PT2784157T - Método para conceber proteínas de ligação a rna usando o motivo ppr e sua utilização - Google Patents

Método para conceber proteínas de ligação a rna usando o motivo ppr e sua utilização

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Publication number
PT2784157T
PT2784157T PT128414356T PT12841435T PT2784157T PT 2784157 T PT2784157 T PT 2784157T PT 128414356 T PT128414356 T PT 128414356T PT 12841435 T PT12841435 T PT 12841435T PT 2784157 T PT2784157 T PT 2784157T
Authority
PT
Portugal
Prior art keywords
ppr
reason
binding proteins
designing rna
rna
Prior art date
Application number
PT128414356T
Other languages
English (en)
Portuguese (pt)
Inventor
Nakamura Takahiro
Yagi Yusuke
Kobayashi Keiko
Original Assignee
Univ Kyushu Nat Univ Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Kyushu Nat Univ Corp filed Critical Univ Kyushu Nat Univ Corp
Publication of PT2784157T publication Critical patent/PT2784157T/pt

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    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289Male sterility
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/30Detection of binding sites or motifs
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/50Mutagenesis
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10Sequence alignment; Homology search
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B35/00ICT specially adapted for in silico combinatorial libraries of nucleic acids, proteins or peptides
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16CCOMPUTATIONAL CHEMISTRY; CHEMOINFORMATICS; COMPUTATIONAL MATERIALS SCIENCE
    • G16C20/00Chemoinformatics, i.e. ICT specially adapted for the handling of physicochemical or structural data of chemical particles, elements, compounds or mixtures
    • G16C20/60In silico combinatorial chemistry
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/85Fusion polypeptide containing an RNA binding domain
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2522/00Reaction characterised by the use of non-enzymatic proteins
    • C12Q2522/10Nucleic acid binding proteins
    • C12Q2522/101Single or double stranded nucleic acid binding proteins
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Medical Informatics (AREA)
  • Evolutionary Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Library & Information Science (AREA)
  • Computing Systems (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
PT128414356T 2011-10-21 2012-10-22 Método para conceber proteínas de ligação a rna usando o motivo ppr e sua utilização PT2784157T (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2011231346 2011-10-21

Publications (1)

Publication Number Publication Date
PT2784157T true PT2784157T (pt) 2019-10-29

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Family Applications (1)

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PT128414356T PT2784157T (pt) 2011-10-21 2012-10-22 Método para conceber proteínas de ligação a rna usando o motivo ppr e sua utilização

Country Status (8)

Country Link
US (7) US9513283B2 (enExample)
EP (2) EP2784157B1 (enExample)
JP (9) JP6164488B2 (enExample)
AU (5) AU2012326971C1 (enExample)
DK (1) DK2784157T3 (enExample)
ES (1) ES2751126T3 (enExample)
PT (1) PT2784157T (enExample)
WO (1) WO2013058404A1 (enExample)

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JP6164488B2 (ja) 2011-10-21 2017-07-19 国立大学法人九州大学 Pprモチーフを利用したrna結合性蛋白質の設計方法及びその利用
CA2873073A1 (en) * 2012-04-16 2013-10-24 The University Of Western Australia Peptides for the binding of nucleotide targets
NZ714264A (en) * 2013-04-22 2020-10-30 Univ Kyushu Nat Univ Corp Dna-binding protein using ppr motif, and use thereof
SG11201609211VA (en) 2014-03-05 2016-12-29 Nat Univ Corp Univ Kobe Genomic sequence modification method for specifically converting nucleic acid bases of targeted dna sequence, and molecular complex for use in same
JP6664693B2 (ja) 2015-09-09 2020-03-13 国立大学法人神戸大学 標的化したdna配列の核酸塩基を特異的に変換する、グラム陽性菌のゲノム配列の変換方法、及びそれに用いる分子複合体
ES2930643T3 (es) 2015-11-23 2022-12-20 Univ California Seguimiento y manipulación de ARN celular a través de la administración nuclear de CRISPR/CAS9
KR102061438B1 (ko) 2015-11-27 2019-12-31 고쿠리츠다이가쿠호진 고베다이가쿠 표적화 dna 서열 중의 핵산 염기가 특이적으로 전환되어 있는 단자엽식물 게놈 서열을 전환시키는 방법, 및 그것에 사용되는 분자 복합체
KR102407776B1 (ko) 2016-06-03 2022-06-10 고쿠리쓰다이가쿠호진 규슈다이가쿠 표적 mRNA로부터의 단백질 발현량을 향상시키기 위한 융합 단백질
US11136361B2 (en) 2016-06-03 2021-10-05 Kyushu University, National University Corporation Fusion protein for improving protein expression from target mRNA
CN109563137A (zh) * 2016-08-10 2019-04-02 富士胶片和光纯药株式会社 利用了ppr基序的dna结合性蛋白质及其应用
WO2018208998A1 (en) 2017-05-10 2018-11-15 The Regents Of The University Of California Directed editing of cellular rna via nuclear delivery of crispr/cas9
CN108932400B (zh) * 2017-05-24 2021-07-23 北京工业大学 一种考虑界面信息的有效的蛋白质-rna复合物结构预测方法
CN108959852B (zh) * 2017-05-24 2021-12-24 北京工业大学 基于氨基酸-核苷酸成对偏好性信息的蛋白质上与rna结合模块的预测方法
US20200071718A1 (en) 2018-06-08 2020-03-05 Locana, Inc. Rna-targeting fusion protein compositions and methods for use
KR102898966B1 (ko) 2019-05-29 2025-12-10 고쿠리쓰다이가쿠호진 규슈다이가쿠 응집이 적은 피피알 단백질 및 그의 이용
KR20220023985A (ko) 2019-05-29 2022-03-03 에딧포스 인코포레이티드 효율적인 피피알 단백질의 작제방법 및 그의 이용
CN114450031A (zh) 2019-07-10 2022-05-06 洛卡纳生物股份有限公司 靶向rna的敲低和替代组合物及使用方法
KR20220165747A (ko) * 2020-03-31 2022-12-15 에딧포스 인코포레이티드 표적 rna의 편집방법
JP2023551873A (ja) 2020-12-01 2023-12-13 ロックアネイビオ, インコーポレイテッド Cagリピート病を処置するためのrna標的化組成物および方法
JP2023551874A (ja) 2020-12-01 2023-12-13 ロックアネイビオ, インコーポレイテッド 筋強直性ジストロフィー1型を処置するためのrna標的化組成物および方法
WO2022221278A1 (en) 2021-04-12 2022-10-20 Locanabio, Inc. Compositions and methods comprising hybrid promoters
WO2022226375A1 (en) 2021-04-23 2022-10-27 Locanabio, Inc. Tissue-targeted modified aav capsids and methods of use thereof
JP2024514956A (ja) 2021-04-23 2024-04-03 ロックアネイビオ, インコーポレイテッド 組織標的化された改変aavカプシドおよびその使用方法
BR112023022129A2 (pt) 2021-04-30 2024-01-09 Editforce Inc Fármaco terapêutico para distrofia miotônica tipo 1
JP7125727B1 (ja) * 2021-09-07 2022-08-25 国立大学法人千葉大学 核酸配列改変用組成物および核酸配列の標的部位を改変する方法
CN116888671A (zh) * 2021-09-29 2023-10-13 京东方科技集团股份有限公司 Rna-蛋白质相互作用预测方法、装置、介质及电子设备
WO2023154807A2 (en) 2022-02-09 2023-08-17 Locanabio, Inc. Compositions and methods for modulating pre-mrna splicing

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CA2445700C (en) 2001-04-25 2012-04-17 Mitsubishi Chemical Corporation Protein involved in restoration of cytoplasmic male sterility to fertility and gene encoding the protein and gene
JP2002355041A (ja) * 2001-04-25 2002-12-10 Mitsubishi Chemicals Corp 細胞質雄性不稔から可稔への回復に関与する遺伝子
JP4102099B2 (ja) 2001-04-25 2008-06-18 アンスティテュ ナシオナル ドゥ ラ ルシェルシュ アグロノミック 細胞質雄性不稔から可稔への回復に関与するタンパク質及びそれをコードする遺伝子
US20040117868A1 (en) 2002-01-29 2004-06-17 Jun Imamura Protein participating in restoration from cytoplasmic male sterility to fertility and gene encoding the same
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US8344122B2 (en) 2008-03-12 2013-01-01 Tohoku University Fertility restorer gene and fertility restoration method for CW-type male sterile cytoplasm of rice
ES2696825T3 (es) 2009-12-10 2019-01-18 Univ Minnesota Modificación del ADN inducida por el efector TAL
JP2013128413A (ja) 2010-03-11 2013-07-04 Kyushu Univ Pprモチーフを利用したrna結合性蛋白質の改変方法
JP6164488B2 (ja) * 2011-10-21 2017-07-19 国立大学法人九州大学 Pprモチーフを利用したrna結合性蛋白質の設計方法及びその利用
CA2873073A1 (en) * 2012-04-16 2013-10-24 The University Of Western Australia Peptides for the binding of nucleotide targets

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US9984202B2 (en) 2018-05-29
JP2017192391A (ja) 2017-10-26
US10943671B2 (en) 2021-03-09
AU2017254874B2 (en) 2019-09-12
JP7590019B2 (ja) 2024-11-26
JP2018042569A (ja) 2018-03-22
ES2751126T3 (es) 2020-03-30
JP6454398B2 (ja) 2019-01-16
AU2019280013A1 (en) 2020-01-16
AU2019280013B2 (en) 2022-03-31
DK2784157T3 (da) 2019-10-21
JP2024010112A (ja) 2024-01-23
JP2025020327A (ja) 2025-02-12
JP2018078896A (ja) 2018-05-24
AU2025204996A1 (en) 2025-07-24
US20140335521A1 (en) 2014-11-13
US20210249103A1 (en) 2021-08-12
US20200294626A1 (en) 2020-09-17
US20180247018A1 (en) 2018-08-30
EP2784157A1 (en) 2014-10-01
EP2784157B1 (en) 2019-09-18
JP7381133B2 (ja) 2023-11-15
AU2012326971A1 (en) 2014-06-12
US20240047005A1 (en) 2024-02-08
AU2012326971C1 (en) 2018-02-08
JP6934644B2 (ja) 2021-09-15
JP7157483B2 (ja) 2022-10-20
US20170124252A1 (en) 2017-05-04
US20190311783A1 (en) 2019-10-10
AU2022204654B2 (en) 2025-04-03
US10679731B2 (en) 2020-06-09
JP2021130678A (ja) 2021-09-09
AU2012326971B2 (en) 2017-10-19
US10340028B2 (en) 2019-07-02
EP3611261A1 (en) 2020-02-19
JP2022177289A (ja) 2022-11-30
JP6164488B2 (ja) 2017-07-19
EP2784157A4 (en) 2015-07-01
JP2017148078A (ja) 2017-08-31
JP6270192B2 (ja) 2018-01-31
WO2013058404A1 (ja) 2013-04-25
JPWO2013058404A1 (ja) 2015-04-02
US11742056B2 (en) 2023-08-29
JP7789426B2 (ja) 2025-12-22
JP6267388B2 (ja) 2018-01-24
AU2022204654A1 (en) 2022-07-21
US9513283B2 (en) 2016-12-06
AU2017254874A1 (en) 2017-11-16

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