PL419488A1 - Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it - Google Patents

Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it

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Publication number
PL419488A1
PL419488A1 PL419488A PL41948816A PL419488A1 PL 419488 A1 PL419488 A1 PL 419488A1 PL 419488 A PL419488 A PL 419488A PL 41948816 A PL41948816 A PL 41948816A PL 419488 A1 PL419488 A1 PL 419488A1
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PL
Poland
Prior art keywords
detecting
oligonucleotide primers
fungal pathogen
blumeria graminis
application
Prior art date
Application number
PL419488A
Other languages
Polish (pl)
Other versions
PL230742B1 (en
Inventor
Adam Kuzdraliński
Hubert Szczerba
Anna Kot
Agnieszka Ostrowska
Michał Nowak
Marta Muszyńska
Zdzisław TARGOŃSKI
Original Assignee
Uniwersytet Przyrodniczy W Lublinie
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Publication date
Application filed by Uniwersytet Przyrodniczy W Lublinie filed Critical Uniwersytet Przyrodniczy W Lublinie
Priority to PL419488A priority Critical patent/PL230742B1/en
Publication of PL419488A1 publication Critical patent/PL419488A1/en
Publication of PL230742B1 publication Critical patent/PL230742B1/en

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Abstract

Zgłoszenie dotyczy oligonukleotydów, umożliwiających stwierdzanie obecności materiału genetycznego patogenu grzybowego Blumeria graminis w badanym materiale biologicznym z wykorzystaniem łańcuchowej reakcji polimerazy lub jej modyfikacji. Przedmiot zgłoszenia stanowi para specyficznych gatunkowo starterów oligonukleotydowych do zastosowania w reakcji łańcuchowej polimerazy lub jej modyfikacji, o sekwencjach: LidBg21: 5'-AATTCGGCTTTAGCATTGCGTT-3' LidBg22: 5'-TTCGTGTTCCCCAGAATATATCA-3' komplementarnych do specyficznych gatunkowo fragmentów sekwencji genomu Blumeria graminis. Zgłoszenie obejmuje też sposób wykrywania patogenu grzybowego pszenicy Blumeria graminis, w którym dochodzi do amplifikacji określonego fragmentu DNA o długości 220 - 260 par zasad w reakcji PCR z zastosowaniem pary starterów oligonukleotydowych.The application relates to oligonucleotides enabling the presence of the genetic material of the fungal pathogen Blumeria graminis to be determined in the biological material tested using the polymerase chain reaction or its modification. The subject of the application is a pair of species-specific oligonucleotide primers for use in the polymerase chain reaction or its modification, with the sequences: LidBg21: 5'-AATTCGGCTTTAGCATTGCGTT-3 'LidBg22: 5'-TTCGTGTTCCCCAGAATATATCA-3' of species-specific complementary genomes. The application also includes a method of detecting the fungal pathogen of wheat Blumeria graminis, in which a specific fragment of DNA with a length of 220 - 260 base pairs is amplified in a PCR reaction using a pair of oligonucleotide primers.

PL419488A 2016-11-17 2016-11-17 Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it PL230742B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL419488A PL230742B1 (en) 2016-11-17 2016-11-17 Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL419488A PL230742B1 (en) 2016-11-17 2016-11-17 Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it

Publications (2)

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PL419488A1 true PL419488A1 (en) 2018-05-21
PL230742B1 PL230742B1 (en) 2018-12-31

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Family Applications (1)

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PL419488A PL230742B1 (en) 2016-11-17 2016-11-17 Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it

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PL (1) PL230742B1 (en)

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Publication number Publication date
PL230742B1 (en) 2018-12-31

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