NZ303621A - A plasmid contains recombinant proteins such as human beta-casein with an encoded exogenous enzyme such as human kinase capable of phosphorylating recombinant beta-casein in a bacterial system - Google Patents

A plasmid contains recombinant proteins such as human beta-casein with an encoded exogenous enzyme such as human kinase capable of phosphorylating recombinant beta-casein in a bacterial system

Info

Publication number
NZ303621A
NZ303621A NZ303621A NZ30362196A NZ303621A NZ 303621 A NZ303621 A NZ 303621A NZ 303621 A NZ303621 A NZ 303621A NZ 30362196 A NZ30362196 A NZ 30362196A NZ 303621 A NZ303621 A NZ 303621A
Authority
NZ
New Zealand
Prior art keywords
casein
human
recombinant
plasmid
protein
Prior art date
Application number
NZ303621A
Other languages
English (en)
Inventor
Pradip Mukerji
Jennifer Marie Thurmond
Stig Lennart Hansson
Jeffrey Harris Baxter
Robert George Hards
Original Assignee
Abbott Lab
Symbicom Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/554,137 external-priority patent/US5942254A/en
Priority claimed from US08/554,642 external-priority patent/US5710044A/en
Application filed by Abbott Lab, Symbicom Ab filed Critical Abbott Lab
Publication of NZ303621A publication Critical patent/NZ303621A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4732—Casein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
NZ303621A 1995-02-27 1996-02-27 A plasmid contains recombinant proteins such as human beta-casein with an encoded exogenous enzyme such as human kinase capable of phosphorylating recombinant beta-casein in a bacterial system NZ303621A (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US39523995A 1995-02-27 1995-02-27
US08/554,137 US5942254A (en) 1995-02-27 1995-11-06 Phosphorylated recombinant human β-casein expressed in a bacterial system
US08/554,642 US5710044A (en) 1995-02-27 1995-11-06 Plasmid for expressing phosphorylated recombinant proteins in a bacterial system
PCT/US1996/002866 WO1996027018A1 (en) 1995-02-27 1996-02-27 A plasmid for expressing modified recombinant proteins in a bacterial system

Publications (1)

Publication Number Publication Date
NZ303621A true NZ303621A (en) 1999-04-29

Family

ID=27410154

Family Applications (1)

Application Number Title Priority Date Filing Date
NZ303621A NZ303621A (en) 1995-02-27 1996-02-27 A plasmid contains recombinant proteins such as human beta-casein with an encoded exogenous enzyme such as human kinase capable of phosphorylating recombinant beta-casein in a bacterial system

Country Status (5)

Country Link
EP (1) EP0812362A1 (de)
JP (1) JPH11500920A (de)
CA (1) CA2213734A1 (de)
NZ (1) NZ303621A (de)
WO (1) WO1996027018A1 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5707968A (en) * 1994-05-26 1998-01-13 Abbott Laboratories Inhibition of attachment of H.influenzae to human cells
US5942254A (en) * 1995-02-27 1999-08-24 Abbott Laboratories Phosphorylated recombinant human β-casein expressed in a bacterial system
ATE246934T1 (de) * 1995-11-06 2003-08-15 Abbott Lab Phosphoryliertes rekombinantes menschliches beta- kasein für die hemmung der anheftung von h. influenzae an menschlichen zellen
JP2000500141A (ja) * 1995-11-06 2000-01-11 アボツト・ラボラトリーズ リン酸化された組換えヒトベータ−カゼインを用いるヒト細胞に対するインフルエンザ菌の付着阻止方法
US6287866B1 (en) * 1996-11-27 2001-09-11 Abbott Laboratories β-casein expressing constructs

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0178863A1 (de) * 1984-10-15 1986-04-23 Schering Corporation Expressionssysteme die sich der Bakteriophage-T7-Promotoren bedienen und Gensequenzen
JPH06245775A (ja) * 1990-02-26 1994-09-06 Washington Univ 蛋白質ｎ−ミリストイル化方法
ATE158816T1 (de) * 1990-11-26 1997-10-15 Genetics Inst Expression von pace in wirtszellen und verfahren zu dessen verwendung
ES2107520T3 (es) * 1991-12-16 1997-12-01 Ciba Geigy Ag Endoproteasa dibasica recombinante situada en el reticulo endoplasmico y usos de la misma.
US5512434A (en) * 1992-12-14 1996-04-30 The United States Of America As Represented By The Department Of Health And Human Services Expression cloning of a human phosphatase

Also Published As

Publication number Publication date
WO1996027018A1 (en) 1996-09-06
EP0812362A1 (de) 1997-12-17
CA2213734A1 (en) 1996-09-06
JPH11500920A (ja) 1999-01-26

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