PL419486A1 - Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting it - Google Patents

Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting it

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Publication number
PL419486A1
PL419486A1 PL419486A PL41948616A PL419486A1 PL 419486 A1 PL419486 A1 PL 419486A1 PL 419486 A PL419486 A PL 419486A PL 41948616 A PL41948616 A PL 41948616A PL 419486 A1 PL419486 A1 PL 419486A1
Authority
PL
Poland
Prior art keywords
detecting
fungal pathogen
puccinia recondita
oligonucleotide primers
species
Prior art date
Application number
PL419486A
Other languages
Polish (pl)
Other versions
PL232092B1 (en
Inventor
Adam Kuzdraliński
Anna Kot
Hubert Szczerba
Michał Nowak
Paweł Muzyka
Michał Lechowski
Agnieszka Ostrowska
Marta Muszyńska
Zdzisław TARGOŃSKI
Original Assignee
Uniwersytet Przyrodniczy W Lublinie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Uniwersytet Przyrodniczy W Lublinie filed Critical Uniwersytet Przyrodniczy W Lublinie
Priority to PL419486A priority Critical patent/PL232092B1/en
Publication of PL419486A1 publication Critical patent/PL419486A1/en
Publication of PL232092B1 publication Critical patent/PL232092B1/en

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Abstract

Zgłoszenie dotyczy oligonukleotydów, umożliwiających stwierdzanie obecności materiału genetycznego patogenu grzybowego Puccinia recondita w badanym materiale biologicznym z wykorzystaniem reakcji polimerazy lub jej modyfikacji. Przedmiotem zgłoszenia jest para specyficznych gatunkowo starterów oligonukleotydowych do zastosowania w reakcji łańcuchowej polimerazy lub jej modyfikacji, o sekwencjach: LidPrl5: 5'- ACGAAGCCCTCTACGACATC-3' LidPr16: 5'- GTCTGAGGCAGCCATCATGT-3' komplementarnych do specyficznych gatunkowo fragmentów sekwencji genomu Puccinia recondita. Zgłoszenie obejmuje też sposób wykrywania patogenu grzybowego pszenicy Puccinia recondita, w którym dochodzi do amplifikacji określonego fragmentu DNA o długości 280 - 330 par zasad w reakcji PCR z zastosowaniem pary starterów oligonukleotydowych.The application relates to oligonucleotides enabling the presence of the genetic material of the fungal pathogen Puccinia recondita to be tested in the biological material using the polymerase reaction or its modification. The subject of the application is a pair of species-specific oligonucleotide primers for use in the polymerase chain reaction or its modification, with the sequences: LidPrl5: 5'- ACGAAGCCCTCTACGACATC-3 'LidPr16: 5'-GTCTGAGGCAGCCATCATGT-3' complementary to species-specific receptin species. The application also includes a method of detection of the wheat fungal pathogen Puccinia recondita in which a specific DNA fragment of 280 - 330 base pairs is amplified in a PCR reaction using an oligonucleotide primer pair.

PL419486A 2016-11-17 2016-11-17 Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting it PL232092B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL419486A PL232092B1 (en) 2016-11-17 2016-11-17 Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting it

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL419486A PL232092B1 (en) 2016-11-17 2016-11-17 Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting it

Publications (2)

Publication Number Publication Date
PL419486A1 true PL419486A1 (en) 2018-05-21
PL232092B1 PL232092B1 (en) 2019-05-31

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Family Applications (1)

Application Number Title Priority Date Filing Date
PL419486A PL232092B1 (en) 2016-11-17 2016-11-17 Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting it

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PL (1) PL232092B1 (en)

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Publication number Publication date
PL232092B1 (en) 2019-05-31

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