PL419482A1 - Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it - Google Patents

Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it

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Publication number
PL419482A1
PL419482A1 PL419482A PL41948216A PL419482A1 PL 419482 A1 PL419482 A1 PL 419482A1 PL 419482 A PL419482 A PL 419482A PL 41948216 A PL41948216 A PL 41948216A PL 419482 A1 PL419482 A1 PL 419482A1
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PL
Poland
Prior art keywords
detecting
blumeria graminis
oligonucleotide primers
fungal pathogen
application
Prior art date
Application number
PL419482A
Other languages
Polish (pl)
Other versions
PL230741B1 (en
Inventor
Adam Kuzdraliński
Hubert Szczerba
Anna Kot
Agnieszka Ostrowska
Michał Nowak
Marta Muszyńska
Zdzisław TARGOŃSKI
Original Assignee
Uniwersytet Przyrodniczy W Lublinie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Uniwersytet Przyrodniczy W Lublinie filed Critical Uniwersytet Przyrodniczy W Lublinie
Priority to PL419482A priority Critical patent/PL230741B1/en
Publication of PL419482A1 publication Critical patent/PL419482A1/en
Publication of PL230741B1 publication Critical patent/PL230741B1/en

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Abstract

Zgłoszenie dotyczy oligonukleotydów, umożliwiających stwierdzanie obecności materiału genetycznego patogenu grzybowego Blumeria graminis w badanym materiale biologicznym z wykorzystaniem łańcuchowej reakcji polimerazy lub jej modyfikacji. Przedmiot zgłoszenia stanowi para specyficznych gatunkowo starterów oligonukleotydowych do zastosowania w reakcji łańcuchowej polimerazy lub jej modyfikacji, o sekwencjach: LidBgl3: 5'-CGAGGTATCCGCAAYCATCA-3' LidBgl4: 5'-GACGAGAACAGCACGAGGAA-3' komplementarnych do specyficznych gatunkowo fragmentów sekwencji genomu. Przedmiotem zgłoszenia jest też Blumeria graminis sposób wykrywania patogenu grzybowego pszenicy Blumeria graminis, w którym dochodzi do amplifikacji określonego fragmentu DNA o długości 160 - 200 par zasad w reakcji PCR z zastosowaniem pary starterów oligonukleotydowych.The application relates to oligonucleotides enabling the presence of the genetic material of the fungal pathogen Blumeria graminis to be determined in the biological material tested using the polymerase chain reaction or its modification. The subject of the application is a pair of species-specific oligonucleotide primers for use in the polymerase chain reaction or its modification, with the sequences: LidBgl3: 5'-CGAGGTATCCGCAAYCATCA-3 'LidBgl4: 5'-GACGAGAACAGCACGAGGAA-3' complementary to species-specific genome sequence fragments. The subject of the application is also Blumeria graminis, a method of detecting the fungal pathogen of Blumeria graminis wheat, in which a specific fragment of DNA with a length of 160-200 base pairs is amplified in a PCR reaction using a pair of oligonucleotide primers.

PL419482A 2016-11-17 2016-11-17 Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it PL230741B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL419482A PL230741B1 (en) 2016-11-17 2016-11-17 Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL419482A PL230741B1 (en) 2016-11-17 2016-11-17 Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it

Publications (2)

Publication Number Publication Date
PL419482A1 true PL419482A1 (en) 2018-05-21
PL230741B1 PL230741B1 (en) 2018-12-31

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Family Applications (1)

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PL419482A PL230741B1 (en) 2016-11-17 2016-11-17 Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it

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PL (1) PL230741B1 (en)

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Publication number Publication date
PL230741B1 (en) 2018-12-31

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