NO324530B1 - Fremgangsmate for amplifisering og detektering av en malnukleinsyre - Google Patents
Fremgangsmate for amplifisering og detektering av en malnukleinsyre Download PDFInfo
- Publication number
- NO324530B1 NO324530B1 NO19971086A NO971086A NO324530B1 NO 324530 B1 NO324530 B1 NO 324530B1 NO 19971086 A NO19971086 A NO 19971086A NO 971086 A NO971086 A NO 971086A NO 324530 B1 NO324530 B1 NO 324530B1
- Authority
- NO
- Norway
- Prior art keywords
- target nucleic
- nucleic acid
- amplification
- primers
- amplified
- Prior art date
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- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 210000004408 hybridoma Anatomy 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 239000012678 infectious agent Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 229940115932 legionella pneumophila Drugs 0.000 description 1
- 125000005647 linker group Chemical group 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 159000000003 magnesium salts Chemical class 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 150000004712 monophosphates Chemical class 0.000 description 1
- JULKJDRBSRRBHT-UHFFFAOYSA-N n-(3-chloro-4-hydroxyphenyl)acetamide Chemical compound CC(=O)NC1=CC=C(O)C(Cl)=C1 JULKJDRBSRRBHT-UHFFFAOYSA-N 0.000 description 1
- 238000001821 nucleic acid purification Methods 0.000 description 1
- 229960003330 pentetic acid Drugs 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 150000008300 phosphoramidites Chemical class 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000000379 polymerizing effect Effects 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- 244000000040 protozoan parasite Species 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 238000000163 radioactive labelling Methods 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000004007 reversed phase HPLC Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- XZTJQQLJJCXOLP-UHFFFAOYSA-M sodium;decyl sulfate Chemical compound [Na+].CCCCCCCCCCOS([O-])(=O)=O XZTJQQLJJCXOLP-UHFFFAOYSA-M 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US61357196A | 1996-03-11 | 1996-03-11 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO971086D0 NO971086D0 (no) | 1997-03-10 |
| NO971086L NO971086L (no) | 1997-09-12 |
| NO324530B1 true NO324530B1 (no) | 2007-11-12 |
Family
ID=24457826
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO19971086A NO324530B1 (no) | 1996-03-11 | 1997-03-10 | Fremgangsmate for amplifisering og detektering av en malnukleinsyre |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US6280930B1 (fr) |
| EP (1) | EP0795612B1 (fr) |
| JP (1) | JP5000794B2 (fr) |
| KR (1) | KR100442196B1 (fr) |
| CN (1) | CN1309838C (fr) |
| AT (1) | ATE248928T1 (fr) |
| AU (1) | AU1514397A (fr) |
| CA (1) | CA2199213C (fr) |
| DE (1) | DE69724487T2 (fr) |
| DK (1) | DK0795612T3 (fr) |
| ES (1) | ES2205121T3 (fr) |
| NO (1) | NO324530B1 (fr) |
| PT (1) | PT795612E (fr) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AUPO524897A0 (en) * | 1997-02-21 | 1997-03-20 | Johnson & Johnson Research Pty. Limited | Method of amplifying specific nucleic acid target sequences |
| US6558901B1 (en) * | 1997-05-02 | 2003-05-06 | Biomerieux Vitek | Nucleic acid assays |
| IL124275A (en) * | 1997-05-02 | 2002-03-10 | Bio Merieux Vitek Inc | A method to produce sequences of nucleic acids |
| AU5882000A (en) | 1999-06-22 | 2001-01-09 | Invitrogen Corporation | Improved primers and methods for the detection and discrimination of nucleic acids |
| US6830902B1 (en) | 1999-07-02 | 2004-12-14 | Invitrogen Corporation | Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis |
| US20030165859A1 (en) | 2001-10-23 | 2003-09-04 | Invitrogen Corporation | Primers and methods for the detection and discrimination of nucleic acids |
| KR101329565B1 (ko) * | 2005-12-29 | 2013-11-14 | 케이맥(주) | Ｄｎａ 칩에 의한 1 단계 진단 |
| US8841069B2 (en) * | 2005-12-29 | 2014-09-23 | Korea Materials & Analysis Corporation | Dendron-mediated DNA virus detection |
| GB0806041D0 (en) | 2008-04-03 | 2008-05-14 | Genomica S A | Method for detection of herpesvirus in test sample |
| CN112322793A (zh) * | 2020-11-30 | 2021-02-05 | 中国科学院化学研究所 | 一种基于ccp-fret的rna病毒快速检测的方法 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US5635347A (en) * | 1986-04-30 | 1997-06-03 | Igen, Inc. | Rapid assays for amplification products |
| CA1317535C (fr) | 1987-06-30 | 1993-05-11 | Nanibhushan Dattagupta | Essai de sequences a l'aide de genes amplifies |
| US5229297A (en) * | 1989-02-03 | 1993-07-20 | Eastman Kodak Company | Containment cuvette for PCR and method of use |
| DE69030386T2 (de) * | 1989-12-22 | 1997-10-09 | F. Hoffmann-La Roche Ag, Basel | Bei hoher temperatur aktive reverse transkriptasen |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5344045A (en) * | 1990-12-17 | 1994-09-06 | The Coca-Cola Company | Liquid container system |
| CA2065719A1 (fr) * | 1991-04-30 | 1992-10-31 | John B. Findlay | Amplification d'acides nucleiques et methodes de detection faisant appel au cycle rapide de la reaction en chaine a la polymerase |
| IT1255737B (it) * | 1992-05-19 | 1995-11-15 | Reattore multitubolare a film cadente | |
| WO1994003635A1 (fr) * | 1992-08-04 | 1994-02-17 | Institute Of Molecular Biology & Biotechnology | Amplification et detection rapides d'acides nucleiques |
| US5645801A (en) * | 1993-10-21 | 1997-07-08 | Abbott Laboratories | Device and method for amplifying and detecting target nucleic acids |
| ATE226983T1 (de) * | 1994-08-19 | 2002-11-15 | Pe Corp Ny | Gekoppeltes ampflikation- und ligationverfahren |
-
1997
- 1997-03-05 CA CA002199213A patent/CA2199213C/fr not_active Expired - Fee Related
- 1997-03-06 AU AU15143/97A patent/AU1514397A/en not_active Abandoned
- 1997-03-10 KR KR1019970007874A patent/KR100442196B1/ko not_active IP Right Cessation
- 1997-03-10 NO NO19971086A patent/NO324530B1/no not_active IP Right Cessation
- 1997-03-11 PT PT97301591T patent/PT795612E/pt unknown
- 1997-03-11 DE DE69724487T patent/DE69724487T2/de not_active Expired - Lifetime
- 1997-03-11 AT AT97301591T patent/ATE248928T1/de active
- 1997-03-11 DK DK97301591T patent/DK0795612T3/da active
- 1997-03-11 ES ES97301591T patent/ES2205121T3/es not_active Expired - Lifetime
- 1997-03-11 EP EP97301591A patent/EP0795612B1/fr not_active Expired - Lifetime
- 1997-03-11 CN CNB971096201A patent/CN1309838C/zh not_active Expired - Fee Related
- 1997-03-11 JP JP05633797A patent/JP5000794B2/ja not_active Expired - Fee Related
- 1997-04-25 US US08/845,739 patent/US6280930B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| ATE248928T1 (de) | 2003-09-15 |
| JP5000794B2 (ja) | 2012-08-15 |
| JPH1033198A (ja) | 1998-02-10 |
| EP0795612A2 (fr) | 1997-09-17 |
| CA2199213A1 (fr) | 1997-09-11 |
| NO971086D0 (no) | 1997-03-10 |
| US6280930B1 (en) | 2001-08-28 |
| EP0795612B1 (fr) | 2003-09-03 |
| KR970065730A (ko) | 1997-10-13 |
| EP0795612A3 (fr) | 1999-03-24 |
| DE69724487T2 (de) | 2004-07-01 |
| DE69724487D1 (de) | 2003-10-09 |
| AU1514397A (en) | 1997-09-18 |
| NO971086L (no) | 1997-09-12 |
| CN1165862A (zh) | 1997-11-26 |
| KR100442196B1 (ko) | 2004-11-20 |
| CN1309838C (zh) | 2007-04-11 |
| PT795612E (pt) | 2004-02-27 |
| ES2205121T3 (es) | 2004-05-01 |
| DK0795612T3 (da) | 2003-12-15 |
| CA2199213C (fr) | 2007-04-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM1K | Lapsed by not paying the annual fees |