Державний Науково-Контрольний Інститут Біотехнології І Штамів Мікроорганізмів
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Measuring Or Testing Involving Enzymes Or Micro-Organisms
(AREA)
Abstract
The method of detecting of DNA bacteria YERSINIA ENTEROCOLITICA by polymerase chain reaction involving the detection in the samples of specific fragments of nucleic acid (DNA) of the pathogen through "half nidicolous" version of the polymerase chain reaction (PCR) - enzyme reaction and three artificially synthesized oligonucleotide primers that allow multiple copy specific DNA segments infectious agent under certain temperature and time parameters and the number of cycles and for "half nidicolous" option PCR carried out two stages of reaction, using each stage a pair of primers in the first stage using a pair of oligonucleotide primers with the following nucleotide sequence: 16SYOF1 (5'-TAGTAGGTGGGGTAATGGCTC-3 ') O16SmdR1 (5'-CCTCCTCGCTGAAAGTGCT-3 '), the second stage use a pair of artificially synthesized oligonucleotide primers with the following nucleotide sequence: 16SYOF1 (5'-TAGTAGGTGGGGTAATGGCTC-3 ') 16SYOR1 (5'-gTAACgTCAATCCAACAACCTAT-3 '), length fragment of DNA synthesized - 191 n. n.
UAU201501585U2015-02-242015-02-24Method of detecting of dna bacteria yersinia enterocolitica by polymerase chain reaction
UA103102U
(en)
METHOD OF CONVERTING GENOME SEQUENCE FROM GRAM-POSITIVE BACTERIA BY SPECIFICALLY CONVERTING NUCLEIC ACID BASE INTO THE INTENDED DNA SEQUENCE, AND MOLECULAR COMPLEX USED THEREFOR
Method for instant exclusion of salmonellosis and genetic typing of salmonella enterica enteritidis and salmonella enterica typhimurium with multiplex pcr
Method for the detection of dna of bacteria chlamydia abortus, chlamydia pecorum, chlamydia psittaci in polymerase chain reaction by amplification of gene fragment encoding endoribonuclease p (rnase p rna)