NO317703B1 - Fremgangsmate for redusering av oppdriften av en mikroorganisme; fremgangsmate for dispergering av en klump av mikroorganismer, kit, anvendelse av kit'et og anvendelse av en tilnaermet-oktadekyldetergent - Google Patents

Info

Publication number

NO317703B1

NO317703B1 NO19964187A NO964187A NO317703B1 NO 317703 B1 NO317703 B1 NO 317703B1 NO 19964187 A NO19964187 A NO 19964187A NO 964187 A NO964187 A NO 964187A NO 317703 B1 NO317703 B1 NO 317703B1

Authority

NO

Norway

Prior art keywords

cas

dimethyl

inner salt

detergent

phosphonooxy

Prior art date

1994-04-05

Links

• Espacenet
• Global Dossier
• Discuss

• 238000000034 method Methods 0.000 title claims abstract description 286
• 239000003599 detergent Substances 0.000 title claims description 526
• 244000005700 microbiome Species 0.000 title claims description 84
• 230000008569 process Effects 0.000 title claims description 17
• 238000001514 detection method Methods 0.000 claims abstract description 83
• 238000002360 preparation method Methods 0.000 claims abstract description 20
• 150000003839 salts Chemical class 0.000 claims description 286
• KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims description 181
• 229960003237 betaine Drugs 0.000 claims description 178
• 125000000217 alkyl group Chemical group 0.000 claims description 135
• -1 -C2<H>5 Chemical group 0.000 claims description 129
• 238000012545 processing Methods 0.000 claims description 108
• 239000002253 acid Substances 0.000 claims description 86
• 201000008827 tuberculosis Diseases 0.000 claims description 77
• 241000186367 Mycobacterium avium Species 0.000 claims description 60
• 238000011084 recovery Methods 0.000 claims description 58
• 238000005406 washing Methods 0.000 claims description 52
• CUEQHYJSSUSIFI-UHFFFAOYSA-N corynomycolic acid Chemical compound CCCCCCCCCCCCCCCC(O)C(C(O)=O)CCCCCCCCCCCCCC CUEQHYJSSUSIFI-UHFFFAOYSA-N 0.000 claims description 42
• 239000012528 membrane Substances 0.000 claims description 33
• 125000004079 stearyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 claims description 30
• DIROHOMJLWMERM-UHFFFAOYSA-N 3-[dimethyl(octadecyl)azaniumyl]propane-1-sulfonate Chemical compound CCCCCCCCCCCCCCCCCC[N+](C)(C)CCCS([O-])(=O)=O DIROHOMJLWMERM-UHFFFAOYSA-N 0.000 claims description 29
• 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 29
• 241000894007 species Species 0.000 claims description 29
• 229940117986 sulfobetaine Drugs 0.000 claims description 25
• 239000003795 chemical substances by application Substances 0.000 claims description 24
• 238000000338 in vitro Methods 0.000 claims description 24
• 229910052757 nitrogen Inorganic materials 0.000 claims description 23
• 125000004432 carbon atom Chemical group C* 0.000 claims description 22
• 239000006185 dispersion Substances 0.000 claims description 22
• TUBRCQBRKJXJEA-UHFFFAOYSA-N 3-[hexadecyl(dimethyl)azaniumyl]propane-1-sulfonate Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)CCCS([O-])(=O)=O TUBRCQBRKJXJEA-UHFFFAOYSA-N 0.000 claims description 21
• 241000187482 Mycobacterium avium subsp. paratuberculosis Species 0.000 claims description 21
• 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 claims description 20
• 241000186363 Mycobacterium kansasii Species 0.000 claims description 18
• 238000003745 diagnosis Methods 0.000 claims description 18
• PSBDWGZCVUAZQS-UHFFFAOYSA-N (dimethylsulfonio)acetate Chemical compound C[S+](C)CC([O-])=O PSBDWGZCVUAZQS-UHFFFAOYSA-N 0.000 claims description 17
• 125000001570 methylene group Chemical group [H]C([H])([*:1])[*:2] 0.000 claims description 17
• 239000003760 tallow Substances 0.000 claims description 16
• BHATUINFZWUDIX-UHFFFAOYSA-N Zwittergent 3-14 Chemical compound CCCCCCCCCCCCCC[N+](C)(C)CCCS([O-])(=O)=O BHATUINFZWUDIX-UHFFFAOYSA-N 0.000 claims description 15
• 125000000816 ethylene group Chemical group [H]C([H])([*:1])C([H])([H])[*:2] 0.000 claims description 13
• 241000186364 Mycobacterium intracellulare Species 0.000 claims description 12
• 241000187492 Mycobacterium marinum Species 0.000 claims description 12
• 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 claims description 12
• 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims description 12
• IZWSFJTYBVKZNK-UHFFFAOYSA-N lauryl sulfobetaine Chemical compound CCCCCCCCCCCC[N+](C)(C)CCCS([O-])(=O)=O IZWSFJTYBVKZNK-UHFFFAOYSA-N 0.000 claims description 11
• 241000186366 Mycobacterium bovis Species 0.000 claims description 10
• 241000187484 Mycobacterium gordonae Species 0.000 claims description 10
• 230000008859 change Effects 0.000 claims description 10
• VQFWSIABHIHJBG-UHFFFAOYSA-N 3-[3-(dodecanoylamino)propyl-dimethylazaniumyl]propane-1-sulfonate Chemical compound CCCCCCCCCCCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O VQFWSIABHIHJBG-UHFFFAOYSA-N 0.000 claims description 9
• ZFVNBVQBIZJUEY-UHFFFAOYSA-N 4-[dodecyl(dimethyl)azaniumyl]butanoate Chemical compound CCCCCCCCCCCC[N+](C)(C)CCCC([O-])=O ZFVNBVQBIZJUEY-UHFFFAOYSA-N 0.000 claims description 9
• 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 9
• 230000007935 neutral effect Effects 0.000 claims description 9
• QVRMIJZFODZFNE-UHFFFAOYSA-N 2-[dimethyl-[3-(octadecanoylamino)propyl]azaniumyl]acetate Chemical compound CCCCCCCCCCCCCCCCCC(=O)NCCC[N+](C)(C)CC([O-])=O QVRMIJZFODZFNE-UHFFFAOYSA-N 0.000 claims description 8
• IXOCGRPBILEGOX-UHFFFAOYSA-N 3-[3-(dodecanoylamino)propyl-dimethylazaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound CCCCCCCCCCCC(=O)NCCC[N+](C)(C)CC(O)CS([O-])(=O)=O IXOCGRPBILEGOX-UHFFFAOYSA-N 0.000 claims description 8
• FFXBFCULVYPLML-UHFFFAOYSA-N 4-[hexadecyl(dimethyl)azaniumyl]butanoate Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)CCCC([O-])=O FFXBFCULVYPLML-UHFFFAOYSA-N 0.000 claims description 8
• WKALLSVICJPZTM-UHFFFAOYSA-N 3-[decyl(dimethyl)azaniumyl]propane-1-sulfonate Chemical compound CCCCCCCCCC[N+](C)(C)CCCS([O-])(=O)=O WKALLSVICJPZTM-UHFFFAOYSA-N 0.000 claims description 7
• 241000186362 Mycobacterium leprae Species 0.000 claims description 7
• 241000187493 Mycobacterium malmoense Species 0.000 claims description 7
• 241000187496 Mycobacterium szulgai Species 0.000 claims description 7
• 241000187494 Mycobacterium xenopi Species 0.000 claims description 7
• TYIOVYZMKITKRO-UHFFFAOYSA-N 2-[hexadecyl(dimethyl)azaniumyl]acetate Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)CC([O-])=O TYIOVYZMKITKRO-UHFFFAOYSA-N 0.000 claims description 6
• CLCSYZQBLQDRQU-UHFFFAOYSA-N 3-[3-(hexadecanoylamino)propyl-dimethylazaniumyl]propane-1-sulfonate Chemical compound CCCCCCCCCCCCCCCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O CLCSYZQBLQDRQU-UHFFFAOYSA-N 0.000 claims description 6
• PRWOYYDJCJITLW-UHFFFAOYSA-N 3-[dimethyl(tetradecyl)azaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound CCCCCCCCCCCCCC[N+](C)(C)CC(O)CS([O-])(=O)=O PRWOYYDJCJITLW-UHFFFAOYSA-N 0.000 claims description 6
• OSPOJLWAJPWJTO-UHFFFAOYSA-N 3-[hexadecyl(dimethyl)azaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)CC(O)CS([O-])(=O)=O OSPOJLWAJPWJTO-UHFFFAOYSA-N 0.000 claims description 6
• 241000187478 Mycobacterium chelonae Species 0.000 claims description 6
• 241000186365 Mycobacterium fortuitum Species 0.000 claims description 6
• 241000187485 Mycobacterium gastri Species 0.000 claims description 6
• 125000004429 atom Chemical group 0.000 claims description 6
• MRUAUOIMASANKQ-UHFFFAOYSA-N cocamidopropyl betaine Chemical compound CCCCCCCCCCCC(=O)NCCC[N+](C)(C)CC([O-])=O MRUAUOIMASANKQ-UHFFFAOYSA-N 0.000 claims description 6
• ZMXWTYDZWPGTOM-LKAWRWRFSA-N 2-[3-[[(z,12r)-12-hydroxyoctadec-9-enoyl]amino]propyl-dimethylazaniumyl]acetate Chemical compound CCCCCC[C@@H](O)C\C=C/CCCCCCCC(=O)NCCC[N+](C)(C)CC([O-])=O ZMXWTYDZWPGTOM-LKAWRWRFSA-N 0.000 claims description 5
• AMRBZKOCOOPYNY-QXMHVHEDSA-N 2-[dimethyl-[(z)-octadec-9-enyl]azaniumyl]acetate Chemical compound CCCCCCCC\C=C/CCCCCCCC[N+](C)(C)CC([O-])=O AMRBZKOCOOPYNY-QXMHVHEDSA-N 0.000 claims description 5
• AEDQNOLIADXSBB-UHFFFAOYSA-N 3-(dodecylazaniumyl)propanoate Chemical compound CCCCCCCCCCCCNCCC(O)=O AEDQNOLIADXSBB-UHFFFAOYSA-N 0.000 claims description 5
• QZRAABPTWGFNIU-UHFFFAOYSA-N 3-[dimethyl(octyl)azaniumyl]propane-1-sulfonate Chemical compound CCCCCCCC[N+](C)(C)CCCS([O-])(=O)=O QZRAABPTWGFNIU-UHFFFAOYSA-N 0.000 claims description 5
• QDDVYXATFUPZBK-UHFFFAOYSA-N 3-[dimethyl-[3-(tetradecanoylamino)propyl]azaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound CCCCCCCCCCCCCC(=O)NCCC[N+](C)(C)CC(O)CS([O-])(=O)=O QDDVYXATFUPZBK-UHFFFAOYSA-N 0.000 claims description 5
• DDGPBVIAYDDWDH-UHFFFAOYSA-N 3-[dodecyl(dimethyl)azaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound CCCCCCCCCCCC[N+](C)(C)CC(O)CS([O-])(=O)=O DDGPBVIAYDDWDH-UHFFFAOYSA-N 0.000 claims description 5
• BIHLLDAYTURELE-UHFFFAOYSA-N 4-[[dodecyl(dimethyl)azaniumyl]methyl]benzoate Chemical compound CCCCCCCCCCCC[N+](C)(C)CC1=CC=C(C([O-])=O)C=C1 BIHLLDAYTURELE-UHFFFAOYSA-N 0.000 claims description 5
• VPWFGCUBMGNWNK-UHFFFAOYSA-N CCCCCCCCCCCCCCCCCC(=O)NCCC[N+](C)(C)CC(O)CS([O-])(=O)=O Chemical compound CCCCCCCCCCCCCCCCCC(=O)NCCC[N+](C)(C)CC(O)CS([O-])(=O)=O VPWFGCUBMGNWNK-UHFFFAOYSA-N 0.000 claims description 5
• 241001467553 Mycobacterium africanum Species 0.000 claims description 5
• 241000187919 Mycobacterium microti Species 0.000 claims description 5
• 241000187491 Mycobacterium nonchromogenicum Species 0.000 claims description 5
• 241000187490 Mycobacterium scrofulaceum Species 0.000 claims description 5
• 241000187489 Mycobacterium simiae Species 0.000 claims description 5
• 241000187480 Mycobacterium smegmatis Species 0.000 claims description 5
• 241000187917 Mycobacterium ulcerans Species 0.000 claims description 5
• QZHBDTJSHGSCNI-UHFFFAOYSA-N 3-[3-(hexadecanoylamino)propyl-dimethylazaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound CCCCCCCCCCCCCCCC(=O)NCCC[N+](C)(C)CC(O)CS([O-])(=O)=O QZHBDTJSHGSCNI-UHFFFAOYSA-N 0.000 claims description 4
• 235000010469 Glycine max Nutrition 0.000 claims description 4
• 241000187474 Mycobacterium asiaticum Species 0.000 claims description 4
• 241001455333 Mycobacterium fortuitum complex Species 0.000 claims description 4
• 241001147828 Mycobacterium haemophilum Species 0.000 claims description 4
• 241000908167 Mycobacterium lepraemurium Species 0.000 claims description 4
• 241000187481 Mycobacterium phlei Species 0.000 claims description 4
• 241000187476 Mycobacterium triviale Species 0.000 claims description 4
• 240000008042 Zea mays Species 0.000 claims description 4
• 125000004836 hexamethylene group Chemical group [H]C([H])([*:2])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[*:1] 0.000 claims description 4
• NMZCVTRJSNHYLV-UHFFFAOYSA-N 3-[(3-butoxy-2-hydroxypropyl)-dimethylazaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound CCCCOCC(O)C[N+](C)(C)CC(O)CS([O-])(=O)=O NMZCVTRJSNHYLV-UHFFFAOYSA-N 0.000 claims description 3
• DHMQDGOQFOQNFH-UHFFFAOYSA-M Aminoacetate Chemical compound NCC([O-])=O DHMQDGOQFOQNFH-UHFFFAOYSA-M 0.000 claims description 3
• DKONVCGLRMXHAG-UHFFFAOYSA-N CCCCCCCCCCCCCCCCCC[N+](C)(C)CS([O-])(=O)=O Chemical compound CCCCCCCCCCCCCCCCCC[N+](C)(C)CS([O-])(=O)=O DKONVCGLRMXHAG-UHFFFAOYSA-N 0.000 claims description 3
• 244000068988 Glycine max Species 0.000 claims description 3
• 241001182316 Linda Species 0.000 claims description 3
• 241000719287 Methylocystis rosea Species 0.000 claims description 3
• 241001532524 Mycobacterium duvalii Species 0.000 claims description 3
• 241001147830 Mycobacterium lufu Species 0.000 claims description 3
• 241000168058 Mycobacterium peregrinum Species 0.000 claims description 3
• 241001147832 Mycobacterium shimoidei Species 0.000 claims description 3
• 241000187644 Mycobacterium vaccae Species 0.000 claims description 3
• 244000021150 Orbignya martiana Species 0.000 claims description 3
• 235000014643 Orbignya martiana Nutrition 0.000 claims description 3
• PCISSFHSQFJBNX-UHFFFAOYSA-N sodium;2-[3-aminopropyl(dimethyl)azaniumyl]acetate Chemical compound [Na+].[O-]C(=O)C[N+](C)(C)CCCN PCISSFHSQFJBNX-UHFFFAOYSA-N 0.000 claims description 3
• FFJCNSLCJOQHKM-CLFAGFIQSA-N (z)-1-[(z)-octadec-9-enoxy]octadec-9-ene Chemical compound CCCCCCCC\C=C/CCCCCCCCOCCCCCCCC\C=C/CCCCCCCC FFJCNSLCJOQHKM-CLFAGFIQSA-N 0.000 claims description 2
• PXCQNTSWFRNXDI-UHFFFAOYSA-N 4-[dimethyl(octadecyl)azaniumyl]butanoate Chemical compound CCCCCCCCCCCCCCCCCC[N+](C)(C)CCCC([O-])=O PXCQNTSWFRNXDI-UHFFFAOYSA-N 0.000 claims 6
• MHFNLGMRJJKMSW-UHFFFAOYSA-N 2-[dimethyl(tetradecyl)azaniumyl]ethanesulfonate Chemical compound CCCCCCCCCCCCCC[N+](C)(C)CCS([O-])(=O)=O MHFNLGMRJJKMSW-UHFFFAOYSA-N 0.000 claims 4
• JMUAAHMVRDWJPQ-UHFFFAOYSA-N 2-[dodecyl(dimethyl)azaniumyl]ethanesulfonate Chemical compound CCCCCCCCCCCC[N+](C)(C)CCS([O-])(=O)=O JMUAAHMVRDWJPQ-UHFFFAOYSA-N 0.000 claims 4
• YLKDBVPMICCUJC-UHFFFAOYSA-N 2-[dodecyl(dimethyl)azaniumyl]ethyl hydrogen phosphate Chemical compound CCCCCCCCCCCC[N+](C)(C)CCOP(O)([O-])=O YLKDBVPMICCUJC-UHFFFAOYSA-N 0.000 claims 4
• SMWKMBMXOITBMO-UHFFFAOYSA-N 3-[dimethyl(octadecyl)azaniumyl]propanoate Chemical compound CCCCCCCCCCCCCCCCCC[N+](C)(C)CCC([O-])=O SMWKMBMXOITBMO-UHFFFAOYSA-N 0.000 claims 4
• JVQPXFSGBOXRHZ-UHFFFAOYSA-N 3-[dimethyl(pentadecyl)azaniumyl]propane-1-sulfonate Chemical compound CCCCCCCCCCCCCCC[N+](C)(C)CCCS([O-])(=O)=O JVQPXFSGBOXRHZ-UHFFFAOYSA-N 0.000 claims 4
• ZIPZOYYHNDZCHO-UHFFFAOYSA-N 3-[dimethyl(tetradecyl)azaniumyl]propanoate Chemical compound CCCCCCCCCCCCCC[N+](C)(C)CCC([O-])=O ZIPZOYYHNDZCHO-UHFFFAOYSA-N 0.000 claims 4
• QFTOCALGCQDGHN-UHFFFAOYSA-N 3-[dimethyl(tridecyl)azaniumyl]propanoate Chemical compound CCCCCCCCCCCCC[N+](C)(C)CCC([O-])=O QFTOCALGCQDGHN-UHFFFAOYSA-N 0.000 claims 4
• RNKPJTIIVCCOKI-UHFFFAOYSA-N 3-[dodecyl(dimethyl)azaniumyl]propanoate Chemical compound CCCCCCCCCCCC[N+](C)(C)CCC([O-])=O RNKPJTIIVCCOKI-UHFFFAOYSA-N 0.000 claims 4
• UQWLXAFTKJLLRL-UHFFFAOYSA-N 3-[hexadecyl(dimethyl)azaniumyl]propanoate Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)CCC([O-])=O UQWLXAFTKJLLRL-UHFFFAOYSA-N 0.000 claims 4
• QXGGNNVWTHXEGZ-UHFFFAOYSA-N 3-[hexadecyl(dimethyl)azaniumyl]propyl hydrogen phosphate Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)CCCOP(O)([O-])=O QXGGNNVWTHXEGZ-UHFFFAOYSA-N 0.000 claims 4
• ISZZVWZCCZYJAF-UHFFFAOYSA-N 4-[dimethyl(octadecyl)azaniumyl]butane-1-sulfonate Chemical compound CCCCCCCCCCCCCCCCCC[N+](C)(C)CCCCS([O-])(=O)=O ISZZVWZCCZYJAF-UHFFFAOYSA-N 0.000 claims 4
• XFHYQIWWBAVJDM-UHFFFAOYSA-N 4-[dimethyl(pentadecyl)azaniumyl]butanoate Chemical compound CCCCCCCCCCCCCCC[N+](C)(C)CCCC([O-])=O XFHYQIWWBAVJDM-UHFFFAOYSA-N 0.000 claims 4
• IDPWQVMSHKEORI-UHFFFAOYSA-N 4-[dimethyl(tetradecyl)azaniumyl]butanoate Chemical compound CCCCCCCCCCCCCC[N+](C)(C)CCCC([O-])=O IDPWQVMSHKEORI-UHFFFAOYSA-N 0.000 claims 4
• RMDLUIULVIQFSO-UHFFFAOYSA-N 4-[dimethyl(undecyl)azaniumyl]butanoate Chemical compound CCCCCCCCCCC[N+](C)(C)CCCC([O-])=O RMDLUIULVIQFSO-UHFFFAOYSA-N 0.000 claims 4
• UNIGAQKUMBZWBV-UHFFFAOYSA-N 4-[dodecyl(dimethyl)azaniumyl]butane-1-sulfonate Chemical compound CCCCCCCCCCCC[N+](C)(C)CCCCS([O-])(=O)=O UNIGAQKUMBZWBV-UHFFFAOYSA-N 0.000 claims 4
• RTCPDWIYJMYYQK-UHFFFAOYSA-N 4-[hexadecyl(dimethyl)azaniumyl]butane-1-sulfonate Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)CCCCS([O-])(=O)=O RTCPDWIYJMYYQK-UHFFFAOYSA-N 0.000 claims 4
• AIFIZFJELYASIF-UHFFFAOYSA-N 5-[dodecyl(dimethyl)azaniumyl]pentanoate Chemical compound CCCCCCCCCCCC[N+](C)(C)CCCCC([O-])=O AIFIZFJELYASIF-UHFFFAOYSA-N 0.000 claims 4
• PFFHMUGNLUIAGT-UHFFFAOYSA-N CCCCCCCCCCCCCCCCCC[N+](C)(C)CCOP([O-])(O)=O Chemical compound CCCCCCCCCCCCCCCCCC[N+](C)(C)CCOP([O-])(O)=O PFFHMUGNLUIAGT-UHFFFAOYSA-N 0.000 claims 4
• DQMIQLMZHJPHMN-UHFFFAOYSA-N CCCCCCCCCCCCCCCCC[N+](C)(C)CCOP([O-])(O)=O Chemical compound CCCCCCCCCCCCCCCCC[N+](C)(C)CCOP([O-])(O)=O DQMIQLMZHJPHMN-UHFFFAOYSA-N 0.000 claims 4
• LBFPQPSSYGGUDK-UHFFFAOYSA-N CCCCCCCCCCCCCCCC[N+](C)(C)CCCCOP([O-])(O)=O Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)CCCCOP([O-])(O)=O LBFPQPSSYGGUDK-UHFFFAOYSA-N 0.000 claims 4
• WUEVGFNFHQZBOP-UHFFFAOYSA-N CCCCCCCCCCCCCCCC[N+](C)(C)CCOP(O)([O-])=O Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)CCOP(O)([O-])=O WUEVGFNFHQZBOP-UHFFFAOYSA-N 0.000 claims 4
• WWYNZTMMQQKKCZ-UHFFFAOYSA-N CCCCCCCCCCCCCC[N+](C)(C)CCOP([O-])(O)=O Chemical compound CCCCCCCCCCCCCC[N+](C)(C)CCOP([O-])(O)=O WWYNZTMMQQKKCZ-UHFFFAOYSA-N 0.000 claims 4
• DVWYPEFHZKJVAF-UHFFFAOYSA-N C[N+](C)(CCCCS([O-])(=O)=O)CCCNCCCCCCCCCCCCCCCC=O Chemical compound C[N+](C)(CCCCS([O-])(=O)=O)CCCNCCCCCCCCCCCCCCCC=O DVWYPEFHZKJVAF-UHFFFAOYSA-N 0.000 claims 4
• JWRZDDGWGBUEEX-UHFFFAOYSA-N CCCCCCCCCCCCCCCC[N+](C)(C)CCS([O-])(=O)=O Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)CCS([O-])(=O)=O JWRZDDGWGBUEEX-UHFFFAOYSA-N 0.000 claims 3
• IZWSFJTYBVKZNK-UHFFFAOYSA-O N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonic acid Chemical compound CCCCCCCCCCCC[N+](C)(C)CCCS(O)(=O)=O IZWSFJTYBVKZNK-UHFFFAOYSA-O 0.000 claims 3
• 125000003258 trimethylene group Chemical group [H]C([H])([*:2])C([H])([H])C([H])([H])[*:1] 0.000 claims 3
• QCJUEFDPQHRCMX-UHFFFAOYSA-N 1-[hexadecyl(dimethyl)azaniumyl]propane-2-sulfonate Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)CC(C)S([O-])(=O)=O QCJUEFDPQHRCMX-UHFFFAOYSA-N 0.000 claims 2
• JKPNSPBJYYUEBN-UHFFFAOYSA-N 2-[2-hydroxyhexadecyl(dimethyl)azaniumyl]ethyl hydrogen phosphate Chemical compound CCCCCCCCCCCCCCC(O)C[N+](C)(C)CCOP(O)([O-])=O JKPNSPBJYYUEBN-UHFFFAOYSA-N 0.000 claims 2
• WKVUHAXYEKLZRI-UHFFFAOYSA-N 2-[2-hydroxytetradecyl(dimethyl)azaniumyl]ethyl hydrogen phosphate Chemical compound CCCCCCCCCCCCC(O)C[N+](C)(C)CCOP(O)([O-])=O WKVUHAXYEKLZRI-UHFFFAOYSA-N 0.000 claims 2
• LJXQDBPJFICXJU-UHFFFAOYSA-N 2-[3-aminopropyl(dimethyl)azaniumyl]acetate Chemical compound [O-]C(=O)C[N+](C)(C)CCCN LJXQDBPJFICXJU-UHFFFAOYSA-N 0.000 claims 2
• QVFURWHQRRYPAM-UHFFFAOYSA-N 3-[dimethyl(octadecyl)azaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound CCCCCCCCCCCCCCCCCC[N+](C)(C)CC(O)CS([O-])(=O)=O QVFURWHQRRYPAM-UHFFFAOYSA-N 0.000 claims 2
• BYLCDXOXSNGMEY-UHFFFAOYSA-N 4-[[dodecyl(dimethyl)azaniumyl]methyl]benzenesulfonate Chemical compound CCCCCCCCCCCC[N+](C)(C)CC1=CC=C(C=C1)S(=O)(=O)[O-] BYLCDXOXSNGMEY-UHFFFAOYSA-N 0.000 claims 2
• BUFVMHYCMFITOB-UHFFFAOYSA-N 6-[dodecyl(dimethyl)azaniumyl]hexanoate Chemical compound CCCCCCCCCCCC[N+](C)(C)CCCCCC([O-])=O BUFVMHYCMFITOB-UHFFFAOYSA-N 0.000 claims 2
• CVXJASFUDGDJPK-UHFFFAOYSA-N CCCCCCCCC=CCCCCCCCC[N+](C)(C)CCOP([O-])(O)=O Chemical compound CCCCCCCCC=CCCCCCCCC[N+](C)(C)CCOP([O-])(O)=O CVXJASFUDGDJPK-UHFFFAOYSA-N 0.000 claims 2
• CTTXATDJXVJNLW-UHFFFAOYSA-N CCCCCCCCCCC(C[N+](C)(C)CCOP([O-])(O)=O)O Chemical compound CCCCCCCCCCC(C[N+](C)(C)CCOP([O-])(O)=O)O CTTXATDJXVJNLW-UHFFFAOYSA-N 0.000 claims 2
• HIUSIOAYZWYBFI-UHFFFAOYSA-N CCCCCCCCCCCC(NCCC[N+](C)(C)CC(C=C1)=CC=C1S([O-])(=O)=O)=O Chemical compound CCCCCCCCCCCC(NCCC[N+](C)(C)CC(C=C1)=CC=C1S([O-])(=O)=O)=O HIUSIOAYZWYBFI-UHFFFAOYSA-N 0.000 claims 2
• BHRCNJVUAZKCMO-UHFFFAOYSA-N CCCCCCCCCCCC(NCCC[N+](C)(C)CC(COP([O-])(O)=O)O)=O Chemical compound CCCCCCCCCCCC(NCCC[N+](C)(C)CC(COP([O-])(O)=O)O)=O BHRCNJVUAZKCMO-UHFFFAOYSA-N 0.000 claims 2
• WUFUEDSZDGCOQR-UHFFFAOYSA-N CCCCCCCCCCCCC(C)[N+](C)(C)CC(CS([O-])(=O)=O)O Chemical compound CCCCCCCCCCCCC(C)[N+](C)(C)CC(CS([O-])(=O)=O)O WUFUEDSZDGCOQR-UHFFFAOYSA-N 0.000 claims 2
• VWXSSKNLEIHUDI-UHFFFAOYSA-N CCCCCCCCCCCCCCCC(NCCC[N+](C)(C)CC(C)S([O-])(=O)=O)=O Chemical compound CCCCCCCCCCCCCCCC(NCCC[N+](C)(C)CC(C)S([O-])(=O)=O)=O VWXSSKNLEIHUDI-UHFFFAOYSA-N 0.000 claims 2
• CGQBFEIRTXXSSV-UHFFFAOYSA-N CCCCCCCCCCCCCCCC(NCCC[N+](C)(C)CCS([O-])(=O)=O)=O Chemical compound CCCCCCCCCCCCCCCC(NCCC[N+](C)(C)CCS([O-])(=O)=O)=O CGQBFEIRTXXSSV-UHFFFAOYSA-N 0.000 claims 2
• DWMPVQSBCJUISB-UHFFFAOYSA-N CCCCCCCCCCCCCCCCC=C[N+](C)(C)CCOP([O-])(O)=O Chemical compound CCCCCCCCCCCCCCCCC=C[N+](C)(C)CCOP([O-])(O)=O DWMPVQSBCJUISB-UHFFFAOYSA-N 0.000 claims 2
• UBQJVRGTFBBSJR-UHFFFAOYSA-N CCCCCCCCCCCCCCCCCC(NCCC[N+](C)(C)CC(COP([O-])(O)=O)O)=O Chemical compound CCCCCCCCCCCCCCCCCC(NCCC[N+](C)(C)CC(COP([O-])(O)=O)O)=O UBQJVRGTFBBSJR-UHFFFAOYSA-N 0.000 claims 2
• ZENBCBBUYOFJTR-UHFFFAOYSA-N CCCCCCCCCCCCCCCCCC[N+](C)(C)CC(C=C1)=CC=C1S([O-])(=O)=O Chemical compound CCCCCCCCCCCCCCCCCC[N+](C)(C)CC(C=C1)=CC=C1S([O-])(=O)=O ZENBCBBUYOFJTR-UHFFFAOYSA-N 0.000 claims 2
• ZMPFSIHBFFKFHO-UHFFFAOYSA-N CCCCCCCCCCCCCCCC[N+](C)(C)CC(C=C1)=CC=C1C([O-])=O Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)CC(C=C1)=CC=C1C([O-])=O ZMPFSIHBFFKFHO-UHFFFAOYSA-N 0.000 claims 2
• OOUOJNMZALLMLR-UHFFFAOYSA-N CCCCCCCCCCCCCCCC[N+](C)(C)CC(C=C1)=CC=C1S([O-])(=O)=O Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)CC(C=C1)=CC=C1S([O-])(=O)=O OOUOJNMZALLMLR-UHFFFAOYSA-N 0.000 claims 2
• MVPLRIMUTVTUNX-UHFFFAOYSA-N CCCCCCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1C([O-])=O Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1C([O-])=O MVPLRIMUTVTUNX-UHFFFAOYSA-N 0.000 claims 2
• OPFPIEYWBGSSJQ-UHFFFAOYSA-N CCCCCCCCCCCCCCCC[N+](C)(C)CCCCCCS([O-])(=O)=O Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)CCCCCCS([O-])(=O)=O OPFPIEYWBGSSJQ-UHFFFAOYSA-N 0.000 claims 2
• MERYHQJJYUEDCK-UHFFFAOYSA-N CCCCCCCCCCCCCCCC[N+](C)(C)CS([O-])(=O)=O Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)CS([O-])(=O)=O MERYHQJJYUEDCK-UHFFFAOYSA-N 0.000 claims 2
• RMXDDCCLDILMRN-UHFFFAOYSA-N CCCCCCCCCCCCCCCC[N+](C)(CC)CCOP([O-])(O)=O Chemical compound CCCCCCCCCCCCCCCC[N+](C)(CC)CCOP([O-])(O)=O RMXDDCCLDILMRN-UHFFFAOYSA-N 0.000 claims 2
• YWYAREZZYSNJNG-UHFFFAOYSA-N CCCCCCCCCCCCCCCC[N+](CC)(CC)CCOP([O-])(O)=O Chemical compound CCCCCCCCCCCCCCCC[N+](CC)(CC)CCOP([O-])(O)=O YWYAREZZYSNJNG-UHFFFAOYSA-N 0.000 claims 2
• KLWQTQFYVQICFL-UHFFFAOYSA-N CCCCCCCCCCCCCCCC[N+](CC)(CCC)CCOP([O-])(O)=O Chemical compound CCCCCCCCCCCCCCCC[N+](CC)(CCC)CCOP([O-])(O)=O KLWQTQFYVQICFL-UHFFFAOYSA-N 0.000 claims 2
• IAPONILPKVLOTH-UHFFFAOYSA-N CCCCCCCCCCCCCCCC[N+](CC)(CCCC)CCOP([O-])(O)=O Chemical compound CCCCCCCCCCCCCCCC[N+](CC)(CCCC)CCOP([O-])(O)=O IAPONILPKVLOTH-UHFFFAOYSA-N 0.000 claims 2
• YUTCSFVQBBHZGL-UHFFFAOYSA-N CCCCCCCCCCCCCCCC[N+](CCC)(CCC)CCOP([O-])(O)=O Chemical compound CCCCCCCCCCCCCCCC[N+](CCC)(CCC)CCOP([O-])(O)=O YUTCSFVQBBHZGL-UHFFFAOYSA-N 0.000 claims 2
• UMQJOFQHROFINA-UHFFFAOYSA-N CCCCCCCCCCCCCCCC[N+](CCCC)(CCCC)CCOP([O-])(O)=O Chemical compound CCCCCCCCCCCCCCCC[N+](CCCC)(CCCC)CCOP([O-])(O)=O UMQJOFQHROFINA-UHFFFAOYSA-N 0.000 claims 2
• WNAYWULQAXJNCW-UHFFFAOYSA-N CCCCCCCCCCCCCCC[N+](C)(C)CC(CS([O-])(=O)=O)O Chemical compound CCCCCCCCCCCCCCC[N+](C)(C)CC(CS([O-])(=O)=O)O WNAYWULQAXJNCW-UHFFFAOYSA-N 0.000 claims 2
• NZPMSOROCWLLQW-UHFFFAOYSA-N CCCCCCCCCCCCCCOCC(C[N+](C)(C)CCOP([O-])(O)=O)O Chemical compound CCCCCCCCCCCCCCOCC(C[N+](C)(C)CCOP([O-])(O)=O)O NZPMSOROCWLLQW-UHFFFAOYSA-N 0.000 claims 2
• MJIJFIAQTGAHQT-UHFFFAOYSA-N CCCCCCCCCCCCCC[N+](C)(C)CC(C)S([O-])(=O)=O Chemical compound CCCCCCCCCCCCCC[N+](C)(C)CC(C)S([O-])(=O)=O MJIJFIAQTGAHQT-UHFFFAOYSA-N 0.000 claims 2
• BBMFZSYNIWICEQ-UHFFFAOYSA-N CCCCCCCCCCCCCC[N+](C)(C)CC(C=C1)=CC=C1S([O-])(=O)=O Chemical compound CCCCCCCCCCCCCC[N+](C)(C)CC(C=C1)=CC=C1S([O-])(=O)=O BBMFZSYNIWICEQ-UHFFFAOYSA-N 0.000 claims 2
• MKVGHZQZSOASJO-UHFFFAOYSA-N CCCCCCCCCCCCOCC(C[N+](C)(C)CCOP([O-])(O)=O)O Chemical compound CCCCCCCCCCCCOCC(C[N+](C)(C)CCOP([O-])(O)=O)O MKVGHZQZSOASJO-UHFFFAOYSA-N 0.000 claims 2
• XCALROFBULULBW-UHFFFAOYSA-N CCCCCCCCCCCC[N+](C)(C)C(C)CC(C)(C)S([O-])(=O)=O Chemical compound CCCCCCCCCCCC[N+](C)(C)C(C)CC(C)(C)S([O-])(=O)=O XCALROFBULULBW-UHFFFAOYSA-N 0.000 claims 2
• PKLQMVJLUQWPQC-UHFFFAOYSA-N CCCCCCCCCCCC[N+](C)(C)CC(C)S([O-])(=O)=O Chemical compound CCCCCCCCCCCC[N+](C)(C)CC(C)S([O-])(=O)=O PKLQMVJLUQWPQC-UHFFFAOYSA-N 0.000 claims 2
• WYNTUHRMCWSFRR-UHFFFAOYSA-N CCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1C([O-])=O Chemical compound CCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1C([O-])=O WYNTUHRMCWSFRR-UHFFFAOYSA-N 0.000 claims 2
• FMQNBKMHIGSQHG-UHFFFAOYSA-N CCCCCCCCCCC[N+](C)(C)CCOP([O-])(O)=O Chemical compound CCCCCCCCCCC[N+](C)(C)CCOP([O-])(O)=O FMQNBKMHIGSQHG-UHFFFAOYSA-N 0.000 claims 2
• PMZVUMZQFLQBOJ-UHFFFAOYSA-N CCCCCCCCCCOCC(C[N+](C)(C)CCOP([O-])(O)=O)O Chemical compound CCCCCCCCCCOCC(C[N+](C)(C)CCOP([O-])(O)=O)O PMZVUMZQFLQBOJ-UHFFFAOYSA-N 0.000 claims 2
• YYNUZHZJJYDWIC-UHFFFAOYSA-N CCCCCCCCCC[N+](C)(C)CCOP([O-])(O)=O Chemical compound CCCCCCCCCC[N+](C)(C)CCOP([O-])(O)=O YYNUZHZJJYDWIC-UHFFFAOYSA-N 0.000 claims 2
• RVNKUVTYQJBDBB-UHFFFAOYSA-N C[N+](CCCCCCCCCC)(C)CC(CS(=O)(=O)[O-])O Chemical compound C[N+](CCCCCCCCCC)(C)CC(CS(=O)(=O)[O-])O RVNKUVTYQJBDBB-UHFFFAOYSA-N 0.000 claims 2
• 241000187495 Mycobacterium terrae Species 0.000 claims 2
• 150000005215 alkyl ethers Chemical class 0.000 claims 2
• YKZVNPYBDHKUSV-UHFFFAOYSA-N dodecyl-dipropyl-(3-sulfopropyl)azanium;hydroxide Chemical compound [OH-].CCCCCCCCCCCC[N+](CCC)(CCC)CCCS(O)(=O)=O YKZVNPYBDHKUSV-UHFFFAOYSA-N 0.000 claims 2
• 125000000383 tetramethylene group Chemical group [H]C([H])([*:1])C([H])([H])C([H])([H])C([H])([H])[*:2] 0.000 claims 2
• ZMXWTYDZWPGTOM-UHFFFAOYSA-N 2-[3-(12-hydroxyoctadec-9-enoylamino)propyl-dimethylazaniumyl]acetate Chemical compound CCCCCCC(O)CC=CCCCCCCCC(=O)NCCC[N+](C)(C)CC([O-])=O ZMXWTYDZWPGTOM-UHFFFAOYSA-N 0.000 claims 1
• AMRBZKOCOOPYNY-UHFFFAOYSA-N 2-[dimethyl(octadec-9-enyl)azaniumyl]acetate Chemical compound CCCCCCCCC=CCCCCCCCC[N+](C)(C)CC([O-])=O AMRBZKOCOOPYNY-UHFFFAOYSA-N 0.000 claims 1
• HOOKUTCXRFSDNB-UHFFFAOYSA-N 6-[hexadecyl(dimethyl)azaniumyl]hexanoate Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)CCCCCC([O-])=O HOOKUTCXRFSDNB-UHFFFAOYSA-N 0.000 claims 1
• KOIDLXBIUVRLHP-UHFFFAOYSA-N 7-[dodecyl(dimethyl)azaniumyl]heptanoate Chemical compound CCCCCCCCCCCC[N+](C)(C)CCCCCCC([O-])=O KOIDLXBIUVRLHP-UHFFFAOYSA-N 0.000 claims 1
• 241000606560 Avibacterium avium Species 0.000 claims 1
• RORXGROCBUTXEK-UHFFFAOYSA-N CCCCCCCCCCCCCC(NCCC[N+](C)(C)CC(C=C1)=CC=C1S([O-])(=O)=O)=O Chemical compound CCCCCCCCCCCCCC(NCCC[N+](C)(C)CC(C=C1)=CC=C1S([O-])(=O)=O)=O RORXGROCBUTXEK-UHFFFAOYSA-N 0.000 claims 1
• KNYFNBMFBNKMFY-UHFFFAOYSA-N CCCCCCCCCCCCCCCCCC(NCCC[N+](C)(C)CC(C=C1)=CC=C1S([O-])(=O)=O)=O Chemical compound CCCCCCCCCCCCCCCCCC(NCCC[N+](C)(C)CC(C=C1)=CC=C1S([O-])(=O)=O)=O KNYFNBMFBNKMFY-UHFFFAOYSA-N 0.000 claims 1
• SPXMUHFDUIXKBX-UHFFFAOYSA-N CCCCCCCCCCCCCCCC[N+](C)(C)CCCCCCOP([O-])(O)=O Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)CCCCCCOP([O-])(O)=O SPXMUHFDUIXKBX-UHFFFAOYSA-N 0.000 claims 1
• ZNDACXZEOXVFNB-UHFFFAOYSA-N [dodecyl(dimethyl)azaniumyl]methanesulfonate Chemical compound CCCCCCCCCCCC[N+](C)(C)CS([O-])(=O)=O ZNDACXZEOXVFNB-UHFFFAOYSA-N 0.000 claims 1
• 230000003321 amplification Effects 0.000 abstract description 204
• 238000003199 nucleic acid amplification method Methods 0.000 abstract description 204
• 239000007788 liquid Substances 0.000 abstract description 9
• 239000007787 solid Substances 0.000 abstract description 4
• 239000000523 sample Substances 0.000 description 213
• KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 description 139
• UYIQQMNVTFFUHZ-UHFFFAOYSA-M [2-(dimethylcarbamoyloxy)-5-propan-2-ylphenyl]-trimethylazanium;iodide Chemical compound [I-].CC(C)C1=CC=C(OC(=O)N(C)C)C([N+](C)(C)C)=C1 UYIQQMNVTFFUHZ-UHFFFAOYSA-M 0.000 description 130
• HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 130
• 238000003752 polymerase chain reaction Methods 0.000 description 94
• 230000000694 effects Effects 0.000 description 83
• 241000187479 Mycobacterium tuberculosis Species 0.000 description 81
• 239000000243 solution Substances 0.000 description 76
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 72
• 238000005119 centrifugation Methods 0.000 description 71
• 239000013049 sediment Substances 0.000 description 71
• 210000004027 cell Anatomy 0.000 description 70
• 239000000203 mixture Substances 0.000 description 67
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 62
• 239000003921 oil Substances 0.000 description 59
• 235000019198 oils Nutrition 0.000 description 59
• 238000007872 degassing Methods 0.000 description 51
• 239000000693 micelle Substances 0.000 description 45
• 239000000872 buffer Substances 0.000 description 44
• PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 description 42
• 241001502334 Mycobacterium avium complex bacterium Species 0.000 description 42
• 238000002474 experimental method Methods 0.000 description 42
• 210000002421 cell wall Anatomy 0.000 description 40
• 230000006399 behavior Effects 0.000 description 39
• 230000001965 increasing effect Effects 0.000 description 39
• 150000002632 lipids Chemical class 0.000 description 39
• 238000003556 assay Methods 0.000 description 38
• 230000006870 function Effects 0.000 description 38
• VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 36
• 239000011534 wash buffer Substances 0.000 description 35
• 150000001450 anions Chemical class 0.000 description 33
• 238000004458 analytical method Methods 0.000 description 32
• 230000015572 biosynthetic process Effects 0.000 description 32
• 230000012010 growth Effects 0.000 description 31
• 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 31
• 229920000053 polysorbate 80 Polymers 0.000 description 31
• 239000011780 sodium chloride Substances 0.000 description 31
• 230000002776 aggregation Effects 0.000 description 28
• 238000004220 aggregation Methods 0.000 description 28
• 239000003792 electrolyte Substances 0.000 description 28
• ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 28
• 150000001875 compounds Chemical class 0.000 description 27
• 238000009396 hybridization Methods 0.000 description 27
• 239000003112 inhibitor Substances 0.000 description 26
• 238000000605 extraction Methods 0.000 description 25
• DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 23
• 201000010099 disease Diseases 0.000 description 23
• 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 23
• 239000008188 pellet Substances 0.000 description 23
• 150000007513 acids Chemical class 0.000 description 22
• 150000001768 cations Chemical class 0.000 description 22
• 239000000126 substance Substances 0.000 description 22
• WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 21
• LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 21
• QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 21
• 239000005642 Oleic acid Substances 0.000 description 21
• ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 21
• 230000002209 hydrophobic effect Effects 0.000 description 21
• QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 21
• IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Substances N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 21
• 239000011550 stock solution Substances 0.000 description 21
• 230000001580 bacterial effect Effects 0.000 description 20
• 230000035945 sensitivity Effects 0.000 description 20
• 239000012139 lysis buffer Substances 0.000 description 19
• 230000004048 modification Effects 0.000 description 19
• 238000012986 modification Methods 0.000 description 19
• QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 19
• MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 18
• 150000001412 amines Chemical group 0.000 description 18
• 230000036541 health Effects 0.000 description 18
• 230000002441 reversible effect Effects 0.000 description 18
• QAOWNCQODCNURD-UHFFFAOYSA-L sulfate group Chemical group S(=O)(=O)([O-])[O-] QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 18
• 239000004094 surface-active agent Substances 0.000 description 18
• ONYHQNURMVNRJZ-QXMHVHEDSA-N 3-[3-[[(Z)-docos-13-enoyl]amino]propyl-dimethylazaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound CCCCCCCC\C=C/CCCCCCCCCCCC(=O)NCCC[N+](C)(C)CC(O)CS([O-])(=O)=O ONYHQNURMVNRJZ-QXMHVHEDSA-N 0.000 description 17
• KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 17
• 239000000463 material Substances 0.000 description 17
• 150000007523 nucleic acids Chemical class 0.000 description 17
• 239000012071 phase Substances 0.000 description 17
• 108020004414 DNA Proteins 0.000 description 16
• 108020004707 nucleic acids Proteins 0.000 description 16
• 102000039446 nucleic acids Human genes 0.000 description 16
• 238000005185 salting out Methods 0.000 description 16
• QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 15
• 206010036790 Productive cough Diseases 0.000 description 15
• 230000008901 benefit Effects 0.000 description 15
• 230000001419 dependent effect Effects 0.000 description 15
• 230000005180 public health Effects 0.000 description 15
• 210000003802 sputum Anatomy 0.000 description 15
• 208000024794 sputum Diseases 0.000 description 15
• 238000003786 synthesis reaction Methods 0.000 description 15
• 238000006243 chemical reaction Methods 0.000 description 14
• 238000005516 engineering process Methods 0.000 description 14
• IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 14
• NWZBFJYXRGSRGD-UHFFFAOYSA-M sodium;octadecyl sulfate Chemical compound [Na+].CCCCCCCCCCCCCCCCCCOS([O-])(=O)=O NWZBFJYXRGSRGD-UHFFFAOYSA-M 0.000 description 14
• 238000012360 testing method Methods 0.000 description 14
• 241000894006 Bacteria Species 0.000 description 13
• QYOVMAREBTZLBT-KTKRTIGZSA-N CCCCCCCC\C=C/CCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO Chemical compound CCCCCCCC\C=C/CCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO QYOVMAREBTZLBT-KTKRTIGZSA-N 0.000 description 13
• 239000003153 chemical reaction reagent Substances 0.000 description 13
• 230000005764 inhibitory process Effects 0.000 description 13
• 239000000047 product Substances 0.000 description 13
• 108090000623 proteins and genes Proteins 0.000 description 13
• 229910019142 PO4 Inorganic materials 0.000 description 12
• WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 12
• 229920004890 Triton X-100 Polymers 0.000 description 12
• 238000011534 incubation Methods 0.000 description 12
• 208000015181 infectious disease Diseases 0.000 description 12
• 239000002609 medium Substances 0.000 description 12
• 235000021317 phosphate Nutrition 0.000 description 12
• 239000010452 phosphate Substances 0.000 description 12
• 239000006228 supernatant Substances 0.000 description 12
• SZEMGTQCPRNXEG-UHFFFAOYSA-M trimethyl(octadecyl)azanium;bromide Chemical compound [Br-].CCCCCCCCCCCCCCCCCC[N+](C)(C)C SZEMGTQCPRNXEG-UHFFFAOYSA-M 0.000 description 12
• 239000003240 coconut oil Substances 0.000 description 11
• 235000019864 coconut oil Nutrition 0.000 description 11
• 239000000084 colloidal system Substances 0.000 description 11
• 230000000875 corresponding effect Effects 0.000 description 11
• 230000006872 improvement Effects 0.000 description 11
• 239000013641 positive control Substances 0.000 description 11
• 239000002904 solvent Substances 0.000 description 11
• 229920001213 Polysorbate 20 Polymers 0.000 description 10
• 108010013381 Porins Proteins 0.000 description 10
• 102000017033 Porins Human genes 0.000 description 10
• 239000013504 Triton X-100 Substances 0.000 description 10
• 230000009089 cytolysis Effects 0.000 description 10
• 239000007789 gas Substances 0.000 description 10
• 150000002500 ions Chemical class 0.000 description 10
• 238000004519 manufacturing process Methods 0.000 description 10
• 239000013642 negative control Substances 0.000 description 10
• 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 10
• 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 10
• NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 10
• 230000002829 reductive effect Effects 0.000 description 10
• 238000005063 solubilization Methods 0.000 description 10
• 230000007928 solubilization Effects 0.000 description 10
• SFVFIFLLYFPGHH-UHFFFAOYSA-M stearalkonium chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 SFVFIFLLYFPGHH-UHFFFAOYSA-M 0.000 description 10
• 239000008223 sterile water Substances 0.000 description 10
• 241000186359 Mycobacterium Species 0.000 description 9
• 230000035508 accumulation Effects 0.000 description 9
• 238000009825 accumulation Methods 0.000 description 9
• 238000009835 boiling Methods 0.000 description 9
• 230000007423 decrease Effects 0.000 description 9
• 238000013461 design Methods 0.000 description 9
• 235000014113 dietary fatty acids Nutrition 0.000 description 9
• 239000000194 fatty acid Substances 0.000 description 9
• 229930195729 fatty acid Natural products 0.000 description 9
• 150000004665 fatty acids Chemical class 0.000 description 9
• 238000010348 incorporation Methods 0.000 description 9
• 230000007246 mechanism Effects 0.000 description 9
• 238000002156 mixing Methods 0.000 description 9
• 239000011541 reaction mixture Substances 0.000 description 9
• UMCMPZBLKLEWAF-BCTGSCMUSA-N 3-[(3-cholamidopropyl)dimethylammonio]propane-1-sulfonate Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)C)[C@@]2(C)[C@@H](O)C1 UMCMPZBLKLEWAF-BCTGSCMUSA-N 0.000 description 8
• 229920001817 Agar Polymers 0.000 description 8
• HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 8
• OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 8
• 241001465754 Metazoa Species 0.000 description 8
• 241000276498 Pollachius virens Species 0.000 description 8
• 239000008272 agar Substances 0.000 description 8
• 230000003385 bacteriostatic effect Effects 0.000 description 8
• 238000002955 isolation Methods 0.000 description 8
• 125000005647 linker group Chemical group 0.000 description 8
• OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 8
• 230000036961 partial effect Effects 0.000 description 8
• NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 8
• 239000013612 plasmid Substances 0.000 description 8
• 229920000056 polyoxyethylene ether Polymers 0.000 description 8
• 238000009849 vacuum degassing Methods 0.000 description 8
• 241000283690 Bos taurus Species 0.000 description 7
• RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 7
• 206010062207 Mycobacterial infection Diseases 0.000 description 7
• 150000001413 amino acids Chemical class 0.000 description 7
• 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 description 7
• 230000001010 compromised effect Effects 0.000 description 7
• 235000020778 linoleic acid Nutrition 0.000 description 7
• 208000027531 mycobacterial infectious disease Diseases 0.000 description 7
• 238000001556 precipitation Methods 0.000 description 7
• 102000004169 proteins and genes Human genes 0.000 description 7
• 238000000746 purification Methods 0.000 description 7
• 230000035899 viability Effects 0.000 description 7
• 208000030507 AIDS Diseases 0.000 description 6
• 108010067770 Endopeptidase K Proteins 0.000 description 6
• LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
• 235000021314 Palmitic acid Nutrition 0.000 description 6
• 239000002202 Polyethylene glycol Substances 0.000 description 6
• NWGKJDSIEKMTRX-AAZCQSIUSA-N Sorbitan monooleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O NWGKJDSIEKMTRX-AAZCQSIUSA-N 0.000 description 6
• 239000007983 Tris buffer Substances 0.000 description 6
• 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 6
• 230000005484 gravity Effects 0.000 description 6
• 238000001727 in vivo Methods 0.000 description 6
• WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 6
• 125000001117 oleyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])/C([H])=C([H])\C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 6
• 235000006408 oxalic acid Nutrition 0.000 description 6
• 229920001223 polyethylene glycol Polymers 0.000 description 6
• 229940051841 polyoxyethylene ether Drugs 0.000 description 6
• 239000001103 potassium chloride Substances 0.000 description 6
• 235000011164 potassium chloride Nutrition 0.000 description 6
• 239000002244 precipitate Substances 0.000 description 6
• 230000004044 response Effects 0.000 description 6
• 238000000926 separation method Methods 0.000 description 6
• 239000011734 sodium Substances 0.000 description 6
• BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 6
• LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 6
• 108020004465 16S ribosomal RNA Proteins 0.000 description 5
• COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 5
• 235000021355 Stearic acid Nutrition 0.000 description 5
• 208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 description 5
• 206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 5
• 102100037111 Uracil-DNA glycosylase Human genes 0.000 description 5
• 230000009471 action Effects 0.000 description 5
• 239000012472 biological sample Substances 0.000 description 5
• 210000004369 blood Anatomy 0.000 description 5
• 239000008280 blood Substances 0.000 description 5
• 125000002091 cationic group Chemical group 0.000 description 5
• 238000007821 culture assay Methods 0.000 description 5
• 238000010790 dilution Methods 0.000 description 5
• 239000012895 dilution Substances 0.000 description 5
• NAPSCFZYZVSQHF-UHFFFAOYSA-N dimantine Chemical compound CCCCCCCCCCCCCCCCCCN(C)C NAPSCFZYZVSQHF-UHFFFAOYSA-N 0.000 description 5
• 238000009826 distribution Methods 0.000 description 5
• POULHZVOKOAJMA-UHFFFAOYSA-N dodecanoic acid Chemical compound CCCCCCCCCCCC(O)=O POULHZVOKOAJMA-UHFFFAOYSA-N 0.000 description 5
• XJWSAJYUBXQQDR-UHFFFAOYSA-M dodecyltrimethylammonium bromide Chemical compound [Br-].CCCCCCCCCCCC[N+](C)(C)C XJWSAJYUBXQQDR-UHFFFAOYSA-M 0.000 description 5
• 239000003814 drug Substances 0.000 description 5
• 210000003608 fece Anatomy 0.000 description 5
• 238000001914 filtration Methods 0.000 description 5
• 230000033001 locomotion Effects 0.000 description 5
• 238000002844 melting Methods 0.000 description 5
• 230000008018 melting Effects 0.000 description 5
• 239000002773 nucleotide Substances 0.000 description 5
• 125000003729 nucleotide group Chemical group 0.000 description 5
• QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 5
• OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 5
• 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 5
• COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 5
• 229920000136 polysorbate Polymers 0.000 description 5
• 239000011347 resin Substances 0.000 description 5
• 229920005989 resin Polymers 0.000 description 5
• 238000012552 review Methods 0.000 description 5
• 239000008117 stearic acid Substances 0.000 description 5
• 239000012134 supernatant fraction Substances 0.000 description 5
• 238000009489 vacuum treatment Methods 0.000 description 5
• 238000009736 wetting Methods 0.000 description 5
• 239000010497 wheat germ oil Substances 0.000 description 5
• JWNWCEAWZGLYTE-UHFFFAOYSA-N 2-(trimethylazaniumyl)butanoate Chemical compound CCC(C([O-])=O)[N+](C)(C)C JWNWCEAWZGLYTE-UHFFFAOYSA-N 0.000 description 4
• HNUQMTZUNUBOLQ-UHFFFAOYSA-N 2-[2-[2-[2-[2-[2-[2-[2-[2-(2-octadecoxyethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethanol Chemical compound CCCCCCCCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO HNUQMTZUNUBOLQ-UHFFFAOYSA-N 0.000 description 4
• XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 4
• 241000186427 Cutibacterium acnes Species 0.000 description 4
• KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
• 229930186217 Glycolipid Natural products 0.000 description 4
• 241000725303 Human immunodeficiency virus Species 0.000 description 4
• 206010024229 Leprosy Diseases 0.000 description 4
• TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
• ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 4
• VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
• 239000003957 anion exchange resin Substances 0.000 description 4
• 230000000844 anti-bacterial effect Effects 0.000 description 4
• 230000000845 anti-microbial effect Effects 0.000 description 4
• 239000000427 antigen Substances 0.000 description 4
• 108091007433 antigens Proteins 0.000 description 4
• 102000036639 antigens Human genes 0.000 description 4
• OCBHHZMJRVXXQK-UHFFFAOYSA-M benzyl-dimethyl-tetradecylazanium;chloride Chemical compound [Cl-].CCCCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 OCBHHZMJRVXXQK-UHFFFAOYSA-M 0.000 description 4
• 239000013060 biological fluid Substances 0.000 description 4
• 239000000828 canola oil Substances 0.000 description 4
• 235000019519 canola oil Nutrition 0.000 description 4
• 239000004359 castor oil Substances 0.000 description 4
• 235000019438 castor oil Nutrition 0.000 description 4
• 210000001175 cerebrospinal fluid Anatomy 0.000 description 4
• 239000013078 crystal Substances 0.000 description 4
• 125000002147 dimethylamino group Chemical group [H]C([H])([H])N(*)C([H])([H])[H] 0.000 description 4
• 230000002255 enzymatic effect Effects 0.000 description 4
• 239000012530 fluid Substances 0.000 description 4
• ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 4
• 125000002887 hydroxy group Chemical group [H]O* 0.000 description 4
• VKOBVWXKNCXXDE-UHFFFAOYSA-N icosanoic acid Chemical compound CCCCCCCCCCCCCCCCCCCC(O)=O VKOBVWXKNCXXDE-UHFFFAOYSA-N 0.000 description 4
• PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 4
• 238000007834 ligase chain reaction Methods 0.000 description 4
• KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 description 4
• 210000004072 lung Anatomy 0.000 description 4
• 238000010943 off-gassing Methods 0.000 description 4
• 230000008520 organization Effects 0.000 description 4
• 238000005191 phase separation Methods 0.000 description 4
• ACVYVLVWPXVTIT-UHFFFAOYSA-M phosphinate Chemical compound [O-][PH2]=O ACVYVLVWPXVTIT-UHFFFAOYSA-M 0.000 description 4
• UEZVMMHDMIWARA-UHFFFAOYSA-M phosphonate Chemical compound [O-]P(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-M 0.000 description 4
• XYFCBTPGUUZFHI-UHFFFAOYSA-O phosphonium Chemical compound [PH4+] XYFCBTPGUUZFHI-UHFFFAOYSA-O 0.000 description 4
• 229920000642 polymer Polymers 0.000 description 4
• ROSDSFDQCJNGOL-UHFFFAOYSA-N protonated dimethyl amine Natural products CNC ROSDSFDQCJNGOL-UHFFFAOYSA-N 0.000 description 4
• 238000009938 salting Methods 0.000 description 4
• 239000001509 sodium citrate Substances 0.000 description 4
• NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 4
• 238000000527 sonication Methods 0.000 description 4
• 230000007704 transition Effects 0.000 description 4
• 210000002700 urine Anatomy 0.000 description 4
• 230000003442 weekly effect Effects 0.000 description 4
• ZVZRHYNDLMSKIV-UHFFFAOYSA-N 2-(trimethylazaniumyl)dodecanoate Chemical compound CCCCCCCCCCC(C([O-])=O)[N+](C)(C)C ZVZRHYNDLMSKIV-UHFFFAOYSA-N 0.000 description 3
• LMVGXBRDRZOPHA-UHFFFAOYSA-N 2-[dimethyl-[3-(16-methylheptadecanoylamino)propyl]azaniumyl]acetate Chemical compound CC(C)CCCCCCCCCCCCCCC(=O)NCCC[N+](C)(C)CC([O-])=O LMVGXBRDRZOPHA-UHFFFAOYSA-N 0.000 description 3
• 241000251468 Actinopterygii Species 0.000 description 3
• QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 3
• 108010078791 Carrier Proteins Proteins 0.000 description 3
• 108091006146 Channels Proteins 0.000 description 3
• 208000011231 Crohn disease Diseases 0.000 description 3
• 102000004190 Enzymes Human genes 0.000 description 3
• 108090000790 Enzymes Proteins 0.000 description 3
• 208000010228 Erectile Dysfunction Diseases 0.000 description 3
• PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
• VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
• 108090001030 Lipoproteins Proteins 0.000 description 3
• 102000004895 Lipoproteins Human genes 0.000 description 3
• OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
• 241000983409 Microbacterium terrae Species 0.000 description 3
• 241000187486 Mycobacterium flavescens Species 0.000 description 3
• 238000012408 PCR amplification Methods 0.000 description 3
• 241001494479 Pecora Species 0.000 description 3
• 229920001214 Polysorbate 60 Polymers 0.000 description 3
• HVUMOYIDDBPOLL-XWVZOOPGSA-N Sorbitan monostearate Chemical class CCCCCCCCCCCCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O HVUMOYIDDBPOLL-XWVZOOPGSA-N 0.000 description 3
• 230000002378 acidificating effect Effects 0.000 description 3
• 230000009056 active transport Effects 0.000 description 3
• 238000013019 agitation Methods 0.000 description 3
• 125000001931 aliphatic group Chemical group 0.000 description 3
• 125000002947 alkylene group Chemical group 0.000 description 3
• DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 3
• 235000020661 alpha-linolenic acid Nutrition 0.000 description 3
• 230000002547 anomalous effect Effects 0.000 description 3
• 239000012736 aqueous medium Substances 0.000 description 3
• 238000000376 autoradiography Methods 0.000 description 3
• 239000010480 babassu oil Substances 0.000 description 3
• 230000003542 behavioural effect Effects 0.000 description 3
• 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 description 3
• 230000002902 bimodal effect Effects 0.000 description 3
• 238000012742 biochemical analysis Methods 0.000 description 3
• 244000309464 bull Species 0.000 description 3
• 230000015556 catabolic process Effects 0.000 description 3
• 238000012512 characterization method Methods 0.000 description 3
• 238000005352 clarification Methods 0.000 description 3
• 238000010411 cooking Methods 0.000 description 3
• 238000005202 decontamination Methods 0.000 description 3
• 230000003588 decontaminative effect Effects 0.000 description 3
• 238000009792 diffusion process Methods 0.000 description 3
• 229940079593 drug Drugs 0.000 description 3
• 229940088598 enzyme Drugs 0.000 description 3
• 235000019688 fish Nutrition 0.000 description 3
• 238000007667 floating Methods 0.000 description 3
• 239000000499 gel Substances 0.000 description 3
• 230000002401 inhibitory effect Effects 0.000 description 3
• 238000011835 investigation Methods 0.000 description 3
• 238000004255 ion exchange chromatography Methods 0.000 description 3
• 229960004488 linolenic acid Drugs 0.000 description 3
• 230000000813 microbial effect Effects 0.000 description 3
• ONHFWHCMZAJCFB-UHFFFAOYSA-N myristamine oxide Chemical compound CCCCCCCCCCCCCC[N+](C)(C)[O-] ONHFWHCMZAJCFB-UHFFFAOYSA-N 0.000 description 3
• 125000000913 palmityl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 3
• 230000008506 pathogenesis Effects 0.000 description 3
• 230000000704 physical effect Effects 0.000 description 3
• 238000003672 processing method Methods 0.000 description 3
• 230000002035 prolonged effect Effects 0.000 description 3
• 230000001737 promoting effect Effects 0.000 description 3
• 238000001953 recrystallisation Methods 0.000 description 3
• 230000009467 reduction Effects 0.000 description 3
• 229920006395 saturated elastomer Polymers 0.000 description 3
• 210000002966 serum Anatomy 0.000 description 3
• 239000012064 sodium phosphate buffer Substances 0.000 description 3
• KBAFDSIZQYCDPK-UHFFFAOYSA-M sodium;octadecane-1-sulfonate Chemical compound [Na+].CCCCCCCCCCCCCCCCCCS([O-])(=O)=O KBAFDSIZQYCDPK-UHFFFAOYSA-M 0.000 description 3
• 239000007790 solid phase Substances 0.000 description 3
• 239000003549 soybean oil Substances 0.000 description 3
• 235000012424 soybean oil Nutrition 0.000 description 3
• 238000010186 staining Methods 0.000 description 3
• 238000010561 standard procedure Methods 0.000 description 3
• RWSOTUBLDIXVET-UHFFFAOYSA-O sulfonium Chemical compound [SH3+] RWSOTUBLDIXVET-UHFFFAOYSA-O 0.000 description 3
• 239000000725 suspension Substances 0.000 description 3
• 210000001519 tissue Anatomy 0.000 description 3
• 238000013518 transcription Methods 0.000 description 3
• 230000035897 transcription Effects 0.000 description 3
• 238000012546 transfer Methods 0.000 description 3
• 230000032258 transport Effects 0.000 description 3
• 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
• BLFZMXOCPASACY-UHFFFAOYSA-N 1,4-bis(propan-2-ylamino)anthracene-9,10-dione Chemical compound O=C1C2=CC=CC=C2C(=O)C2=C1C(NC(C)C)=CC=C2NC(C)C BLFZMXOCPASACY-UHFFFAOYSA-N 0.000 description 2
• DZLJLBZTQLMSJZ-UHFFFAOYSA-N 2-(trimethylazaniumyl)hexanoate Chemical compound CCCCC(C([O-])=O)[N+](C)(C)C DZLJLBZTQLMSJZ-UHFFFAOYSA-N 0.000 description 2
• HVYJSOSGTDINLW-UHFFFAOYSA-N 2-[dimethyl(octadecyl)azaniumyl]acetate Chemical compound CCCCCCCCCCCCCCCCCC[N+](C)(C)CC([O-])=O HVYJSOSGTDINLW-UHFFFAOYSA-N 0.000 description 2
• TWJNQYPJQDRXPH-UHFFFAOYSA-N 2-cyanobenzohydrazide Chemical compound NNC(=O)C1=CC=CC=C1C#N TWJNQYPJQDRXPH-UHFFFAOYSA-N 0.000 description 2
• HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 description 2
• SATHPVQTSSUFFW-UHFFFAOYSA-N 4-[6-[(3,5-dihydroxy-4-methoxyoxan-2-yl)oxymethyl]-3,5-dihydroxy-4-methoxyoxan-2-yl]oxy-2-(hydroxymethyl)-6-methyloxane-3,5-diol Chemical compound OC1C(OC)C(O)COC1OCC1C(O)C(OC)C(O)C(OC2C(C(CO)OC(C)C2O)O)O1 SATHPVQTSSUFFW-UHFFFAOYSA-N 0.000 description 2
• QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
• 229920000189 Arabinogalactan Polymers 0.000 description 2
• 239000001904 Arabinogalactan Substances 0.000 description 2
• DPUOLQHDNGRHBS-UHFFFAOYSA-N Brassidinsaeure Natural products CCCCCCCCC=CCCCCCCCCCCCC(O)=O DPUOLQHDNGRHBS-UHFFFAOYSA-N 0.000 description 2
• 229930186147 Cephalosporin Natural products 0.000 description 2
• 244000026610 Cynodon dactylon var. affinis Species 0.000 description 2
• GHVNFZFCNZKVNT-UHFFFAOYSA-N Decanoic acid Natural products CCCCCCCCCC(O)=O GHVNFZFCNZKVNT-UHFFFAOYSA-N 0.000 description 2
• 102000016911 Deoxyribonucleases Human genes 0.000 description 2
• 108010053770 Deoxyribonucleases Proteins 0.000 description 2
• AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 description 2
• NIQCNGHVCWTJSM-UHFFFAOYSA-N Dimethyl phthalate Chemical compound COC(=O)C1=CC=CC=C1C(=O)OC NIQCNGHVCWTJSM-UHFFFAOYSA-N 0.000 description 2
• 241000196324 Embryophyta Species 0.000 description 2
• URXZXNYJPAJJOQ-UHFFFAOYSA-N Erucic acid Natural products CCCCCCC=CCCCCCCCCCCCC(O)=O URXZXNYJPAJJOQ-UHFFFAOYSA-N 0.000 description 2
• 229920001917 Ficoll Polymers 0.000 description 2
• 108010031186 Glycoside Hydrolases Proteins 0.000 description 2
• 102000005744 Glycoside Hydrolases Human genes 0.000 description 2
• 206010018691 Granuloma Diseases 0.000 description 2
• 241000282412 Homo Species 0.000 description 2
• LTYOQGRJFJAKNA-KKIMTKSISA-N Malonyl CoA Natural products S(C(=O)CC(=O)O)CCNC(=O)CCNC(=O)[C@@H](O)C(CO[P@](=O)(O[P@](=O)(OC[C@H]1[C@@H](OP(=O)(O)O)[C@@H](O)[C@@H](n2c3ncnc(N)c3nc2)O1)O)O)(C)C LTYOQGRJFJAKNA-KKIMTKSISA-N 0.000 description 2
• 102000016943 Muramidase Human genes 0.000 description 2
• 108010014251 Muramidase Proteins 0.000 description 2
• 235000021360 Myristic acid Nutrition 0.000 description 2
• TUNFSRHWOTWDNC-UHFFFAOYSA-N Myristic acid Natural products CCCCCCCCCCCCCC(O)=O TUNFSRHWOTWDNC-UHFFFAOYSA-N 0.000 description 2
• 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 2
• ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
• 235000019484 Rapeseed oil Nutrition 0.000 description 2
• 235000004443 Ricinus communis Nutrition 0.000 description 2
• 241000282849 Ruminantia Species 0.000 description 2
• 229930006000 Sucrose Natural products 0.000 description 2
• CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
• LWZFANDGMFTDAV-BURFUSLBSA-N [(2r)-2-[(2r,3r,4s)-3,4-dihydroxyoxolan-2-yl]-2-hydroxyethyl] dodecanoate Chemical compound CCCCCCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O LWZFANDGMFTDAV-BURFUSLBSA-N 0.000 description 2
• 230000001594 aberrant effect Effects 0.000 description 2
• 206010000269 abscess Diseases 0.000 description 2
• ORILYTVJVMAKLC-UHFFFAOYSA-N adamantane Chemical compound C1C(C2)CC3CC1CC2C3 ORILYTVJVMAKLC-UHFFFAOYSA-N 0.000 description 2
• 239000000654 additive Substances 0.000 description 2
• 238000000246 agarose gel electrophoresis Methods 0.000 description 2
• 125000005211 alkyl trimethyl ammonium group Chemical group 0.000 description 2
• 238000012197 amplification kit Methods 0.000 description 2
• 125000000129 anionic group Chemical group 0.000 description 2
• 239000007864 aqueous solution Substances 0.000 description 2
• 235000019312 arabinogalactan Nutrition 0.000 description 2
• 229910052786 argon Inorganic materials 0.000 description 2
• 210000003567 ascitic fluid Anatomy 0.000 description 2
• 239000011324 bead Substances 0.000 description 2
• 235000015278 beef Nutrition 0.000 description 2
• 229960000686 benzalkonium chloride Drugs 0.000 description 2
• CADWTSSKOVRVJC-UHFFFAOYSA-N benzyl(dimethyl)azanium;chloride Chemical compound [Cl-].C[NH+](C)CC1=CC=CC=C1 CADWTSSKOVRVJC-UHFFFAOYSA-N 0.000 description 2
• 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 2
• 229940124587 cephalosporin Drugs 0.000 description 2
• 230000003196 chaotropic effect Effects 0.000 description 2
• 235000013351 cheese Nutrition 0.000 description 2
• 239000013522 chelant Substances 0.000 description 2
• 229920001429 chelating resin Polymers 0.000 description 2
• 239000007795 chemical reaction product Substances 0.000 description 2
• 239000002285 corn oil Substances 0.000 description 2
• 235000005687 corn oil Nutrition 0.000 description 2
• 239000011557 critical solution Substances 0.000 description 2
• 238000006731 degradation reaction Methods 0.000 description 2
• 230000018044 dehydration Effects 0.000 description 2
• 238000006297 dehydration reaction Methods 0.000 description 2
• LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
• 238000006073 displacement reaction Methods 0.000 description 2
• 239000012153 distilled water Substances 0.000 description 2
• 125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 2
• 238000002651 drug therapy Methods 0.000 description 2
• 229910001651 emery Inorganic materials 0.000 description 2
• 239000003995 emulsifying agent Substances 0.000 description 2
• DPUOLQHDNGRHBS-KTKRTIGZSA-N erucic acid Chemical compound CCCCCCCC\C=C/CCCCCCCCCCCC(O)=O DPUOLQHDNGRHBS-KTKRTIGZSA-N 0.000 description 2
• 150000002148 esters Chemical class 0.000 description 2
• ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 2
• 229960005542 ethidium bromide Drugs 0.000 description 2
• XIMFCGSNSKXPBO-UHFFFAOYSA-N ethyl 2-bromobutanoate Chemical compound CCOC(=O)C(Br)CC XIMFCGSNSKXPBO-UHFFFAOYSA-N 0.000 description 2
• 230000007717 exclusion Effects 0.000 description 2
• 238000013401 experimental design Methods 0.000 description 2
• 210000000416 exudates and transudate Anatomy 0.000 description 2
• 238000009472 formulation Methods 0.000 description 2
• 238000005194 fractionation Methods 0.000 description 2
• 230000002496 gastric effect Effects 0.000 description 2
• 238000005227 gel permeation chromatography Methods 0.000 description 2
• 239000011521 glass Substances 0.000 description 2
• 125000004051 hexyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 2
• 244000052637 human pathogen Species 0.000 description 2
• 229930195733 hydrocarbon Natural products 0.000 description 2
• 150000002430 hydrocarbons Chemical group 0.000 description 2
• 230000033444 hydroxylation Effects 0.000 description 2
• 238000005805 hydroxylation reaction Methods 0.000 description 2
• MTNDZQHUAFNZQY-UHFFFAOYSA-N imidazoline Chemical compound C1CN=CN1 MTNDZQHUAFNZQY-UHFFFAOYSA-N 0.000 description 2
• 238000003018 immunoassay Methods 0.000 description 2
• 230000003993 interaction Effects 0.000 description 2
• 230000000968 intestinal effect Effects 0.000 description 2
• 210000000936 intestine Anatomy 0.000 description 2
• 125000003010 ionic group Chemical group 0.000 description 2
• 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 2
• 230000003902 lesion Effects 0.000 description 2
• 230000006372 lipid accumulation Effects 0.000 description 2
• 239000004325 lysozyme Substances 0.000 description 2
• 229960000274 lysozyme Drugs 0.000 description 2
• 235000010335 lysozyme Nutrition 0.000 description 2
• 238000003754 machining Methods 0.000 description 2
• 229910001629 magnesium chloride Inorganic materials 0.000 description 2
• LTYOQGRJFJAKNA-DVVLENMVSA-N malonyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(O)=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 LTYOQGRJFJAKNA-DVVLENMVSA-N 0.000 description 2
• 230000001404 mediated effect Effects 0.000 description 2
• 230000003278 mimic effect Effects 0.000 description 2
• 238000010369 molecular cloning Methods 0.000 description 2
• 239000000178 monomer Substances 0.000 description 2
• 125000001421 myristyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 2
• APVPOHHVBBYQAV-UHFFFAOYSA-N n-(4-aminophenyl)sulfonyloctadecanamide Chemical compound CCCCCCCCCCCCCCCCCC(=O)NS(=O)(=O)C1=CC=C(N)C=C1 APVPOHHVBBYQAV-UHFFFAOYSA-N 0.000 description 2
• DVEKCXOJTLDBFE-UHFFFAOYSA-N n-dodecyl-n,n-dimethylglycinate Chemical compound CCCCCCCCCCCC[N+](C)(C)CC([O-])=O DVEKCXOJTLDBFE-UHFFFAOYSA-N 0.000 description 2
• VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 2
• 230000035699 permeability Effects 0.000 description 2
• 230000002085 persistent effect Effects 0.000 description 2
• 239000002953 phosphate buffered saline Substances 0.000 description 2
• 244000144977 poultry Species 0.000 description 2
• 230000001376 precipitating effect Effects 0.000 description 2
• 229940055019 propionibacterium acne Drugs 0.000 description 2
• 238000011160 research Methods 0.000 description 2
• 238000012216 screening Methods 0.000 description 2
• 239000000377 silicon dioxide Substances 0.000 description 2
• DAJSVUQLFFJUSX-UHFFFAOYSA-M sodium;dodecane-1-sulfonate Chemical compound [Na+].CCCCCCCCCCCCS([O-])(=O)=O DAJSVUQLFFJUSX-UHFFFAOYSA-M 0.000 description 2
• AYFACLKQYVTXNS-UHFFFAOYSA-M sodium;tetradecane-1-sulfonate Chemical compound [Na+].CCCCCCCCCCCCCCS([O-])(=O)=O AYFACLKQYVTXNS-UHFFFAOYSA-M 0.000 description 2
• 238000000638 solvent extraction Methods 0.000 description 2
• 238000001228 spectrum Methods 0.000 description 2
• 238000003860 storage Methods 0.000 description 2
• 239000005720 sucrose Substances 0.000 description 2
• GETQZCLCWQTVFV-UHFFFAOYSA-N trimethylamine Chemical group CN(C)C GETQZCLCWQTVFV-UHFFFAOYSA-N 0.000 description 2
• 239000001226 triphosphate Substances 0.000 description 2
• 235000011178 triphosphate Nutrition 0.000 description 2
• UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 2
• JNYAEWCLZODPBN-JGWLITMVSA-N (2r,3r,4s)-2-[(1r)-1,2-dihydroxyethyl]oxolane-3,4-diol Chemical compound OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O JNYAEWCLZODPBN-JGWLITMVSA-N 0.000 description 1
• CHEANNSDVJOIBS-MHZLTWQESA-N (3s)-3-cyclopropyl-3-[3-[[3-(5,5-dimethylcyclopenten-1-yl)-4-(2-fluoro-5-methoxyphenyl)phenyl]methoxy]phenyl]propanoic acid Chemical compound COC1=CC=C(F)C(C=2C(=CC(COC=3C=C(C=CC=3)[C@@H](CC(O)=O)C3CC3)=CC=2)C=2C(CCC=2)(C)C)=C1 CHEANNSDVJOIBS-MHZLTWQESA-N 0.000 description 1
• OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 1
• QCTZUSWOKFCWNB-QXMHVHEDSA-N (z)-n,n-dimethyloctadec-9-en-1-amine oxide Chemical compound CCCCCCCC\C=C/CCCCCCCC[N+](C)(C)[O-] QCTZUSWOKFCWNB-QXMHVHEDSA-N 0.000 description 1
• NOGLQXZIGOQIBD-UHFFFAOYSA-N 1-(dimethylazaniumyl)propane-1-sulfonate Chemical group CCC(N(C)C)S(O)(=O)=O NOGLQXZIGOQIBD-UHFFFAOYSA-N 0.000 description 1
• GYSCBCSGKXNZRH-UHFFFAOYSA-N 1-benzothiophene-2-carboxamide Chemical compound C1=CC=C2SC(C(=O)N)=CC2=C1 GYSCBCSGKXNZRH-UHFFFAOYSA-N 0.000 description 1
• LTSWUFKUZPPYEG-UHFFFAOYSA-N 1-decoxydecane Chemical compound CCCCCCCCCCOCCCCCCCCCC LTSWUFKUZPPYEG-UHFFFAOYSA-N 0.000 description 1
• NAQCLDVZUBWFFK-UHFFFAOYSA-N 2-(trimethylazaniumyl)hexadecanoate Chemical compound CCCCCCCCCCCCCCC(C([O-])=O)[N+](C)(C)C NAQCLDVZUBWFFK-UHFFFAOYSA-N 0.000 description 1
• LVBSECZFJBGOCR-UHFFFAOYSA-N 2-(trimethylazaniumyl)tetradecanoate Chemical compound CCCCCCCCCCCCC(C([O-])=O)[N+](C)(C)C LVBSECZFJBGOCR-UHFFFAOYSA-N 0.000 description 1
• MGYUQZIGNZFZJS-KTKRTIGZSA-N 2-[2-[(z)-octadec-9-enoxy]ethoxy]ethanol Chemical compound CCCCCCCC\C=C/CCCCCCCCOCCOCCO MGYUQZIGNZFZJS-KTKRTIGZSA-N 0.000 description 1
• JKXYOQDLERSFPT-UHFFFAOYSA-N 2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(2-octadecoxyethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethanol Chemical compound CCCCCCCCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO JKXYOQDLERSFPT-UHFFFAOYSA-N 0.000 description 1
• IEQAICDLOKRSRL-UHFFFAOYSA-N 2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(2-dodecoxyethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethanol Chemical compound CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO IEQAICDLOKRSRL-UHFFFAOYSA-N 0.000 description 1
• OTKWLUKIHNEGIG-UHFFFAOYSA-N 2-[3-(hexadecanoylamino)propyl-dimethylazaniumyl]acetate Chemical compound CCCCCCCCCCCCCCCC(=O)NCCC[N+](C)(C)CC([O-])=O OTKWLUKIHNEGIG-UHFFFAOYSA-N 0.000 description 1
• NPKLJZUIYWRNMV-UHFFFAOYSA-N 2-[decyl(dimethyl)azaniumyl]acetate Chemical compound CCCCCCCCCC[N+](C)(C)CC([O-])=O NPKLJZUIYWRNMV-UHFFFAOYSA-N 0.000 description 1
• KKMIHKCGXQMFEU-UHFFFAOYSA-N 2-[dimethyl(tetradecyl)azaniumyl]acetate Chemical compound CCCCCCCCCCCCCC[N+](C)(C)CC([O-])=O KKMIHKCGXQMFEU-UHFFFAOYSA-N 0.000 description 1
• VSAFLJOWIKSTNB-UHFFFAOYSA-N 2-[dimethyl(undecyl)azaniumyl]acetate Chemical compound CCCCCCCCCCC[N+](C)(C)CC(=O)[O-] VSAFLJOWIKSTNB-UHFFFAOYSA-N 0.000 description 1
• BHZBVWCLMYQFQX-UHFFFAOYSA-N 2-octadecyloxirane Chemical compound CCCCCCCCCCCCCCCCCCC1CO1 BHZBVWCLMYQFQX-UHFFFAOYSA-N 0.000 description 1
• NKOTXYPTXKUCDL-UHFFFAOYSA-N 4-(trifluoromethyl)pyrimidin-2-amine Chemical compound NC1=NC=CC(C(F)(F)F)=N1 NKOTXYPTXKUCDL-UHFFFAOYSA-N 0.000 description 1
• OYHQOLUKZRVURQ-UHFFFAOYSA-N 9,12-Octadecadienoic Acid Chemical compound CCCCCC=CCC=CCCCCCCCC(O)=O OYHQOLUKZRVURQ-UHFFFAOYSA-N 0.000 description 1
• 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
• 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
• 244000080565 Attalea funifera Species 0.000 description 1
• 235000005587 Attalea funifera Nutrition 0.000 description 1
• ROFVEXUMMXZLPA-UHFFFAOYSA-N Bipyridyl Chemical compound N1=CC=CC=C1C1=CC=CC=N1 ROFVEXUMMXZLPA-UHFFFAOYSA-N 0.000 description 1
• 244000060924 Brassica campestris Species 0.000 description 1
• 235000005637 Brassica campestris Nutrition 0.000 description 1
• CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
• 241000282472 Canis lupus familiaris Species 0.000 description 1
• 241000283707 Capra Species 0.000 description 1
• 239000004215 Carbon black (E152) Substances 0.000 description 1
• 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
• 102000016938 Catalase Human genes 0.000 description 1
• 108010053835 Catalase Proteins 0.000 description 1
• 241000282693 Cercopithecidae Species 0.000 description 1
• 241000282994 Cervidae Species 0.000 description 1
• CXRFDZFCGOPDTD-UHFFFAOYSA-M Cetrimide Chemical compound [Br-].CCCCCCCCCCCCCC[N+](C)(C)C CXRFDZFCGOPDTD-UHFFFAOYSA-M 0.000 description 1
• 244000060011 Cocos nucifera Species 0.000 description 1
• 235000013162 Cocos nucifera Nutrition 0.000 description 1
• 208000035473 Communicable disease Diseases 0.000 description 1
• 241000186216 Corynebacterium Species 0.000 description 1
• 241000186245 Corynebacterium xerosis Species 0.000 description 1
• SNRUBQQJIBEYMU-UHFFFAOYSA-N Dodecane Natural products CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 1
• 206010059866 Drug resistance Diseases 0.000 description 1
• 241000283086 Equidae Species 0.000 description 1
• 241000588724 Escherichia coli Species 0.000 description 1
• IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
• 241000282326 Felis catus Species 0.000 description 1
• 241000192016 Finegoldia magna Species 0.000 description 1
• 241000233866 Fungi Species 0.000 description 1
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
• 229920002683 Glycosaminoglycan Polymers 0.000 description 1
• 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
• UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
• DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
• 206010061218 Inflammation Diseases 0.000 description 1
• 101150046735 LEPR gene Proteins 0.000 description 1
• 101150063827 LEPROT gene Proteins 0.000 description 1
• 208000032420 Latent Infection Diseases 0.000 description 1
• 239000005639 Lauric acid Substances 0.000 description 1
• 241000270322 Lepidosauria Species 0.000 description 1
• 208000019693 Lung disease Diseases 0.000 description 1
• 241001467578 Microbacterium Species 0.000 description 1
• 244000131360 Morinda citrifolia Species 0.000 description 1
• 108010085220 Multiprotein Complexes Proteins 0.000 description 1
• 102000007474 Multiprotein Complexes Human genes 0.000 description 1
• MSFSPUZXLOGKHJ-UHFFFAOYSA-N Muraminsaeure Natural products OC(=O)C(C)OC1C(N)C(O)OC(CO)C1O MSFSPUZXLOGKHJ-UHFFFAOYSA-N 0.000 description 1
• 206010071401 Mycobacterium chelonae infection Diseases 0.000 description 1
• 241001302239 Mycobacterium tuberculosis complex Species 0.000 description 1
• QGCUAFIULMNFPJ-UHFFFAOYSA-N Myristamidopropyl betaine Chemical compound CCCCCCCCCCCCCC(=O)NCCC[N+](C)(C)CC([O-])=O QGCUAFIULMNFPJ-UHFFFAOYSA-N 0.000 description 1
• FSVCELGFZIQNCK-UHFFFAOYSA-N N,N-bis(2-hydroxyethyl)glycine Chemical compound OCCN(CCO)CC(O)=O FSVCELGFZIQNCK-UHFFFAOYSA-N 0.000 description 1
• 229920002274 Nalgene Polymers 0.000 description 1
• 241000772415 Neovison vison Species 0.000 description 1
• 241000187654 Nocardia Species 0.000 description 1
• 241000187678 Nocardia asteroides Species 0.000 description 1
• 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
• 108091028043 Nucleic acid sequence Proteins 0.000 description 1
• 239000004677 Nylon Substances 0.000 description 1
• 238000002944 PCR assay Methods 0.000 description 1
• 208000026681 Paratuberculosis Diseases 0.000 description 1
• 108010013639 Peptidoglycan Proteins 0.000 description 1
• XYFCBTPGUUZFHI-UHFFFAOYSA-N Phosphine Natural products P XYFCBTPGUUZFHI-UHFFFAOYSA-N 0.000 description 1
• 241000255969 Pieris brassicae Species 0.000 description 1
• 108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 description 1
• 102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 1
• 241001135223 Prevotella melaninogenica Species 0.000 description 1
• 241000283080 Proboscidea <mammal> Species 0.000 description 1
• 241000186334 Propionibacterium freudenreichii subsp. shermanii Species 0.000 description 1
• 241000158504 Rhodococcus hoagii Species 0.000 description 1
• 108010039491 Ricin Proteins 0.000 description 1
• 240000000528 Ricinus communis Species 0.000 description 1
• 241000283984 Rodentia Species 0.000 description 1
• VBIIFPGSPJYLRR-UHFFFAOYSA-M Stearyltrimethylammonium chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCCCC[N+](C)(C)C VBIIFPGSPJYLRR-UHFFFAOYSA-M 0.000 description 1
• 241000194019 Streptococcus mutans Species 0.000 description 1
• 206010042566 Superinfection Diseases 0.000 description 1
• 241000282898 Sus scrofa Species 0.000 description 1
• 108010006785 Taq Polymerase Proteins 0.000 description 1
• 244000299461 Theobroma cacao Species 0.000 description 1
• 235000009470 Theobroma cacao Nutrition 0.000 description 1
• 244000098338 Triticum aestivum Species 0.000 description 1
• 102000006943 Uracil-DNA Glycosidase Human genes 0.000 description 1
• 108010072685 Uracil-DNA Glycosidase Proteins 0.000 description 1
• 235000012545 Vaccinium macrocarpon Nutrition 0.000 description 1
• 244000291414 Vaccinium oxycoccus Species 0.000 description 1
• 235000002118 Vaccinium oxycoccus Nutrition 0.000 description 1
• 235000007244 Zea mays Nutrition 0.000 description 1
• QWXOJIDBSHLIFI-UHFFFAOYSA-N [3-(1-chloro-3'-methoxyspiro[adamantane-4,4'-dioxetane]-3'-yl)phenyl] dihydrogen phosphate Chemical compound O1OC2(C3CC4CC2CC(Cl)(C4)C3)C1(OC)C1=CC=CC(OP(O)(O)=O)=C1 QWXOJIDBSHLIFI-UHFFFAOYSA-N 0.000 description 1
• 230000005856 abnormality Effects 0.000 description 1
• 239000004480 active ingredient Substances 0.000 description 1
• 239000011149 active material Substances 0.000 description 1
• 230000000996 additive effect Effects 0.000 description 1
• 230000016571 aggressive behavior Effects 0.000 description 1
• 150000008052 alkyl sulfonates Chemical class 0.000 description 1
• 150000001408 amides Chemical group 0.000 description 1
• 239000002280 amphoteric surfactant Substances 0.000 description 1
• 210000003484 anatomy Anatomy 0.000 description 1
• 150000001449 anionic compounds Chemical class 0.000 description 1
• 239000003945 anionic surfactant Substances 0.000 description 1
• 238000000137 annealing Methods 0.000 description 1
• 150000001454 anthracenes Chemical class 0.000 description 1
• 239000004599 antimicrobial Substances 0.000 description 1
• 238000013459 approach Methods 0.000 description 1
• 239000012062 aqueous buffer Substances 0.000 description 1
• JPIYZTWMUGTEHX-UHFFFAOYSA-N auramine O free base Chemical compound C1=CC(N(C)C)=CC=C1C(=N)C1=CC=C(N(C)C)C=C1 JPIYZTWMUGTEHX-UHFFFAOYSA-N 0.000 description 1
• UBMXFGVZFUYLSN-UHFFFAOYSA-N azanium;propane-1-sulfonate Chemical group [NH4+].CCCS([O-])(=O)=O UBMXFGVZFUYLSN-UHFFFAOYSA-N 0.000 description 1
• 125000000656 azaniumyl group Chemical group [H][N+]([H])([H])[*] 0.000 description 1
• 244000052616 bacterial pathogen Species 0.000 description 1
• 230000004888 barrier function Effects 0.000 description 1
• KHSLHYAUZSPBIU-UHFFFAOYSA-M benzododecinium bromide Chemical compound [Br-].CCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 KHSLHYAUZSPBIU-UHFFFAOYSA-M 0.000 description 1
• 229940000635 beta-alanine Drugs 0.000 description 1
• 239000003833 bile salt Substances 0.000 description 1
• 238000010256 biochemical assay Methods 0.000 description 1
• 239000012620 biological material Substances 0.000 description 1
• 238000001574 biopsy Methods 0.000 description 1
• 208000011090 bird disease Diseases 0.000 description 1
• 210000001185 bone marrow Anatomy 0.000 description 1
• 125000004106 butoxy group Chemical group [*]OC([H])([H])C([H])([H])C(C([H])([H])[H])([H])[H] 0.000 description 1
• 239000006227 byproduct Substances 0.000 description 1
• 239000002775 capsule Substances 0.000 description 1
• 229910052799 carbon Inorganic materials 0.000 description 1
• 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
• 150000007942 carboxylates Chemical class 0.000 description 1
• FLBSTBFNHMWAQO-UHFFFAOYSA-N carboxymethyl-dimethyl-octadecanoylazanium;hydroxide Chemical compound [OH-].CCCCCCCCCCCCCCCCCC(=O)[N+](C)(C)CC(O)=O FLBSTBFNHMWAQO-UHFFFAOYSA-N 0.000 description 1
• 239000003093 cationic surfactant Substances 0.000 description 1
• 238000004113 cell culture Methods 0.000 description 1
• 230000006037 cell lysis Effects 0.000 description 1
• 210000000170 cell membrane Anatomy 0.000 description 1
• 229920006184 cellulose methylcellulose Polymers 0.000 description 1
• 150000001780 cephalosporins Chemical class 0.000 description 1
• 239000011153 ceramic matrix composite Substances 0.000 description 1
• 235000013339 cereals Nutrition 0.000 description 1
• 238000009390 chemical decontamination Methods 0.000 description 1
• 238000012710 chemistry, manufacturing and control Methods 0.000 description 1
• 238000003759 clinical diagnosis Methods 0.000 description 1
• 239000011248 coating agent Substances 0.000 description 1
• 238000000576 coating method Methods 0.000 description 1
• 238000004440 column chromatography Methods 0.000 description 1
• 230000000295 complement effect Effects 0.000 description 1
• 239000012141 concentrate Substances 0.000 description 1
• 230000001143 conditioned effect Effects 0.000 description 1
• 238000012790 confirmation Methods 0.000 description 1
• 108091036078 conserved sequence Proteins 0.000 description 1
• 238000010276 construction Methods 0.000 description 1
• 238000007796 conventional method Methods 0.000 description 1
• 230000002596 correlated effect Effects 0.000 description 1
• 235000004634 cranberry Nutrition 0.000 description 1
• 238000002425 crystallisation Methods 0.000 description 1
• 230000008025 crystallization Effects 0.000 description 1
• 238000012136 culture method Methods 0.000 description 1
• 230000001351 cycling effect Effects 0.000 description 1
• 125000001559 cyclopropyl group Chemical group [H]C1([H])C([H])([H])C1([H])* 0.000 description 1
• SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
• SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
• RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
• HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
• NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
• 235000013365 dairy product Nutrition 0.000 description 1
• 238000010908 decantation Methods 0.000 description 1
• 230000003247 decreasing effect Effects 0.000 description 1
• KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 description 1
• 229960003964 deoxycholic acid Drugs 0.000 description 1
• KXGVEGMKQFWNSR-UHFFFAOYSA-N deoxycholic acid Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)C(O)C2 KXGVEGMKQFWNSR-UHFFFAOYSA-N 0.000 description 1
• 238000011161 development Methods 0.000 description 1
• 239000008121 dextrose Substances 0.000 description 1
• 238000010586 diagram Methods 0.000 description 1
• 125000004177 diethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
• 125000001664 diethylamino group Chemical group [H]C([H])([H])C([H])([H])N(*)C([H])([H])C([H])([H])[H] 0.000 description 1
• 230000004069 differentiation Effects 0.000 description 1
• 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 1
• FBSAITBEAPNWJG-UHFFFAOYSA-N dimethyl phthalate Natural products CC(=O)OC1=CC=CC=C1OC(C)=O FBSAITBEAPNWJG-UHFFFAOYSA-N 0.000 description 1
• RWWMTLBGGWWMJA-UHFFFAOYSA-N dimethyl-octadecyl-(3-sulfopropyl)azanium;hydroxide Chemical compound [OH-].CCCCCCCCCCCCCCCCCC[N+](C)(C)CCCS(O)(=O)=O RWWMTLBGGWWMJA-UHFFFAOYSA-N 0.000 description 1
• 229960001826 dimethylphthalate Drugs 0.000 description 1
• 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
• 229910000397 disodium phosphate Inorganic materials 0.000 description 1
• 230000009977 dual effect Effects 0.000 description 1
• 235000013601 eggs Nutrition 0.000 description 1
• 230000008030 elimination Effects 0.000 description 1
• 238000003379 elimination reaction Methods 0.000 description 1
• 238000004945 emulsification Methods 0.000 description 1
• 230000002708 enhancing effect Effects 0.000 description 1
• 230000007613 environmental effect Effects 0.000 description 1
• 230000006862 enzymatic digestion Effects 0.000 description 1
• 125000001033 ether group Chemical group 0.000 description 1
• 150000002170 ethers Chemical group 0.000 description 1
• 238000011156 evaluation Methods 0.000 description 1
• 238000002270 exclusion chromatography Methods 0.000 description 1
• 210000003414 extremity Anatomy 0.000 description 1
• 210000002950 fibroblast Anatomy 0.000 description 1
• 238000005189 flocculation Methods 0.000 description 1
• 230000016615 flocculation Effects 0.000 description 1
• 238000005188 flotation Methods 0.000 description 1
• GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
• 239000012634 fragment Substances 0.000 description 1
• 125000000524 functional group Chemical group 0.000 description 1
• 238000001030 gas--liquid chromatography Methods 0.000 description 1
• 230000002068 genetic effect Effects 0.000 description 1
• 210000004907 gland Anatomy 0.000 description 1
• 229960001031 glucose Drugs 0.000 description 1
• 150000004676 glycans Chemical class 0.000 description 1
• ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
• 238000010438 heat treatment Methods 0.000 description 1
• 229940094991 herring sperm dna Drugs 0.000 description 1
• 210000005260 human cell Anatomy 0.000 description 1
• 239000001257 hydrogen Substances 0.000 description 1
• 229910052739 hydrogen Inorganic materials 0.000 description 1
• 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
• XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
• 125000004029 hydroxymethyl group Chemical group [H]OC([H])([H])* 0.000 description 1
• 150000002462 imidazolines Chemical class 0.000 description 1
• 230000002458 infectious effect Effects 0.000 description 1
• 208000027866 inflammatory disease Diseases 0.000 description 1
• 230000004054 inflammatory process Effects 0.000 description 1
• 238000011850 initial investigation Methods 0.000 description 1
• 238000011081 inoculation Methods 0.000 description 1
• 238000003780 insertion Methods 0.000 description 1
• 230000037431 insertion Effects 0.000 description 1
• 239000013067 intermediate product Substances 0.000 description 1
• 238000003771 laboratory diagnosis Methods 0.000 description 1
• 239000004973 liquid crystal related substance Substances 0.000 description 1
• 238000009630 liquid culture Methods 0.000 description 1
• 244000144972 livestock Species 0.000 description 1
• 230000007774 longterm Effects 0.000 description 1
• 210000002751 lymph Anatomy 0.000 description 1
• 108010026228 mRNA guanylyltransferase Proteins 0.000 description 1
• 239000003550 marker Substances 0.000 description 1
• 230000004060 metabolic process Effects 0.000 description 1
• UFQQDNMQADCHGH-UHFFFAOYSA-N methyl 2-bromobutanoate Chemical compound CCC(Br)C(=O)OC UFQQDNMQADCHGH-UHFFFAOYSA-N 0.000 description 1
• SVSKWGDTQMPPQE-UHFFFAOYSA-N methyl 2-iodobutanoate Chemical compound CCC(I)C(=O)OC SVSKWGDTQMPPQE-UHFFFAOYSA-N 0.000 description 1
• 238000001000 micrograph Methods 0.000 description 1
• 235000013336 milk Nutrition 0.000 description 1
• 239000008267 milk Substances 0.000 description 1
• 210000004080 milk Anatomy 0.000 description 1
• 210000001616 monocyte Anatomy 0.000 description 1
• 230000000877 morphologic effect Effects 0.000 description 1
• 210000003097 mucus Anatomy 0.000 description 1
• VMESOKCXSYNAKD-UHFFFAOYSA-N n,n-dimethylhydroxylamine Chemical class CN(C)O VMESOKCXSYNAKD-UHFFFAOYSA-N 0.000 description 1
• VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical group CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 1
• 125000004123 n-propyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
• 238000007857 nested PCR Methods 0.000 description 1
• 238000006386 neutralization reaction Methods 0.000 description 1
• 230000003472 neutralizing effect Effects 0.000 description 1
• 238000009828 non-uniform distribution Methods 0.000 description 1
• 235000017524 noni Nutrition 0.000 description 1
• 239000002736 nonionic surfactant Substances 0.000 description 1
• HLERILKGMXJNBU-UHFFFAOYSA-N norvaline betaine Chemical compound CCCC(C([O-])=O)[N+](C)(C)C HLERILKGMXJNBU-UHFFFAOYSA-N 0.000 description 1
• 239000002853 nucleic acid probe Substances 0.000 description 1
• 229920001778 nylon Polymers 0.000 description 1
• 229940049964 oleate Drugs 0.000 description 1
• 244000039328 opportunistic pathogen Species 0.000 description 1
• 150000002894 organic compounds Chemical class 0.000 description 1
• 125000000962 organic group Chemical group 0.000 description 1
• 235000016046 other dairy product Nutrition 0.000 description 1
• MPQXHAGKBWFSNV-UHFFFAOYSA-N oxidophosphanium Chemical class [PH3]=O MPQXHAGKBWFSNV-UHFFFAOYSA-N 0.000 description 1
• 230000000803 paradoxical effect Effects 0.000 description 1
• 230000037361 pathway Effects 0.000 description 1
• 239000013610 patient sample Substances 0.000 description 1
• 125000001147 pentyl group Chemical group C(CCCC)* 0.000 description 1
• RXNXLAHQOVLMIE-UHFFFAOYSA-N phenyl 10-methylacridin-10-ium-9-carboxylate Chemical compound C12=CC=CC=C2[N+](C)=C2C=CC=CC2=C1C(=O)OC1=CC=CC=C1 RXNXLAHQOVLMIE-UHFFFAOYSA-N 0.000 description 1
• 239000008363 phosphate buffer Substances 0.000 description 1
• 229910000073 phosphorus hydride Inorganic materials 0.000 description 1
• 230000010399 physical interaction Effects 0.000 description 1
• 230000004260 plant-type cell wall biogenesis Effects 0.000 description 1
• 229920003023 plastic Polymers 0.000 description 1
• 239000004033 plastic Substances 0.000 description 1
• 238000007747 plating Methods 0.000 description 1
• 229920001983 poloxamer Polymers 0.000 description 1
• 229940100474 polyethylene glycol 1450 Drugs 0.000 description 1
• 229920001282 polysaccharide Polymers 0.000 description 1
• 239000005017 polysaccharide Substances 0.000 description 1
• 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
• 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
• 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
• 238000011176 pooling Methods 0.000 description 1
• 239000011148 porous material Substances 0.000 description 1
• FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical class [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 1
• 239000008057 potassium phosphate buffer Substances 0.000 description 1
• 230000003389 potentiating effect Effects 0.000 description 1
• 230000002265 prevention Effects 0.000 description 1
• 230000035755 proliferation Effects 0.000 description 1
• 239000008213 purified water Substances 0.000 description 1
• 238000000275 quality assurance Methods 0.000 description 1
• 125000001453 quaternary ammonium group Chemical class 0.000 description 1
• 150000003242 quaternary ammonium salts Chemical class 0.000 description 1
• 150000003254 radicals Chemical class 0.000 description 1
• 230000002285 radioactive effect Effects 0.000 description 1
• 108091035233 repetitive DNA sequence Proteins 0.000 description 1
• 102000053632 repetitive DNA sequence Human genes 0.000 description 1
• 230000010076 replication Effects 0.000 description 1
• 230000006903 response to temperature Effects 0.000 description 1
• PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
• YAYGSLOSTXKUBW-UHFFFAOYSA-N ruthenium(2+) Chemical compound [Ru+2] YAYGSLOSTXKUBW-UHFFFAOYSA-N 0.000 description 1
• FSYKKLYZXJSNPZ-UHFFFAOYSA-N sarcosine Chemical compound C[NH2+]CC([O-])=O FSYKKLYZXJSNPZ-UHFFFAOYSA-N 0.000 description 1
• 239000012056 semi-solid material Substances 0.000 description 1
• 238000012163 sequencing technique Methods 0.000 description 1
• 230000000405 serological effect Effects 0.000 description 1
• 238000004557 single molecule detection Methods 0.000 description 1
• 150000003384 small molecules Chemical class 0.000 description 1
• FHHPUSMSKHSNKW-SMOYURAASA-M sodium deoxycholate Chemical compound [Na+].C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC([O-])=O)C)[C@@]2(C)[C@@H](O)C1 FHHPUSMSKHSNKW-SMOYURAASA-M 0.000 description 1
• SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
• 235000010344 sodium nitrate Nutrition 0.000 description 1
• 239000004317 sodium nitrate Substances 0.000 description 1
• VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Inorganic materials [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 1
• 239000002689 soil Substances 0.000 description 1
• 244000000000 soil microbiome Species 0.000 description 1
• 230000003381 solubilizing effect Effects 0.000 description 1
• 235000011067 sorbitan monolaureate Nutrition 0.000 description 1
• 230000000707 stereoselective effect Effects 0.000 description 1
• 230000003335 steric effect Effects 0.000 description 1
• 230000001954 sterilising effect Effects 0.000 description 1
• 238000004659 sterilization and disinfection Methods 0.000 description 1
• 238000003756 stirring Methods 0.000 description 1
• 239000000758 substrate Substances 0.000 description 1
• 125000001273 sulfonato group Chemical group [O-]S(*)(=O)=O 0.000 description 1
• 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
• 230000004083 survival effect Effects 0.000 description 1
• 230000002194 synthesizing effect Effects 0.000 description 1
• TUNFSRHWOTWDNC-HKGQFRNVSA-N tetradecanoic acid Chemical compound CCCCCCCCCCCCC[14C](O)=O TUNFSRHWOTWDNC-HKGQFRNVSA-N 0.000 description 1
• MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 1
• 238000005382 thermal cycling Methods 0.000 description 1
• 210000003371 toe Anatomy 0.000 description 1
• XETCRXVKJHBPMK-MJSODCSWSA-N trehalose 6,6'-dimycolate Chemical compound C([C@@H]1[C@H]([C@H](O)[C@@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](COC(=O)C(CCCCCCCCCCC3C(C3)CCCCCCCCCCCCCCCCCC)C(O)CCCCCCCCCCCCCCCCCCCCCCCCC)O2)O)O1)O)OC(=O)C(C(O)CCCCCCCCCCCCCCCCCCCCCCCCC)CCCCCCCCCCC1CC1CCCCCCCCCCCCCCCCCC XETCRXVKJHBPMK-MJSODCSWSA-N 0.000 description 1
• 238000007514 turning Methods 0.000 description 1
• 210000003934 vacuole Anatomy 0.000 description 1
• 238000010200 validation analysis Methods 0.000 description 1
• 230000001018 virulence Effects 0.000 description 1
• 238000010626 work up procedure Methods 0.000 description 1
• DTOSIQBPPRVQHS-UHFFFAOYSA-N α-Linolenic acid Chemical compound CCC=CCC=CCC=CCCCCCCCC(O)=O DTOSIQBPPRVQHS-UHFFFAOYSA-N 0.000 description 1