SU1679380A1 - Bronchial asthma differential diagnosis method - Google Patents

Abstract

The invention relates to medicine, namely to pulmonology, and can be used for the differential diagnosis of bronchial asthma. The purpose of the invention is to reduce the time of analysis. Patients are taking blood from a finger, incubated for 20 minutes in an ice bath at 2-4 ° C in the presence of a hypertonic sodium chloride solution, centrifuged, and absorbance is determined against a sample incubated with blood. with distilled water, and the number of hemolyzed erythrocytes is calculated. When their number is 28% or less, the atonic form of bronchial asthma is diagnosed, and if their content is more than 28%, it is infectious-dependent. Compared with the prototype, the diagnostic time is reduced by 1.5 times.

Description

The invention relates to medicine, namely to the field of internal diseases, in particular, pulmonology, allergology, and also pediatrics, and can be used in the examination of patients with bronchial asthma (BA) in both clinical and outpatient conditions, including follow-up.

The aim of the invention is to reduce the diagnostic time.

The method is carried out as follows.

0.02 ml of blood taken from a finger is introduced into centrifugal tube No. 1, containing 4 ml of 0.9 M sodium chloride solution, and into centrifugal tube No. 2 (control), containing 4 ml of distilled water.

Both tubes are placed for 20 min in an ice bath with a temperature of 2–4 ° C. After incubation, the tubes are centrifuged at 2000 rpm for 5 minutes.

Hemolysis of erythrocytes is judged by evaluating the supernatant in test tubes N 1 and 2 using FEC-56 (green filter). The extinction value of tubes 1 and 2 is obtained.

The content of hemolyzed erythrocytes is determined by calculating the ratio:

extinction tube number 1 1 PP 0, eccentric tube number 2 X

The proposed method was tested for the diagnosis of atonic BA in 31 patients.

Prize-winner 1 Ill B 16 years old Clinical diaphosis bronchial asgma atonic form, during the acute phase of exacerbation.

Suffering from bronchial asthma since 6 years (after suffering pneumonia). At 12 years of age, the disease became

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Compressing was repeatedly complicated by asthmatic statuses. They were hospitalized for resuscitation departments in connection with their status, and glucocorticoids were injected. She was admitted to the clinic due to an exacerbation of the disease after hypothermia (the temperature rose to 37.2 ° C due to a cold, headache, increased coughing, asphyxiation up to 4 times a day). In the hospital, after a few days, the state of health improved significantly, the attacks of asphyxiation disappeared without the use of glucocorticoids and antibiotics.

Allergic history: there are signs of epidermal dust and pollen, drug (to antibiotics) intolerance. The number of eosinophils and blood 1%. Considering the onset of the disease with an increase in temperature, the appearance of a cold, a headache, it was necessary to clarify the form of the disease. For this, a special study was conducted.

0.02 ml of blood taken from a finger was added to a centrifugal tube No. 1, containing 4 ml of a 0.9 M solution of sodium chloride, and to a centrifugal tube No. 2 (control), containing 4 ml of distilled water. Both tubes were placed for 20 min in an ice bath with a temperature of 2-4 ° C. After incubation, the tubes were centrifuged at 2000 rpm for 5 minutes.

Erythrocyte hemolysis was judged by evaluating the supernatant in tubes 1 and 2 using FEC-56 (green filter). Received the value of the extinction tube No.

1 (0.049) and N: 2 tubes (0.484). The content of hemolyzed erythrocytes was determined by calculating the ratio

 ten%.

Thus, the content of hemolyzed erythrocytes was 10%, which made it possible to diagnose the atonic form of the disease and not to prescribe antibiotics.

Example 2. Patient E. 42 years. Clinical diagnosis: bronchial asthma,

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50

five

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infectious-dependent form of medium body, remission phase.

Suffering from bronchial asthma around 1 g., When, after suffering pneumonia, the first attacks were suffocated, repeatedly examined and treated in hospitals. Was admitted to the clinic in remission to clarify the form of the disease.

Allergic history: there are signs of drug (kanamycin) intolerance, sharp odors cause difficulty breathing. The number of eosinophils in the blood of 3%. Cytological examination of sputum-eosinophils up to 14%. A special study revealed 38% of hemolyzed erythrocytes, which made it possible to diagnose an infectious-dependent form of bronchial asthma.

The positive effect of the invention compared to the prototype is to accelerate the research by 3 times (according to the prototype, the analysis time is 1.5 hours, and the proposed method is 30 minutes), which allows the use of the proposed method for express diagnostics and screening examinations.

At the same time, the volume of blood tested is reduced by a factor of 5, which makes the method suitable in pediatrics.

The method does not require expensive pharmacological preparations produced abroad.

Claims (1)

Invention Formula The method of differential diagnosis of bronchial asthma, including blood sampling, incubation in sodium chloride solution, followed by determination of hemolyzed erythrocytes, characterized in that, in order to reduce the time of diagnosis, blood is incubated in hypertonic sodium at a temperature of 2-4 ° C and with the number of hemolyzed erythrocytes equal to 28% or less, diagnose the atonic form of bronchial asthma, and with more than 28% - an infectious-dependent form of bronchial asthma.