NO319885B1 - Fremgangsmate for fremstilling av rekombinante, adenoassosierte viruser (AAV), og deres anvendelse. - Google Patents
Fremgangsmate for fremstilling av rekombinante, adenoassosierte viruser (AAV), og deres anvendelse. Download PDFInfo
- Publication number
- NO319885B1 NO319885B1 NO19962950A NO962950A NO319885B1 NO 319885 B1 NO319885 B1 NO 319885B1 NO 19962950 A NO19962950 A NO 19962950A NO 962950 A NO962950 A NO 962950A NO 319885 B1 NO319885 B1 NO 319885B1
- Authority
- NO
- Norway
- Prior art keywords
- aav
- animal
- cell line
- gene
- genes
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 54
- 241000702421 Dependoparvovirus Species 0.000 title abstract description 18
- 238000002360 preparation method Methods 0.000 title description 6
- 230000008569 process Effects 0.000 title description 2
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 119
- 210000004027 cell Anatomy 0.000 claims description 92
- 241000700605 Viruses Species 0.000 claims description 57
- 239000013612 plasmid Substances 0.000 claims description 44
- 238000004519 manufacturing process Methods 0.000 claims description 37
- 241001465754 Metazoa Species 0.000 claims description 32
- 241000701161 unidentified adenovirus Species 0.000 claims description 23
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 19
- 238000001890 transfection Methods 0.000 claims description 15
- 210000004102 animal cell Anatomy 0.000 claims description 9
- 101150066583 rep gene Proteins 0.000 claims description 8
- 241001529453 unidentified herpesvirus Species 0.000 claims description 5
- 241000283690 Bos taurus Species 0.000 claims description 4
- 241001529936 Murinae Species 0.000 claims description 4
- 210000005260 human cell Anatomy 0.000 claims description 4
- 238000013518 transcription Methods 0.000 claims description 4
- 230000035897 transcription Effects 0.000 claims description 4
- 229960005486 vaccine Drugs 0.000 claims description 4
- 241000271566 Aves Species 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 101150104494 CAV1 gene Proteins 0.000 claims 1
- 101150047856 Cav2 gene Proteins 0.000 claims 1
- 239000013598 vector Substances 0.000 abstract description 11
- 238000001415 gene therapy Methods 0.000 abstract description 3
- 238000002659 cell therapy Methods 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 description 26
- 239000012634 fragment Substances 0.000 description 22
- 102000004169 proteins and genes Human genes 0.000 description 16
- 208000002267 Anti-neutrophil cytoplasmic antibody-associated vasculitis Diseases 0.000 description 15
- 238000010276 construction Methods 0.000 description 13
- 239000000047 product Substances 0.000 description 12
- 102100038909 Caveolin-2 Human genes 0.000 description 10
- 101000740981 Homo sapiens Caveolin-2 Proteins 0.000 description 10
- 238000001727 in vivo Methods 0.000 description 10
- 230000001225 therapeutic effect Effects 0.000 description 10
- 238000012546 transfer Methods 0.000 description 10
- BRZYSWJRSDMWLG-CAXSIQPQSA-N geneticin Chemical compound O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](C(C)O)O2)N)[C@@H](N)C[C@H]1N BRZYSWJRSDMWLG-CAXSIQPQSA-N 0.000 description 8
- 208000015181 infectious disease Diseases 0.000 description 8
- 241000702423 Adeno-associated virus - 2 Species 0.000 description 7
- 102000003951 Erythropoietin Human genes 0.000 description 7
- 108090000394 Erythropoietin Proteins 0.000 description 7
- 229940105423 erythropoietin Drugs 0.000 description 7
- OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 description 7
- 230000010076 replication Effects 0.000 description 7
- 238000000338 in vitro Methods 0.000 description 6
- 230000001939 inductive effect Effects 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 241000713333 Mouse mammary tumor virus Species 0.000 description 5
- 108010076504 Protein Sorting Signals Proteins 0.000 description 5
- 229910000389 calcium phosphate Inorganic materials 0.000 description 5
- 239000001506 calcium phosphate Substances 0.000 description 5
- 235000011010 calcium phosphates Nutrition 0.000 description 5
- 230000002950 deficient Effects 0.000 description 5
- 230000007170 pathology Effects 0.000 description 5
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 230000001413 cellular effect Effects 0.000 description 4
- 238000012761 co-transfection Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000006801 homologous recombination Effects 0.000 description 4
- 238000002744 homologous recombination Methods 0.000 description 4
- 210000000056 organ Anatomy 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 102100035888 Caveolin-1 Human genes 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 241000282412 Homo Species 0.000 description 3
- 101000715467 Homo sapiens Caveolin-1 Proteins 0.000 description 3
- 241001135569 Human adenovirus 5 Species 0.000 description 3
- 108700005077 Viral Genes Proteins 0.000 description 3
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 238000012217 deletion Methods 0.000 description 3
- 230000037430 deletion Effects 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 238000001638 lipofection Methods 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 230000006798 recombination Effects 0.000 description 3
- 238000005215 recombination Methods 0.000 description 3
- 238000006467 substitution reaction Methods 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 230000000381 tumorigenic effect Effects 0.000 description 3
- 230000003612 virological effect Effects 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- 101150044789 Cap gene Proteins 0.000 description 2
- 101710132601 Capsid protein Proteins 0.000 description 2
- 102000004594 DNA Polymerase I Human genes 0.000 description 2
- 108010017826 DNA Polymerase I Proteins 0.000 description 2
- 241000701533 Escherichia virus T4 Species 0.000 description 2
- 108090000368 Fibroblast growth factor 8 Proteins 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 238000000636 Northern blotting Methods 0.000 description 2
- 238000007792 addition Methods 0.000 description 2
- 230000000890 antigenic effect Effects 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000003937 drug carrier Substances 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000004520 electroporation Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 230000028993 immune response Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000002502 liposome Substances 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 238000002703 mutagenesis Methods 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000005162 pleiotrophin Human genes 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 231100000588 tumorigenic Toxicity 0.000 description 2
- 230000029812 viral genome replication Effects 0.000 description 2
- 102100022464 5'-nucleotidase Human genes 0.000 description 1
- 241001655883 Adeno-associated virus - 1 Species 0.000 description 1
- 241000202702 Adeno-associated virus - 3 Species 0.000 description 1
- 241000580270 Adeno-associated virus - 4 Species 0.000 description 1
- 208000005641 Adenomyosis Diseases 0.000 description 1
- -1 ApoAIV Proteins 0.000 description 1
- 102000007592 Apolipoproteins Human genes 0.000 description 1
- 108010071619 Apolipoproteins Proteins 0.000 description 1
- 102000013918 Apolipoproteins E Human genes 0.000 description 1
- 108010025628 Apolipoproteins E Proteins 0.000 description 1
- 108090000715 Brain-derived neurotrophic factor Proteins 0.000 description 1
- 102000004219 Brain-derived neurotrophic factor Human genes 0.000 description 1
- 108090000565 Capsid Proteins Proteins 0.000 description 1
- 201000009030 Carcinoma Diseases 0.000 description 1
- 102000053642 Catalytic RNA Human genes 0.000 description 1
- 108090000994 Catalytic RNA Proteins 0.000 description 1
- 102100032212 Caveolin-3 Human genes 0.000 description 1
- 102100025064 Cellular tumor antigen p53 Human genes 0.000 description 1
- 102100023321 Ceruloplasmin Human genes 0.000 description 1
- 108010005939 Ciliary Neurotrophic Factor Proteins 0.000 description 1
- 102100031614 Ciliary neurotrophic factor Human genes 0.000 description 1
- 101710094648 Coat protein Proteins 0.000 description 1
- 201000003883 Cystic fibrosis Diseases 0.000 description 1
- 102000000311 Cytosine Deaminase Human genes 0.000 description 1
- 108010080611 Cytosine Deaminase Proteins 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 102000001039 Dystrophin Human genes 0.000 description 1
- 108010069091 Dystrophin Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 108090000386 Fibroblast Growth Factor 1 Proteins 0.000 description 1
- 102100031706 Fibroblast growth factor 1 Human genes 0.000 description 1
- 102000004038 Glia Maturation Factor Human genes 0.000 description 1
- 108090000495 Glia Maturation Factor Proteins 0.000 description 1
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 description 1
- 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 description 1
- 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 description 1
- 108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 description 1
- 102000004457 Granulocyte-Macrophage Colony-Stimulating Factor Human genes 0.000 description 1
- 241000700721 Hepatitis B virus Species 0.000 description 1
- 101000678236 Homo sapiens 5'-nucleotidase Proteins 0.000 description 1
- 101000869042 Homo sapiens Caveolin-3 Proteins 0.000 description 1
- 101000599951 Homo sapiens Insulin-like growth factor I Proteins 0.000 description 1
- 101000611183 Homo sapiens Tumor necrosis factor Proteins 0.000 description 1
- 241000598171 Human adenovirus sp. Species 0.000 description 1
- 241000701044 Human gammaherpesvirus 4 Species 0.000 description 1
- 241000725303 Human immunodeficiency virus Species 0.000 description 1
- 102100037852 Insulin-like growth factor I Human genes 0.000 description 1
- 102100021244 Integral membrane protein GPR180 Human genes 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 102000015696 Interleukins Human genes 0.000 description 1
- 108010063738 Interleukins Proteins 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 102000008072 Lymphokines Human genes 0.000 description 1
- 108010074338 Lymphokines Proteins 0.000 description 1
- 108010046938 Macrophage Colony-Stimulating Factor Proteins 0.000 description 1
- 102000007651 Macrophage Colony-Stimulating Factor Human genes 0.000 description 1
- 101710125418 Major capsid protein Proteins 0.000 description 1
- 241000701168 Murine adenovirus 1 Species 0.000 description 1
- 101001055320 Myxine glutinosa Insulin-like growth factor Proteins 0.000 description 1
- 241001045988 Neogene Species 0.000 description 1
- 229930193140 Neomycin Natural products 0.000 description 1
- 108010025020 Nerve Growth Factor Proteins 0.000 description 1
- 102000015336 Nerve Growth Factor Human genes 0.000 description 1
- 108090000742 Neurotrophin 3 Proteins 0.000 description 1
- 108010089610 Nuclear Proteins Proteins 0.000 description 1
- 102000007999 Nuclear Proteins Human genes 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 101710141454 Nucleoprotein Proteins 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 108010039918 Polylysine Proteins 0.000 description 1
- 101710083689 Probable capsid protein Proteins 0.000 description 1
- 241000711798 Rabies lyssavirus Species 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 208000035415 Reinfection Diseases 0.000 description 1
- 101100379247 Salmo trutta apoa1 gene Proteins 0.000 description 1
- 206010039491 Sarcoma Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 102100032889 Sortilin Human genes 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 102000006601 Thymidine Kinase Human genes 0.000 description 1
- 108020004440 Thymidine kinase Proteins 0.000 description 1
- 102000044209 Tumor Suppressor Genes Human genes 0.000 description 1
- 108700025716 Tumor Suppressor Genes Proteins 0.000 description 1
- 102100040247 Tumor necrosis factor Human genes 0.000 description 1
- 108020005202 Viral DNA Proteins 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 241000701792 avian adenovirus Species 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000003914 blood derivative Substances 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 108091092356 cellular DNA Proteins 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- YTRQFSDWAXHJCC-UHFFFAOYSA-N chloroform;phenol Chemical compound ClC(Cl)Cl.OC1=CC=CC=C1 YTRQFSDWAXHJCC-UHFFFAOYSA-N 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 description 1
- 229960003957 dexamethasone Drugs 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 201000009274 endometriosis of uterus Diseases 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 208000037828 epithelial carcinoma Diseases 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 230000001024 immunotherapeutic effect Effects 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 239000007972 injectable composition Substances 0.000 description 1
- 229940047124 interferons Drugs 0.000 description 1
- 229940047122 interleukins Drugs 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 239000000644 isotonic solution Substances 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 101150091879 neo gene Proteins 0.000 description 1
- 229960004927 neomycin Drugs 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 239000002858 neurotransmitter agent Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 229940046166 oligodeoxynucleotide Drugs 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 229940021222 peritoneal dialysis isotonic solution Drugs 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 229920000656 polylysine Polymers 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 108091092562 ribozyme Proteins 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000009291 secondary effect Effects 0.000 description 1
- 230000009962 secretion pathway Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 239000013595 supernatant sample Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/505—Erythropoietin [EPO]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10341—Use of virus, viral particle or viral elements as a vector
- C12N2710/10344—Chimeric viral vector comprising heterologous viral elements for production of another viral vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14151—Methods of production or purification of viral material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Toxicology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9400934A FR2716682B1 (fr) | 1994-01-28 | 1994-01-28 | Procédé de préparation de virus adéno-associés (AAV) recombinants et utilisations. |
| PCT/FR1995/000054 WO1995020671A1 (fr) | 1994-01-28 | 1995-01-18 | Procede de preparation de virus adeno-associes (aav) recombinants et utilisations |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO962950D0 NO962950D0 (no) | 1996-07-12 |
| NO962950L NO962950L (no) | 1996-07-12 |
| NO319885B1 true NO319885B1 (no) | 2005-09-26 |
Family
ID=9459501
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO19962950A NO319885B1 (no) | 1994-01-28 | 1996-07-12 | Fremgangsmate for fremstilling av rekombinante, adenoassosierte viruser (AAV), og deres anvendelse. |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US5789390A (de) |
| EP (1) | EP0741793B1 (de) |
| JP (1) | JP3810078B2 (de) |
| KR (1) | KR100403708B1 (de) |
| AT (1) | ATE310097T1 (de) |
| AU (1) | AU706647B2 (de) |
| CA (1) | CA2181602A1 (de) |
| DE (1) | DE69534618T2 (de) |
| DK (1) | DK0741793T3 (de) |
| ES (1) | ES2252738T3 (de) |
| FI (1) | FI962990A0 (de) |
| FR (1) | FR2716682B1 (de) |
| IL (1) | IL112461A0 (de) |
| MX (1) | MX9603002A (de) |
| NO (1) | NO319885B1 (de) |
| WO (1) | WO1995020671A1 (de) |
| ZA (1) | ZA95628B (de) |
Families Citing this family (85)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6814962B1 (en) * | 1994-06-02 | 2004-11-09 | Aventis Pharma S.A. | Recombinant viruses and their use for treatment of atherosclerosis and other forms of coronary artery disease and method, reagent, and kit for evaluating susceptibility to same |
| US5658785A (en) * | 1994-06-06 | 1997-08-19 | Children's Hospital, Inc. | Adeno-associated virus materials and methods |
| US20020159979A1 (en) | 1994-06-06 | 2002-10-31 | Children's Hospital, Inc. | Adeno-associated virus materials and methods |
| US5856152A (en) * | 1994-10-28 | 1999-01-05 | The Trustees Of The University Of Pennsylvania | Hybrid adenovirus-AAV vector and methods of use therefor |
| FR2738575B1 (fr) * | 1995-09-08 | 1997-10-10 | Centre Nat Rech Scient | Cellules pour la production d'adenovirus recombinants |
| IL116816A (en) * | 1995-01-20 | 2003-05-29 | Rhone Poulenc Rorer Sa | Cell for the production of a defective recombinant adenovirus or an adeno-associated virus and the various uses thereof |
| US6281010B1 (en) * | 1995-06-05 | 2001-08-28 | The Trustees Of The University Of Pennsylvania | Adenovirus gene therapy vehicle and cell line |
| US5756283A (en) * | 1995-06-05 | 1998-05-26 | The Trustees Of The University Of Pennsylvania | Method for improved production of recombinant adeno-associated viruses for gene therapy |
| FR2735789B1 (fr) | 1995-06-23 | 1997-07-25 | Centre Nat Rech Scient | Adenovirus recombinants, leur utilisation pour preparer des aav, lignee cellulaire complementaire et compositions pharmaceutiques les contenant |
| US6207457B1 (en) * | 1995-09-08 | 2001-03-27 | Avigen, Inc. | Targeted nucleotide sequence delivery and integration system |
| US6162796A (en) * | 1995-09-27 | 2000-12-19 | The Rockefeller University | Method for transferring genes to the heart using AAV vectors |
| US20060088938A1 (en) * | 1995-12-15 | 2006-04-27 | Mitchell Lloyd G | Methods and compositions for use in spliceosome mediated RNA trans-splicing in plants |
| US20030027250A1 (en) * | 1995-12-15 | 2003-02-06 | Mitchell Lloyd G. | Methods and compositions for use in spliceosome mediated RNA trans-splicing |
| US5846528A (en) * | 1996-01-18 | 1998-12-08 | Avigen, Inc. | Treating anemia using recombinant adeno-associated virus virions comprising an EPO DNA sequence |
| US5858351A (en) | 1996-01-18 | 1999-01-12 | Avigen, Inc. | Methods for delivering DNA to muscle cells using recombinant adeno-associated virus vectors |
| US5962313A (en) | 1996-01-18 | 1999-10-05 | Avigen, Inc. | Adeno-associated virus vectors comprising a gene encoding a lyosomal enzyme |
| US20020037867A1 (en) * | 1999-02-26 | 2002-03-28 | James M. Wilson | Method for recombinant adeno-associated virus-directed gene therapy |
| US5866552A (en) * | 1996-09-06 | 1999-02-02 | The Trustees Of The University Of Pennsylvania | Method for expressing a gene in the absence of an immune response |
| EP0928202A4 (de) * | 1996-09-25 | 2001-09-05 | Cell Genesys Inc | Nicht-invasive verabreichung von adeno-assoziierten viralen vektoren |
| EP0950091A2 (de) * | 1996-12-18 | 1999-10-20 | Targeted Genetics Corporation | Aav-"split-packaging"-gene und diese gene enthaltende zelllinien zur verwendung in der herstellung von rekombinanten aav-vektoren |
| US20040161409A1 (en) * | 1997-07-25 | 2004-08-19 | Kurtzman Gary J. | Induction of immune response to antigens expressed by recombinant adeno-associated virus |
| US6242426B1 (en) * | 1997-07-25 | 2001-06-05 | Avigen, Inc. | Induction of immune response to antigens expressed by recombinant adeno-associated virus |
| US20030125278A1 (en) * | 1997-08-13 | 2003-07-03 | Tang De-Chu C. | Immunization of animals by topical applications of a salmonella-based vector |
| US6706693B1 (en) | 1997-08-13 | 2004-03-16 | The Uab Research Foundation | Vaccination by topical application of genetic vectors |
| US20030045492A1 (en) * | 1997-08-13 | 2003-03-06 | Tang De-Chu C. | Vaccination by topical application of recombinant vectors |
| US6348450B1 (en) * | 1997-08-13 | 2002-02-19 | The Uab Research Foundation | Noninvasive genetic immunization, expression products therefrom and uses thereof |
| US6716823B1 (en) | 1997-08-13 | 2004-04-06 | The Uab Research Foundation | Noninvasive genetic immunization, expression products therefrom, and uses thereof |
| US6251677B1 (en) | 1997-08-25 | 2001-06-26 | The Trustees Of The University Of Pennsylvania | Hybrid adenovirus-AAV virus and methods of use thereof |
| US6642051B1 (en) | 1997-10-21 | 2003-11-04 | Targeted Genetics Corporation | Amplifiable adeno-associated virus(AAV) packaging cassettes for the production of recombinant AAV vectors |
| WO1999028346A1 (de) * | 1997-12-03 | 1999-06-10 | Roche Diagnostics Gmbh | Erythropoietin mit hoher spezifischer aktivität |
| DE19830141A1 (de) * | 1998-07-06 | 2000-01-13 | Regine Heilbronn | Rekombinante Herpesviren für die Erzeugung rekombinanter Adeno-Assoziierter-Viren |
| US6303362B1 (en) * | 1998-11-19 | 2001-10-16 | The Board Of Trustees Of The Leland Stanford Junior University | Adenoviral vector and methods for making and using the same |
| US6759050B1 (en) | 1998-12-03 | 2004-07-06 | Avigen, Inc. | Excipients for use in adeno-associated virus pharmaceutical formulations, and pharmaceutical formulations made therewith |
| US6387368B1 (en) | 1999-02-08 | 2002-05-14 | The Trustees Of The University Of Pennsylvania | Hybrid adenovirus-AAV virus and methods of use thereof |
| DE19905501B4 (de) * | 1999-02-10 | 2005-05-19 | MediGene AG, Gesellschaft für molekularbiologische Kardiologie und Onkologie | Verfahren zur Herstellung eines rekombinanten Adeno-assoziierten Virus, geeignete Mittel hierzu sowie Verwendung zur Herstellung eines Arzneimittels |
| US20040043488A1 (en) * | 1999-02-17 | 2004-03-04 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | Adeno-associated viral gene-transfer vector system |
| US6893865B1 (en) | 1999-04-28 | 2005-05-17 | Targeted Genetics Corporation | Methods, compositions, and cells for encapsidating recombinant vectors in AAV particles |
| US20040009936A1 (en) * | 1999-05-03 | 2004-01-15 | Tang De-Chu C. | Vaccine and drug delivery by topical application of vectors and vector extracts |
| US7208315B2 (en) | 2000-01-05 | 2007-04-24 | Fred Hutchinson Cancer Research Center | Compositions and methods for efficient AAV vector production |
| ATE377081T1 (de) * | 2000-02-25 | 2007-11-15 | Ludwig Inst Cancer Res | Material und verfahren die hybridvaskularendothelwachstumfaktoren dns und proteine enthalten und screeningverfahren für modulatoren |
| JP2004536553A (ja) * | 2000-09-30 | 2004-12-09 | ディヴァーサ コーポレイション | 始原ゲノムの本質部分突然変異、突然変異の組合せおよび任意反復による全細胞工学 |
| US6916635B2 (en) * | 2000-10-02 | 2005-07-12 | The Research Foundation Of State University Of New York | Hybrid adenovirus/adeno-associated virus vectors and methods of use thereof |
| US20040126774A1 (en) * | 2001-01-08 | 2004-07-01 | Mitchell Lioyd G. | Correction of factor VIII genetic defects using spliceosome mediated RNA trans splicing |
| WO2002082904A2 (en) | 2001-04-13 | 2002-10-24 | The Trustees Of The University Of Pennsylvania | Method of treating or retarding the development of blindness |
| US6838285B2 (en) | 2001-09-18 | 2005-01-04 | Becton Dickinson | Site specific recombinase based method for producing adenoviral vectors |
| US7399753B2 (en) * | 2002-02-25 | 2008-07-15 | Virxsys Corporation | Trans-splicing mediated photodynamic therapy |
| JP2005536231A (ja) * | 2002-05-08 | 2005-12-02 | イントロン，インコーポレーテッド | アデノウイルスに細胞選択的複製をもたらすための、スプライセオソーム媒介型ｒｎａトランススプライシングの使用 |
| JP4654033B2 (ja) * | 2002-10-23 | 2011-03-16 | バークシス コーポレーション | 効率的なプレ−トランス−スプライシング分子の同定のためのスクリーニング方法 |
| JP2007505603A (ja) * | 2003-09-12 | 2007-03-15 | ヴァーテックス ファーマシューティカルズ、 インコーポレイテッド | プロテアーゼ活性および肝障害のための動物モデル |
| CA2553828A1 (en) * | 2004-01-23 | 2005-08-04 | Intronn, Inc. | Expression of apoa-1 and variants thereof using spliceosome mediated rna trans-splicing |
| US7968334B2 (en) * | 2004-01-23 | 2011-06-28 | Virxsys Corporation | Expression of apoAI and variants thereof using spliceosome mediated RNA trans-splicing |
| US8053232B2 (en) * | 2004-01-23 | 2011-11-08 | Virxsys Corporation | Correction of alpha-1-antitrypsin genetic defects using spliceosome mediated RNA trans splicing |
| EP1718738A2 (de) * | 2004-02-23 | 2006-11-08 | Crucell Holland B.V. | Verfahren zur reinigung von viren |
| DE602005027673D1 (de) * | 2004-03-05 | 2011-06-09 | Vegenics Pty Ltd | Materialien und verfahren für wachstumsfaktorbindende konstrukte |
| US7582442B2 (en) * | 2004-03-16 | 2009-09-01 | The Regents Of The University Of Michigan | Methods and compositions for using aleveolar macrophage phospholipase A2 |
| US7319015B2 (en) * | 2004-03-16 | 2008-01-15 | The Regents Of The University Of Michigan | Methods and compositions for using alveolar macrophage phospholipase A2 |
| WO2006033689A2 (en) * | 2004-06-18 | 2006-03-30 | The University Of Montana | Aav mediated gene delivery to cochlear cells |
| WO2006014798A2 (en) * | 2004-07-27 | 2006-02-09 | Mount Sinai School Of Medicine | Methods and compositions for using sax2 |
| US20060094110A1 (en) * | 2004-07-30 | 2006-05-04 | Mcgarrity Gerard J | Use of spliceosome mediated RNA trans-splicing for immunotherapy |
| US20060134658A1 (en) * | 2004-08-09 | 2006-06-22 | Garcia-Blanco Mariano A | Use of RNA trans-splicing for generation of interfering RNA molecules |
| FI20050753A (fi) | 2004-09-03 | 2006-03-04 | Licentia Oy | Uudet peptidit |
| DE102004047492B4 (de) * | 2004-09-23 | 2006-07-20 | Jost-Werke Gmbh & Co. Kg | Verfahren zum Übertragen von elektrischer, pneumatischer oder hydraulischer Energie sowie ein Energieübertragungssystem |
| US7871795B2 (en) | 2004-10-08 | 2011-01-18 | Virxsys Corporation | Targeted trans-splicing of highly abundant transcripts for in vivo production of recombinant proteins |
| AU2005326784B2 (en) * | 2004-10-08 | 2012-03-15 | Virxsys Corporation | Use of RNA trans-splicing for antibody gene transfer and antibody polypeptide production |
| US7531523B2 (en) | 2005-02-17 | 2009-05-12 | Vertex Pharmaceuticals Incorporated | Sodium channel protein type III alpha-subunit splice variant |
| WO2006091722A2 (en) * | 2005-02-23 | 2006-08-31 | Uab Research Foundation | Alkyl-glycoside enhanced vaccination |
| CA2602944C (en) * | 2005-04-11 | 2015-08-11 | Crucell Holland B.V. | Virus purification using ultrafiltration |
| AU2006279462A1 (en) | 2005-08-15 | 2007-02-22 | Vegenics Limited | Modified VEGF and PDGF with improved angiogenic properties |
| US7972813B2 (en) * | 2005-09-30 | 2011-07-05 | Vertex Pharmaceuticals Incorporated | Tetrodotoxin-resistant sodium channel alpha subunit |
| US20080200408A1 (en) * | 2005-09-30 | 2008-08-21 | Mccormack Kenneth | Deletion mutants of tetrodotoxin-resistant sodium channel alpha subunit |
| DE602005023550D1 (de) | 2005-12-14 | 2010-10-21 | Licentia Ltd | Verwendungen eines neurotrophischen Faktors |
| WO2007089780A2 (en) * | 2006-01-30 | 2007-08-09 | Licentia, Ltd. | Bmx/etk tyrosine kinase gene therapy materials and methods |
| DK2548578T3 (da) | 2006-05-17 | 2014-10-06 | Ludwig Inst Cancer Res | Målretning af VEGF-B-regulering af fedtsyretransportører til modulering af humane sygdomme |
| FI20070808A0 (fi) | 2007-10-25 | 2007-10-25 | Mart Saarma | GDNF:n silmukointivariantit ja niiden käytöt |
| US20090196854A1 (en) * | 2008-02-04 | 2009-08-06 | Kytos Biosystems S.A. | Methods and compositions for use of crl 5803 cells for expression of biotherapeutics and encapsulated cell-based delivery |
| FI20080326A0 (fi) | 2008-04-30 | 2008-04-30 | Licentia Oy | Neurotroofinen tekijä MANF ja sen käytöt |
| US8052970B2 (en) * | 2008-06-30 | 2011-11-08 | The Regents Of The University Of Michigan | Lysosomal phospholipase A2 (LPLA2) activity as a diagnostic and therapeutic target for identifying and treating systemic lupus erythematosis |
| WO2012158989A2 (en) | 2011-05-19 | 2012-11-22 | The Regents Of The University Of Michigan | Integrin alpha-2 binding agents and use thereof to inhibit cancer cell proliferation |
| DK2785683T3 (da) | 2011-11-30 | 2020-04-14 | Ludwig Inst For Cancer Res Ltd | Inkt-cellemodulatorer og fremgangsmåder til anvendelse heraf |
| GB201120860D0 (en) | 2011-12-05 | 2012-01-18 | Cambridge Entpr Ltd | Cancer immunotherapy |
| WO2013184209A1 (en) | 2012-06-04 | 2013-12-12 | Ludwig Institute For Cancer Research Ltd. | Mif for use in methods of treating subjects with a neurodegenerative disorder |
| EP3421602B1 (de) | 2012-09-06 | 2021-03-03 | The University of Chicago | Antisense-polynukleotide zur induktion von exon-skipping sowie verfahren zur behandlung von muskeldystrophien |
| WO2014124487A1 (en) | 2013-02-18 | 2014-08-21 | Vegenics Pty Limited | Ligand binding molecules and uses thereof |
| WO2014191630A2 (en) | 2013-05-28 | 2014-12-04 | Helsingin Yliopisto | Non-human animal model encoding a non-functional manf gene |
| WO2016164602A1 (en) | 2015-04-08 | 2016-10-13 | The University Of Chicago | Compositions and methods for correcting limb girdle muscular dystrophy type 2c using exon skipping |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5139941A (en) * | 1985-10-31 | 1992-08-18 | University Of Florida Research Foundation, Inc. | AAV transduction vectors |
| WO1991018088A1 (en) * | 1990-05-23 | 1991-11-28 | The United States Of America, Represented By The Secretary, United States Department Of Commerce | Adeno-associated virus (aav)-based eucaryotic vectors |
| US5173414A (en) * | 1990-10-30 | 1992-12-22 | Applied Immune Sciences, Inc. | Production of recombinant adeno-associated virus vectors |
-
1994
- 1994-01-28 FR FR9400934A patent/FR2716682B1/fr not_active Expired - Fee Related
-
1995
- 1995-01-18 WO PCT/FR1995/000054 patent/WO1995020671A1/fr active IP Right Grant
- 1995-01-18 CA CA002181602A patent/CA2181602A1/fr not_active Abandoned
- 1995-01-18 EP EP95907045A patent/EP0741793B1/de not_active Expired - Lifetime
- 1995-01-18 MX MX9603002A patent/MX9603002A/es not_active IP Right Cessation
- 1995-01-18 ES ES95907045T patent/ES2252738T3/es not_active Expired - Lifetime
- 1995-01-18 US US08/682,520 patent/US5789390A/en not_active Expired - Fee Related
- 1995-01-18 DK DK95907045T patent/DK0741793T3/da active
- 1995-01-18 KR KR1019960704129A patent/KR100403708B1/ko not_active IP Right Cessation
- 1995-01-18 AT AT95907045T patent/ATE310097T1/de not_active IP Right Cessation
- 1995-01-18 JP JP51990995A patent/JP3810078B2/ja not_active Expired - Fee Related
- 1995-01-18 AU AU15395/95A patent/AU706647B2/en not_active Ceased
- 1995-01-18 DE DE69534618T patent/DE69534618T2/de not_active Expired - Fee Related
- 1995-01-26 ZA ZA95628A patent/ZA95628B/xx unknown
- 1995-01-26 IL IL11246195A patent/IL112461A0/xx active IP Right Grant
-
1996
- 1996-07-12 NO NO19962950A patent/NO319885B1/no unknown
- 1996-07-26 FI FI962990A patent/FI962990A0/fi not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| FI962990A (fi) | 1996-07-26 |
| FR2716682B1 (fr) | 1996-04-26 |
| DK0741793T3 (da) | 2006-03-27 |
| ZA95628B (en) | 1995-10-23 |
| AU706647B2 (en) | 1999-06-17 |
| JPH09509051A (ja) | 1997-09-16 |
| CA2181602A1 (fr) | 1995-08-03 |
| KR100403708B1 (ko) | 2004-05-31 |
| EP0741793A1 (de) | 1996-11-13 |
| MX9603002A (es) | 1997-06-28 |
| DE69534618D1 (de) | 2005-12-22 |
| ATE310097T1 (de) | 2005-12-15 |
| AU1539595A (en) | 1995-08-15 |
| JP3810078B2 (ja) | 2006-08-16 |
| ES2252738T3 (es) | 2006-05-16 |
| DE69534618T2 (de) | 2006-07-27 |
| EP0741793B1 (de) | 2005-11-16 |
| NO962950D0 (no) | 1996-07-12 |
| FI962990A0 (fi) | 1996-07-26 |
| IL112461A0 (en) | 1995-03-30 |
| FR2716682A1 (fr) | 1995-09-01 |
| NO962950L (no) | 1996-07-12 |
| WO1995020671A1 (fr) | 1995-08-03 |
| US5789390A (en) | 1998-08-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| NO319885B1 (no) | Fremgangsmate for fremstilling av rekombinante, adenoassosierte viruser (AAV), og deres anvendelse. | |
| KR100514070B1 (ko) | 재조합아데노바이러스,에이에이브이제조를위한이의용도,상보성세포주및이러한아데노바이러스를함유하는약학조성물 | |
| KR100510822B1 (ko) | 재조합 아데노바이러스 제조용 세포 | |
| RU2233333C2 (ru) | Вирусные векторы и их применение в генной терапии | |
| US6482617B2 (en) | Viable contaminant particle free adenoviruses, their preparation and use | |
| Kremer et al. | Adenovirus and adeno-associated virus mediated gene transfer | |
| ES2310924T3 (es) | Vectores adenovirales defectivos y utilizacion en terapia genica. | |
| JP4376454B2 (ja) | アデノウイルスベクター及び相同組換えイベントの低減方法 | |
| US6521426B1 (en) | Preparation of recombinant adenovirus carrying a rep gene of adeno-associated virus | |
| CN112638936A (zh) | 腺病毒多核苷酸和多肽 | |
| KR100484441B1 (ko) | IVa2유전자가불활성화된결손재조합아데노바이러스 | |
| MXPA97001766A (en) | Defective recombinant adenovirus with a vat iva2 inactiv | |
| CN115151647A (zh) | Dna扩增方法 | |
| JP2004519201A (ja) | キメラアデノウイルスベクター | |
| AU752811B2 (en) | Preparation of recombinant adenovirus carrying a rep gene of Adeno-Associated Virus | |
| US7687615B2 (en) | PAV regions for encapsidation and E1 transcriptional control |