RU2012133474A - Очистка факторов свертывания крови - Google Patents
Очистка факторов свертывания крови Download PDFInfo
- Publication number
- RU2012133474A RU2012133474A RU2012133474/10A RU2012133474A RU2012133474A RU 2012133474 A RU2012133474 A RU 2012133474A RU 2012133474/10 A RU2012133474/10 A RU 2012133474/10A RU 2012133474 A RU2012133474 A RU 2012133474A RU 2012133474 A RU2012133474 A RU 2012133474A
- Authority
- RU
- Russia
- Prior art keywords
- gla
- protein
- polypeptide
- gamma
- carboxyglutamic acid
- Prior art date
Links
- 239000008280 blood Substances 0.000 title 1
- 210000004369 blood Anatomy 0.000 title 1
- 238000004140 cleaning Methods 0.000 title 1
- 238000000034 method Methods 0.000 claims abstract 27
- UHBYWPGGCSDKFX-VKHMYHEASA-N gamma-carboxy-L-glutamic acid Chemical compound OC(=O)[C@@H](N)CC(C(O)=O)C(O)=O UHBYWPGGCSDKFX-VKHMYHEASA-N 0.000 claims abstract 14
- 229920001184 polypeptide Polymers 0.000 claims abstract 14
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract 14
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract 14
- 102000004169 proteins and genes Human genes 0.000 claims abstract 13
- 108090000623 proteins and genes Proteins 0.000 claims abstract 13
- 239000000463 material Substances 0.000 claims abstract 9
- 102100022641 Coagulation factor IX Human genes 0.000 claims abstract 8
- 108010076282 Factor IX Proteins 0.000 claims abstract 8
- 229960004222 factor ix Drugs 0.000 claims abstract 8
- 239000000203 mixture Substances 0.000 claims abstract 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims 4
- 239000001110 calcium chloride Substances 0.000 claims 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims 4
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims 3
- 229920002684 Sepharose Polymers 0.000 claims 3
- 229910001424 calcium ion Inorganic materials 0.000 claims 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- 239000011324 bead Substances 0.000 claims 2
- 239000012149 elution buffer Substances 0.000 claims 2
- -1 3.0 Chemical compound 0.000 claims 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims 1
- 238000005571 anion exchange chromatography Methods 0.000 claims 1
- 239000000872 buffer Substances 0.000 claims 1
- 239000011575 calcium Substances 0.000 claims 1
- 229910052791 calcium Inorganic materials 0.000 claims 1
- 150000001768 cations Chemical class 0.000 claims 1
- 239000006167 equilibration buffer Substances 0.000 claims 1
- 125000005647 linker group Chemical group 0.000 claims 1
- 239000011777 magnesium Substances 0.000 claims 1
- 229910052749 magnesium Inorganic materials 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 239000011534 wash buffer Substances 0.000 claims 1
- 102000010410 Nogo Proteins Human genes 0.000 abstract 1
- 108010077641 Nogo Proteins Proteins 0.000 abstract 1
- 238000010828 elution Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/647—Blood coagulation factors not provided for in a preceding group or according to more than one of the proceeding groups
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/644—Coagulation factor IXa (3.4.21.22)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21022—Coagulation factor IXa (3.4.21.22)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP10150980 | 2010-01-18 | ||
| EP10150980.0 | 2010-01-18 | ||
| US29701110P | 2010-01-21 | 2010-01-21 | |
| US61/297,011 | 2010-01-21 | ||
| PCT/EP2011/050594 WO2011086197A1 (en) | 2010-01-18 | 2011-01-18 | Purification of blood coagulation factors |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2012133474A true RU2012133474A (ru) | 2014-02-27 |
Family
ID=42173365
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2012133474/10A RU2012133474A (ru) | 2010-01-18 | 2011-01-18 | Очистка факторов свертывания крови |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US9896677B2 (https=) |
| EP (1) | EP2526115B1 (https=) |
| JP (1) | JP6250931B2 (https=) |
| KR (1) | KR20120118028A (https=) |
| CN (1) | CN102939299B (https=) |
| BR (1) | BR112012017696A2 (https=) |
| RU (1) | RU2012133474A (https=) |
| WO (1) | WO2011086197A1 (https=) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112012017696A2 (pt) * | 2010-01-18 | 2017-10-03 | Novo Nordisk Healthcare Ag | Purificação de fatores de coagulação de sangue |
| CN103539852B (zh) * | 2012-07-12 | 2015-08-12 | 上海泰龙生物医药科技有限公司 | 一种从细胞培养液中分离纯化重组人凝血八因子的方法 |
| CN105188749B (zh) | 2012-12-21 | 2017-12-19 | 西雅图基因公司 | 抗ntb‑a抗体及相关组合物和方法 |
| US10643423B2 (en) | 2016-09-23 | 2020-05-05 | Sg Gaming, Inc. | System and digital table for binding a mobile device to a position at the table for transactions |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6258938B1 (en) | 1983-10-28 | 2001-07-10 | Ne Medical Center Hospital, Inc. | Method for the purification and isolation of blood clotting proteins using conformation specific antibodies |
| US4902614A (en) | 1984-12-03 | 1990-02-20 | Teijin Limited | Monoclonal antibody to human protein C |
| FR2632309B1 (fr) | 1988-06-07 | 1990-08-24 | Lille Transfusion Sanguine | Procede de purification par voie chromatographique de proteines, notamment de facteur viii, et les produits obtenus |
| US4981952A (en) | 1988-10-04 | 1991-01-01 | Eli Lilly And Company | Method for the purification of vitamin K-dependent proteins |
| DE69231401T2 (de) | 1991-03-01 | 2001-02-08 | Rhone-Poulenc Rorer International (Holdings) Inc., Greenville | Herstellung von faktor-ix |
| IT1262899B (it) | 1992-03-27 | 1996-07-22 | Sclavo Spa | Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano |
| DE4406515C1 (de) | 1994-02-28 | 1995-10-19 | Immuno Ag | Verfahren zur Isolierung und Reinigung Vitamin K-abhängiger Proteine |
| US6451987B1 (en) | 1999-03-15 | 2002-09-17 | Novo Nordisk A/S | Ion exchange chromatography of proteins and peptides |
| SE0000675D0 (sv) | 2000-03-02 | 2000-03-02 | Protease Ab | Monoclonal antibodies |
| CN101818137A (zh) | 2003-03-18 | 2010-09-01 | 诺和诺德医疗保健公司 | 生产含有gla残基的丝氨酸蛋白酶的方法 |
| EP2360171A1 (en) | 2004-12-23 | 2011-08-24 | Novo Nordisk Health Care AG | Reduction of the content of protein contaminants in compositions comprising a vitamin K-dependent protein of interest |
| AU2006320168B2 (en) | 2005-12-02 | 2012-02-23 | Wake Forest University Health Sciences | Compositions and methods for increasing production of recombinant gamma-carboxylated proteins |
| DK2125866T3 (da) | 2007-02-28 | 2013-07-29 | Baxter Int | Fremgangsmåde til rensning af rekombinant blodkoagulationsfaktor IX, som er beriget med sulfaterede og/eller phosphorylerede molekyler |
| KR101821143B1 (ko) | 2008-12-02 | 2018-01-23 | 노보 노르디스크 헬스 케어 악티엔게젤샤프트 | 폴리펩티드 정제 |
| BR112012017696A2 (pt) * | 2010-01-18 | 2017-10-03 | Novo Nordisk Healthcare Ag | Purificação de fatores de coagulação de sangue |
-
2011
- 2011-01-18 BR BR112012017696A patent/BR112012017696A2/pt not_active IP Right Cessation
- 2011-01-18 RU RU2012133474/10A patent/RU2012133474A/ru unknown
- 2011-01-18 WO PCT/EP2011/050594 patent/WO2011086197A1/en not_active Ceased
- 2011-01-18 EP EP11700547.0A patent/EP2526115B1/en not_active Not-in-force
- 2011-01-18 JP JP2012548460A patent/JP6250931B2/ja not_active Expired - Fee Related
- 2011-01-18 CN CN201180006396.5A patent/CN102939299B/zh not_active Expired - Fee Related
- 2011-01-18 KR KR1020127020380A patent/KR20120118028A/ko not_active Withdrawn
- 2011-01-18 US US13/522,548 patent/US9896677B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN102939299A (zh) | 2013-02-20 |
| US20130034896A1 (en) | 2013-02-07 |
| EP2526115A1 (en) | 2012-11-28 |
| WO2011086197A1 (en) | 2011-07-21 |
| BR112012017696A2 (pt) | 2017-10-03 |
| US9896677B2 (en) | 2018-02-20 |
| KR20120118028A (ko) | 2012-10-25 |
| EP2526115B1 (en) | 2018-08-01 |
| CN102939299B (zh) | 2016-04-20 |
| JP2013517259A (ja) | 2013-05-16 |
| JP6250931B2 (ja) | 2017-12-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| HE9A | Changing address for correspondence with an applicant | ||
| FA91 | Application withdrawn (on applicant's request) |
Effective date: 20150615 |