NZ617099A - Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof - Google Patents

Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof Download PDF

Info

Publication number
NZ617099A
NZ617099A NZ617099A NZ61709913A NZ617099A NZ 617099 A NZ617099 A NZ 617099A NZ 617099 A NZ617099 A NZ 617099A NZ 61709913 A NZ61709913 A NZ 61709913A NZ 617099 A NZ617099 A NZ 617099A
Authority
NZ
New Zealand
Prior art keywords
cells
cell
culture
aggregates
roller bottle
Prior art date
Application number
NZ617099A
Other languages
English (en)
Inventor
Thomas C Schulz
Original Assignee
Viacyte Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/672,688 external-priority patent/US8895300B2/en
Application filed by Viacyte Inc filed Critical Viacyte Inc
Priority to NZ702500A priority Critical patent/NZ702500A/en
Publication of NZ617099A publication Critical patent/NZ617099A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/10—Rotating vessel
    • C12M27/12—Roller bottles; Roller tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/195—Heregulin, neu differentiation factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Reproductive Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Transplantation (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Public Health (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Sustainable Development (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Analytical Chemistry (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Professional, Industrial, Or Sporting Protective Garments (AREA)
NZ617099A 2012-11-08 2013-10-29 Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof NZ617099A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
NZ702500A NZ702500A (en) 2012-11-08 2013-10-29 Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13/672,688 US8895300B2 (en) 2008-11-04 2012-11-08 Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof

Publications (1)

Publication Number Publication Date
NZ617099A true NZ617099A (en) 2015-05-29

Family

ID=49515254

Family Applications (2)

Application Number Title Priority Date Filing Date
NZ617099A NZ617099A (en) 2012-11-08 2013-10-29 Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
NZ702500A NZ702500A (en) 2012-11-08 2013-10-29 Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof

Family Applications After (1)

Application Number Title Priority Date Filing Date
NZ702500A NZ702500A (en) 2012-11-08 2013-10-29 Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof

Country Status (11)

Country Link
EP (2) EP2730649B1 (https=)
JP (5) JP6532098B2 (https=)
KR (6) KR102069900B1 (https=)
CN (2) CN108342354B (https=)
AU (5) AU2013248265B2 (https=)
CA (1) CA2832194A1 (https=)
DK (1) DK2730649T3 (https=)
ES (1) ES2906102T3 (https=)
IL (1) IL229137B (https=)
NZ (2) NZ617099A (https=)
SG (1) SG2013081450A (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9944894B2 (en) * 2015-01-16 2018-04-17 General Electric Company Pluripotent stem cell expansion and passage using a rocking platform bioreactor
WO2017127921A1 (en) * 2016-01-26 2017-08-03 Centre For Commercialization Of Regenerative Medicine Method for dissociating cell aggregates
CN111565707A (zh) 2017-06-14 2020-08-21 森玛治疗公司 用于递送治疗物的装置和方法
WO2019124348A1 (ja) * 2017-12-19 2019-06-27 国立大学法人京都大学 新規骨分化誘導方法
WO2020009188A1 (ja) * 2018-07-06 2020-01-09 株式会社マイオリッジ 細胞シートの製造方法、心筋細胞シート、および心筋細胞シート製造用キット
CN112469814A (zh) * 2018-07-27 2021-03-09 味之素株式会社 动物细胞的悬浮培养用添加物、悬浮培养用培养基和悬浮培养方法
EP4074819A4 (en) * 2019-12-09 2024-01-24 Daewoong Pharmaceutical Co., Ltd. Method for preparation of mesenchymal stem cell from human pluripotent stem cell and mesenchymal stem cells prepared thereby
JP7526460B2 (ja) * 2020-04-03 2024-08-01 国立研究開発法人農業・食品産業技術総合研究機構 試料冷却方法、冷却物の製造方法及び冷却用具
CN112705131B (zh) * 2020-12-29 2023-01-24 广西中烟工业有限责任公司 一种低共熔溶剂/羟丙基-β-环糊精薄荷醇微囊制备方法
US20220403340A1 (en) * 2021-06-11 2022-12-22 Washington University Media for cryopreserved cells and methods of making and using same
CN118600039B (zh) * 2024-06-24 2026-03-06 武汉光谷中源药业有限公司 一种检测iPSC残留的引物探针组合物及试剂盒与应用

Family Cites Families (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2161804A (en) 1935-11-09 1939-06-13 Baldwin Southwark Corp Hydraulic press
US4988623A (en) 1988-06-30 1991-01-29 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Rotating bio-reactor cell culture apparatus
US5605822A (en) 1989-06-15 1997-02-25 The Regents Of The University Of Michigan Methods, compositions and devices for growing human hematopoietic cells
US5437994A (en) 1989-06-15 1995-08-01 Regents Of The University Of Michigan Method for the ex vivo replication of stem cells, for the optimization of hematopoietic progenitor cell cultures, and for increasing the metabolism, GM-CSF secretion and/or IL-6 secretion of human stromal cells
EP0505148A1 (en) 1991-03-17 1992-09-23 Yeda Research And Development Company Limited Stimulating factor of the neu receptor
WO1993022424A1 (en) 1992-04-29 1993-11-11 Amgen Inc. RECOMBINANT STIMULATING FACTOR OF THE neu RECEPTOR
US5453357A (en) 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US5320963A (en) 1992-11-25 1994-06-14 National Research Council Of Canada Bioreactor for the perfusion culture of cells
WO1994028133A1 (en) 1993-05-21 1994-12-08 Amgen Inc. Recombinant neu differentiation factors
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
JP2001517927A (ja) 1996-02-28 2001-10-09 バンダービルト ユニバーシティ 胚幹細胞を作製する組成物および方法
US5866419A (en) 1996-09-16 1999-02-02 Meder; Martin G. Roller bottle
US6387638B1 (en) 1997-02-10 2002-05-14 Genentech, Inc. Heregulin variants
US6136558A (en) 1997-02-10 2000-10-24 Genentech, Inc. Heregulin variants
US6331406B1 (en) 1997-03-31 2001-12-18 The John Hopkins University School Of Medicine Human enbryonic germ cell and methods of use
CA2324591A1 (en) 1998-04-09 1999-10-21 Bresagen Limited Cell differentiation/proliferation and maintenance factor and uses thereof
US6667176B1 (en) * 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US6875607B1 (en) 1998-11-09 2005-04-05 Es Cell International Pte Ltd Embryonic stem cells
US7005252B1 (en) 2000-03-09 2006-02-28 Wisconsin Alumni Research Foundation Serum free cultivation of primate embryonic stem cells
US6416999B1 (en) * 2000-04-07 2002-07-09 Raven Biotechnologies, Inc. Human Müllerian duct-derived epithelial cells and methods of isolation and uses thereof
WO2003014313A2 (en) 2001-08-06 2003-02-20 Bresagen, Ltd. Alternative compositions and methods for the culture of stem cells
US20040161419A1 (en) * 2002-04-19 2004-08-19 Strom Stephen C. Placental stem cells and uses thereof
US20060030042A1 (en) 2003-12-19 2006-02-09 Ali Brivanlou Maintenance of embryonic stem cells by the GSK-3 inhibitor 6-bromoindirubin-3'-oxime
US7541185B2 (en) 2003-12-23 2009-06-02 Cythera, Inc. Methods for identifying factors for differentiating definitive endoderm
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
CN103898047B (zh) 2003-12-23 2020-03-03 维亚希特公司 定形内胚层
US7625753B2 (en) 2003-12-23 2009-12-01 Cythera, Inc. Expansion of definitive endoderm cells
WO2005065354A2 (en) 2003-12-31 2005-07-21 The Burnham Institute Defined media for pluripotent stem cell culture
DE102004043256B4 (de) * 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
US7739764B2 (en) 2005-04-27 2010-06-22 Whirlpool Corporation Method and apparatus for monitoring load size and load imbalance in washing machine
WO2007051038A2 (en) 2005-10-27 2007-05-03 Cythera, Inc. Pdx1-expressing dorsal and ventral foregut endoderm
SG170021A1 (en) 2006-02-23 2011-04-29 Novocell Inc Compositions and methods useful for culturing differentiable cells
US7695965B2 (en) 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
JP6110048B2 (ja) 2006-03-02 2017-04-05 ヴィアサイト，インコーポレイテッド 内分泌前駆細胞、膵臓ホルモン発現細胞及びそれらの製造方法
AU2007277364B2 (en) 2006-07-26 2010-08-12 Viacyte, Inc. Methods of producing pancreatic hormones
JP2008099662A (ja) * 2006-09-22 2008-05-01 Institute Of Physical & Chemical Research 幹細胞の培養方法
US9080145B2 (en) * 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
EP2185694A2 (en) * 2007-08-02 2010-05-19 California Stem Cell, Inc. Neuronal progenitor cells and methods of derivation and purification of neuronal progenitor cells from embryonic stem cells
US7695963B2 (en) 2007-09-24 2010-04-13 Cythera, Inc. Methods for increasing definitive endoderm production
US8623650B2 (en) * 2007-10-19 2014-01-07 Viacyte, Inc. Methods and compositions for feeder-free pluripotent stem cell media containing human serum
CN101599496B (zh) 2008-06-06 2011-06-15 群康科技(深圳)有限公司 薄膜晶体管基板与薄膜晶体管母基板
US8639996B2 (en) * 2008-07-11 2014-01-28 Qualcomm Incorporated Systems and methods for uplink inter-cell interference cancellation using hybrid automatic repeat request (HARQ) retransmissions
JP5390624B2 (ja) * 2008-11-04 2014-01-15 バイアサイト インク 幹細胞集合体懸濁液組成物、その分化方法
US8008075B2 (en) * 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
JP2012508584A (ja) 2008-11-14 2012-04-12 ヴィアサイト，インコーポレイテッド ヒト多能性幹細胞由来膵臓細胞のカプセル化
JP2012520672A (ja) * 2009-03-20 2012-09-10 エージェンシー フォー サイエンス，テクノロジー アンド リサーチ マイクロキャリアー上の多能性（ｐｌｕｒｉｐｏｔｅｎｔ）および多分化能（ｍｕｌｔｉｐｏｔｅｎｔ）細胞の培養
EP2421957B1 (en) 2009-04-22 2020-11-18 Viacyte, Inc. Cell compositions derived from dedifferentiated reprogrammed cells
DK2694644T3 (en) * 2011-03-30 2018-04-16 Cellular Dynamics Int Inc Priming of pluripotent stem cells for neural differentiation
US10702008B2 (en) 2018-02-26 2020-07-07 Hbn Shoe, Llc Device and method of constructing shoes
US11586805B2 (en) 2021-07-26 2023-02-21 Atlassian Pty Ltd. Machine-learning-based natural language processing techniques for low-latency document summarization

Also Published As

Publication number Publication date
JP2014094005A (ja) 2014-05-22
JP6767445B2 (ja) 2020-10-14
CN108342354A (zh) 2018-07-31
ES2906102T3 (es) 2022-04-13
JP2021007393A (ja) 2021-01-28
AU2013248265B2 (en) 2018-11-01
AU2022203343A1 (en) 2022-06-09
EP2730649A1 (en) 2014-05-14
JP2025028837A (ja) 2025-03-05
AU2019200532A1 (en) 2019-02-14
AU2022203343B2 (en) 2023-05-25
KR102684120B1 (ko) 2024-07-12
CN103805512A (zh) 2014-05-21
KR20240113877A (ko) 2024-07-23
KR102357281B1 (ko) 2022-02-07
KR20230022928A (ko) 2023-02-16
KR20140059729A (ko) 2014-05-16
JP2019022496A (ja) 2019-02-14
EP4053264A1 (en) 2022-09-07
JP7580508B2 (ja) 2024-11-11
JP6532098B2 (ja) 2019-06-19
KR20200008172A (ko) 2020-01-23
AU2013248265A1 (en) 2014-05-22
NZ702500A (en) 2016-07-29
CN108342354B (zh) 2022-03-04
EP2730649B1 (en) 2021-12-22
AU2019200532B2 (en) 2022-02-24
CA2832194A1 (en) 2014-05-08
KR20200130798A (ko) 2020-11-20
JP2023075135A (ja) 2023-05-30
AU2026200342A1 (en) 2026-02-05
KR102180779B1 (ko) 2020-11-19
AU2023216861B2 (en) 2025-10-23
SG2013081450A (en) 2014-06-27
CN103805512B (zh) 2018-03-27
KR102069900B1 (ko) 2020-02-11
IL229137A0 (en) 2014-03-31
AU2023216861A1 (en) 2023-09-07
KR102498731B1 (ko) 2023-02-13
IL229137B (en) 2018-08-30
DK2730649T3 (da) 2022-03-07
KR20220018522A (ko) 2022-02-15

Similar Documents

Publication Publication Date Title
US12234477B2 (en) Scalable primate pluripotent stem cell aggregate suspension culture
AU2022203343B2 (en) Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
EP2356213B1 (en) Stem cell aggregate suspension compositions and methods for differentiation thereof
HK40080876A (en) Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
HK1191376A (en) Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof

Legal Events

Date Code Title Description
PSEA Patent sealed
RENW Renewal (renewal fees accepted)

Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 29 OCT 2018 BY COMPUTER PACKAGES INC

Effective date: 20171003

RENW Renewal (renewal fees accepted)

Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 29 OCT 2019 BY COMPUTER PACKAGES INC

Effective date: 20181002

LAPS Patent lapsed