PL419487A1 - Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting it - Google Patents
Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting itInfo
- Publication number
- PL419487A1 PL419487A1 PL419487A PL41948716A PL419487A1 PL 419487 A1 PL419487 A1 PL 419487A1 PL 419487 A PL419487 A PL 419487A PL 41948716 A PL41948716 A PL 41948716A PL 419487 A1 PL419487 A1 PL 419487A1
- Authority
- PL
- Poland
- Prior art keywords
- detecting
- fungal pathogen
- puccinia recondita
- oligonucleotide primers
- pathogen puccinia
- Prior art date
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Zgłoszenie dotyczy oligonukleotydów, umożliwiających stwierdzanie obecności materiału genetycznego patogenu grzybowego Puccinia recondita w badanym materiale biologicznym z wykorzystaniem łańcuchowej reakcji polimerazy lub jej modyfikacji. Przedmiotem zgłoszenia jest para specyficznych gatunkowo starterów oligonukleotydowych do zastosowania w reakcji łańcuchowej polimerazy lub jej modyfikacji, o sekwencjach: LidPr11: 5'-GACAACGAAGCCCTCTACGA-3' LidPr12: 5' -GGTGCGATGTCACAATGAGC-3' komplementarnych do specyficznych gatunkowo fragmentów sekwencji genomu Puccinia recondita. Zgłoszenie obejmuje też sposób wykrywania patogenu grzybowego pszenicy Puccinia recondita, w którym dochodzi do amplifikacji określonego fragmentu DNA o długości 530 - 570 par zasad w reakcji PCR z zastosowaniem pary starterów oligonukleotydowych.The application relates to oligonucleotides, enabling the presence of the genetic material of the fungal pathogen Puccinia recondita to be tested in biological material using the polymerase chain reaction or its modification. The subject of the application is a pair of species-specific oligonucleotide primers for use in the polymerase chain reaction or modification thereof, with the sequences: LidPr11: 5'-GACAACGAAGCCCTCTACGA-3 'LidPr12: 5' -GGTGCGATGTCACAATGAGC-3 'complementary to the genome specific species. The application also includes a method of detecting the wheat fungal pathogen Puccinia recondita in which a specific 530-570 base pair DNA fragment is amplified in a PCR reaction using an oligonucleotide primer pair.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PL419487A PL232093B1 (en) | 2016-11-17 | 2016-11-17 | Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting it |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PL419487A PL232093B1 (en) | 2016-11-17 | 2016-11-17 | Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting it |
Publications (2)
Publication Number | Publication Date |
---|---|
PL419487A1 true PL419487A1 (en) | 2018-05-21 |
PL232093B1 PL232093B1 (en) | 2019-05-31 |
Family
ID=62142467
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PL419487A PL232093B1 (en) | 2016-11-17 | 2016-11-17 | Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting it |
Country Status (1)
Country | Link |
---|---|
PL (1) | PL232093B1 (en) |
-
2016
- 2016-11-17 PL PL419487A patent/PL232093B1/en unknown
Also Published As
Publication number | Publication date |
---|---|
PL232093B1 (en) | 2019-05-31 |
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