NO860180L - - Google Patents
Info
- Publication number
- NO860180L NO860180L NO860180A NO860180A NO860180L NO 860180 L NO860180 L NO 860180L NO 860180 A NO860180 A NO 860180A NO 860180 A NO860180 A NO 860180A NO 860180 L NO860180 L NO 860180L
- Authority
- NO
- Norway
- Prior art keywords
- cellulose
- cells
- fibers
- meq
- cell
- Prior art date
Links
- 210000004027 cell Anatomy 0.000 claims description 110
- 229920002678 cellulose Polymers 0.000 claims description 57
- 239000001913 cellulose Substances 0.000 claims description 57
- 239000000835 fiber Substances 0.000 claims description 56
- 210000002950 fibroblast Anatomy 0.000 claims description 18
- 229920003043 Cellulose fiber Polymers 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 15
- MZVQCMJNVPIDEA-UHFFFAOYSA-N [CH2]CN(CC)CC Chemical compound [CH2]CN(CC)CC MZVQCMJNVPIDEA-UHFFFAOYSA-N 0.000 claims description 13
- 238000005349 anion exchange Methods 0.000 claims description 13
- 230000012010 growth Effects 0.000 claims description 13
- 241000700605 Viruses Species 0.000 claims description 10
- 238000005342 ion exchange Methods 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 8
- 229960005486 vaccine Drugs 0.000 claims description 8
- 210000001840 diploid cell Anatomy 0.000 claims description 7
- 239000001963 growth medium Substances 0.000 claims description 7
- 239000000725 suspension Substances 0.000 claims description 7
- 239000006185 dispersion Substances 0.000 claims description 6
- -1 diethyl-(2-hydroxypropyl)aminoethyl Chemical group 0.000 claims description 5
- 125000000022 2-aminoethyl group Chemical group [H]C([*])([H])C([H])([H])N([H])[H] 0.000 claims description 4
- 210000005260 human cell Anatomy 0.000 claims description 4
- 239000002356 single layer Substances 0.000 claims description 4
- 241000124008 Mammalia Species 0.000 claims description 3
- 125000003277 amino group Chemical group 0.000 claims description 3
- 229920001425 Diethylaminoethyl cellulose Polymers 0.000 claims description 2
- 210000000712 G cell Anatomy 0.000 claims 1
- 239000002609 medium Substances 0.000 description 15
- 238000002474 experimental method Methods 0.000 description 14
- 239000000463 material Substances 0.000 description 13
- SEQKRHFRPICQDD-UHFFFAOYSA-N N-tris(hydroxymethyl)methylglycine Chemical compound OCC(CO)(CO)[NH2+]CC([O-])=O SEQKRHFRPICQDD-UHFFFAOYSA-N 0.000 description 10
- 238000004113 cell culture Methods 0.000 description 10
- 238000011534 incubation Methods 0.000 description 9
- 238000004114 suspension culture Methods 0.000 description 9
- 241000709701 Human poliovirus 1 Species 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 238000004809 thin layer chromatography Methods 0.000 description 6
- 239000007997 Tricine buffer Substances 0.000 description 5
- 150000001450 anions Chemical class 0.000 description 5
- 210000004072 lung Anatomy 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 4
- 239000006145 Eagle's minimal essential medium Substances 0.000 description 4
- 241000991587 Enterovirus C Species 0.000 description 4
- 206010028980 Neoplasm Diseases 0.000 description 4
- 229920002873 Polyethylenimine Polymers 0.000 description 4
- UZMAPBJVXOGOFT-UHFFFAOYSA-N Syringetin Natural products COC1=C(O)C(OC)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UZMAPBJVXOGOFT-UHFFFAOYSA-N 0.000 description 4
- 244000309466 calf Species 0.000 description 4
- 201000011510 cancer Diseases 0.000 description 4
- KCFYHBSOLOXZIF-UHFFFAOYSA-N dihydrochrysin Natural products COC1=C(O)C(OC)=CC(C2OC3=CC(O)=CC(O)=C3C(=O)C2)=C1 KCFYHBSOLOXZIF-UHFFFAOYSA-N 0.000 description 4
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 4
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 4
- 239000002953 phosphate buffered saline Substances 0.000 description 4
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 102000004142 Trypsin Human genes 0.000 description 3
- 108090000631 Trypsin Proteins 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 230000001419 dependent effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000012737 fresh medium Substances 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 210000001161 mammalian embryo Anatomy 0.000 description 3
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 3
- 235000017557 sodium bicarbonate Nutrition 0.000 description 3
- 229910001220 stainless steel Inorganic materials 0.000 description 3
- 239000010935 stainless steel Substances 0.000 description 3
- 239000012588 trypsin Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000282693 Cercopithecidae Species 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 2
- 150000001768 cations Chemical class 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 210000002257 embryonic structure Anatomy 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 229940076788 pyruvate Drugs 0.000 description 2
- 229940054269 sodium pyruvate Drugs 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- XSNSOQNHJHPRQG-UHFFFAOYSA-N 2-[bis(2-hydroxyethyl)amino]ethanol;2-(chloromethyl)oxirane Chemical compound ClCC1CO1.OCCN(CCO)CCO XSNSOQNHJHPRQG-UHFFFAOYSA-N 0.000 description 1
- 208000030507 AIDS Diseases 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 208000007212 Foot-and-Mouth Disease Diseases 0.000 description 1
- 241000710198 Foot-and-mouth disease virus Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000941423 Grom virus Species 0.000 description 1
- 241000709704 Human poliovirus 2 Species 0.000 description 1
- XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 201000005505 Measles Diseases 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 101100388690 Plasmodium falciparum (isolate K1 / Thailand) MEF-1 gene Proteins 0.000 description 1
- 208000000474 Poliomyelitis Diseases 0.000 description 1
- 229940124867 Poliovirus vaccine Drugs 0.000 description 1
- 206010037742 Rabies Diseases 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004873 anchoring Methods 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 125000003636 chemical group Chemical group 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 239000012847 fine chemical Substances 0.000 description 1
- 210000003953 foreskin Anatomy 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 206010022000 influenza Diseases 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000009343 monoculture Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000001338 necrotic effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 231100000590 oncogenic Toxicity 0.000 description 1
- 230000002246 oncogenic effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229960001539 poliomyelitis vaccine Drugs 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 208000009305 pseudorabies Diseases 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 201000005404 rubella Diseases 0.000 description 1
- 238000013376 serial cultivation Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 239000005723 virus inoculator Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
- C12N5/0075—General culture methods using substrates using microcarriers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/70—Polysaccharides
- C12N2533/78—Cellulose
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F02—COMBUSTION ENGINES; HOT-GAS OR COMBUSTION-PRODUCT ENGINE PLANTS
- F02B—INTERNAL-COMBUSTION PISTON ENGINES; COMBUSTION ENGINES IN GENERAL
- F02B61/00—Adaptations of engines for driving vehicles or for driving propellers; Combinations of engines with gearing
- F02B61/04—Adaptations of engines for driving vehicles or for driving propellers; Combinations of engines with gearing for driving propellers
- F02B61/045—Adaptations of engines for driving vehicles or for driving propellers; Combinations of engines with gearing for driving propellers for marine engines
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE8402742A SE454518B (sv) | 1984-05-21 | 1984-05-21 | Forfarande for odling av diploida celler i nervaro av cellulosafibrer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NO860180L true NO860180L (zh) | 1986-01-20 |
Family
ID=20355970
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO860180A NO860180L (zh) | 1984-05-21 | 1986-01-20 |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0216771A1 (zh) |
| JP (1) | JPS62500001A (zh) |
| DK (1) | DK19086A (zh) |
| NO (1) | NO860180L (zh) |
| SE (1) | SE454518B (zh) |
| WO (1) | WO1985005375A1 (zh) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3402927A1 (de) * | 1984-01-28 | 1985-08-08 | Pfeifer & Langen, 5000 Köln | Zellkulturmikrotraeger, verfahren zu seiner herstellung und seine verwendung zum zuechten von verankerungsabhaengigen zellen |
| US4874810A (en) * | 1986-03-07 | 1989-10-17 | General Electric Company | Impact modified polyphenylene ether-polyamide compositions |
| US9278730B2 (en) | 2009-12-30 | 2016-03-08 | Alex R. Kaye and Frances Kaye Trust | Brace for folding transom |
| US8539900B2 (en) | 2009-12-30 | 2013-09-24 | Alex R. Kaye and Frances Kaye Trust | Folding transom for a collapsible boat |
| JP5846550B2 (ja) * | 2011-05-02 | 2016-01-20 | 国立研究開発法人物質・材料研究機構 | 短繊維足場材料、短繊維−細胞複合凝集塊作製方法及び短繊維−細胞複合凝集塊 |
| TW201738256A (zh) * | 2016-04-04 | 2017-11-01 | 日產化學工業股份有限公司 | 蛋白質產生方法 |
| JP6799316B2 (ja) * | 2016-09-12 | 2020-12-16 | 国立研究開発法人物質・材料研究機構 | 配向性を有する短繊維−細胞複合凝集塊を製造するための方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZA776251B (en) * | 1976-11-11 | 1978-07-26 | Massachusetts Inst Technology | Improved cell culture microcarriers |
| FR2396083A1 (fr) * | 1977-06-30 | 1979-01-26 | Merieux Inst | Procede de culture de virus |
| US4352887A (en) * | 1979-10-29 | 1982-10-05 | Albert Einstein College Of Medicine Of Yeshiva University | Method and article for culturing differentiated cells |
| JPS59164723A (ja) * | 1983-03-10 | 1984-09-17 | Koken:Kk | 再生コラーゲンフイブリルを含有する基質及びその製造方法 |
-
1984
- 1984-05-21 SE SE8402742A patent/SE454518B/sv not_active IP Right Cessation
-
1985
- 1985-05-21 WO PCT/SE1985/000213 patent/WO1985005375A1/en not_active Application Discontinuation
- 1985-05-21 EP EP85902710A patent/EP0216771A1/en not_active Withdrawn
- 1985-05-21 JP JP60502558A patent/JPS62500001A/ja active Pending
-
1986
- 1986-01-15 DK DK19086A patent/DK19086A/da not_active Application Discontinuation
- 1986-01-20 NO NO860180A patent/NO860180L/no unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO1985005375A1 (en) | 1985-12-05 |
| DK19086A (da) | 1986-03-03 |
| SE8402742L (sv) | 1985-11-22 |
| SE8402742D0 (sv) | 1984-05-21 |
| DK19086D0 (da) | 1986-01-15 |
| EP0216771A1 (en) | 1987-04-08 |
| SE454518B (sv) | 1988-05-09 |
| JPS62500001A (ja) | 1987-01-08 |
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