PL419487A1 - Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting it - Google Patents

Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting it

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Publication number
PL419487A1
PL419487A1 PL419487A PL41948716A PL419487A1 PL 419487 A1 PL419487 A1 PL 419487A1 PL 419487 A PL419487 A PL 419487A PL 41948716 A PL41948716 A PL 41948716A PL 419487 A1 PL419487 A1 PL 419487A1
Authority
PL
Poland
Prior art keywords
detecting
fungal pathogen
puccinia recondita
oligonucleotide primers
pathogen puccinia
Prior art date
Application number
PL419487A
Other languages
Polish (pl)
Other versions
PL232093B1 (en
Inventor
Adam Kuzdraliński
Anna Kot
Hubert Szczerba
Michał Nowak
Paweł Muzyka
Michał Lechowski
Agnieszka Ostrowska
Marta Muszyńska
Zdzisław TARGOŃSKI
Original Assignee
Uniwersytet Przyrodniczy W Lublinie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Uniwersytet Przyrodniczy W Lublinie filed Critical Uniwersytet Przyrodniczy W Lublinie
Priority to PL419487A priority Critical patent/PL232093B1/en
Publication of PL419487A1 publication Critical patent/PL419487A1/en
Publication of PL232093B1 publication Critical patent/PL232093B1/en

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Abstract

Zgłoszenie dotyczy oligonukleotydów, umożliwiających stwierdzanie obecności materiału genetycznego patogenu grzybowego Puccinia recondita w badanym materiale biologicznym z wykorzystaniem łańcuchowej reakcji polimerazy lub jej modyfikacji. Przedmiotem zgłoszenia jest para specyficznych gatunkowo starterów oligonukleotydowych do zastosowania w reakcji łańcuchowej polimerazy lub jej modyfikacji, o sekwencjach: LidPr11: 5'-GACAACGAAGCCCTCTACGA-3' LidPr12: 5' -GGTGCGATGTCACAATGAGC-3' komplementarnych do specyficznych gatunkowo fragmentów sekwencji genomu Puccinia recondita. Zgłoszenie obejmuje też sposób wykrywania patogenu grzybowego pszenicy Puccinia recondita, w którym dochodzi do amplifikacji określonego fragmentu DNA o długości 530 - 570 par zasad w reakcji PCR z zastosowaniem pary starterów oligonukleotydowych.The application relates to oligonucleotides, enabling the presence of the genetic material of the fungal pathogen Puccinia recondita to be tested in biological material using the polymerase chain reaction or its modification. The subject of the application is a pair of species-specific oligonucleotide primers for use in the polymerase chain reaction or modification thereof, with the sequences: LidPr11: 5'-GACAACGAAGCCCTCTACGA-3 'LidPr12: 5' -GGTGCGATGTCACAATGAGC-3 'complementary to the genome specific species. The application also includes a method of detecting the wheat fungal pathogen Puccinia recondita in which a specific 530-570 base pair DNA fragment is amplified in a PCR reaction using an oligonucleotide primer pair.

PL419487A 2016-11-17 2016-11-17 Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting it PL232093B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL419487A PL232093B1 (en) 2016-11-17 2016-11-17 Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting it

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL419487A PL232093B1 (en) 2016-11-17 2016-11-17 Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting it

Publications (2)

Publication Number Publication Date
PL419487A1 true PL419487A1 (en) 2018-05-21
PL232093B1 PL232093B1 (en) 2019-05-31

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Family Applications (1)

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PL419487A PL232093B1 (en) 2016-11-17 2016-11-17 Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting it

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PL (1) PL232093B1 (en)

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Publication number Publication date
PL232093B1 (en) 2019-05-31

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