |
Effective date: 20160412 |