PL431159A1 - Oligonucleotide primers hybridizing within the Rpb2 gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection - Google Patents

Oligonucleotide primers hybridizing within the Rpb2 gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection

Info

Publication number
PL431159A1
PL431159A1 PL431159A PL43115919A PL431159A1 PL 431159 A1 PL431159 A1 PL 431159A1 PL 431159 A PL431159 A PL 431159A PL 43115919 A PL43115919 A PL 43115919A PL 431159 A1 PL431159 A1 PL 431159A1
Authority
PL
Poland
Prior art keywords
detection
oligonucleotide primers
detecting
fungal pathogen
causing
Prior art date
Application number
PL431159A
Other languages
Polish (pl)
Other versions
PL238696B1 (en
Inventor
Adam Kuzdraliński
Justyna Leśniowska-Nowak
Michał Nowak
Magdalena Kawęcka
Karolina Różaniecka
Anna Kot
Agnieszka Ostrowska
Marta Muszyńska
Hubert Szczerba
Adam WAŚKO
Original Assignee
Uniwersytet Przyrodniczy W Lublinie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Przyrodniczy W Lublinie filed Critical Uniwersytet Przyrodniczy W Lublinie
Priority to PL431159A priority Critical patent/PL238696B1/en
Publication of PL431159A1 publication Critical patent/PL431159A1/en
Publication of PL238696B1 publication Critical patent/PL238696B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiotem zgłoszenia są startery oligonukleotydowe hybrydyzujące w obrębie genu Rpb2 do wykrywania patogenu grzybowego pszenicy Zymoseptoria tritici o sekwencjach: LidSt19 5'-GGTGAAGGACATGAGGCAGT-3' LidSt20 5'-CTTGCGGCCTTCTTTTGGTC-3'. Zgłoszenie, obejmuje także sposób wykrywania patogenu grzybowego pszenicy Zymoseptoria tritici, w którym w reakcji PCR z zastosowaniem pary starterów dochodzi do amplifikacji określonego fragmentu DNA, po czym dokonuje się detekcji powstałego produktu, znamienny tym, że parę starterów stanowią startery oligonukleotydowe wg zastrz.1.The subject of the application are oligonucleotide primers hybridizing within the Rpb2 gene for the detection of the wheat fungal pathogen Zymoseptoria tritici with the sequences: LidSt19 5'-GGTGAAGGACATGAGGCAGT-3 'LidSt20 5'-CTTGCGGCCTTCTTTTGGTC-3'. The application also includes a method for detecting the wheat fungal pathogen Zymoseptoria tritici, in which a specific DNA fragment is amplified by PCR using a pair of primers, followed by detection of the resulting product, characterized in that the primer pair are oligonucleotide primers according to claim 1.

PL431159A 2019-09-16 2019-09-16 Oligonucleotide primers hybridizing within the Rpb2 gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection PL238696B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL431159A PL238696B1 (en) 2019-09-16 2019-09-16 Oligonucleotide primers hybridizing within the Rpb2 gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL431159A PL238696B1 (en) 2019-09-16 2019-09-16 Oligonucleotide primers hybridizing within the Rpb2 gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection

Publications (2)

Publication Number Publication Date
PL431159A1 true PL431159A1 (en) 2021-03-22
PL238696B1 PL238696B1 (en) 2021-09-27

Family

ID=75107931

Family Applications (1)

Application Number Title Priority Date Filing Date
PL431159A PL238696B1 (en) 2019-09-16 2019-09-16 Oligonucleotide primers hybridizing within the Rpb2 gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection

Country Status (1)

Country Link
PL (1) PL238696B1 (en)

Also Published As

Publication number Publication date
PL238696B1 (en) 2021-09-27

Similar Documents

Publication Publication Date Title
SA516371691B1 (en) Methods and compositions for dna profiling
CY1121733T1 (en) RNAI-INDUCED REPRESSION OF THE HUNTINGTIN GENE
WO2015123430A3 (en) Single molecule electronic multiplex snp assay and pcr analysis
SG10201901475VA (en) Biochemically activated electronic device
SG10201808952WA (en) Systems and methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications
SG10201804076RA (en) Methods for multiplex detection of alleles associated with ophthalmic conditions
EP4293125A3 (en) Methods for targeted genomic analysis
MX2021000946A (en) Nucleic acid detection method.
MY195854A (en) Primer Set for Pcr for Bacterial Dna Amplification, Kit For Detection and/or Identification of Bacterial Species, and, Method of Detection and/or Identification of Bacterial Species
SG10201802281XA (en) Assay For The Diagnosis Of Dermatophytosis
MX2017000391A (en) Dna amplification technology.
DE602008006254D1 (en) DOUBLE-OLIGONUCLEOTIDE NUCLEIC DETECTION METHOD
EP3822349A4 (en) Dna polymerase mutant suited to nucleic acid amplification from rna
NZ598008A (en) An assay for determining a molecular risk assessment of a complex polymicrobial sample suspected to contain an ehec
EA201491821A1 (en) MODIFIED oligonucleotides, including thiol functional groups, and their use for the detection of nucleic acids
PL431170A1 (en) Oligonucleotide primers hybridizing within the Cyp51 gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection
WO2018194437A3 (en) Nucleic acid molecule template for detecting target point mutation gene and genetic analysis method using same
PL431159A1 (en) Oligonucleotide primers hybridizing within the Rpb2 gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection
PL431160A1 (en) Oligonucleotide primers hybridizing within the SdhB gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection
WO2018049315A8 (en) Acrylamide copolymerization for sequestration and production of single-stranded nucleic acid
ES2855202T3 (en) Prediction of genetic resistance against antimicrobial drugs in microorganisms using structural changes in the genome
BR112019009488A2 (en) electrochemical dna detection
PL419487A1 (en) Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting it
PL417583A1 (en) Pair of starter oligonucleotides for molecular detection of the dwarfism gene dw8 presence in rye plants and method for molecular detection of the dwarfism gene dw8 presence in rye plants
PL419486A1 (en) Oligonucleotide primers for detecting wheat fungal pathogen Puccinia recondita and method for detecting it