PL431160A1 - Oligonucleotide primers hybridizing within the SdhB gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection - Google Patents

Oligonucleotide primers hybridizing within the SdhB gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection

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Publication number
PL431160A1
PL431160A1 PL431160A PL43116019A PL431160A1 PL 431160 A1 PL431160 A1 PL 431160A1 PL 431160 A PL431160 A PL 431160A PL 43116019 A PL43116019 A PL 43116019A PL 431160 A1 PL431160 A1 PL 431160A1
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PL
Poland
Prior art keywords
detection
oligonucleotide primers
detecting
fungal pathogen
causing
Prior art date
Application number
PL431160A
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Polish (pl)
Other versions
PL238697B1 (en
Inventor
Adam Kuzdraliński
Justyna Leśniowska-Nowak
Michał Nowak
Magdalena Kawęcka
Karolina Różaniecka
Anna Kot
Agnieszka Ostrowska
Marta Muszyńska
Hubert Szczerba
Adam WAŚKO
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Uniwersytet Przyrodniczy W Lublinie
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Application filed by Uniwersytet Przyrodniczy W Lublinie filed Critical Uniwersytet Przyrodniczy W Lublinie
Priority to PL431160A priority Critical patent/PL238697B1/en
Publication of PL431160A1 publication Critical patent/PL431160A1/en
Publication of PL238697B1 publication Critical patent/PL238697B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiotem zgłoszenia są startery oligonukleotydowe hybrydyzujące w obrębie genu SdhB do wykrywania patogenu grzybowego pszenicy Zymoseptoria tritici o sekwencjach: LidSt15 5'-TGCACCATCCTTCACCTTCC-3' LidSt16 5'-CGAGTCGTTGATCCATCGGT-3'. Zgłoszenie obejmuje też sposób wykrywania patogenu grzybowego pszenicy Zymoseptoria tritici, w którym w reakcji PCR z zastosowaniem pary starterów dochodzi do amplifikacji określonego fragmentu DNA, po czym dokonuje się detekcji powstałego produktu, charakteryzujący się tym, że parę starterów stanowią startery oligonukleotydowe.The subject of the application are oligonucleotide primers hybridizing within the SdhB gene for the detection of the wheat fungal pathogen Zymoseptoria tritici with the sequences: LidSt15 5'-TGCACCATCCTTCACCTTCC-3 'LidSt16 5'-CGAGTCGTTGATCCATCGGT-3'. The application also covers a method for detecting the wheat fungal pathogen Zymoseptoria tritici, in which a specific DNA fragment is amplified by PCR using a pair of primers, followed by detection of the resulting product, characterized in that the primer pair is oligonucleotide primers.

PL431160A 2019-09-16 2019-09-16 Oligonucleotide primers hybridizing within the SdhB gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection PL238697B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL431160A PL238697B1 (en) 2019-09-16 2019-09-16 Oligonucleotide primers hybridizing within the SdhB gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL431160A PL238697B1 (en) 2019-09-16 2019-09-16 Oligonucleotide primers hybridizing within the SdhB gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection

Publications (2)

Publication Number Publication Date
PL431160A1 true PL431160A1 (en) 2021-03-22
PL238697B1 PL238697B1 (en) 2021-09-27

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ID=75107933

Family Applications (1)

Application Number Title Priority Date Filing Date
PL431160A PL238697B1 (en) 2019-09-16 2019-09-16 Oligonucleotide primers hybridizing within the SdhB gene for detecting the wheat fungal pathogen Zymoseptoria tritici causing septoria leaf blotch, and method of its detection

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PL (1) PL238697B1 (en)

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Publication number Publication date
PL238697B1 (en) 2021-09-27

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