PL419482A1 - Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it - Google Patents
Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting itInfo
- Publication number
- PL419482A1 PL419482A1 PL419482A PL41948216A PL419482A1 PL 419482 A1 PL419482 A1 PL 419482A1 PL 419482 A PL419482 A PL 419482A PL 41948216 A PL41948216 A PL 41948216A PL 419482 A1 PL419482 A1 PL 419482A1
- Authority
- PL
- Poland
- Prior art keywords
- detecting
- blumeria graminis
- oligonucleotide primers
- fungal pathogen
- application
- Prior art date
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Zgłoszenie dotyczy oligonukleotydów, umożliwiających stwierdzanie obecności materiału genetycznego patogenu grzybowego Blumeria graminis w badanym materiale biologicznym z wykorzystaniem łańcuchowej reakcji polimerazy lub jej modyfikacji. Przedmiot zgłoszenia stanowi para specyficznych gatunkowo starterów oligonukleotydowych do zastosowania w reakcji łańcuchowej polimerazy lub jej modyfikacji, o sekwencjach: LidBgl3: 5'-CGAGGTATCCGCAAYCATCA-3' LidBgl4: 5'-GACGAGAACAGCACGAGGAA-3' komplementarnych do specyficznych gatunkowo fragmentów sekwencji genomu. Przedmiotem zgłoszenia jest też Blumeria graminis sposób wykrywania patogenu grzybowego pszenicy Blumeria graminis, w którym dochodzi do amplifikacji określonego fragmentu DNA o długości 160 - 200 par zasad w reakcji PCR z zastosowaniem pary starterów oligonukleotydowych.The application relates to oligonucleotides enabling the presence of the genetic material of the fungal pathogen Blumeria graminis to be determined in the biological material tested using the polymerase chain reaction or its modification. The subject of the application is a pair of species-specific oligonucleotide primers for use in the polymerase chain reaction or its modification, with the sequences: LidBgl3: 5'-CGAGGTATCCGCAAYCATCA-3 'LidBgl4: 5'-GACGAGAACAGCACGAGGAA-3' complementary to species-specific genome sequence fragments. The subject of the application is also Blumeria graminis, a method of detecting the fungal pathogen of Blumeria graminis wheat, in which a specific fragment of DNA with a length of 160-200 base pairs is amplified in a PCR reaction using a pair of oligonucleotide primers.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PL419482A PL230741B1 (en) | 2016-11-17 | 2016-11-17 | Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PL419482A PL230741B1 (en) | 2016-11-17 | 2016-11-17 | Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it |
Publications (2)
Publication Number | Publication Date |
---|---|
PL419482A1 true PL419482A1 (en) | 2018-05-21 |
PL230741B1 PL230741B1 (en) | 2018-12-31 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PL419482A PL230741B1 (en) | 2016-11-17 | 2016-11-17 | Oligonucleotide primers for detecting wheat fungal pathogen Blumeria graminis and method for detecting it |
Country Status (1)
Country | Link |
---|---|
PL (1) | PL230741B1 (en) |
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2016
- 2016-11-17 PL PL419482A patent/PL230741B1/en unknown
Also Published As
Publication number | Publication date |
---|---|
PL230741B1 (en) | 2018-12-31 |
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