PT1718745E - Produção de genes recombinantes em células procarióticas através da utilização de dois elementos extracromossomais - Google Patents

Produção de genes recombinantes em células procarióticas através da utilização de dois elementos extracromossomais Download PDF

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Publication number
PT1718745E
PT1718745E PT05715582T PT05715582T PT1718745E PT 1718745 E PT1718745 E PT 1718745E PT 05715582 T PT05715582 T PT 05715582T PT 05715582 T PT05715582 T PT 05715582T PT 1718745 E PT1718745 E PT 1718745E
Authority
PT
Portugal
Prior art keywords
prokaryotic cells
generation
extrachromosomal elements
recombinant genes
methods
Prior art date
Application number
PT05715582T
Other languages
English (en)
Inventor
Miroslav Radman
Ivan Matic
Ana Rodriguez-Gomez
Tatjana Galic
Marie-Agnes Petit
Original Assignee
Mixis France Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mixis France Sa filed Critical Mixis France Sa
Publication of PT1718745E publication Critical patent/PT1718745E/pt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1024In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
PT05715582T 2004-02-26 2005-02-26 Produção de genes recombinantes em células procarióticas através da utilização de dois elementos extracromossomais PT1718745E (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP04360018 2004-02-26

Publications (1)

Publication Number Publication Date
PT1718745E true PT1718745E (pt) 2009-01-27

Family

ID=34896166

Family Applications (1)

Application Number Title Priority Date Filing Date
PT05715582T PT1718745E (pt) 2004-02-26 2005-02-26 Produção de genes recombinantes em células procarióticas através da utilização de dois elementos extracromossomais

Country Status (19)

Country Link
US (1) US20070212692A1 (pt)
EP (1) EP1718745B1 (pt)
JP (1) JP4777969B2 (pt)
KR (1) KR20060126570A (pt)
CN (1) CN1946844B (pt)
AT (1) ATE414770T1 (pt)
AU (2) AU2005217093B2 (pt)
CA (1) CA2557490A1 (pt)
DE (1) DE602005011106D1 (pt)
DK (1) DK1718745T3 (pt)
EA (1) EA012070B1 (pt)
ES (1) ES2315849T3 (pt)
HK (1) HK1097294A1 (pt)
HR (1) HRP20090023T3 (pt)
NZ (1) NZ548686A (pt)
PT (1) PT1718745E (pt)
SI (1) SI1718745T1 (pt)
WO (1) WO2005083079A1 (pt)
ZA (1) ZA200606110B (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105200074B (zh) * 2015-09-21 2019-02-22 华侨大学 一种利用dna非特异性结合蛋白hu蛋白构建克隆载体的方法
JP7259131B2 (ja) * 2019-08-05 2023-04-17 ワッカー ケミー アクチエンゲゼルシャフト 発酵法で組換えタンパク質を放出する細菌株

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1274008A (zh) * 2000-03-03 2000-11-22 北京大学 枯草芽孢杆菌杀菌肽的体外表达及其方法

Also Published As

Publication number Publication date
EA012070B1 (ru) 2009-08-28
JP2007523656A (ja) 2007-08-23
DK1718745T3 (da) 2009-02-09
EP1718745B1 (en) 2008-11-19
AU2005217093B2 (en) 2009-01-08
ZA200606110B (en) 2008-02-27
CA2557490A1 (en) 2005-09-09
HRP20090023T3 (en) 2009-02-28
SI1718745T1 (sl) 2009-04-30
AU2008264152A1 (en) 2009-01-29
WO2005083079A1 (en) 2005-09-09
ATE414770T1 (de) 2008-12-15
CN1946844B (zh) 2012-02-29
JP4777969B2 (ja) 2011-09-21
US20070212692A1 (en) 2007-09-13
ES2315849T3 (es) 2009-04-01
AU2005217093A1 (en) 2005-09-09
KR20060126570A (ko) 2006-12-07
EA200601561A1 (ru) 2006-12-29
HK1097294A1 (en) 2007-06-22
EP1718745A1 (en) 2006-11-08
CN1946844A (zh) 2007-04-11
NZ548686A (en) 2009-10-30
DE602005011106D1 (de) 2009-01-02

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