RU1808334C - Agent increasing erythrocyte deformability - Google Patents

Abstract

Usage: -the invention relates to medicine, namely to substances that increase the deformability of red blood cells. The goal is the use of the drug alpha-interferon as a substance that increases the deformability of red blood cells. SUMMARY OF THE INVENTION: A suspension of erythrocytes with an alpha-interferon Interlock preparation is incubated at a final concentration of 10-100 U / L for 0.5-1.5 hours. Positive effect: The proposed agent with high efficiency increases the deformability of red blood cells. 3 tab.

Description

The invention relates to medicine, namely to substances that increase the deformability of red blood cells.

The purpose of the invention is the use of an alpha interferon preparation as a substance that increases red blood cell deformability.

We found that this goal is achieved by incubating a suspension of red blood cells with the preparation of alpha-interferon Interloc in a final concentration of 10-100 u / l for 0.5-1.5 hours.

Previously, alpha-interferon Interlock was used as an antiviral agent. Interlock is used to treat Ophthalmic herpes (by subconjunctival injection) and cervical cancer (by injection into the cervix). In addition, interlock is known as a platelet disaggregant. Information on the use of the alpha-interferon preparation Interlock as a substance that improves erythrocyte deformability is not available in the literature.

This use of the drug interferon-alpha Interlock will solve problems such as determining the mechanism

erythrocyte plasticity disorders to determine therapeutic tactics patients in each case, predict the course of anemia and some others.

A significant difference between this proposal and the prototype is that a preparation of a different structure and pharmacological group was found that has a more pronounced property of improving the plasticity of red blood cells.

The implementation of the proposed preparation of alpha-interferon Interlock was carried out as follows. One ml of venous blood of patients stabilized with heparin was centrifuged at 3000 rpm for 10 min, which ensured the separation of blood cells. After that, 10 μl of erythrocyte sediment were added to 4 test tubes containing 5 ml of physiological saline, thus obtaining their 0.2% (by volume) suspension. The first sample was examined immediately, the other three after incubation. In one of these tubes, the drug Alpha-Interferin Interloc was added to a final concentration of 10-100 units / l, and in the second (prototype),

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olifene in a final concentration of 0.2-0.5 mg / l, in the third (control) - physiological saline in the same volume as in the two previous samples. Samples were incubated for 0.5-1.5 hours at a temperature of + 37 ° C. The deformable erythrocyte ™ was recorded using the developed methods and devices through nuclear dacron filters with a pore size of 50 μm, the test erythrocyte suspension was passed, the volumetric rate of its passage was recorded, the results were compared with the rate of passage of healthy people erythrocytes in a similar device and the deformable rate was determined ™ red blood cells.

Red blood cells were analyzed 23. patients with severe forms of purulent infection surgery. 9 of them were diagnosed with suppuration of the lungs and pleura, 7 - acute sepsis, 7-purulent peritonitis,

The results of the study are presented in table. thirteen.

From the data table. 1 it is clear that the incubation itself does not practically affect the erythrocyte deformability index, and the interferon alphainterferon drug has the ability to improve the erythrocyte deformability by 1.3 times.

In the table. 2 and 3, the selected doses of the interferon alphainterferon preparation and the incubation time with it are justified.

The drug alpha-interferon Interlbk in the selected doses and incubation modes had practically no effect on the erythrocyte deformability of 10 healthy people.

PRI me R 1. Patient G., 63 years old, who was undergoing treatment at the hospital surgery clinic CM. Kirov about acute abscess of the right lung complicated by limited pyopneumothorax (i / b No. 56817) 03/12/89, taken from a vein and stabilized with heparin 1 ml of blood. After centrifuging it at 3000 rpm for 10 minutes, four test tubes with 5 ml of physiological saline were added 10 µl of erythrocyte sediment into each tube. The first sample was examined right away,

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the other three after incubation at + 37 ° C for 0.5 hour. Before incubation, the drug alpha-interferon Interloc was added in one sample at a final concentration of 10 units / l, in the second (prototype), olifene at a final concentration of 0.3 mg / l, in the third (control), physiological solution in the same volume as in the two previous cases. After incubation, the volumetric rate of filtering samples was recorded through a nuclear laxane filter with a pore diameter of 5 μm and according to the formula

Vl - 100%,

5

0

D

V2

where D is an indicator of the deformability of red blood cells,

Va is the volumetric filtration rate of the erythrocyte suspension of 12 healthy people (it was 8.3 ml / s);

Vi is the volumetric filtration rate of the erythrocyte suspension of the analyzed samples. The deformability index of cells D was calculated and expressed in% .- The erythrocyte deformability index of patient G., respectively, was: before incubation - 24.7%, after incubation in the control - 25.5%, with interferon alpha -76, 76 1%, with olifene .61.3%.

PRI me R 2. Patient R., 63 years old, and / b No. 38762, with a diagnosis of acute right-sided pionpneumothorax, pulmonary sepsis, diabetes mellitus 11 tbsp. On 23.11.88, the deformability of red blood cells of heparinized blood was studied. The study was carried out according to a method similar to that described in example 1 with the difference that the final concentration of Interlock alphainterferon was 100 units / l, and the sample incubation time was 1.5 hours. The erythrocyte deformability index of this patient was: before incubation - 22, 3%, after incubation in the control - 21.1%, with the preparation of alpha-interferon Interlock - 78.2%, with the olefin - 59.7%.

Formulas of the invention

The use of alpha-interferon as a substance that increases the deformability of red blood cells.

Table

The effect of the drug alpha-interferon Interloc in a final concentration of 10-100 u / l

on the erythrocyte deformability of patients compared with olifen in the final

concentration of 0.2-0.5 mg / l during incubation for 0.5-1.5 hours

Table 2

Influence of the dose of the drug alpha-interferon Interlock erythrocyte formability of patients () after incubation for 0.5-1.5 hours

Table

The time of incubation with the drug interferon-alpha Interlock in a final concentration of 10-100 u / l on the deformability of red blood cells of patients / p 12 /