RU2006126329A - Белки, индуцирующие множественную устойчивость растений к фитопаногенам и вредителям - Google Patents
Белки, индуцирующие множественную устойчивость растений к фитопаногенам и вредителям Download PDFInfo
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- RU2006126329A RU2006126329A RU2006126329/13A RU2006126329A RU2006126329A RU 2006126329 A RU2006126329 A RU 2006126329A RU 2006126329/13 A RU2006126329/13 A RU 2006126329/13A RU 2006126329 A RU2006126329 A RU 2006126329A RU 2006126329 A RU2006126329 A RU 2006126329A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (10)
1. Биологически активный полипептид MF3, характеризующийся первичной структурой (аминокислотной последовательностью) SEQ ID NO.1, активный фрагмент полипептида MF3 или любое функциональное производное MF3; выше заявленный полипептид, активный фрагмент или функциональная производная способны обеспечивать эффективную устойчивость растения к инфекционным болезням и/или растительным паразитам.
2. Выделенная последовательность ДНК, изображенная в SEQ ID:2, или ее фрагмент, кодирующий функционально активный полипептид MF3 или его активный фрагмент согласно п.1; выше заявленный фрагмент ДНК может содержать вырожденные кодоны.
3. Метод приобретения устойчивости растения к фитопатогенам и/или к растительным паразитам путем введения в растение биологически активного полипептида MF3 или его активного фрагмента, или его функционального производного механически или посредством молекул-носителей.
4. Метод согласно п.3, где носителем выступает хитозан.
5. Вектор, включающий ДНК согласно п.2.
6. Метод получения трансгенного растения или культуры растительных клеток, где экспрессируется полипептид, кодируемый ДНК, использующий вектор согласно п.5.
7. Клетка организма-хозяина стабильно трансформированная или трансфецированная вектором согласно п.5.
8. Состав защитного средства растений, включающего любой из компонентов согласно п.1.
9. Активный фрагмент полипептида MF3 согласно п.1, аминокислотная последовательность которого состоит из последовательностей SEQ ID:3 или SEQ ID:4.
10. Метод выделения и очистки полипептида согласно п.1 из бактериальных клеток, экспрессирующих вышеуказанный полипептид; метод включает следующие стадии:
а) культивация бактериального штамма-продуцента и экстракция из клеток в буферном растворе при повышенной температуре;
б) осаждение неочищенного полипептида MF3 при низкой температуре с помощью осаждающего агента;
в) фракционирование растворенного осадка неочищенного полипептида MF3 с помощью анион обменной колоночной хроматографии и сбор фракций с противомикробной или инсектицидной активностью;
г) проведение электрофореза в полиакриламидном геле полипептидных фракций с противомикробной, противонематодной или инсектицидной активностями;
д) рефолдинг белка, элюированного из геля предыдущей стадии.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI20031880A FI20031880A0 (fi) | 2003-12-22 | 2003-12-22 | Kasvien vastustuskykyä patogeeneja ja tuholaisia vastaan aikaansaavat proteiinit |
| FI20031880 | 2003-12-22 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2006126329A true RU2006126329A (ru) | 2008-01-27 |
| RU2333220C2 RU2333220C2 (ru) | 2008-09-10 |
Family
ID=29763573
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2006126329/13A RU2333220C2 (ru) | 2003-12-22 | 2004-12-17 | Белки, индуцирующие множественную устойчивость растений к фитопатогенам и вредителям |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US7592509B2 (ru) |
| EP (1) | EP1711519B1 (ru) |
| JP (1) | JP4431581B2 (ru) |
| AT (1) | ATE423787T1 (ru) |
| AU (1) | AU2004303536A1 (ru) |
| DE (1) | DE602004019696D1 (ru) |
| FI (1) | FI20031880A0 (ru) |
| RU (1) | RU2333220C2 (ru) |
| WO (1) | WO2005061533A1 (ru) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3011833A1 (en) | 2014-10-23 | 2016-04-27 | Futureco Bioscience, S.A. | Bacteria with nematicidal activity and the ability to promote plant growth |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI103796B (fi) * | 1995-08-02 | 1999-09-30 | Timo Korpela | Proteiini, jolla on kasvinsuojelullisia ominaisuuksia |
-
2003
- 2003-12-22 FI FI20031880A patent/FI20031880A0/fi not_active Application Discontinuation
-
2004
- 2004-12-17 JP JP2006544475A patent/JP4431581B2/ja not_active Expired - Fee Related
- 2004-12-17 AT AT04805161T patent/ATE423787T1/de not_active IP Right Cessation
- 2004-12-17 DE DE602004019696T patent/DE602004019696D1/de active Active
- 2004-12-17 AU AU2004303536A patent/AU2004303536A1/en not_active Abandoned
- 2004-12-17 US US10/583,066 patent/US7592509B2/en not_active Expired - Fee Related
- 2004-12-17 EP EP04805161A patent/EP1711519B1/en not_active Not-in-force
- 2004-12-17 RU RU2006126329/13A patent/RU2333220C2/ru not_active IP Right Cessation
- 2004-12-17 WO PCT/FI2004/000766 patent/WO2005061533A1/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| AU2004303536A1 (en) | 2005-07-07 |
| EP1711519A1 (en) | 2006-10-18 |
| FI20031880A0 (fi) | 2003-12-22 |
| US7592509B2 (en) | 2009-09-22 |
| JP2007533303A (ja) | 2007-11-22 |
| EP1711519B1 (en) | 2009-02-25 |
| JP4431581B2 (ja) | 2010-03-17 |
| ATE423787T1 (de) | 2009-03-15 |
| RU2333220C2 (ru) | 2008-09-10 |
| US20070192898A1 (en) | 2007-08-16 |
| WO2005061533A1 (en) | 2005-07-07 |
| DE602004019696D1 (de) | 2009-04-09 |
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| Date | Code | Title | Description |
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| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20101218 |
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| RZ4A | Other changes in the information about an invention | ||
| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20121218 |