PL419483A1 - Oligonucleotide primers for detecting wheat fungal pathogen Puccinia striiformis and method for detecting it - Google Patents

Oligonucleotide primers for detecting wheat fungal pathogen Puccinia striiformis and method for detecting it

Info

Publication number
PL419483A1
PL419483A1 PL419483A PL41948316A PL419483A1 PL 419483 A1 PL419483 A1 PL 419483A1 PL 419483 A PL419483 A PL 419483A PL 41948316 A PL41948316 A PL 41948316A PL 419483 A1 PL419483 A1 PL 419483A1
Authority
PL
Poland
Prior art keywords
detecting
oligonucleotide primers
fungal pathogen
puccinia striiformis
application
Prior art date
Application number
PL419483A
Other languages
Polish (pl)
Other versions
PL232070B1 (en
Inventor
Adam Kuzdraliński
Anna Kot
Hubert Szczerba
Michał Nowak
Paweł Muzyka
Michał Lechowski
Agnieszka Ostrowska
Marta Muszyńska
Zdzisław TARGOŃSKI
Original Assignee
Uniwersytet Przyrodniczy W Lublinie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Przyrodniczy W Lublinie filed Critical Uniwersytet Przyrodniczy W Lublinie
Priority to PL419483A priority Critical patent/PL232070B1/en
Publication of PL419483A1 publication Critical patent/PL419483A1/en
Publication of PL232070B1 publication Critical patent/PL232070B1/en

Links

Landscapes

  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Zgłoszenie dotyczy oligonukleotydów, umożliwiających stwierdzanie obecności materiału genetycznego patogenu grzybowego Puccinia striiformis w badanym materiale biologicznym z wykorzystaniem łańcuchowej reakcji polimerazy lub jej modyfikacji. Przedmiot zgłoszenia stanowi para specyficznych gatunkowo starterów oligonukleotydowych do zastosowania w reakcji łańcuchowej polimerazy lub jej modyfikacji, o sekwencjach: LidPs11: 5'TTACGACATCTGCTTCCGCA 3' LidPs12: 5' TGCGATGTCAACTCTGGGAC 3' komplementarnych do specyficznych gatunkowo fragmentów sekwencji genomu Puccinia striiformis. Zgłoszenie obejmuje też sposób wykrywania patogenu grzybowego pszenicy Puccinia striiformis, w którym dochodzi do amplifikacji określonego fragmentu DNA o długości 230 - 270 par zasad w reakcji PCR z zastosowaniem pary starterów oligonukleotydowych.The application relates to oligonucleotides enabling the presence of the genetic material of the fungal pathogen Puccinia striiformis to be tested in the biological material tested using the polymerase chain reaction or its modification. The subject of the application is a pair of species-specific oligonucleotide primers for use in the polymerase chain reaction or its modification, with the sequences: LidPs11: 5'TTACGACATCTGCTTCCGCA 3 'LidPs12: 5' TGCGATGTCAACTCTGGGAC 3 'complementary to the species-specific fragment of the genomic stain sequence. The application also includes a method of detecting the fungal pathogen of wheat Puccinia striiformis, in which a specific fragment of DNA with a length of 230 - 270 base pairs is amplified in a PCR reaction using a pair of oligonucleotide primers.

PL419483A 2016-11-17 2016-11-17 Oligonucleotide primers for detecting wheat fungal pathogen Puccinia striiformis and method for detecting it PL232070B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL419483A PL232070B1 (en) 2016-11-17 2016-11-17 Oligonucleotide primers for detecting wheat fungal pathogen Puccinia striiformis and method for detecting it

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL419483A PL232070B1 (en) 2016-11-17 2016-11-17 Oligonucleotide primers for detecting wheat fungal pathogen Puccinia striiformis and method for detecting it

Publications (2)

Publication Number Publication Date
PL419483A1 true PL419483A1 (en) 2018-05-21
PL232070B1 PL232070B1 (en) 2019-05-31

Family

ID=62142450

Family Applications (1)

Application Number Title Priority Date Filing Date
PL419483A PL232070B1 (en) 2016-11-17 2016-11-17 Oligonucleotide primers for detecting wheat fungal pathogen Puccinia striiformis and method for detecting it

Country Status (1)

Country Link
PL (1) PL232070B1 (en)

Also Published As

Publication number Publication date
PL232070B1 (en) 2019-05-31

Similar Documents

Publication Publication Date Title
JP2016195614A5 (en)
SA516371691B1 (en) Methods and compositions for dna profiling
WO2015123430A3 (en) Single molecule electronic multiplex snp assay and pcr analysis
NZ723570A (en) Compositions and methods for quantifying a nucleic acid sequence in a sample
WO2015114469A3 (en) Covered sequence conversion dna and detection methods
MX2017001405A (en) Detection of target nucleic acids using hybridization.
MX2018014163A (en) Labeled nucleotide compositions and methods for nucleic acid sequencing.
GB2519465A (en) Solid matrix for one step nucleic acid amplification
EA201491821A1 (en) MODIFIED oligonucleotides, including thiol functional groups, and their use for the detection of nucleic acids
PL419483A1 (en) Oligonucleotide primers for detecting wheat fungal pathogen Puccinia striiformis and method for detecting it
PL419484A1 (en) Oligonucleotide primers for detecting wheat fungal pathogen Puccinia striiformis and method for detecting it
PL419482A1 (en) Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it
PL419488A1 (en) Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it
PL419487A1 (en) Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting it
PL419485A1 (en) Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting it
PL419489A1 (en) Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it
PL419486A1 (en) Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting it
MX2019011503A (en) Primer compositions and multiplex methods for detecting the presence of listeria.
PL419494A1 (en) Oligonucleotide primers for detecting wheat fungal pathogen Puccinia striiformis and method for detecting it
PL431170A1 (en) Oligonucleotide primers hybridizing within the Cyp51 gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection
UA103102U (en) Method of detecting of dna bacteria yersinia enterocolitica by polymerase chain reaction
AR109017A1 (en) METHODS FOR USING LONG SINGLE CHAIN DNA POLYUCLEOTIDES AS PRIMERS IN PCR TESTS
RU2010127614A (en) METHOD FOR IDENTIFICATION OF MYCOBACTERIA USING POLYMERASE CHAIN REACTION
RU2014107633A (en) METHOD FOR IDENTIFICATION OF THE p.Q368X MUTATION IN THE MYOCYLINE GENE (MYOC), CAUSING THE DEVELOPMENT OF PRIMARY OPEN-ANGLE GLAUCOMA
RU2015116726A (en) A SET OF SYNTHETIC OLIGODESOXYRIBONUCLEOTIDES FOR AMPLIFICATION AND DETECTION OF ENDOGENOUS INTERNAL CONTROL IN RESEARCH OF BIOLOGICAL MATERIAL OF CEREUMATIC RESEARCH