PL431170A1 - Oligonucleotide primers hybridizing within the Cyp51 gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection - Google Patents

Oligonucleotide primers hybridizing within the Cyp51 gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection

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Publication number
PL431170A1
PL431170A1 PL431170A PL43117019A PL431170A1 PL 431170 A1 PL431170 A1 PL 431170A1 PL 431170 A PL431170 A PL 431170A PL 43117019 A PL43117019 A PL 43117019A PL 431170 A1 PL431170 A1 PL 431170A1
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PL
Poland
Prior art keywords
oligonucleotide primers
detection
causing
detecting
fungal pathogen
Prior art date
Application number
PL431170A
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Polish (pl)
Other versions
PL238698B1 (en
Inventor
Adam Kuzdraliński
Justyna Leśniowska-Nowak
Michał Nowak
Magdalena Kawęcka
Karolina Różaniecka
Anna Kot
Agnieszka Ostrowska
Marta Muszyńska
Hubert Szczerba
Adam WAŚKO
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Uniwersytet Przyrodniczy W Lublinie
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Application filed by Uniwersytet Przyrodniczy W Lublinie filed Critical Uniwersytet Przyrodniczy W Lublinie
Priority to PL431170A priority Critical patent/PL238698B1/en
Publication of PL431170A1 publication Critical patent/PL431170A1/en
Publication of PL238698B1 publication Critical patent/PL238698B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiotem zgłoszenia są startery oligonukleotydowe hybrydyzujące w obrębie genu Cyp51 do wykrywania patogenu grzybowego pszenicy Zymoseptoria tritici o sekwencjach: LidSt11 5'-TTCGCGGACCTCTACCACTA-3' LidSt12 5'-TGGCATTGCCGTCCTTGTAT-3' Zgłoszenie obejmuje też sposób wykrywania patogenu grzybowego pszenicy Zymoseptoria tritici, w którym w reakcji PCR z zastosowaniem pary starterów dochodzi do amplifikacji określonego fragmentu DNA, po czym dokonuje się detekcji powstałego produktu, charakteryzujący się tym, że parę starterów stanowią startery oligonukleotydowe.The subject of the application are oligonucleotide primers hybridizing within the Cyp51 gene for the detection of the wheat fungal pathogen Zymoseptoria tritici with the following sequences: LidSt11 5'-TTCGCGGACCTCTACCACTA-3 'LidSt12 5'-TGGCATTGCCGTCCTTGTAT-3' wheat tritici PCR reaction with the use of a pair of primers, a specific fragment of DNA is amplified, and the resulting product is detected, characterized in that the pair of primers are oligonucleotide primers.

PL431170A 2019-09-16 2019-09-16 Oligonucleotide primers hybridizing within the Cyp51 gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection PL238698B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL431170A PL238698B1 (en) 2019-09-16 2019-09-16 Oligonucleotide primers hybridizing within the Cyp51 gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL431170A PL238698B1 (en) 2019-09-16 2019-09-16 Oligonucleotide primers hybridizing within the Cyp51 gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection

Publications (2)

Publication Number Publication Date
PL431170A1 true PL431170A1 (en) 2021-03-22
PL238698B1 PL238698B1 (en) 2021-09-27

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Family Applications (1)

Application Number Title Priority Date Filing Date
PL431170A PL238698B1 (en) 2019-09-16 2019-09-16 Oligonucleotide primers hybridizing within the Cyp51 gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection

Country Status (1)

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PL (1) PL238698B1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112961937A (en) * 2021-04-25 2021-06-15 兰州大学 Primer for detecting apocynum venetum septoria, kit and detection method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112961937A (en) * 2021-04-25 2021-06-15 兰州大学 Primer for detecting apocynum venetum septoria, kit and detection method thereof

Also Published As

Publication number Publication date
PL238698B1 (en) 2021-09-27

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