PH25983A - DNA fragments containing alkaline cellulase gene recombinant plasmids with said DNA fragments inserted therein and recombinant microorganism - Google Patents
DNA fragments containing alkaline cellulase gene recombinant plasmids with said DNA fragments inserted therein and recombinant microorganism Download PDFInfo
- Publication number
- PH25983A PH25983A PH35995A PH35995A PH25983A PH 25983 A PH25983 A PH 25983A PH 35995 A PH35995 A PH 35995A PH 35995 A PH35995 A PH 35995A PH 25983 A PH25983 A PH 25983A
- Authority
- PH
- Philippines
- Prior art keywords
- dna fragment
- alkaline
- base pairs
- cellulase
- alkaline cellulase
- Prior art date
Links
- 239000012634 fragment Substances 0.000 title claims description 84
- 239000013612 plasmid Substances 0.000 title claims description 52
- 101710166469 Endoglucanase Proteins 0.000 title claims description 37
- 244000005700 microbiome Species 0.000 title claims description 12
- 238000000034 method Methods 0.000 description 38
- 108090000623 proteins and genes Proteins 0.000 description 30
- 108010059892 Cellulase Proteins 0.000 description 28
- 239000013598 vector Substances 0.000 description 25
- 238000003776 cleavage reaction Methods 0.000 description 23
- 230000007017 scission Effects 0.000 description 23
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 20
- 229940106157 cellulase Drugs 0.000 description 17
- 102000004190 Enzymes Human genes 0.000 description 16
- 108090000790 Enzymes Proteins 0.000 description 16
- 229940088598 enzyme Drugs 0.000 description 16
- 230000000694 effects Effects 0.000 description 14
- 108010085318 carboxymethylcellulase Proteins 0.000 description 13
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 12
- 239000002609 medium Substances 0.000 description 11
- 238000006243 chemical reaction Methods 0.000 description 10
- 239000013611 chromosomal DNA Substances 0.000 description 10
- 230000014509 gene expression Effects 0.000 description 10
- 239000011780 sodium chloride Substances 0.000 description 10
- 241000193830 Bacillus <bacterium> Species 0.000 description 9
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 8
- 230000001580 bacterial effect Effects 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 7
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 7
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 6
- 238000000246 agarose gel electrophoresis Methods 0.000 description 6
- 239000000284 extract Substances 0.000 description 6
- 108091008146 restriction endonucleases Proteins 0.000 description 6
- 241000894007 species Species 0.000 description 6
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 5
- 239000002253 acid Substances 0.000 description 5
- 229960000723 ampicillin Drugs 0.000 description 5
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 5
- 239000000872 buffer Substances 0.000 description 5
- IQFVPQOLBLOTPF-HKXUKFGYSA-L congo red Chemical compound [Na+].[Na+].C1=CC=CC2=C(N)C(/N=N/C3=CC=C(C=C3)C3=CC=C(C=C3)/N=N/C3=C(C4=CC=CC=C4C(=C3)S([O-])(=O)=O)N)=CC(S([O-])(=O)=O)=C21 IQFVPQOLBLOTPF-HKXUKFGYSA-L 0.000 description 5
- 238000005304 joining Methods 0.000 description 5
- 239000002244 precipitate Substances 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- 244000063299 Bacillus subtilis Species 0.000 description 4
- 235000014469 Bacillus subtilis Nutrition 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- 101150042441 K gene Proteins 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 4
- 229940041514 candida albicans extract Drugs 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 238000004587 chromatography analysis Methods 0.000 description 4
- 239000003599 detergent Substances 0.000 description 4
- -1 fatty acid ester sodium salt Chemical class 0.000 description 4
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 230000007935 neutral effect Effects 0.000 description 4
- 238000001556 precipitation Methods 0.000 description 4
- 159000000000 sodium salts Chemical class 0.000 description 4
- 239000012138 yeast extract Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- 102000012410 DNA Ligases Human genes 0.000 description 3
- 108010061982 DNA Ligases Proteins 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 239000006142 Luria-Bertani Agar Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 108010091371 endoglucanase 1 Proteins 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 235000013372 meat Nutrition 0.000 description 3
- 238000010369 molecular cloning Methods 0.000 description 3
- 238000007747 plating Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000012429 reaction media Substances 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- LWFUFLREGJMOIZ-UHFFFAOYSA-N 3,5-dinitrosalicylic acid Chemical compound OC(=O)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1O LWFUFLREGJMOIZ-UHFFFAOYSA-N 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- 241000588722 Escherichia Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- 101710163270 Nuclease Proteins 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- 239000004098 Tetracycline Substances 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 239000001768 carboxy methyl cellulose Substances 0.000 description 2
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 2
- 230000000875 corresponding effect Effects 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 229920001519 homopolymer Polymers 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000013049 sediment Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- 229960002180 tetracycline Drugs 0.000 description 2
- 229930101283 tetracycline Natural products 0.000 description 2
- 235000019364 tetracycline Nutrition 0.000 description 2
- 239000012137 tryptone Substances 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- IAYJZWFYUSNIPN-KFRZSCGFSA-N (2s,3r,4s,5s,6r)-2-[(2r,3s,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-(4-nitrophenoxy)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](OC=2C=CC(=CC=2)[N+]([O-])=O)[C@H](O)[C@H]1O IAYJZWFYUSNIPN-KFRZSCGFSA-N 0.000 description 1
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
- 241001019659 Acremonium <Plectosphaerellaceae> Species 0.000 description 1
- 101100238763 Bacillus subtilis hsdRM gene Proteins 0.000 description 1
- 101100497221 Bacillus thuringiensis subsp. alesti cry1Ae gene Proteins 0.000 description 1
- 108010023063 Bacto-peptone Proteins 0.000 description 1
- 241000186321 Cellulomonas Species 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 1
- 101150105088 Dele1 gene Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 101710156496 Endoglucanase A Proteins 0.000 description 1
- 108010056771 Glucosidases Proteins 0.000 description 1
- 102000004366 Glucosidases Human genes 0.000 description 1
- 241000223198 Humicola Species 0.000 description 1
- 241001214257 Mene Species 0.000 description 1
- 244000202563 Meum athamanticum Species 0.000 description 1
- 101100114416 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) con-10 gene Proteins 0.000 description 1
- 101150030170 Paat gene Proteins 0.000 description 1
- 241000791420 Plica Species 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- 101710194948 Protein phosphatase PhpP Proteins 0.000 description 1
- 101000906479 Saitozyma flava Endoglucanase 1 Proteins 0.000 description 1
- 241000219289 Silene Species 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 101100020289 Xenopus laevis koza gene Proteins 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- FHKPLLOSJHHKNU-INIZCTEOSA-N [(3S)-3-[8-(1-ethyl-5-methylpyrazol-4-yl)-9-methylpurin-6-yl]oxypyrrolidin-1-yl]-(oxan-4-yl)methanone Chemical compound C(C)N1N=CC(=C1C)C=1N(C2=NC=NC(=C2N=1)O[C@@H]1CN(CC1)C(=O)C1CCOCC1)C FHKPLLOSJHHKNU-INIZCTEOSA-N 0.000 description 1
- ZVQOOHYFBIDMTQ-UHFFFAOYSA-N [methyl(oxido){1-[6-(trifluoromethyl)pyridin-3-yl]ethyl}-lambda(6)-sulfanylidene]cyanamide Chemical compound N#CN=S(C)(=O)C(C)C1=CC=C(C(F)(F)F)N=C1 ZVQOOHYFBIDMTQ-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 150000005215 alkyl ethers Chemical group 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229910052586 apatite Inorganic materials 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 108010047754 beta-Glucosidase Proteins 0.000 description 1
- 102000006995 beta-Glucosidase Human genes 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910052792 caesium Inorganic materials 0.000 description 1
- TVFDJXOCXUVLDH-UHFFFAOYSA-N caesium atom Chemical compound [Cs] TVFDJXOCXUVLDH-UHFFFAOYSA-N 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- FYGDTMLNYKFZSV-ZWSAEMDYSA-N cellotriose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](OC(O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-ZWSAEMDYSA-N 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 230000005757 colony formation Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000012262 fermentative production Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 238000005227 gel permeation chromatography Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 101150102883 hsdM gene Proteins 0.000 description 1
- 101150085823 hsdR gene Proteins 0.000 description 1
- 230000000951 immunodiffusion Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000007169 ligase reaction Methods 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 235000015250 liver sausages Nutrition 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000010445 mica Substances 0.000 description 1
- 229910052618 mica group Inorganic materials 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- HWGNBUXHKFFFIH-UHFFFAOYSA-I pentasodium;[oxido(phosphonatooxy)phosphoryl] phosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O HWGNBUXHKFFFIH-UHFFFAOYSA-I 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000013492 plasmid preparation Methods 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 239000001044 red dye Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000019832 sodium triphosphate Nutrition 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- OFVLGDICTFRJMM-WESIUVDSSA-N tetracycline Chemical compound C1=CC=C2[C@](O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O OFVLGDICTFRJMM-WESIUVDSSA-N 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP25992386 | 1986-10-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PH25983A true PH25983A (en) | 1992-01-13 |
Family
ID=17340805
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PH35995A PH25983A (en) | 1986-10-31 | 1987-10-28 | DNA fragments containing alkaline cellulase gene recombinant plasmids with said DNA fragments inserted therein and recombinant microorganism |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US4904599A (de) |
| EP (1) | EP0273125B1 (de) |
| JP (1) | JP2530181B2 (de) |
| DE (1) | DE3785164T2 (de) |
| DK (1) | DK568587A (de) |
| ES (1) | ES2054642T3 (de) |
| HK (1) | HK37994A (de) |
| MY (1) | MY102992A (de) |
| PH (1) | PH25983A (de) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5298405A (en) * | 1986-04-30 | 1994-03-29 | Alko Limited | Enzyme preparations with recombinantly-altered cellulose profiles and methods for their production |
| US4945053A (en) * | 1986-10-28 | 1990-07-31 | Kao Corporation | Novel alkaline cellulases and a microorganism for producing the same |
| JP2652871B2 (ja) * | 1988-04-25 | 1997-09-10 | 花王株式会社 | アルカリセルラーゼおよびその製造法 |
| US5169554A (en) * | 1989-10-04 | 1992-12-08 | The United States Of America As Represented By The Secretary Of The Army | Enzyme detergent formulation and methods of detoxifying toxic organophosphorous acid compounds |
| US5120463A (en) * | 1989-10-19 | 1992-06-09 | Genencor International, Inc. | Degradation resistant detergent compositions based on cellulase enzymes |
| US5475101A (en) * | 1990-10-05 | 1995-12-12 | Genencor International, Inc. | DNA sequence encoding endoglucanase III cellulase |
| JPH07506404A (ja) * | 1992-05-01 | 1995-07-13 | ジェネンコア インターナショナル インコーポレーテッド | 綿含有織物をｃｂｈ ｉが多いセルラーゼで処理する方法 |
| US5945327A (en) * | 1992-07-02 | 1999-08-31 | Novo Nordisk A/S | DNA constructs and methods of producing cellulytic enzymes |
| US5514584A (en) * | 1993-06-11 | 1996-05-07 | Midwest Research Institute | Cloning of cellulase genes from acidothermus cellulolyticus |
| US5691295A (en) * | 1995-01-17 | 1997-11-25 | Cognis Gesellschaft Fuer Biotechnologie Mbh | Detergent compositions |
| DE4422433A1 (de) * | 1994-06-28 | 1996-01-04 | Cognis Bio Umwelt | Mehrenzymgranulat |
| US5646044A (en) * | 1995-03-02 | 1997-07-08 | Henkel Kommanditgesellschaft Auf Aktien | Expression systems for the production of target proteins in bacillus |
| ES2242234T3 (es) * | 1996-09-12 | 2005-11-01 | Syngenta Participations Ag | Plantas transgenicas que expresan enzimas celuloliticas. |
| DE19725508A1 (de) | 1997-06-17 | 1998-12-24 | Clariant Gmbh | Wasch- und Reinigungsmittel |
| DE19914811A1 (de) | 1999-03-31 | 2000-10-05 | Henkel Kgaa | Enzym- und bleichaktivatorhaltige Wasch- und Reinigungsmittel |
| DE60238339D1 (de) | 2001-08-27 | 2010-12-30 | Syngenta Participations Ag | Selbstprozessierende pflanzen und pflanzenteile |
| EP1438346A1 (de) | 2001-10-22 | 2004-07-21 | Henkel Kommanditgesellschaft auf Aktien | Baumwollaktive schmutzablösevermögende polymere auf urethan-basis |
| ATE357497T1 (de) | 2002-12-20 | 2007-04-15 | Henkel Kgaa | Bleichmittelhaltige wasch- oder reinigungsmittel |
| WO2004097001A2 (en) | 2003-04-29 | 2004-11-11 | Genencor International, Inc. | Novel bacillus 029cel cellulase |
| EP1730284A4 (de) * | 2004-03-08 | 2008-04-30 | Syngenta Participations Ag | Selbstprozessierende pflanzen und pflanzenteile |
| US8524474B2 (en) * | 2008-11-20 | 2013-09-03 | E I Du Pont De Nemours And Company | Process for producing a concentrated sugar solution by enzymatic saccharification of polysaccharide enriched biomass |
| WO2010059796A2 (en) * | 2008-11-20 | 2010-05-27 | E. I. Du Pont De Nemours And Company | Process for producing a sugar solution by combined chemical and enzymatic saccharification of polysaccharide enriched biomass |
-
1987
- 1987-10-16 JP JP62261488A patent/JP2530181B2/ja not_active Expired - Fee Related
- 1987-10-19 US US07/109,510 patent/US4904599A/en not_active Expired - Lifetime
- 1987-10-21 ES ES87115431T patent/ES2054642T3/es not_active Expired - Lifetime
- 1987-10-21 EP EP87115431A patent/EP0273125B1/de not_active Expired - Lifetime
- 1987-10-21 DE DE87115431T patent/DE3785164T2/de not_active Expired - Fee Related
- 1987-10-28 PH PH35995A patent/PH25983A/en unknown
- 1987-10-30 DK DK568587A patent/DK568587A/da not_active Application Discontinuation
- 1987-10-31 MY MYPI87003002A patent/MY102992A/en unknown
-
1994
- 1994-04-21 HK HK37994A patent/HK37994A/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| JP2530181B2 (ja) | 1996-09-04 |
| DK568587A (da) | 1988-05-01 |
| EP0273125A2 (de) | 1988-07-06 |
| JPS63233792A (ja) | 1988-09-29 |
| EP0273125B1 (de) | 1993-03-31 |
| ES2054642T3 (es) | 1994-08-16 |
| EP0273125A3 (en) | 1988-11-17 |
| DE3785164T2 (de) | 1993-11-04 |
| US4904599A (en) | 1990-02-27 |
| DK568587D0 (da) | 1987-10-30 |
| MY102992A (en) | 1993-03-31 |
| HK37994A (en) | 1994-04-29 |
| DE3785164D1 (de) | 1993-05-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| PH25983A (en) | DNA fragments containing alkaline cellulase gene recombinant plasmids with said DNA fragments inserted therein and recombinant microorganism | |
| Reinhold-Hurek et al. | Cloning, expression in Escherichia coli, and characterization of cellulolytic enzymes of Azoarcus sp., a root-invading diazotroph | |
| Saul et al. | celB, a gene coding for a bifunctional cellulase from the extreme thermophile" Caldocellum saccharolyticum" | |
| Cornet et al. | Characterization of two cel (cellulose degradation) genes of Clostridium thermocellum coding for endoglucanases | |
| EP0494207B1 (de) | Hitzestabile gereinigte cellulase aus dem thermophilen bakterium acidothermus cellulolyticus | |
| Tsujibo et al. | Purification, properties, and partial amino acid sequences of thermostable xylanases from Streptomyces thermoviolaceus OPC-520 | |
| Hakamada et al. | Thermostable alkaline cellulase from an alkaliphilic isolate, Bacillus sp. KSM-S237 | |
| JP2652871B2 (ja) | アルカリセルラーゼおよびその製造法 | |
| GB2133408A (en) | Method for improving the production of proteins in bacillus | |
| Barros et al. | Cloning and expression in Escherichia coli of a cellulase gene from Ruminococcus flavefaciens | |
| EP0176971A2 (de) | Für ein thermostabiles Beta-Galaktosidase, kodierendes Gen, dieses Gen enthaltender Bacillus-subtilis, das durch dieses Gen kodiertes Enzym und Verfahren zu dessen Herstellung | |
| Hansen et al. | celA from Bacillus lautus PL236 encodes a novel cellulose-binding endo-beta-1, 4-glucanase | |
| Sami et al. | Production of free and substrate-bound cellulases of Cellulomonas flavigena | |
| FI95481C (fi) | Alkalinen pullulanaasi, sitä tuottava mikro-organismi ja menetelmä sen tuottamiseksi | |
| Huang et al. | Molecular cloning and expression of multiple cellulase genes of Ruminococcus flavefaciens strain 186 in Escherichia coli | |
| Dijkerman et al. | The role of the cellulolytic high molecular mass (HMM) complex of the anaerobic fungus Piromyces sp. strain E2 in the hydrolysis of microcrystalline cellulose | |
| Gokhale et al. | Differential expression of xylanases and endoglucanases in the hybrid derived from intergeneric protoplast fusion between a Cellulomonas sp. and Bacillus subtilis | |
| Kim et al. | Molecular cloning of an endoglucanase gene from an alkalophilic Bacillus sp. and its expression in Escherichia coli | |
| Ganju et al. | Purification and characterization of two cellobiohydrolases from Chaetomium thermophile var. coprophile | |
| EP0838521A1 (de) | Carboxymethylcellulase und Mikroorganismen die sie produzieren | |
| KR100341451B1 (ko) | 루미노코쿠스알부스유래의베타-글루코시다제유전자 | |
| Honda et al. | Production of extracellular alkaline xylanase of alkalophilic Bacillus sp. C-125 by Escherichia coli carrying pCX 311 | |
| Imam et al. | Zymographic analyses of carboxymethylcellulases secreted by the bacterium from wood-boring marine shipworms | |
| JP2879682B2 (ja) | バンコマイシンまたはリストセチン耐性変異株及びその製法 | |
| JP2592289B2 (ja) | アルカリセルラーゼ遺伝子を含有するｄｎａ断片並びに該ｄｎａ断片を組み込んだベクター及びその含有微生物 |