PL431159A1 - Oligonucleotide primers hybridizing within the Rpb2 gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection - Google Patents

Oligonucleotide primers hybridizing within the Rpb2 gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection

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Publication number
PL431159A1
PL431159A1 PL431159A PL43115919A PL431159A1 PL 431159 A1 PL431159 A1 PL 431159A1 PL 431159 A PL431159 A PL 431159A PL 43115919 A PL43115919 A PL 43115919A PL 431159 A1 PL431159 A1 PL 431159A1
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PL
Poland
Prior art keywords
detection
oligonucleotide primers
detecting
fungal pathogen
causing
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PL431159A
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Polish (pl)
Other versions
PL238696B1 (en
Inventor
Adam Kuzdraliński
Justyna Leśniowska-Nowak
Michał Nowak
Magdalena Kawęcka
Karolina Różaniecka
Anna Kot
Agnieszka Ostrowska
Marta Muszyńska
Hubert Szczerba
Adam WAŚKO
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Uniwersytet Przyrodniczy W Lublinie
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Application filed by Uniwersytet Przyrodniczy W Lublinie filed Critical Uniwersytet Przyrodniczy W Lublinie
Priority to PL431159A priority Critical patent/PL238696B1/en
Publication of PL431159A1 publication Critical patent/PL431159A1/en
Publication of PL238696B1 publication Critical patent/PL238696B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiotem zgłoszenia są startery oligonukleotydowe hybrydyzujące w obrębie genu Rpb2 do wykrywania patogenu grzybowego pszenicy Zymoseptoria tritici o sekwencjach: LidSt19 5'-GGTGAAGGACATGAGGCAGT-3' LidSt20 5'-CTTGCGGCCTTCTTTTGGTC-3'. Zgłoszenie, obejmuje także sposób wykrywania patogenu grzybowego pszenicy Zymoseptoria tritici, w którym w reakcji PCR z zastosowaniem pary starterów dochodzi do amplifikacji określonego fragmentu DNA, po czym dokonuje się detekcji powstałego produktu, znamienny tym, że parę starterów stanowią startery oligonukleotydowe wg zastrz.1.The subject of the application are oligonucleotide primers hybridizing within the Rpb2 gene for the detection of the wheat fungal pathogen Zymoseptoria tritici with the sequences: LidSt19 5'-GGTGAAGGACATGAGGCAGT-3 'LidSt20 5'-CTTGCGGCCTTCTTTTGGTC-3'. The application also includes a method for detecting the wheat fungal pathogen Zymoseptoria tritici, in which a specific DNA fragment is amplified by PCR using a pair of primers, followed by detection of the resulting product, characterized in that the primer pair are oligonucleotide primers according to claim 1.

PL431159A 2019-09-16 2019-09-16 Oligonucleotide primers hybridizing within the Rpb2 gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection PL238696B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL431159A PL238696B1 (en) 2019-09-16 2019-09-16 Oligonucleotide primers hybridizing within the Rpb2 gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL431159A PL238696B1 (en) 2019-09-16 2019-09-16 Oligonucleotide primers hybridizing within the Rpb2 gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection

Publications (2)

Publication Number Publication Date
PL431159A1 true PL431159A1 (en) 2021-03-22
PL238696B1 PL238696B1 (en) 2021-09-27

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Family Applications (1)

Application Number Title Priority Date Filing Date
PL431159A PL238696B1 (en) 2019-09-16 2019-09-16 Oligonucleotide primers hybridizing within the Rpb2 gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection

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PL (1) PL238696B1 (en)

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Publication number Publication date
PL238696B1 (en) 2021-09-27

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